Conjugated linoleic acid or omega 3 fatty acids increase mitochondrial biosynthesis and metabolism in skeletal muscle cells
© Vaughan et al.; licensee BioMed Central Ltd. 2012
Received: 20 September 2012
Accepted: 9 October 2012
Published: 30 October 2012
Polyunsaturated fatty acids are popular dietary supplements advertised to contribute to weight loss by increasing fat metabolism in liver, but the effects on overall muscle metabolism are less established. We evaluated the effects of conjugated linoleic acid (CLA) or combination omega 3 on metabolic characteristics in muscle cells.
Human rhabdomyosarcoma cells were treated with either DMSO control, or CLA or combination omega 3 for 24 or 48 hours. RNA was determined using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Mitochondrial content was determined using flow cytometry and immunohistochemistry. Metabolism was quantified by measuring extracellular acidification and oxygen consumption rates.
Omega 3 significantly induced metabolic genes as well as oxidative metabolism (oxygen consumption), glycolytic capacity (extracellular acidification), and metabolic rate compared with control. Both treatments significantly increased mitochondrial content.
Omega 3 fatty acids appear to enhance glycolytic, oxidative, and total metabolism. Moreover, both omega 3 and CLA treatment significantly increase mitochondrial content compared with control.
Polyunsaturated fatty acids (PUFAs) play wide-ranging roles in cell metabolism, signaling and inflammation. Of these PUFAs, very long chain eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) found principally in fish have key roles in metabolism and inflammation [1–18]. EPA has been shown to reduce triacylglyceride formation and improve blood lipid profiles through interactions with sterol-regulatory element binding protein-1c and liver X receptor alpha . DHA has been shown to enhance lipid oxidation and insulin sensitivity in skeletal muscle through AMPK activation . Combinations of omega 3 are commonly consumed, and have been shown to increase fat oxidation, reducing body weight, and prevent weight gain [1, 2, 4–9, 11–15, 17, 18, 20]. Moreover, treatment with combination omega 3 has been shown to triple the expression of genes encoding regulatory factors that control mitochondrial biogenesis and oxidative metabolism including peroxisome proliferator-activated receptor co-activator 1 alpha (PGC-1α) in white adipocytes . Combination omega 3 can now be prescribed to lower triacylglycerides and is currently one of the most common over-the-counter dietary supplements .
Conjugated linoleic acid (CLA), a PUFA found in grass-fed beef among other sources also plays a role in lipid metabolism [18, 22–28]. CLA, like fish oil, is a popular dietary supplement marketed for its role in enhancing fat metabolism. CLA is purported to have several physiological functions, including appetite suppression, increased fat mobilization, and increased fatty acid oxidation [18, 23–25]. Recently, the trans-10,cis-12 CLA but not the cis-9,trans-11 CLA isomer was shown to significantly increase lipolysis in human adipocytes . CLA was also shown to modify hormone sensitive lipase and perilipin expression, key components of fatty acid utilization . Moreover, CLA is purported to reduce fatty acid synthesis in adipocytes, suggesting that CLA discourages fat deposition directly contributing to body composition [22, 24]. Interestingly, rodents were shown to be resistant to diet-induced weight gain following treatment with CLA, and had increased lipid oxidation with reduced levels of plasma insulin . Rodent models have also shown significant weight loss when treated with CLA [24, 27]. In addition, treatment of rodents with CLA reduces weight as well as increases hepatic RNA expression associated with fatty acid oxidation .
Clinically, mitochondrial dysfunction is associated with reduced capacity for fatty acid oxidation and inversely related to incidence of type II diabetes and obesity [29–34]. PGC-1α, an essential stimulator of mitochondrial biosynthesis has been shown to increase fatty acid oxidation through induction of peroxisome proliferator-activated receptor alpha (PPARα) [35–40]. PGC-1α expression is inversely related to incidence of type II diabetes and obesity and reduced propensity for fatty acid oxidation [29–34]. Induction of PGC-1α has also been shown to heighten metabolic rate through increased expression of mitochondrial uncoupling proteins [33, 39–41]. Irisin, a hormone released by skeletal muscle following exercise, is induced by PGC-1α expression and increases metabolic rate through uncoupling protein 1 induction .
