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Upregulation of triglyceride synthesis in skeletal muscle overexpressing DGAT1
Lipids in Health and Disease volume 12, Article number: 63 (2013)
The gene encoding diacylglycerol acyltransferase (DGAT1) is a functional and positional candidate gene for milk and intramuscular fat content. A bovine DGAT1 overexpression vector was constructed containing mouse MCK promoter and bovine DGAT1 cDNA. MCK-DGAT1 transgene in FVB mice was researched in present study. The transgene DGAT1 had a high level of expression in contrast to the endogenous DGAT1 in posterior tibial muscle of the transgenic mice, but a low expression level in the cardiac muscle. Compared with wild-type mice, triglyceride and DGAT1 content were approximately fourfold and 50% increased in posterior tibial muscle of the transgenic mice, respectively, while a little increase in cardiac muscle.
Triacylglycerols (TG) are quantitatively the most important storage form of energy for eukaryotic cells. Diacylglycerol acyltransferase 1 (DGAT1) encodes acyl CoA:diacylglycerol acyltransferase (DGAT) which catalyzes the terminal and only committed step in triacylglycerol synthesis . DGAT1 is present in all cell types but is most highly expressed in tissues and organs where triglyceride synthesis is most active, including adipose tissue, liver, skeletal muscle and small intestine. Bovine DGAT1 spaning 14117 base pairs (bp) was assigned to a region on bovine chromosome 14 close to the centromere and contains 17 exons and 16 introns . Its coding sequence (CDS) spans 1470 bp and encodes 489 amino acids. DGAT deficiency alters triglyceride metabolism in mammary gland of mice . Thaller et al.  treated DGAT1 as a positional and functional candidate gene for intramuscular fat deposition in cattle. Myocellular overexpression of DGAT1 resulted in increased intramyocellular TG levels but decreased myocellular DAG and ceramide levels .
Muscle creatine kinase (MCK) is critical to the energy metabolism of skeletal muscle as the key enzyme in the phosphorylcreatine shuttle between mitochondria and the myofibrils . The MCK gene is transcriptionally activated during striated muscle differentiation and is expressed at high levels in skeletal and cardiac muscles. Transcriptional regulation of the gene in cultured skeletal and cardiac muscle cells has been shown, as well as in transgenic mice . Jaynes et al.  demonstrated a 3.3 kilobases (kb) 5′-flanking region of the MCK gene which was sufficient to confer transcriptional regulation to a heterologous structural gene (chloramphenicol acetyl transferase). The major effector of high-level expression was found to have the properties of a transcriptional enhancer. This element, located between 1050 and 1256 bp upstream of the transcription start site, activated either the MCK promoter or heterologous promoters in differentiated muscle cells . Besides the 206 bp MCK enhancer, the 1 kb region of DNA between the enhancer and the basal promoter is essential for the transgenic expression in skeletal muscle ,unlike its behavior in cell culture . Long terminal repeat (LTR) inserted with MCK enhancers was utilized by Wang et al.  to increase human factor IX (FIX) expression in skeletal muscle cells in vitro and in vivo.
In the present study, we wanted to investigate whether bovine DGAT1 could express in skeletal muscle of mice under the control of mouse MCK promoter, and determine the effect of bovine DGAT1 overexpression on triglyceride synthesis and storage in MCK-DGAT1 transgenic mice.
Overexpression of DGAT1 in skeletal muscle of MCK-DGAT1 transgenic mice
We used the 1.38 kb mouse MCK promoter, which is widely used for skeletal and cardiac muscle transgene expression, to direct the expression of a full-length bDgat1 cDNA. The RT gene expression analysis was performed on five transgenic mice and five WT mice, which showed that expression of the transgene was primarily restricted to skeletal muscle, and had a high level contrast to the endogenous DGAT1 in posterior tibial muscle of the transgenic mice (Figure 1A), but a very low expression level in the cardiac muscle (Figure 1B). Gene expression in one WT mice were showed in Figure 1C and Figure 1D.
