- Open Access
Lipid droplet binding thalidomide analogs activate endoplasmic reticulum stress and suppress hepatocellular carcinoma in a chemically induced transgenic mouse model
© Nagy et al.; licensee BioMed Central Ltd. 2013
Received: 12 November 2013
Accepted: 15 November 2013
Published: 22 November 2013
Hepatocellular carcinoma (HCC) is the most frequent and aggressive primary tumor of the liver and it has limited treatment options.
In this study, we report the in vitro and in vivo effects of two novel amino-trifluoro-phtalimide analogs, Ac-915 and Ac-2010. Both compounds bind lipid droplets and endoplasmic reticulum membrane, and interact with several proteins with chaperone functions (HSP60, HSP70, HSP90, and protein disulfide isomerase) as determined by affinity chromatography and resonant waveguide optical biosensor technology. Both compounds inhibited protein disulfide isomerase activity and induced cell death of different HCC cells at sub or low micromolar ranges detected by classical biochemical end-point assay as well as with real-time label-free measurements. Besides cell proliferation inhibiton, analogs also inhibited cell migration even at 250 nM. Relative biodistribution of the analogs was analysed in native tissue sections of different organs after administration of drugs, and by using fluorescent confocal microscopy based on the inherent blue fluorescence of the compounds. The analogs mainly accumulated in the liver. The effects of Ac-915 and Ac-2010 were also demonstrated on the advanced stages of hepatocarcinogenesis in a transgenic mouse model of N-nitrosodiethylamine (DEN)-induced HCC. Significantly less tumor development was found in the livers of the Ac-915- or Ac-2010-treated groups compared with control mice, characterized by less liver tumor incidence, fewer tumors and smaller tumor size.
These results imply that these amino-trifluoro-phthalimide analogs could serve potent clinical candidates against HCC alone or in combination with dietary polyunsaturated fatty acids.
Hepatocellular carcinoma (HCC) is the fifth most common cancer of men, while the eighth most frequent cancer of women worldwide, and the second leading cause of cancer death [1, 2]. The majority of HCC cases are associated with chronic hepatitis or cirrhosis induced by persistent infection with hepatitis B or hepatitis C virus . Despite advances in different chemotherapies which are often associated with toxic side effects, liver cancer has limited treatment options. More effective therapeutic agents with fewer side effects are in the focus of current research.
Novel thalidomide analogs, Ac-915 and Ac-2010 (4-amino-substituted 2,6-diisopropylphenyl- -5,6,7-trifluoroisoindole-1,3-diones) were synthesized from starting tetrafluoro thalidomide based on our previously published synthetic approach . Both molecules possessed a strong blue fluorescence, like the previously synthesized ones , and in the present study their intracellular and tissue distribution were detected based on their fluorescent characteristics. Here, we demonstrated that Ac-915 and Ac-2010, novel amino-trifluoro-phtalimide analogs with novel substitutions also interfere with lipid droplets and the endoplasmic reticulum (ER), and induce intracellular reactive oxygen species (ROS) at lower concentrations than the previously described compounds. The novel compounds described here, specifically interact with lipid droplet-associated proteins, protein disulfide isomerase (PDI) and heat-shock proteins (HSPs) that are involved in chaperone functions. The upregulation of HSPs, as observed in various cancers, including liver cancer suggests that they might be involved in carcinogenesis [5–7]. Knockdown of PDI activity can cause accumulation of misfolded proteins in the ER, activation of apoptotic signaling, and induction of caspase-dependent apoptosis in breast cancer cells . Here we investigated the possible PDI inhibition of the novel analogs by using enzymatic assays.
Lipid droplets (LDs) are one of the main intracellular targets of amino-trifluoro-phtalimide analogs. It was shown that LD accumulation occurs in vivo in prenecrotic cancer tissues, therefore LDs can serve as in vivo markers of cancer . Imbalance in lipid homeostasis can finally lead to membrane disruption and activation of lipoapoptosis . As LDs are formed in the ER we were able to show that specific LD-binding drugs could interfere with LD homeostasis and ER-membrane integrity and could trigger apoptosis through ER stress .