The role of PUFAs such as omega 3 and CLA in glucose metabolism and cellular uptake is less understood. Induction of PGC-1α has been linked to increased glucose transport and insulin sensitivity through glucose transporter 4 (GLUT4) . GLUT4 is an insulin dependent glucose transporter found almost exclusively in skeletal muscle and adipocytes. An increase in GLUT4 expression is evidence of increased glucose uptake and glycolytic reliance .
While there is evidence supporting a role for PUFAs in lipid metabolism in hepatocytes and adipocytes, there is limited evidence evaluating the effects of omega 3 fatty acids and CLA on human skeletal muscle cell metabolism. Because muscle cell metabolism can also play a significant role in body composition, we investigated the effects of a combination of docosahexaenoic acid and eicosapentaenoic acid (combination omega 3) and CLA on oxidative and glycolytic capacities and related gene expression, as well as mitochondrial biosynthesis in human muscle cells.
Combination omega 3 significantly increased glycolytic capacity in muscle cells compared with control without suppressing oxidative metabolism suggesting that omega 3 increased total metabolisms (Figure 1B and 2B). Combination omega 3 significantly raised baseline oxygen consumption, a measure of oxidative metabolism and fatty acid oxidation as previously demonstrated [2, 11, 15–17, 21]. Combination omega 3 also significantly decreased the ratio of OCR:ECAR suggesting that omega 3 fatty acids not only increase glycolytic capacity but also increase total glycolytic reliance. Treatment with CLA at 25 μM significantly decreased both glycolytic and oxidative metabolism. A decreased OCR:ECAR ratio suggests that, although total metabolism is suppressed, this treatment also induces a shift towards glycolytic metabolism. CLA at 50 μM did not alter ECAR, however OCR was reduced, also resulting in a lower OCR:ECAR ratio indicating a shift towards glycolytic metabolism.
The finding that maximum oxygen consumption is not increased in any treatment is interesting in light of the findings of increased mitochondrial content. First, following treatment for 24 and 48 hours, omega 3 significantly induced PGC-1α, an essential precursor for mitochondrial biosynthesis. This finding is supported by the increase in total mitochondrial content observed by both flow cytometry and microscopy. This suggests that both treatments are effective at increasing mitochondrial number, density and networking without influencing mitochondrial activity. Omega 3 treatment for 24 and 48 hours also significantly induced Irisin, a down-stream target of PGC-1α shown to enhance metabolic rate in rodents following exercise . Moreover, omega 3 also increased GLUT4 expression, an insulin dependent glucose transporter exclusive to muscle cells and adipocytes, supporting the observation of increased glycolytic capacity.
The timing and duration of treatment played a significant role on mitochondrial changes. Treatment for 48 hours with repeated treatment at 24 hours also caused significantly greater mitochondrial staining compared with control. Remarkably, a single treatment for 48 hours has no significant effect on mitochondrial staining and a limited effect on gene expression; only Irisin expression was significantly greater than control following a single treatment for 48 hours. This observation supports the notion that while fish oils have many documented powerful effects, regular treatment may be necessary to sustain the potentially beneficial properties [2, 11, 15–17, 21].
Fish oil supplements and other polyunsaturated fatty acids including CLA are marketed heavily for their effects on metabolism. This work identified several effects that omega 3 fatty acids EPA and DHA as well as CLA (available over the counter to consumers) have on metabolism and mitochondrial characteristics in human muscle cells. Combination omega 3 and CLA increased the ratio of glycolytic metabolism to oxidative metabolism. However, with CLA treatment, the ratio is altered because of a decrease in oxidative metabolism rather than an increase in glycolytic metabolism, suggesting lower overall metabolism. We hypothesize that the clinical metabolic benefits of CLA consumption are due to the CLA-induced apoptosis of adipocytes in mammals, which liberates and increases fatty acid oxidation elsewhere in the body [45, 46]. Omega 3 treatment significantly increased basal oxidative metabolism as well as basal and peak glycolytic metabolism. Because glycolytic metabolism is much less efficient, this shift likely results in greater glucose uptake. This is supported by up-regulation of the GLUT4 transporter. Based on these studies, combination omega 3 appears to be a potent stimulator of metabolism in muscle cells. More work is needed to identify the full capabilities of these bioactive lipids and the many other effects they likely have on metabolism.
