Fig. 2

Effect of Cur on intracellular lipid accumulation in Caco-2 cells by activating the TRPA1 channel. A and B Caco-2 cells were incubated with different concentrations (0, 5, 10, 20, 50, and 100 µM) of Cur for 48 h. C and D Cells of the Caco-2 line were incubated with the indicated concentrations of MC (100 µM), Cur (10 µM), and HC (9 µM) for 48 h. A and C Oil red O staining images of Caco-2 cells (magnification 200 ×). B and D Quantification of lipid content by a microplate reader. Data presented as mean ± SEM. (n = 5). ^***P < 0.001 vs control, ^###P < 0.001 vs MC, ^&&P < 0.01 vs MC + Cur. Cur, curcumin; MC, micellar cholesterol; HC, HC-030031; TRPA1, transient receptor potential cation channel subfamily A member 1; SEM, standard error of the mean