Tumors, including hepatocellular carcinoma are more sensitive to ER stress and reactive oxygen species (ROS)-inducing natural compounds, such as polyunsaturated fatty acids (PUFAs) and celestrol among others [4, 11] than normal cells as their stress response is continuously engaged due to their chronic stress situation, thereby leading to activation of pro-apoptotic signals and finally tumor cell death .
Loss of lipid droplets in hepatic stellate cells is one of the first events observed in the development of liver disease leading to HCC, mostly due to the dramatic drop in cellular retinyl ester content . Surprisingly, in study Blaner and co-workers showed that Lrat KO mice, which lacks the sole enzyme responsible for hepatic retinyl ester synthesis, showed significantly less liver tumor development compared with wild-type mice, as characterized by less liver tumor incidence and smaller tumor size .
On the contrary, the liver of matrilin-2 KO (Matn2−/−) mice contained macroscopic tumors of both larger number and size than the wild-type liver after diethylnitrosoamine (DEN) treatment. DEN is widely used as a carcinogen in experimental animal models. Upon intraperitoneal administration into weaning mice at 2 weeks after birth, hepatic tumors are formed 8 month later . Since DEN itself does not exert carcinogenicity, it needs to be bioactivated by cytochrome P450 enzymes in the liver, resulting in DNA-adducts that form through an alkylation mechanism locally, which induce the formation of putative preneoplastic lesions .
Owing to the increased number and size of the DEN-induced liver tumors in the transgenic Matn2−/− mice, we used this in vivo model to assess the efficacy of our novel amino-trifluoro-phtalimide analogs. The worthwhile in vivo efficacy data presented here anticipate the completion of pre-clinical studies and initiate a clinical study on evaluating the effects of Ac-915, or the more potent Ac-2010 analog in humans with high risk for liver carcinoma.
Material and methods
Cell culture studies
Hep3B and HepG2 cells were grown in mixture of Dulbecco’s Modified Eagle Medium (D-MEM) (high glucose) (Gibco BRL, Carlsbad, CA, USA) and Nutrient Mixture F-12 Ham (Sigma, St. Louis, MO, USA) containing penicillin (50 IU/ml)–streptomycin (50 mg/ml) and 10% fetal bovine serum. For cytotoxicity assays, cells were seeded at a density of 10.000 cells per well into 96-well cell culture plates and maintained in a humidified atmosphere of 95% air and 5% CO2 for 12 h, then treated with different concentrations of Ac-2010 or Ac-915. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was applied to drug treated and control (0.2% DMSO) cells with CellTiter 96® AQueous Assay (Promega, Madison, WI) according to the manufacturer’s protocol.
HepG2 cells were cultured in glass bottom culture dishes (MatTek Corporation, Ashland, MA). ER was labeled in live cells with ER-Tracker Green (Invitrogen, Carlsbad, CA) following the manufacturer’s protocol. After staining the solution was replaced by fresh Hank’s Balanced Salt Solution with calcium and magnesium containing 5 μM Ac-915 or Ac-2010 and 5 min later the cells were visualized using an Olympus Fluoview FV1000 confocal laser scanning microscope equipped with 20x (N.A 0.75) and 40x oil (N.A 1.3) objectives. We applied a 543 nm laser for detection of the ER-Tracker Green and 405 nm laser for detection of Ac compounds .
Mice were injected i.v. with Ac-915 or Ac-2010 (20 mg/kg) and after different time points tissues were dissected, washed in PBS, embedded in Tissue-Tek® O.C.T™ medium. After fast-freezing, the samples were stored at −20°C. Tissue sections (20 μm) were prepared by using a Leica CM1950 cryostat at −20°C. From each tissue (liver, brain, kidney, muscle, heart) three independent images were recorded as above. Images were analyzed with the Olympus Fluoview 1.6 Ver Viewer software.