Materials and methods
Cell culture and treatments
Homo sapiens rhabdomyosarcoma cells were purchased from ATCC (Manassas, VA). Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 4500mg/L glucose and supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 100U/mL penicillin/streptomycin, in a humidified 5% CO2 atmosphere at 37°C. Trypsin-EDTA at 0.25% was used to detach the cells for splitting and re-culturing. All reagents were from Sigma (St. Louis, MO). Stock combination DHA:EPA with ratio of 1:2.5 (combination omega 3) or CLA from General Nutrition Center (Pittsburg, PA) was dissolved in DMSO to make treatment solutions; final concentration of DMSO 0.1% for all treatments. Cells were treated with either 25 μM or 50 μM omega 3 or 25 μM or 50 μM CLA and incubated for 24 or 48 hours (determined through pilot experiments) as described above.
Quantitative real time polymerase chain reaction (qRT-PCR)
Forward and reverse primer sequences used for qRT-PCR measurements synthesized by Integrated DNA Technologies (Coralville, IA)
Forward primer 5′→ 3′
Reverse primer 5′→ 3′
Cells were plated into 6-well plates at a density of 1.2 × 106 cells/well treated in triplicate and incubated as previously described for 24 or 48 hours. The cells were pelleted, the media was removed and the cells were suspended in pre-warmed media with 200 nM Mitotracker Green from Life Technologies (Carlsbad, CA) and incubated for 45 minutes (per manufactures’ protocol) and were incubated as previously described. The cells were pelleted, the media with Mitotracker was removed and the cells were suspended in pre-warmed media. Group mean fluorescence was measured using Facscalibur filtering at 488nm.
Chamber-slides from BD Bioscience (Sparks, MD), were seeded with 5000 cells/well and treated in triplicate and incubated as previously described for 24 hours. The cells were then stained with either Mitotracker from Invitrogen (Grand Island, NY) for 45 minutes, and fixed in 3.7% formaldehyde in pre-warmed media. Cells were mounted with Prolong Gold with DAPI from Invitrogen (Carlsbad, CA) and cured overnight. Cells were imaged using the Axiovert 25 microscope with AxioCam MRc from Zeiss (Thornwood, NY).
Cells were seeded overnight in 24-well culture plate from SeaHorse Bioscience (Billerica, MA) at density 5 × 105 cells/well. Cells were treated and incubated for 24 hours as described above. Following treatment, culture media was removed and replaced with XF Assay Media from SeaHorse Bioscience (Billerica, MA) containing 4500mg/L glucose free of CO2 and incubated at 37°C. Per manufactures’ protocol, SeaHorse injection ports were loaded with oligomycin, and inhibitor of oxidative metabolism and maximizes glycolytic metabolism (final concentration 1.0 μM), carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP), an uncoupler of electron transport maximizes oxidative metabolism (final concentration 1.25 μM), and rotenone in 1.0 μM final concentration. Extracellular acidification, a measure of glycolytic capacity, and oxygen consumption, a measure of oxidative metabolism was measured using the SeaHorse XF24 Extracellular Analyzer from SeaHorse Bioscience (Billerica, MA). SeaHorse XF24 Extracellular Analyzer was run using 8 minute cyclic protocol commands (mix for 3 minutes, let stand 2 minutes, and measure for 3 minutes) in triplicate.
Cells were seeded in 96-well plates at density 5,000 cells/well and grown over night. Cells were treated and incubated as previously described for 24 or 48 hours. Media and treatment were removed at each time point and media containing 10% WST1 assay was added to each well and were incubated as previously described. Fluorescence was measured 1 hour following WST1 addition using Wallac Victor3V 1420 Multilabel Counter from PerkinElmer (Waltham, MA).
RNA gene expression, WST1 assay, cell metabolism, and flow cytometry were analyzed using ANOVA and pairwise comparisons comparing treatments with control. Values of p < 0.05 indicated statistical significance in all tests used and Bonferroni’s correction for error from multiple pairwise comparisons was used.
This work was supported in part by the University of New Mexico Summer 2012 Office of Graduate Studies Research, Project and Travel Grant. We would also like to acknowledge the University of New Mexico Department of Biochemistry and Molecular Biology for their assistance in this work. The authors and contributors of this work report no conflict of interest.