Increased TG and DGAT1 content in skeletal muscle of MCK-DGAT1 transgenic mice
10 – 12 weeks mice were used in this study. No significant difference in body weight was detected between the transgenic (19.57 ± 1.04 g) and WT (20.03 ± 1.31 g) mice. TG levels in posterior tibial muscle were approximately fourfold increased in MCK-DGAT1 mice compared with WT mice (Figure 2A). A approximately 50% increase in total DGAT content was observed in posterior tibial muscle of the transgenic mice, compared with WT mice (Figure 2B). TG (Figure 2C) and DGAT1 (Figure 2D) content in cardiac muscle of MCK-DGAT1 mice were only a little higher than that of WT mice.
MCK-DGAT1 transgenic mice were healthy and fertile. The present study shows that bovine DGAT1 could express in skeletal muscle of mice under the control of mouse MCK promoter, and overexpression of DGAT1 resulted in increased TG levels in skeletal muscle of mice, while the influence of transgene on cardiac muscle was low. Liu et al.  demonstrated similar results in skeletal muscles including gastrocnemius, soleus, and posterior tibial muscles, and heart. Their results also showed the effect of DGAT1 on muscle insulin sensitivity and that DGAT1 transgenic mice were resistant to HFD (high fat diet) – induced obesity. Thus, the overexpression of DGAT1 is theoretically a possible means to protect the healthy of transgenic animals while increasing intramuscular fat.
Intramuscular fat (percentage of lipid content of muscle), also subjectively assessed as marbling, represents an important beef quality trait . The MCK-DGAT1 transgene is expected to applying in bovine to improve the quality of beef. In the subsequent study, we will investigate whether the mouse MCK promoter works in bovine.
Liu et al.  first demonstrated in vivo that although skeletal muscle of MCK-DGAT1 mice was more resistant to developing HFD-induced lipotoxicity, their liver was as susceptible for lipotoxic insulin resistance as that of wild-type mice. In our study, fatty liver was not observed in transgenic mice. Further researches will performed in subsequent study.
Generation of MCK-DGAT1 transgenic mice
The transgene contains, from the 5′-end to the 3′-end, an 1.38 kb mouse MCK promoter, and an 1.48 kb full-length bovine DGAT1 (bDGAT1) cDNA (Figure 3). Transgenic mice were produced by pronuclear injection of FVB fertilized eggs with the transgene. Genotyping was carried out by PCR using genomic DNA extracted from mouse tails. The PCR was performed with primers F (5′- GATCCCCTATGGTGCACTCT -3′) and R (5′- TACGTCTCCGTCCTTGTCTG -3′) that amplified an 1.8 kb fragment in MCK-DGAT1 mice.
Wild-type (WT) mice were purchased. Mice were housed in a barrier facility with 12-hour light/12-hour dark cycles and fed normal chow.
Reverse transcriptase PCR
Total RNA was extracted from cardiac and posterior tibial muscle using TRIzol Reagent purchased from Invitrogen. The muscle were first homogenized with TRIzol Reagent, then RNA was separated in chloroform, precipitated by isopropanol, and washed by 75% ethanol, lastly the RNA was dissolved in RNase-free water. Total RNA was reverse-transcribed into cDNA which was used in the subsequent PCR amplification. The mRNA levels were quantified using the following primers: mouse endogenous DGAT1, forward, 5′- GCCCAAGGTAGAAGAGGACGAG-3′, reverse, 5′- AGTATGATGCCAGAGCAAACACG-3′, PCR temperature program, 95°C 3 min, then 95°C 30 s, 59°C 30 s, 72°C 30 s for 30 cycles, 72°C 5 min; transgene DGAT1, forward, 5′- AGAGGAGGTGCGGGATGTGG-3′, reverse, 5′- TCGCGGTAGGTCAGGTTGTCG-3′, PCR temperature program, 95°C 3 min, then 95°C 30 s, 61°C 30 s, 72°C 30 s for 30 cycles, 72°C 5 min. mRNA levels were then expressed as ratios relative to Gapdh, which express stably in this test, forward, 5′- GATGCCCCCATGTTTGTGAT-3′, reverse, 5′- GGGTGGTCCAGGGTTTCTTA-3′, PCR temperature program, 95°C 3 min, then 95°C 30 s, 54°C 30 s, 72°C 30 s for 30 cycles, 72°C 5 min. The RT gene expression analysis was performed on five transgenic mice and five WT mice.