Affinity chromatography and protein identification
Ac-201 compound, a previously described trifluoro-amino-phtalimide analog  was covalently attached to activated controlled pore glass resins by using the AviLink™ technology (Avicor, Szeged, Hungary, http://www.avicorbiotech.com) . After blocking and washing the columns, whole cell lysate from 6×107 RVH cells was applied onto the column with 1 ml resin in PBS containing protease inhibitors. After washing the columns with 20 ml each of 0.2 M and 0.5 M NaCl in PBS, proteins were eluted with 1% SDS in PBS and applied to polyacrylamide gel-electrophoresis. Specific protein bands were cut out and stored at 4°C until further processing for mass spectrometry analysis. Samples were processed for mass spectrometry by using the UCSF in-gel digestion protocol (http://donatello.ucsf.edu/ingel.html). LC-MS analysis was performed by using the Agilent 1100 LC-MS/MS system with Zorbax 300SB-C18, 5 μm, 5mmx0,3 mm and Zorbax 300SB-C18, 3.5 μm, 150x0,075 mm columns). MS-method: Scan: m/z 300–1600, MS2 Scan: m/z 100–1800.
Waveguide optical biosensor assay
Protein immobilization was accomplished by applying 10 μL of 50 μg/mL human PDI (Sino Biological Inc., Beijing, China) or 50 μg/mL human HSP70 (Sino Biological) or 100 μg/mL human HSP90 (Sino Biological) in 20 mM sodium acetate pH 5.5 into a preactivated 384-well biochemical plate (Perkin Elmer) and incubating overnight at 4°C. The microplates were subsequently washed three times with assay buffer (PBS, pH 7.4/0.005% Tween 20/3% DMSO) and, after 25 minutes thermal equilibration, a baseline reading was taken in the Enspire Multimode Plate Reader (Perkin Elmer). Finally, 15 μL assay buffer was added including different concentrations of Ac-915 or Ac-2010. The Enspire response is measured as a shift in reflected wavelength and is expressed in picometers (pm). The affinity (KD) was calculated using a curve fitting method.
Protein disulfide isomerase assay
The assay is based on the measurement of the catalytic reduction of insulin as described by Lundstrom and Holmgren . In this assay, PDI facilitates the reduction of insulin in the presence of DTT. The reduced insulin chains aggregate, and the turbidity is monitored spectrophotometrically at 650 nm. The assay was performed in a 96-well plate format and a volume of 30 μl in the presence of 1 mM DTT, 1 μg PDI (Sino Biological), 0.14 mM bovine pancreas insulin (Sigma), and 0.2 mM EDTA in 100 mM potassium phosphate, pH 7.0. The progress of the reaction was monitored on a 96-well plate reader (Model 680 Microplate Reader, Bio-Rad Laboratories) at 650 nm for 95 minutes at 25°C. Ac-915, Ac-2010 or DMSO control was added prior to the addition of enzyme at the concentrations indicated. The nonenzymatic reduction of insulin by DTT was recorded in a control well without PDI. IC50 values of the compounds were calculated using nonlinear regression analysis.
Real-time cellular analysis with the xCELLigence System
Cytotoxicity and cell migration was monitored with the real-time cell electronic sensing, xCELLigence System (RT-CES, Acea-Roche) [20, 21]. Cytotoxicity effects of Ac-2010 and Ac-915 compounds were tested on two different hepatocellular carcinoma cell lines (HepG2 and Hep3B). The RT-CES 96-well E-plate was coated with gelatin and then washed twice with PBS. Growth media (respective to cell types) were then gently dispensed into each well of the 96-well E-plate for background readings by the RT-CES system prior to addition of cell suspension at a density of 6000 cells/well. Devices containing the cell suspension were kept at 37° in a CO2 incubator for 8 h prior to treatment with different concentrations of Ac-2010 and Ac-915. Cell growth was monitored for 48 h by measurements of electrical impedance every 5 min.
For migration assay, 160 μl of media containing 10% FBS was added to the lower chambers of CIM-plate 16. Wells of the upper chamber sealed at the bottom with a micro-pore-containing polycarbonate were filled with Hep3B cell suspension (20,000 cells/well) in 100 μl serum-free medium. Cell migration to lower chamber was monitored and expressed as cell index value. Continuous recording of impedance in cells of both systems was reflected by cell index value.
