- Open Access
Low HDL cholesterol is correlated to the acute ischemic stroke with diabetes mellitus
© Luo et al.; licensee BioMed Central Ltd. 2014
- Received: 22 August 2014
- Accepted: 21 October 2014
- Published: 14 November 2014
To clarify the role of lipid composition in the occurrence of acute ischemic stroke (AIS) with diabetes mellitus (DM) and its influence factors.
Data was collected from the patients hospitalization in Affiliated Drum Tower Hospital of Nanjing University Medical School from October 2008 to May 2012, which included AIS and non-AIS consist of transient ischemic attack (TIA) and Vertigo or dizzy. Lipid and other risk factors including blood glucose (BG), uric acid (UA), hypertension, DM and atrial fibrillation (AF) were investigated in relation to occurrence of AIS.
The level of high density lipoprotein (HDL) cholesterol was decreased obviously in the DM group compared to the non-DM group and low level of HDL cholesterol was prevalent in the AIS patients with DM. logistic regression demonstrated that decreased HDL cholesterol was correlated to the AIS with DM, not all AIS, and the relative risk of ischemic stroke in low HDL cholesterol level group was 2.113 (95% CI = 1.191-3.749, P = 0.011) compared to the high level group. Furthermore, age has the obviously impact on it. HDL cholesterol was correlated to the AIS with DM just in the populations of aged ≦70 years (OR = 0.192, P = 0.000), low level of HDL cholesterol had more high risk of ischemic stroke than that in the high level group (OR = 6.818, P = 0.002).
Decreased HDL cholesterol was correlated to the occurrence of AIS with DM, especially in the populations of aged ≦70 years.
- Diabetes mellitus
- Ischemic stroke
- High density lipoprotein cholesterol
Diabetes mellitus (DM) is considered as one of the important risk factors of acute ischemic stroke (AIS), which has been proved in a series of studies including our previous report. The important pathogenesy behind it is atherosclerosis (AS)[2, 3], which perhaps has more direct correlation with the blood lipid. As the independent risk factors for cardiovascular and cerebrovascular disease, both of the DM and blood lipid have the impact on the AIS which is accepted wildly, but the relation between the DM and lipid during the occurrence and development of AIS is unclear.
In the previous studies about AS and AIS, among the lipid compositions, more attentions were paid to the role of low density lipoprotein cholesterol (LDL cholesterol). High level of LDL cholesterol was considered to be a predictor of cardiovascular and cerebrovascular diseases(CVD) in the general population. But in the past several years recently, the role of high density lipoprotein cholesterol (HDL cholesterol) was continued to be known. The relation between HDL cholesterol and the risk of ischemic stroke was inconsistent. In the current opinions, more studies supported an inverse association between HDL cholesterol and the risk of ischemic stroke[5, 6], despite this was not confirmed in the populations of Asia.
Diabetic individuals, as a special population with a 2- to 3-fold increase in the risk of stroke than the general population, controlling of the blood glucose failed to reduce the incidence of AIS[8–10]. If there any things can we do to prevent the occurrence of AIS, risk factors for AIS in diabetic individuals were not fully known until now. Patients with DM have significantly increased serum triglyceride (TG) and decreased HDL cholesterol concentrations, especially the latter, which plays a major role in the progression of atherosclerosis (This also has been confirmed in our previous article, in Chinese). Based on this background, we hypothesis that HDL cholesterol may participate in the course of AIS induced by DM. The purpose of present study was to evaluate the role of HDL cholesterol on the risk of AIS events in the population of DM and its relative influence factors.
Data for this retrospective study was collected from the hospitalization patients of department of neurology in Affiliated Drum Tower Hospital of Nanjing University Medical School from October 2008 to May 2012, which included AIS within 7 days of symptom onset, transient ischemic attack (TIA) and vertigo or dizzy. Here, both of the latter were set as the control represented the non-AIS population. The study was approved by institutional committee of Affiliated Drum Tower Hospital of Nanjing University Medical School. Patients who were found with pre-stroke impairment or insulin-dependent diabetes mellitus and vertigo or dizzy with organic diseases were excluded. At admission, plain CT scan of the head was done to rule out haemorrhage and MRI was done to identify the new infarction, otherwise such patients would also be excluded.