Tissues were homogenized by pestles in isoamyl alcohol. Triglyceride mass in lipid extracts was enzymatically determined with a colorimetric kit.
Tissues were homogenized by pestles in PBS. Total DGAT1 content was measured with a DGAT1 ELISA kit. Double antibody sandwich ELISA is used in this kit to measure the content of DGAT1 in tissue homogenate quantitatively.
Data were expressed as means ± SD. Statistical differences were analyzed by ANOVA to determine overall treatment effects including TG and DGAT1 content (SPSS 11.0). A two-tailed P value of 0.01 was used to indicate statistical significance.
The experiment was conducted according to protocols approved by the Institutional Animal Care and Use Committee at Tianjin Agriculture University.
Zhuying Wei Joint first author.
Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E, Novak S, Collins C, Welch CB, Lusis AJ: Identification of a gene encoding an acyl CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Cell Biol. 1998, 95: 13018-13023.
Winter A, Kramer W, Werner FAO, Kollers S, Kata S, Durstewitz G, Buitkamp J, Womack JE, Thaller G, Fries R: Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. PNAS. 2002, 99: 9300-9305.
Smith SJ, Cases S, Jensen DR, Chen HC, Sande E, Tow B, Sanan DA, Raber J, Eckel RH, Farese RV: Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat. Nat Genet. 2000, 25: 87-90.
Thaller G, Kramer W, Winter A, Kaupe B, Erhardt G, Fries R: DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Anim Genet. 2003, 34: 354-357.
Liu L, Zhang Y, Chen N, Shi X, Tsang B, Yu YH: Upregulation of myocellular DGAT1 augments triglyceride synthesis in skeletal muscle and protects against fat-induced insulin resistance. J Clin Invest. 2007, 117: 1679-1689.
Bessman SP, Geiger PJ: Transport of energy in muscle: the phosphorylcreatine shuttle. Science. 1981, 211: 448-452.
Johnson JE, Wold BJ, Hauschka SD: Muscle creatine kinase sequence elements regulating skeletal and cardiac muscle expression in transgenic mice. Mol Cell Biol. 1989, 9: 3393-3399.
Jaynes JB, Chamberlain JS, Buskin JN, Johnson JE, Hauschka SD: Transcriptional regulation of the muscle creatine kinase gene and regulated expression in transfected mouse myoblasts. Mol Cell Biol. 1986, 6: 2855-2864.
Jaynes JB, Johnson JE, Buskin JN, Gartside CL, Hauschka SD: The muscle creatine kinase gene is regulated by multiple upstream elements, including a muscle-specific enhancer. Mol Cell Biol. 1988, 8: 62-70.
Donoviel DB, Shield MA, Buskin JN, Haugen HS, Clegg CH, Hauschka SD: Analysis of muscle creatine kinase gene regulatory element in skeletal and cardiac muscle of transgenic mice. Mol Cell Biol. 1996, 16: 1649-1658.
Wang JM, Hou J, Qiu XF, Kotoku K, Xue JL: Hybrid retroviral vector with MCKenhancers inserted in LTR for stable and specific expression of human factor IX in skeletal muscle. Chinese Med J. 2004, 117 (6): 893-898.
Liu L, Shi X, Choi CS, Shulman GI, Klaus K, Nair KS, Schwartz GJ, Zhang Y, Goldberg IJ, Yu YH: Paradoxical Coupling of Triglyceride Synthesis and Fatty Acid Oxidation in Skeletal Muscle Overexpressing DGAT1. Diabetes. 2009, 58: 2516-2524.
This work was supported by grant number 2008zx08007--002 and 2011zx08007--002 from National transgenic project.
The authors declare that they have no competing interests.
FY carried out the whole experiment of the studies and drafted the manuscript. ZW, GL, HG and GS participated in the design of the study. XD, XL and XG participated in the experiment and helped to the data analysis. All authors read and approved the final manuscript.
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Yang, F., Wei, Z., Ding, X. et al. Upregulation of triglyceride synthesis in skeletal muscle overexpressing DGAT1. Lipids Health Dis 12, 63 (2013). https://doi.org/10.1186/1476-511X-12-63
- Transgenic mice
- Intramuscular fat content