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APOAV (T-1131>C) variant has no effect on mother's height in a large population study
Lipids in Health and Disease volume 3, Article number: 10 (2004)
The important role of APOAV gene T-1131>C variant in determination of plasma triglyceride levels has been proved on many population studies. Recently, associations between C-1131 allele and higher mother's height as well as with longer fetal birth length were suggested.
In 1,305 females, aged between 28 and 67 years and having at least one child, we have analyzed a putative association between T-1131>C APOAV variant (analyzed by PCR and restriction analysis) and body height.
Mother's body height did not differ between T/T homozygotes (N = 1093, 162.5 ± 6.5 cm) and C allele carriers (N = 212, 162.1 ± 6.4 cm). Thus we have failed to confirm, that mothers with APOAV C-1131 allele are higher than T/T-1131 homozygotes.
We read with interest the study by Ward et al.  on the association between the T-1131>C APOAV variant and plasma levels of triglycerides in pregnancy. In agreement with some previously published population's studies with different design [2–8], they described an association between higher levels of plasma triglyceride and the presence of C-1131 allele also in a group of healthy pregnant women. Additionally, they have found an interesting association between allele C-1131 and higher mother's height and as well as with fetal birth length.
The T-1131>C variant has been intensively studied over the last couple of years. The C allele is undoubtedly associated not just with higher plasma TG levels [2–7], but also with higher VLDL-TG levels  what could influence fetal growth [10, 11] and theoretically also final height of the body in adulthood, as suggested by Ward et al. .
Using a previously described method [5, 12], we analyzed the T-1131>C variant in a total of 1,367 unrelated Caucasians females aged 48.8 ± 10.6 years in 2000/2001 recruited as a representative 1% population sample [Multinational monitoring of trends and determinants in cardiovascular diseases: MONICA Project, Manual of operations WHO/MNC 82.2, Nov 1983]. Written informed consent was obtained before the blood samples were taken and measurements being performed. 62 females have no children. In 1,305 females, having at least one child, we have analyzed a putative association between APOAV T-1131>C variant and body height by ANOVA. The size of the analyzed population minimized the chances of false negative or false positive results.
In contrast to Ward et al. , we found no association between the T-1131>C variant in the APOAV gene and mother's body height, see Table 1. Thus the association described by Ward et al.  need not be generally valid.
Plasma triglycerides represent the most important source of fatty acids utilized by fetus through pregnancy. About 50% of these fatty acids are derived from maternal circulation . It has been found that maternal VLDL-TG concentrations are positively correlated with birth weight [10, 11]. In respect of these findings the same effect of VLDL-TG concentrations on birth length could be supposed. However results reveal that birth length seemed to be affected by many maternal factors  shading expected effect of TG.
Nevertheless, adult body height, which slightly correlate with birth length, although undoubtedly genetically determined, will be in last century strongly influenced by abundant availability of food sources in industrially developed countries and this environmental effect could mask the influence of the genes.
More than 1300 unrelated Caucasian females were included in this study. We conclude that the T-1131>C variant in the APOAV gene has no general effect on mother's body height. Nevertheless, APOAV gene and its variants [3, 14] remain to be candidate gene for genetic determination of newborn's length/weight.
Ward KJ, Shields B, Knight B, Salzmann MB, Hattersley AT, Frayling TM: Genetic variants in Apolipoprotein AV alter triglyceride concentrations in pregnancy. Lipids Health Dis. 2003, 2: 9- 10.1186/1476-511X-2-9
Pennacchio LA, Olivier M, Hubacek JA, Cohen JC, Cox DR, Fruchart JC, Krauss RM, Rubin EM: An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing. Science. 2001, 294: 169-73. 10.1126/science.1064852
Pennacchio LA, Olivier M, Hubacek JA, Krauss RM, Rubin EM, Cohen JC: Two independent apolipoprotein AV haplotypes influence human plasma triglyceride levels. Hum Mol Genet. 2002, 11: 3031-8. 10.1093/hmg/11.24.3031
Nabika T, Nasreen S, Kobayashi S, Masuda J: The genetic effect of the apoprotein AV gene on the serum triglyceride level in Japanese. Atherosclerosis. 2002, 165: 201-4. 10.1016/S0021-9150(02)00252-6
Hubacek JA, Skodova Z, Adamkova V, Lanska V, Poledne R: The influence of APOAV polymorphisms (T-1131>C and S19>W) on plasma triglyceride levels and risk of myocardial infarction. Clin Genet. 2004, 65: 126-30. 10.1111/j.0009-9163.2004.00199.x
Hubacek JA, Skodova Z, Adamkova V, Vrablik M, Horinek A, Lanska V, Ceska R, Poledne R: APOAV polymorphisms (T-1131/C and Ser19/Trp) influence plasma triglyceride levels and risk of myocardial infarction. Exp Clin Cardiol. 2004
Talmud PJ, Hawe E, Martin S, Olivier M, Miller GJ, Rubin EM, Pennacchio LA, Humphries SE: Relative contribution of variation within the APOC3/A4/A5 gene cluster in determining plasma triglycerides. Hum Mol Genet. 2002, 11: 3039-46. 10.1093/hmg/11.24.3039
Seda O, Sedova L: New apoliprotein A-V: comparative genomics meets metabolism. Physiol Res. 2003, 52: 141-6.
Talmud PJ, Martin S, Taskinen MR, Frick MH, Nieminen MS, Kesaniemi YA, Pasternack A, Humphries SE, Syvanne M: APOA5 gene variants, lipoprotein particle distribution and progression of coronary heart disease: results from the LOCAT study. J Lipid Res. 2004, 45: 750-756. 10.1194/jlr.M300458-JLR200
Coleman RA: Placental metabolism and transport of lipid. Fed Proc. 1986, 45: 2519-23.
Knopp RH, Bergelin RO, Wahl PW, Walden CE: Relationships of infant birth size to maternal lipoproteins, apoproteins, fuels, hormones, clinical chemistries, and body weight at 36 weeks gestation. Diabetes. 1985, Suppl 2: 71-7.
Horinek A, Vrablik M, Ceska R, Adamkova V, Poledne R, Hubacek JA: T-1131-->C polymorphism within the apolipoprotein AV gene in hypertriglyceridemic individuals. Atherosclerosis. 2003, 167: 369-70. 10.1016/S0021-9150(03)00022-4
Nolan CJ, Riley SF, Sheedy MT, Walstab JE, Beischer NA: Maternal serum triglyceride, glucose tolerance, and neonatal birth weight ratio in pregnancy. Diabetes Care. 1995, 18: 1550-6.
Hubacek JA, Adamková V, Ceska R, Poledne R, Horinek A, Vrablik M: New variants in the apolipoprotein AV gene in individuals with extreme triglyceride levels. Physiol Res. 2004, 53: 225-8.
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Hubacek, J.A., Skodova, Z., Adamkova, V. et al. APOAV (T-1131>C) variant has no effect on mother's height in a large population study. Lipids Health Dis 3, 10 (2004) doi:10.1186/1476-511X-3-10
- Body Height
- Plasma Triglyceride
- Final Height
- Birth Length
- Healthy Pregnant Woman