Definition of vascular risk factors
Hypertension and diabetes mellitus were defined as participants with history of relative disease or new diagnosis according to the China hypertension and DM standard (just non-insulin-dependent diabetes were included), while atrial fibrillation (AF) was defined as participants with history of AF or new diagnosis by electrocardiogram.
Blood collection and analysis
Venous blood was collected following overnight fasting for at least 12 hours, and analyzed by a solid-phase chemiluminescent immunometric assay on Immulite 2000 with the manufacturer’s reagents as directed to detect blood glucose (BG), uric acid (UA), Triglyceride (TG), total cholesterol (TC), High density lipoprotein cholesterol (HDL cholesterol), Low density lipoprotein cholesterol (LDL cholesterol).
Statistical analyses were performed with SPSS 10.0 software. The results are expressed as constituent ratio for categorical variables (χ2 test) and as mean ± SEM for the continuous variables (t-test) depending on their normal distribution. The level-risk relationship was expressed as an OR, with a corresponding 95% CI, through logistic regression. Level of significance for statistical purposes was stated at p < 0.05.
610 patients with AIS were included in the trial, among them, 385 were male and 225 were female, whose age range from 15 to 92. 202 patients had DM, 434 patients had hypertension and 81 patients had AF coexistence with AIS. While 225 non-AIS patients were set as the control group, which contained 59 TIA patients and 166 patients with vertigo or dizziness, with age- and sex-comparable to the AIS group.
Low level of HDL cholesterol prevalence in the DM group
Lipid levels in AIS patients and controls
Comparison of lipid composition between with or without DM of the AIS or non-AIS patients
non-DM(n = 408)
1.470 ± 0.050
4.812 ± 0.048
1.182 ± 0.019
2.549 ± 0.035
AIS(n = 610)
DM(n = 202)
1.582 ± 0.066
4.774 ± 0.080
1.067 ± 0.023
2.591 ± 0.058
non-DM(n = 350)
1.532 ± 0.056
4.803 ± 0.052
1.159 ± 0.019
2.551 ± 0.038
AF(n = 529)
DM(n = 179)
1.647 ± 0.072
4.832 ± 0.084
1.056 ± 0.023
2.638 ± 0.061
non-DM(n = 131)
1.442 ± 0.073
4.702 ± 0.089
1.163 ± 0.028
2.460 ± 0.070
DM(n = 35)
1.487 ± 0.098
4.359 ± 0.127
1.109 ± 0.059
2.237 ± 0.091
(n = 166)
non-DM(n = 38)
1.519 ± 0.169
4.251 ± 0.216
1.150 ± 0.044
2.313 ± 0.124
TIA(n = 59)
DM(n = 21)
1.171 ± 0.091
4.278 ± 0.144
1.005 ± 0.044
2.387 ± 0.130
non-DM(n = 169)
1.459 ± 0.068
4.601 ± 0.085
1.160 ± 0.024
2.427 ± 0.061
n = 225)
DM(n = 56)
1.368 ± 0.073
4.329 ± 0.095
1.070 ± 0.040
2.293 ± 0.075
non-DM(n = 577)
1.467 ± 0.041
4.750 ± 0.042
1.176 ± 0.015
2.513 ± 0.031
All(n = 835)
DM(n = 258)
1.535 ± 0.054
4.677 ± 0.067
1.068 ± 0.020
2.526 ± 0.049
Decreased HDL cholesterol correlated to the AIS with DM
Relative risk of AIS coexistence with DM versus risk factors
There has no correlation between HDL cholesterol and AIS
Relative risk of AIS versus risk factors
0.003-8.1 × 108
Age has the impact on the HDL cholesterol between with and without DM in AIS
Influence factors of HDL cholesterol in the AIS between with or without DM
≦60(n = 198)
61-70(n = 145)
71-80(n = 183)
>80(n = 84)
1.164 ± 0.035
1.166 ± 0.036
1.225 ± 0.034
1.172 ± 0.041
0.992 ± 0.034
0.993 ± 0.041
1.149 ± 0.040
1.151 ± 0.089
Male(n = 385)
Female(n = 225)
1.103 ± 0.023
1.327 ± 0.029
1.006 ± 0.026
1.159 ± 0.040
Yes(n = 434)
No(n = 176)
1.149 ± 0.020
1.248 ± 0.039
1.073 ± 0.026
1.043 ± 0.050
Yes(n = 81)
No(n = 529)
1.322 ± 0.056
1.159 ± 0.019
1.156 ± 0.092
1.056 ± 0.023