ROS generation was determined by the increase in DCFDA (2',7'-dichlorodihydrofluorescein diacetate, Sigma) fluorescence after drug stimulation. HepG2 cells were washed, resuspended in 1% bovine serum albumin in Hanks buffered saline solution (BSA-HBSS) at 106 cells/mL and maintained at 37°C for analysis. Cells were treated with the indicated drugs (Ac-915, Ac-2010). DCFDA was added 60 min prior to harvest, at 2 μM final concentration and intracellular ROS production was measured by using a fluorescence activated cell sorter (Beckton Dickinson) with 20,000 events/test. All the other parameters and calculations were as described before .
HepG2 cells (20000 cells per well in a 96-well plate) were incubated with Ac-915 in 50 μl PBS for 2 hours in a humidified atmosphere of 95% air and 5% CO2. 50 μl aliquots of prepared 2X GSH-GloTM Reagent (GSH-GloTM Glutathione assay; Promega, Madison, WI) were added to the wells and incubation continued at room temperature for 30 minutes. 100 μl of reconstituted Luciferin Detection Reagent (Promega) was added to each well and cells were incubated for 15 minutes further. Negative controls and blank reactions were also prepared. The amount of light produced was detected by luminometer.
Animal maintenance and treatments
All mice were fed a commercial diet and water ad libitum and were housed in an animal facility under a 12 h light/dark cycle at constant temperature and humidity. For all of our studies, we employed male Matn2-deficient mice (Matn2−/−) congenic in the 129/Sv genetic background .
For studies of liver tumor development, 15-day-old mice were treated with a single dose of DEN (Sigma-Aldrich, Budapest, Hungary) dissolved in saline at a dose of 25 mg/kg body weight by i.p. injection. 4 months after DEN injection mice were treated with either Ac-915 for 3 months (n = 15) or with Ac-2010 for 1 month (n = 10). Mice were killed 8 months after DEN administration for determination of tumor occurrence (number of tumor nodules) and liver mass index (liver weight/body weight × 100). Treatments were conducted by i.p. injection of Ac-915 at a dose of 10 mg/kg body weight three times a week or Ac-2010 at a dose of 4 mg/kg body weight three times a week.
The animal experiments were performed according to Institutional and National Animal Experimentation and Ethics Guidelines in possession of an ethical clearance (XVI/03047-2/2008).
In vitro effect on cell proliferation and migration
To determine whether our novel compounds have only effects on cell proliferation or they inhibit cell migration, the same technology was used. Cell migration was followed in real-time by using the RTCA DP xCELLigence System (Acea-Roche). This is a novel cell migration and invasion assay system that uses the Boyden Chamber principle but the bottom chamber has a micro-pore-containing polycarbonate membrane, which contains microelectronic sensor arrays on its bottom surface. Migration of cells is detected when cells go through these electrodes, which changes impedance, and will increase cell index. As seen in Figure 2b Ac-2010 inhibited migration even at 250 nM, where no cytotoxicity could be detected in case of HepG2 cells. At higher concentration the drug completely inhibited cell migration 2 h after administration.
Mechanism of action
Trifluoro-phtalimide-associated proteins from human cancer cells
14-3-3 protein epsilon
Cytoplasm, lipid droplet
Aldehyde dehydrogenase, mitochondrial
Mitochondrium, lipid droplet
Proteosomal degradation of proteins
Oxidation of drugs, pesticides, xenobiotics
Mitochondrium, lipid droplet
Hsp 70 kDa protein 5 (GRP 78)
ER, lipid droplet
Cytoplasm, lipid droplet
Cytoplasm, lipid droplet
Leucine-rich repeat-containing protein 59
Regulate nuclear import
Long-chain-fatty-acid-CoA ligase 3
Lipid biosynthesis, fatty acid degradation
Mitochondrium, peroxisome, microsome, lipid droplet
Membrane-associated progesterone receptor 1
Receptor for progesterone
Neutral alpha-glucosidase AB precursor
Maturation of glycoproteins
Phosphate carrier protein, mitochondrial
Degradation of prenylated proteins
Protein disulfide-isomerase A6
ER, lipid droplet
Suppression of tumorigenicity
Ras-related protein Rab-7
Endosome, lysosome, phagosome, lipid droplet
Ras-related protein Rab-10
Vesicular trafficking, neurotransmitter release
Cell membrane, lipid droplet
Ras-related protein Rab-11B
Cell membrane, lipid droplet
T-complex protein 1 subunit zeta
Motile, morphological processes, endocytosis
Tubulin beta-2 chain
Cytoskeleton, vesicular transport
Cytoplasm, lipid droplet
Elimination of toxic xenobiotics
Vesicle-trafficking protein SEC22b
ER, Golgi, lipid droplet
In Table 1 the possible function of the protein targets can be also seen. The protein target functions can be classified in three major classes: 1) vesicular and membrane trafficking, 2) chaperone functions, and 3) elimination of toxic compounds.
