- Open Access
Serum butyrylcholinesterase in type 2 diabetes mellitus: a biochemical and bioinformatics approach
Lipids in Health and Disease volume 4, Article number: 18 (2005)
Butyrylcholinesterase is an enzyme that may serve as a marker of metabolic syndrome. We (a) measured its level in persons with diabetes mellitus, (b) constructed a family tree of the enzyme using nucleotide sequences downloaded from NCBI. Butyrylcholinesterase was estimated colorimetrically using a commercially available kit (Randox Lab, UK). Phylogenetic trees were constructed by distance method (Fitch and Margoliash method) and by maximum parsimony method.
There was a negative correlation between serum total cholesterol and butyrylcholinesterase (-0.407; p < 0.05) and between serum LDL cholesterol and butyrylcholinesterase (-0.435; p < 0.05). There was no statistically significant correlation among the other biochemical parameters. In the evolutionary tree construction both methods gave similar trees, except for an inversion in the position of Sus scrofa (M62778) and Oryctolagus cuniculus (M62779) between Fitch and Margoliash, and maximum parsimony methods.
The level of butyrylcholinesterase enzyme was inversely related to serum cholesterol; dendrogram showed that the structures from evolutionarily close species were placed near each other.
The enzyme butyrylcholinesterase (BChE; EC 126.96.36.199.8) has a well-defined pharmacologic function in hydrolyzing succinylcholine, a muscle relaxant used in anesthetic practice. It could have other roles, though much less well defined, such as modulating the phenotypic expression of dyslipidemia and metabolic syndrome. Serum levels of the enzyme are affected by dietary fat, obesity, hyperlipidemia and diabetes mellitus .
With genomic sequences from many species being available in the public domain , it is possible to annotate proteins in evolutionarily terms. Such analysis is particularly useful for proteins with poorly defined physiological role, such as BchE. A phylogenetic analysis of amino acid/ nucleotide sequences could throw light on 'how the family might have been derived during evolution' . The analysis is represented as an evolutionary tree, which is a two-dimensional graph; it shows evolutionary relationships of genes from different organisms.
Here we (a) studied the level of BChE among persons with diabetes mellitus (b) constructed the phylogenetic tree of 25 BChE genes from publicly available sequence data.
2. Materials and methods
We studied 30 individuals with clinical type 2 diabetes mellitus at our Centre in southern India (14 men, 16 women; age 51.9 years+/- 7.9 years, duration of diabetes 6.6 years+/-3.74 years). Butyrylcholinesterase was estimated colorimetrically using a commercially available kit (Randox Lab, UK). Serum insulin and C-peptide were measured by radioimmunoassay, and lipids (total cholesterol, triglycerides, HDL cholesterol) by colorimetry.
Results are expressed as mean+/- SD. Multiple correlation analysis was done by SPSS package (v10.5). A p value of < 0.05 was taken as significant.
Twenty five sequences of BchE gene were retrieved from National Centre for Biotechnology Information (NCBI): Rattus norvegicus (Accession no: NM_022942), Mus musculus (NM_009738), homo sapiens (NM_000055), homo sapiens (BC018141), homo sapiens (BC008396), Sus scrofa (AF222914), Gallus gallus (AJ306928), Panthera tigris tigris (AF053484), Felis catus (AF053483), Equus caballus (AF178685), Rattus norvegicus (AF244349), Oryctolagus cuniculus (X52092), Oryctolagus cuniculus (X52091), Oryctolagus cuniculus(X52090), homo sapiens (M16541), Oryctolagus cuniculus(U04814), homo sapiens (M16474), Canis Familiaris (M62411), Bos taurus (M62410), Macaca mulatta (M62777), Ovis aries (M62780), Oryctolagus cuniculus (M62779), Sus scrofa (M62778), Mus musculus (NM_009599), and homo sapiens (NM_000446).
Phylogenetic trees were constructed using two methods: distance method (Fitch and Margoliash method) and maximum parsimony method (MP) .
In the distance method (Fitch and Margoliash) the sequences were aligned by local pair-wise method and a distance score obtained for each pair of sequences (25 × 25 matrix). The scores were represented as a distance matrix; the most closely related sequences in the matrix were identified and represented as a tree/branch. The average distance between these sequences and each of the other sequences was calculated to obtain a new distance matrix. This process was repeated till all sequences were added to the tree.
In the maximum parsimony method sequences were aligned by global pairwise method, possible trees constructed, parsimony cost of each tree calculated and the one with minimum cost identified as the optimal tree, which was the selected output.
The results are presented in Table 1. There was a negative correlation between serum total cholesterol and butyrylcholinesterase (-0.407; p < 0.05) and between serum LDL cholesterol and butyrylcholinesterase (-0.435; p < 0.05). There was no statistically significant correlation among the other biochemical parameters.
Both methods of tree construction gave similar dendrograms, except for an inversion in the position of Sus scrofa (M62778) and Oryctolagus cuniculus (M62779) between Fitch and Margoliash, and maximum parsimony methods (Figs 1, 2). There were two main subdivisions in the phylogenetic tree, with homo sapiens proteins together, and a pairing together of rattus (A5244349, NM_022942), Felicus catus (AF053483), Panthera tigris (AF053485), and Oryctolagus cunniculus (X52092, X53091)
BChE breaks down ester type of neuromuscular blocking agents used in anesthesia . It may also play a role in lipid metabolism , and may be affected by dietary fat . Similarly, diabetes mellitus has been shown to alter the levels of the enzyme. BchE, which can affect cell membrane oxidative stress and fluidity , is structurally homologous to lipase . The ratio of BChE to HDL cholesterol could be a marker of cardiovascular risk in the metabolic syndrome . However recent studies have suggested BchE may not have a direct pathophysiological role in the development of metabolic syndrome , but may be considered as secondary markers for this syndrome in obese individuals with the CHE2 C5- phenotype . In consonance with earlier studies, level of butyrylcholinesterase was related to changes in serum lipids; however there was a relationship to serum triglycerides in a previous study  and to serum cholesterol in ours.