Low level of HDL cholesterol prevalence in the populations of ≦70 years patients with diabetes and AIS
Decreased HDL cholesterol correlated to the AIS with DM just in the populations of aged ≦70 years
Relative risk of AIS coexistence with DM versus risk factors in age subgroup
≦70 years old(n = 343)
>70 years old(n = 267)
OR (95% CI)
OR (95% CI)
DM is characterized by hyperglycaemia, but aggressive management of glucose has failed to reduce the incidence of AIS[8–10], which demonstrated that the level of glucose is not the key regulator of the AIS in patients with DM. Also, DM is usually accompany with low plasma HDL cholesterol levels, especially in non-insulin-dependent diabetes, while this may not have a direct relationship to degree of glucose control[13, 14]. We wonder whether this kind of low level of HDL cholesterol is correlated to the occurrence of AIS in the DM individuals. In this study, we identified our previous hypotheses, and found that decreased HDL cholesterol was correlated to the occurrence of AIS with DM, which mainly existed in the populations of aged ≦70 years.
According with the previous studies, our finding revealed that low level of HDL cholesterol was prevalent in the DM group, and extended this kind of trend in the populations of AIS and non-AIS. The level of HDL cholesterol was markedly reduced in the populations of diabetes mellitus compared with the non-diabetic controls, which was also confirmed in our study. But in the subgroups, we could see the obvious difference. There was no change of HDL cholesterol between diabetes and non-diabetes in the patients with vertigo or dizziness, which have the cerebrovascular causes only about 4%-6%. But decreased HDL cholesterol has already been seen in the TIA patients, and the manifestation was more prominent in the AIS populations. This seemed to suggested that low level of HDL cholesterol was just existed in the populations of ischemic cerebrovascular or atherosclerotic disease induced by DM, not simple the patients with diabetes.
The association between HDL cholesterol and ischemic stroke was unclear. Generally, an inverse association was supported by a series of prospective cohort studies[5, 6], while no association was reported by the Asia Pacific region Cohort Study and Women’s Health Study. Different results may attribute to define of the target populations. Our finding was consistent with the latter, we did not find any significant association between HDL cholesterol and the risk of total ischemic stroke, but this kind of inverse association was existed between HDL cholesterol and the risk of AIS with DM and low level group of HDL cholesterol had more high risk in the occurrence of AIS with DM than that in the high level group. The exact mechanism behind it was unclear.
Studies on lipid levels and the risk of ischemic stroke were inconsistent. In the past several decades, the main emphases were played on the total cholesterol, HDL cholesterol and LDL cholesterol. As mentioned above, low level of HDL cholesterol was the independent risk factor of AIS with DM, nor total AIS, which just represented our opinion. About the total cholesterol and LDL cholesterol, more evidences demonstrated that increased risk of AIS was associated with increased total cholesterol and LDL cholesterol levels[16–20], while other studies found there had no clear association[21, 22]. In the current study, we found that patients with AIS had high levels of TC and LDL cholesterol which was correlated to the increased risk of ischemic stroke, further identified the previous main reports.