Direct biomolecular interactions were determined between human recombinant and purified HSP70, HSP90, PDI proteins and Ac-915 and Ac-2010 with resonant waveguide optical biosensor technology in a biochemical binding assay . The technology is based on a microplate with resonant waveguide optical biosensors integrated into each well and a high throughput screening-compatible optical reader (Enspire system, Perkin Elmer). The biochemical interaction between the small molecule and the protein was measured as a shift in reflected wavelength and is expressed in picometers (pm). The binding of both analogs to each of the protein was specific and saturable with the dose response (data not shown). The affinity (KD) values were calculated as follows for Ac-915: HSP70 KD: 14 μM, HSP90 KD: 11.5 μM and PDI KD: ≈10.5 μM; for Ac-2010: HSP70 KD: ≈16 μM, HSP90 KD: ≈16 μM and PDI KD: ≈6 μM.
The upregulation of HSPs as observed in various cancers, including liver cancer suggests that they might be involved in carcinogenesis. In particular, the enhancement of carcinogenesis via the overexpression of HSP60, HSP70 and HSP90 has been previously implicated in animal models  and in clinical samples [5–7, 24]. Because of the stressful cancerous microenvironment, tumor cells strived to increase the expression of chaperone proteins for cytoprotective function and to enhance tumor growth and metastasis, therefore inhibition of their chaperone function is a logical option for cancer treatment.
Intracellular localization studies were in good concordance with our target identification results, where most of our hits were localized to ER or lipid droplets.
Based on the affinity target identification and co-localization results, PDI was selected to study whether our novel amino-trifluoro-phtalimide analogs influence its enzymatic activity. PDI is a multifunctional 57-kDa oxidoreductase of the thioredoxin superfamily that catalyzes the formation, cleavage and rearrangement of disulfide bonds and, by acting as a molecular chaperone, it facilitates protein folding . PDI is expressed mainly in the ER of eukaryotic cells, where it predominantly forms disulfide bonds in nascent secretory proteins. It catalyzes the rearrangement of incorrect disulfide bonds through isomerase activity, thus supporting proper protein folding mainly during cellular stress, but during normal cellular conditions as well . Loss of PDI activity can cause accumulation of misfolded proteins in the ER, i.e. ER stress and subsequently induce cell death. Hashida et al. presented that PDI-knockdown activated apoptotic signaling, resulting mitochondrial cytochrome c release, activation of different caspases, and finally it induces caspase-dependent apoptosis in MCF7 cells .
As anticipated both Ac-915 and Ac-2010 inhibited the activity of PDI with about 15 μM and 9 μM IC50 values, respectively. These values are in good correlation with the KD values determined by direct biochemical interaction measurements using waveguide optical biosensors. The activity of PDI was based on the measurement of the catalytic reduction of insulin as described by Lundstrom and Holmgren . In this assay both analogs inhibited the PDI-induced reduction of insulin in the presence of dithiothreitol. The reduced insulin chains aggregated slower in the presence of the analogs, when compared to untreated samples due to the slower insulin reduction (data not shown). Although IC50 values are higher than the effective concentration inducing ROS, GSH depletion and cytotoxicity, we assume that both Ac-915 and Ac-2010 are localized in the ER and their local, subcellular concentrations could be much higher. Therefore appropriate inhibition of PDI can be achieved at relative lower concentrations applied to cells.