BChE activity could also be used to select the drug for treatment of hypertriglyceridaemia in type 2 diabetes mellitus . Similarly diabetes was one of the risk factors for coronary artery disease that independently correlated with BchE activity .
In the construction of evolutionary trees, the differences in Sus scrofa and Oryctolagus cuniculus was the only variant in the two methods. The sequences being similar, such an output is to be expected. Consistent with an earlier report , the genes of the following were close to each other: cow (Bos taurus M62410) and sheep (Ovis aries M62780), pig (Sus scrofa M62778) and dog (Canis familiaris M62411), rabbit (Oryctolagus cuniculus U04814) and house mouse (Mus musculus NM_009599).
Animal BchE gene structure was shown to be similar to the human gene . The rate of evolution was reported to be rapid, but not more than other proteins with well-known function. BchE evolution had a 2.2 unit evolutionary period (ie, 2.2 million years for a 1% amino acid change) .
A broad based phylogenetic synthesis using combined and separate analysis of published molecular and morphological source phylogenies showed generally comparable tree structures : tiger, cat and horse together.
Despite the limitations in the study, it establishes proof of concept that clinical and biochemical parameters can be linked to data from nucleotide sequences; an evolutionary perspective may be obtained, especially for proteins with poorly defined physiological functions.
In conclusion we (a) found a negative correlation between serum total cholesterol and butyrylcholinesterase and between serum LDL cholesterol in persons with type 2 diabetes (b) generated a phylogenetic tree from 25 sequences of BchE using distance method (Fitch and Margoliash method) and maximum parsimony method.
Sridhar GR, Nirmala G: Inborn errors in lipid metabolism. Lipid disorders. Edited by: Tripathy BB, Das S. 2002, 59-80. Association of Physicians of India, API College of Physicians,
Sridhar GR: Impact of human genome project on medical practice. J Assoc Physicians India. 2001, 49: 905-8.
Mount DW: Phylogenetic prediction. Bioinformatics. Sequence and genome analysis. 2001, 237-80. Cold Spring Harbor Laboratory Press. New York,
Foldes FF: Enzymes of acetylcholine metabolism. Enzymes in Anesthesiology. Edited by: Foldes FF. 1978, 91-168. New York Springer-Verlag,
Krnic Z, Bradamante V: Effects of oxprenolol treatment on pseudocholinesterase and lipids in rats. Arzneimittelforschung. 1997, 47: 910-13.
Kutty KM, Huang SN, Kean KT: Pseudocholinesterase in obesity: hypercaloric diet induced changes in experimental obese mice. Experimentia. 1981, 37: 1141-42. 10.1007/BF01989883
Contrearas JA, Karlsson M, Osterlund T, Laurell H, Svensson A, Holm C: Hormone-sensitive lipase is structurally related to acetylcholinesterase, bile salt-stimulated lipase, and several fungal lipases. Building of a three-dimensional model for the catalytic domain of hormone-sensitive lipase. J Biol Chem. 1996, 271: 31426-30. 10.1074/jbc.271.49.31426
Cucuianu M: Serum gamma glutamyltransferase and/or serum cholinesterase as markers of the metabolic syndrome. Diabetes Care. 1999, 22: 1381-
Randell EW, Mathews MS, Zhang H, Seraj JS, Sun G: Relationship between serum butyrylcholinesterase and the metabolic syndrome. Clin Biochem. 2005, 38: 799-805. 10.1016/j.clinbiochem.2005.04.008
Alcantara VM, Oliveira LC, Rea RR, Suplicy HL, Chautard-Freire-Maia EA: Butyrylcholinesterase activity and metabolic syndrome in obese patients. Clin Chem Lab Med. 2005, 43: 285-8. 10.1515/CCLM.2005.048
Rustemeijer R, Schouten JA, Voerman HJ, Beyenen AC, Donker AJM, Heine RJ: Is pseudocholinesterase activity related to markers of triacylglycerol synthesis in Type II diabetes mellitus?. Clinical Science. 2001, 101: 29-35. 10.1042/CS20000280
Alcantara CM, Chautard-Freire-Maia EA, Scartezini M, Cerci MS, Braun-Prado K, Picheth G: Butyrylcholinesterase activity and risk factors for coronary artery disease. Scand J Clin Lab Invest. 2002, 62: 399-404. 10.1080/00365510260296564
Arpagaus M, Chatonnet A, Masson P, Newton M, Vaughan TA, Bartels CF: Use of polymerase chain reaction for homology probing of butyrylcholinesterase from several vertebrates. J Biol Chem. 1991, 266: 6966-74.
Liu FR, Miyamoto MM, Freire NP, Ong PQ, Tennant MR, Young TS, Gugel KF: Molecular and morphological supertrees for eutherian (placental) mammals. Science. 2001, 291: 1786-9. 10.1126/science.1056346
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
Rights and permissions
Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Sridhar, G., Nirmala, G., Apparao, A. et al. Serum butyrylcholinesterase in type 2 diabetes mellitus: a biochemical and bioinformatics approach. Lipids Health Dis 4, 18 (2005). https://doi.org/10.1186/1476-511X-4-18
- metabolic syndrome
- type 2 diabetes
- phylogenetic tree
- evolutionary distance