High level of LDL cholesterol was considered as a predictor of ischemic stroke. LDL cholesterol was correlated to the AIS which was confirmed by our present study, strict control of LDL cholesterol was recommended in the prevention of ischemic cerebrovascular disease by many guidelines. But in the development of AIS with DM, we did not find any association between it and LDL cholesterol, while HDL cholesterol played the important role during its whole course. Many guidelines to prevent atherothrombotic diseases also recommend strict control of LDL cholesterol in patients with diabetes, should we keep on doing it, or transfer our focus to HDL cholesterol. To make this decision, more studies needed to be done. Perhaps, HDL cholesterol is a new therapeutic target in AIS induced by type 2 diabetes.
There are many major determinants for the risk of ischemic stroke, such as age, gender, hypertension, diabetes, lipid and AF. Lipid levels changed obviously in the course of AIS, also diabetes has been proved to have impact on it. What about other factors? Few studies have been done in this field. The association between HDL cholesterol and the risks of ischemic stroke had no difference in varied gender, so did with the hypertension or not, but the age had the impact on association between HDL cholesterol and the risks of ischemic stroke in diabetic individuals. About the AF, there had no relative reports until now. According with the previously study, we did find the age had the impact on the the level of HDL cholesterol in the AIS populations. That was, lower level of HDL cholesterol was existed in DM group compared to non-DM group both in the populations of AIS with aged ≦60 and 61–70 years, but not 71–80 and >80 years. This might to be the reason of patients with diabetes and ischemic stroke are younger, especially in those patients less than 65 years.
Further analyses demonstrated that the populations of aged ≦70 years had higher percent of low level of HDL cholesterol than that in >70 years in the diabetic individuals while not in the non-diabetic individuals. It seemed to suggest that patients with diabetes and aged ≦70 years were more prone to have decreased HDL cholesterol which was correlated to the occurrence of AIS, and we identified it. Decreased HDL cholesterol was the independent risk factor of AIS with DM just in the populations of aged ≦70 years, nor >70 years. In the report of Hayashi T et al., decreased HDL cholesterol was a risk factor for CVD in elderly patients with diabetes subjects including those aged >65 years, especially >75 years. There seemed to be some inconsistence, partly for the difference of age division, others for the difference of analytic populations or else. In our study, more attentions were paid to the decline of HDL cholesterol, based on which can we next analyze the risk relative degree of AIS or AIS with DM. In those aged >70 years, we did not find too much low levels of HDL cholesterol, whenever diabetes or non-diabetes. On the other hand, decreased HDL cholesterol was prevalent in patients with diabetes which played the important role in the AIS, obviously in the populations of aged ≦70 years. Relative results need more studies to confirm.
In summary, our current study provided the evidence that low level of HDL cholesterol was correlated to the occurrence of AIS induced by DM, while other lipid compositions had no impact on it. More important, age had the impact on it. That was, only the populations of aged ≦70 years had this kind of trend. To prevent the occurrence of ischemic stroke in diabetic individuals, more attentions should be paid to the decline of HDL cholesterol, especially those aged ≦70 years.
This work was supported by the Fundamental Research Funds for the Central Universities (021414340051) and Medical Science and technology development Foundation, Nanjing Department of Health (YKK13058).