There is increasing evidence that ER stress plays a crucial role in the regulation of apoptosis in cancer and normal cells . ER stress leads to the production of misfolded proteins, and as a consequence to alleviate ER stress different chaperones and folding enzymes are up-regulated. During this response, several pro-apoptotic signals are activated and, depending on the extent of the ER stress, cells undergo apoptosis . Our results further support our hypothesis that our novel amino-trifluoro-phtalimide analogs interact with the ER and proteins associated with it, they induce ER stress, ROS production, decreased glutathione level and finally cell death.
From these results we concluded that the novel trifluor-amino-phtalimides indeed accumulate in the liver, the target organ of our cancer study.
In vivo effects on DEN-induced hepatocarcinoma model
Based on the increased number and size of the DEN-induced liver tumors in the transgenic Matn2−/− mice compared to the wild-type mice, the transgenic model was used to assess the in vivo effects of our potent amino-trifluor-phthalimide analogs.
To investigate whether the other analog, Ac-2010 may exhibit similar activity the same experiment was replicated, but instead of applying the drug for 3 months Ac-2010 was administered only for 1 month (n = 10). Liver samples were analyzed at the same time point, 8 months after DEN administration. Because of higher acute toxicity and better solubility (data not shown), Ac-2010 was injected at a lower dose (4 mg/kg) three times a week. Even with this protocol we obtained better result, than with Ac-915. Significantly (p < 0.01) less liver tumor incidence, fewer tumors and lower liver mass index was obtained compared to the untreated control group Figure 6c.
Drug administration was started 4 months after DEN administration, when the early stages of carcinogenesis ended and pre-neoplastic foci already appeared. Our data imply that Ac-915 or Ac-2010 treated mice experience less cell proliferation and cancer progression at later stages of liver cancer development. These findings may open a novel chemotherapeutic intervention for patients with the cancerous stage and these analogs may be useful in preventing HCC development.
HCC is the most frequent and aggressive primary tumor of the liver and it has limited treatment options. The present study demonstrated a potent cell death-inducing effect of two novel amino-trifluoro-phtalimide analogs, Ac-915 and Ac-2010. Amino-trifluoro-phthalimides, which bind lipid droplets, induce intracellular ROS formation and ER-stress . Both Ac-915 and Ac-2010 compounds induced cell death of liver cancer cells at sub or low micromolar ranges detected by classical biochemical end-point assay as well as with real-time measurements. Besides cell proliferation inhibition, analogs exert cell migration inhibition even at 250 nM. Cytotoxic effects of the novel analogs were mediated by affecting chaperone functions, induction of oxidative stress and depletion of intracellular GSH. The novel amino-trifluoro-phthalimides interacted with several proteins that localized into lipid droplets and ER. Among their candidate protein targets are the different heat-shock proteins (HSP60, HSP70, and HSP90) and protein disulfide isomerase (PDI). Direct biomolecular interactions between human HSP70, HSP90, PDI proteins and Ac-915 and Ac-2010 were confirmed with resonant waveguide optical biosensors. The activity of PDI was inhibited by both Ac-915 and Ac-2010 at micromolar concentrations.
Relative biodistribution of the analogs was analyzed in using native tissue sections of different organs after administration of drugs, and fluorescent confocal microscopy based on the inherent blue fluorescence of the compounds. The target organs of the analogs were the liver and the kidney. No, or minimal penetration could be detected into the brain, the muscle or the heart.
We used the Matn2−/− mice and DEN treatment for induction of tumors in the liver. Mice were treated either with Ac-915 (10 mg/kg) for 3 months, or Ac-2010 (4 mg/kg) for 1 months, following 4 months of DEN treatment. Liver tumor assessment was conducted 8 months after DEN administration. Significantly less tumor development was found in the livers of the Ac-915- or Ac-2010-treated groups compared with those of control mice, and were characterized by less liver tumor incidence, fewer tumors and smaller tumor size. These results indicate that treatment with either Ac-915 or with Ac-2010 affected tumor progression at later stages, which implies that these amino-trifluoro-phthalimide analogs alone or in combination with agents or natural compounds that influence ER-stress could serve potent clinical candidates against HCC.