- Luo Y, Li JW, Wang C, Guan DN, Xu Y: Diabetes mellitus influence the pathogenesy of cerebral infarction association with carotid artery plaque. Chin J Neuromed. 2012, 11: 9-12. (article in Chinese),Google Scholar
- Beckman JA, Creager MA, Libby P: Diabetes and atherosclerosis: epidemiology, pathophysiology, and management. JAMA. 2002, 287: 2570-2581. 10.1001/jama.287.19.2570View ArticlePubMedGoogle Scholar
- Hewitt J, Castilla Guerra L, Fernández-Moreno Mdel C, Sierra C: Diabetes and stroke prevention: a review. Stroke Res Treat. 2012, 2012: 673187-PubMed CentralPubMedGoogle Scholar
- Yu XH, Fu YC, Zhang DW, Yin K, Tang CK: Foam cells in atherosclerosis. Clin Chim Acta. 2013, 424: 245-252.View ArticlePubMedGoogle Scholar
- Zhang Y, Tuomilehto J, Jousilahti P, Wang Y, Antikainen R, Hu G: Total and high-density lipoprotein cholesterol and stroke risk. Stroke. 2012, 43: 1768-1774. 10.1161/STROKEAHA.111.646778View ArticlePubMedGoogle Scholar
- Amarenco P, Labreuche J, Touboul PJ: High-density lipoprotein-cholesterol and risk of stroke and carotid atherosclerosis: a systematic review. Atherosclerosis. 2008, 196: 489-496. 10.1016/j.atherosclerosis.2007.07.033View ArticlePubMedGoogle Scholar
- Woodward M, Barzi F, Feigin V, Gu D, Huxley R, Nakamura K, Patel A, Ho S, Jamrozik K, : Associations between high-density lipoprotein cholesterol and both stroke and coronary heart disease in the Asia Pacific region. Eur Heart J. 2007, 28: 2653-2660. 10.1093/eurheartj/ehm427View ArticlePubMedGoogle Scholar
- Group C, Turnbull FM, Abraira C, Anderson RJ, Byington RP, Chalmers JP, Duckworth WC, Evans GW, Gerstein HC, Holman RR, Moritz TE, Neal BC, Ninomiya T, Patel AA, Paul SK, Travert F, Woodward M: Intensive glucose control and macrovascular outcomes in type 2 diabetes. Diabetologia. 2009, 52: 2288-2298. 10.1007/s00125-009-1470-0View ArticleGoogle Scholar
- Mattila TK, de Boer A: Influence of intensive versus conventional glucose control on microvascular and macrovascular complications in type 1 and 2 diabetes mellitus. Drugs. 2010, 70: 2229-2245. 10.2165/11585220-000000000-00000View ArticlePubMedGoogle Scholar
- Ray KK, Seshasai SR, Wijesuriya S, Sivakumaran R, Nethercott S, Preiss D, Erqou S, Sattar N: Effect of intensive control of glucose on cardiovascular outcomes and death in patients with diabetes mellitus: a meta-analysis of randomised controlled trials. Lancet. 2009, 373: 1765-1772. 10.1016/S0140-6736(09)60697-8View ArticlePubMedGoogle Scholar
- Mooradian AD: Dyslipidemia in type 2 diabetes mellitus. Nat Clin Pract Endocrinol Metab. 2009, 5: 150-159. 10.1038/ncpendmet1066View ArticlePubMedGoogle Scholar
- Biesbroeck RC, Albers JJ, Wahl PW, Weinberg CR, Bassett ML, Bierman EL: Abnormal composition of high density lipoproteins in non-insulin-dependent diabetics. Diabetes. 1982, 31: 126-131. 10.2337/diab.31.2.126View ArticlePubMedGoogle Scholar
- Calvert GD, Graham JJ, Mannik T, Wise PH, Yeates RA: Effects of therapy on plasma-high-density-lipoprotein-cholesterol concentration in diabetes mellitus. Lancet. 1978, 2: 66-68.View ArticlePubMedGoogle Scholar
- Kennedy AL, Lappin TR, Lavery TD, Hadden DR, Weaver JA, Montgomery DA: Relation of high-density lipoprotein cholesterol concentration to type of diabetes and its control. Br Med J. 1978, 2: 1191-1194. 10.1136/bmj.2.6146.1191PubMed CentralView ArticlePubMedGoogle Scholar
- Newman-Toker DE, Kerber KA, Hsieh YH, Pula JH, Omron R, Saber Tehrani AS, Mantokoudis G, Hanley DF, Zee DS, Kattah JC: HINTS outperforms ABCD2 to screen for stroke in acute continuous vertigo and dizziness. Acad Emerg Med. 2013, 20: 986-996. 10.1111/acem.12223View ArticlePubMedGoogle Scholar