This work was supported by the following grants: GOP-1.3.1-10/B-2010-0016, GOP-1.1.1-11-2011-0003 and GOP-1.1.1-11-2012-0015 for Avidin.
- Trevisani F, Cantarini MC, Wands JR: Recent advances in the natural history of hepatocellular carcinoma. Carcinogenesis. 2008, 29: 1299-1305.View ArticlePubMedGoogle Scholar
- El-Serag HB: Hepatocellular carcinoma. N Engl J Med. 2011, 365: 1118-1127.View ArticlePubMedGoogle Scholar
- Perz JF, Armstrong GL, Farrington LA, Hutin YJ, Bell BP: The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide. J Hepatol. 2006, 45: 529-538.View ArticlePubMedGoogle Scholar
- Puskás LG, Fehér LZ, Vizler C, Ayaydin F, Rásó E, Molnár E: Polyunsaturated fatty acids synergize with lipid droplet binding thalidomide analogs to induce oxidative stress in cancer cells. Lipids Health Dis. 2010, 9: 56-PubMed CentralView ArticlePubMedGoogle Scholar
- Joo M, Chi JG, Lee H: Expressions of HSP70 and HSP27 in hepatocellular carcinoma. J Korean Med Sci. 2005, 20: 829-834.PubMed CentralView ArticlePubMedGoogle Scholar
- Chuma M, Sakamoto M, Yamazaki K, Ohta T, Ohki M, Asaka M, Hirohashi S: Expression profiling in multistage hepatocarcinogenesis: identification of HSP70 as a molecular marker of early hepatocellular carcinoma. Hepatology. 2003, 37: 198-207.View ArticlePubMedGoogle Scholar
- Lim SO, Park SG, Yoo JH, Park YM, Kim HJ, Jang KT: Expression of heat shock proteins (HSP27, HSP60, HSP70, HSP90, GRP78, GRP94) in hepatitis B virus-related hepatocellular carcinomas and dysplastic nodules. World J Gastroenterol. 2005, 11: 2072-2079.PubMed CentralView ArticlePubMedGoogle Scholar
- Hashida T, Kotake Y, Ohta S: Protein disulfide isomerase knockdown-induced cell death is cell-line dependent and involves apoptosis in MCF-7 cells. J Toxicol Sci. 2011, 1: 1-7.View ArticleGoogle Scholar
- Quintero M, Cabanas ME, Arus C: A possible cellular explanation for the NMR-visible mobile lipid (ML) changes in cultured C6 glioma cells with growth. Biochim Biophys Acta. 2007, 1771: 31-44.View ArticlePubMedGoogle Scholar
- Borradaile NM, Han X, Harp JD, Gale SE, Ory DS, Schaffer JE: Disruption of endoplasmic reticulum structure and integrity in lipotoxic cell death. J Lipid Res. 2006, 12: 2726-2737.View ArticleGoogle Scholar
- Wang WB, Feng LX, Yue QX, Wu WY, Guan SH, Jiang BH: Paraptosis accompanied by autophagy and apoptosis was induced by celastrol, a natural compound with influence on proteasome, ER stress and Hsp90. J Cell Physiol. 2012, 227: 2196-2206.View ArticlePubMedGoogle Scholar
- Faragó N, Fehér LZ, Kitajka K, Das UN, Puskás LG: MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes. Lipids Health Dis. 2011, 10: 173.PubMed CentralView ArticlePubMedGoogle Scholar
- Blaner WS, O’Byrne SM, Wongsiriroj N, Kluwe J, D’Ambrosio DM, Jiang H: Hepatic stellate cell lipid droplets: a specialized lipid droplet for retinoid storage. Biochim Biophys Acta. 2009, 1791: 467-473.PubMed CentralView ArticlePubMedGoogle Scholar
- Shirakami Y, Gottesmann ME, Blaner WS: Diethylnitrosamine-induced hepatocarcinogenesis is suppressed in lecithin: retinol acyltransferase-deficient mice primarily through retinoid actions immediately after carcinogen administration. Carcinogenesis. 2012, 33: 268-274.PubMed CentralView ArticlePubMedGoogle Scholar