- Kurth T, Everett BM, Buring JE, Kase CS, Ridker PM, Gaziano JM: Lipid levels and the risk of ischemic stroke in women. Neurology. 2007, 68: 556-562. 10.1212/01.wnl.0000254472.41810.0dPubMed CentralView ArticlePubMedGoogle Scholar
- Uddin MJ, Alam B, Jabbar MA, Mohammad QD, Ahmed S: Association of lipid profile with ischemic stroke. Mymensingh Med J. 2009, 18: 131-135.PubMedGoogle Scholar
- Tohidi M, Mohebi R, Cheraghi L, Hajsheikholeslami F, Aref S, Nouri S, Azizi F, Hadaegh F: Lipid profile components and incident cerebrovascular events versus coronary heart disease; the result of 9 years follow-up in Tehran Lipid and Glucose Study. Clin Biochem. 2013, 46: 716-721. 10.1016/j.clinbiochem.2013.03.012View ArticlePubMedGoogle Scholar
- Koren-Morag N, Tanne D, Graff E, Goldbourt U: Low- and high-density lipoprotein cholesterol and ischemic cerebrovascular disease: the bezafibrate infarction prevention registry. Arch Intern Med. 2002, 162: 993-999. 10.1001/archinte.162.9.993View ArticlePubMedGoogle Scholar
- Lisak M, Demarin V, Trkanjec Z, Basić-Kes V: Hypertriglyceridemia as a possible independent risk factor for stroke. Acta Clin Croat. 2013, 52: 458-463.PubMedGoogle Scholar
- Shahar E, Chambless LE, Rosamond WD, Boland LL, Ballantyne CM, McGovern PG, , Sharrett AR: Plasma lipid profile and incident ischemic stroke: the Atherosclerosis Risk in Communities (ARIC) study. Stroke. 2003, 34: 623-631. 10.1161/01.STR.0000057812.51734.FFView ArticlePubMedGoogle Scholar
- Bowman TS, Sesso HD, Ma J, Kurth T, Kase CS, Stampfer MJ, Gaziano JM: Cholesterol and the risk of ischemic stroke. Stroke. 2003, 34: 2930-2934. 10.1161/01.STR.0000102171.91292.DCView ArticlePubMedGoogle Scholar
- Goldstein LB, Bushnell CD, Adams RJ, Appel LJ, Braun LT, Chaturvedi S, Creager MA, Culebras A, Eckel RH, Hart RG, Hinchey JA, Howard VJ, Jauch EC, Levine SR, Meschia JF, Moore WS, Nixon JV, Pearson TA, American Heart Association Stroke Council; Council on Cardiovascular Nursing; Council on Epidemiology and Prevention; Council for High Blood Pressure Research; Council on Peripheral Vascular Disease, and Interdisciplinary Council on Quality of Care and Outcomes Research: Guidelines for the primary prevention of stroke: a guideline for healthcare professionals from the American Heart Association/American Stroke Association. Stroke. 2011, 42: 517-584. 10.1161/STR.0b013e3181fcb238View ArticlePubMedGoogle Scholar
- Barter PJ: High density lipoprotein: a therapeutic target in type 2 diabetes. Endocrinol Metab. 2013, 28: 169-177. 10.3803/EnM.2013.28.3.169. Seoul,View ArticleGoogle Scholar
- Hayashi T, Kawashima S, Itoh H, Yamada N, Sone H, Watanabe H, Hattori Y, Ohrui T, Yokote K, Nomura H, Umegaki H, Iguchi A, : Low HDL cholesterol is associated with the risk of stroke in elderly diabetic individuals: changes in the risk for atherosclerotic diseases at various ages. Diabetes Care. 2009, 32: 1221-1223. 10.2337/dc08-1677PubMed CentralView ArticlePubMedGoogle Scholar
- Kissela BM, Khoury J, Kleindorfer D, Woo D, Schneider A, Alwell K, Miller R, Ewing I, Moomaw CJ, Szaflarski JP, Gebel J, Shukla R, Broderick JP: Epidemiology of ischemic stroke in patients with diabetes: the greater Cincinnati/Northern Kentucky Stroke Study. Diabetes Care. 2005, 28: 355-359. 10.2337/diacare.28.2.355View ArticlePubMedGoogle Scholar
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