- Goldfarb S, Pugh TD, Koen H, He YZ: Preneoplastic and neoplastic progression during hepatocarcinogenesis in mice injected with diethylnitrosamine in infancy. Environ Health Perspect. 1983, 50: 149-161.PubMed CentralView ArticlePubMedGoogle Scholar
- Verna L, Whysner J, Williams GM: N-nitrosodiethylamine mechanistic data and risk assessment: bioactivation, DNA-adduct formation, mutagenicity, and tumor initiation. Pharmacol Ther. 1996, 71: 57-81.View ArticlePubMedGoogle Scholar
- Molnár E, Kuntam S, Cingaram PK, Peksel B, Suresh B, Fábián G: Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery. Molecules. 2013, 20: 9999-10013.View ArticleGoogle Scholar
- Molnár E, Fábián G, Klem J, Darula Z, Hunyadi-Gulyás E, Medgyesi A: Removal of nonspecific binding proteins from cell and tissue extracts using 2-aminobenzimidazole-tethered affinity resin. Pharmazie. 2011, 66: 662-665.PubMedGoogle Scholar
- Lundstrom J, Holmgren A: Protein disulfide-isomerase is a substrate for thioredoxin reductase and has thioredoxin-like activity. J Biol Chem. 1990, 265: 9114-9120.PubMedGoogle Scholar
- Ózsvári B, Puskás LG, Nagy LI, Kanizsai I, Gyuris M, Madácsi R: A cell-microelectronic sensing technique for the screening of cytoprotective compounds. Int J Mol Med. 2010, 25: 525-530.PubMedGoogle Scholar
- Kürti L, Veszelka S, Bocsik A, Dung NT, Ozsvári B, Puskás LG: The effect of sucrose esters on a culture model of the nasal barrier. Toxicol In Vitro. 2012, 26: 445-454.View ArticlePubMedGoogle Scholar
- Mátés L, Nicolae C, Mörgelin M, Deák F, Kiss I, Aszódi A: Mice lacking the extracellular matrix adaptor protein matrilin-2 develop without obvious abnormalities. Matrix Biol. 2004, 23: 195-204.View ArticlePubMedGoogle Scholar
- Gauglitz G, Proll G: Strategies for label-free optical detection. Adv Biochem Eng Biotechnol. 2008, 109: 395-432.PubMedGoogle Scholar
- Luk JM, Lam CT, Siu AF, Lam BY, Ng IO, Hu MY: Proteomic profiling of hepatocellular carcinoma in Chinese cohort reveals heat-shock proteins (HSP27, HSP70, GRP78) up-regulation and their associated prognostic values. Proteomics. 2006, 6: 1049-1057.View ArticlePubMedGoogle Scholar
- Freedman RB: Protein disulfide isomerase: multiple roles in the modification of nascent secretory proteins. Cell. 1989, 57: 1069-1072.View ArticlePubMedGoogle Scholar
- Noiva R: Protein disulfide isomerase: the multifunctional redox chaperone of the endoplasmic reticulum. Semin Cell Dev Biol. 1999, 10: 481-493.View ArticlePubMedGoogle Scholar
- Ge Y, Montano I, Rustici G, Freebern WJ, Haggerty CM, Cui W: Selective leukemic-cell killing by a novel functional class of thalidomide analogs. Blood. 2006, 108: 4126-4135.PubMed CentralView ArticlePubMedGoogle Scholar
- Moenner M, Pluquet O, Bouchecareilh M, Chevet E: Integrated endoplasmic reticulum stress responses in cancer. Cancer Res. 2007, 67: 10631-10634.View ArticlePubMedGoogle Scholar
- Wu J, Kaufman RJ: From acute ER stress to physiological roles of the unfolded protein response. Cell Death Differ. 2006, 13: 374-384.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.