- Arai T, Kim HJ, Chiba H, Matsumoto A: Anti-Obesity Effect of Fish Oil and Fish Oil-Fenofibrate Combination in Female KK Mice. J Atheroscler Thromb. 2009, 16: 674-683.View ArticlePubMedGoogle Scholar
- Banga A, Unal R, Tripathi P, Pokrovskaya I, Owens RJ, Kern PA, Ranganathan G: Adiponectin translation is increased by the PPAR gamma agonists pioglitazone and omega-3 fatty acids. Am J Physiol Endocrinol Metab. 2009, 296: E480-E489.PubMed CentralView ArticlePubMedGoogle Scholar
- Calder PC: Omega-3 Fatty Acids and Inflammatory Processes. Nutrients. 2010, 2: 355-374.PubMed CentralView ArticlePubMedGoogle Scholar
- Calder PC: Fatty acids and inflammation: The cutting edge between food and pharma. Eur J Pharmacol. 2011, 668: S50-S58.View ArticlePubMedGoogle Scholar
- Duda MK, Xu WH, Tintinu A, O'Shea KM, Stanley WC: Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) Supplementation, but not alpha-Linolenic Acid, Elevates Plasma Adiponectin Concentration and Prevents Pressure Overload Induced Ventricular Dysfunction and Remodeling. Circulation. 2008, 118: S542-S542.Google Scholar
- Fedor D, Kelley DS: Prevention off insulin resistance by n-3 polyunsaturated fatty acids. Curr Opin Clin Nutr Metab Care. 2009, 12: 138-146.View ArticlePubMedGoogle Scholar
- Flachs P, Horakova O, Brauner P, Rossmeisl M, Pecina P, Franssen-van Hal N, Ruzickova J, Sponarova J, Drahota Z, Vlcek C: Polyunsaturated fatty acids of marine origin upregulate mitochondrial biogenesis and induce beta-oxidation in white fat. Diabetologia. 2005, 48: 2365-2375.View ArticlePubMedGoogle Scholar
- Flachs P, Mohamed-Ali V, Horakova O, Rossmeisl M, Hosseinzadeh-Attar MJ, Hensler M, Ruzickova J, Kopecky J: Polyunsaturated fatty acids of marine origin induce adiponectin in mice fed a high-fat diet. Diabetologia. 2006, 49: 394-397.View ArticlePubMedGoogle Scholar
- Flachs P, Rossmeisl M, Bryhn M, Kopecky J: Cellular and molecular effects of n-3 polyunsaturated fatty acids on adipose tissue biology and metabolism. Clin Sci. 2009, 116: 1-16.View ArticlePubMedGoogle Scholar
- Hassanali Z, Ametaj BN, Field CJ, Proctor SD, Vine DF: Dietary supplementation of n-3 PUFA reduces weight gain and improves postprandial lipaemia and the associated inflammatory response in the obese JCR:LA-cp rat. Diabetes Obesity & Metabolism. 2010, 12: 139-147.View ArticleGoogle Scholar
- Hull MA: Omega-3 polyunsaturated fatty acids. Best Practice & Research in Clinical Gastroenterology. 2011, 25: 547-554.View ArticleGoogle Scholar
- Kopecky J, Rossmeisl M, Flachs P, Kuda O, Brauner P, Jilkova Z, Stankova B, Tvrzicka E, Bryhn M: n-3 PUFA: bioavailability and modulation of adipose tissue function. Proc Nutr Soc. 2009, 68: 361-369.View ArticlePubMedGoogle Scholar
- Krebs JD, Browning LM, McLean NK, Rothwell JL, Mishra GD, Moore CS, Jebb SA: Additive benefits of long-chain n-3 polyunsaturated fatty acids and weight-loss in the management of cardiovascular disease risk in overweight hyperinsulinaemic women. Int J Obes. 2006, 30: 1535-1544.View ArticleGoogle Scholar
- Lam YY, Hatzinikolas G, Weir JM, Janovska A, McAinch AJ, Game P, Meikle PJ, Wittert GA: Insulin-stimulated glucose uptake and pathways regulating energy metabolism in skeletal muscle cells: The effects of subcutaneous and visceral fat, and long-chain saturated, n-3 and n-6 polyunsaturated fatty acids. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2011, 1811: 468-475.View ArticleGoogle Scholar
- Micallef M, Munro I, Phang M, Garg M: Plasma n-3 polyunsaturated fatty acids are negatively associated with obesity. Br J Nutr. 2009, 102: 1370-1374.View ArticlePubMedGoogle Scholar
- Ramel A, Parra D, Alfredo Martinez J, Kiely M, Thorsdottir I: Effects of seafood consumption and weight loss on fasting leptin and ghrelin concentrations in overweight and obese European young adults. Eur J Nutr. 2009, 48: 107-114.View ArticlePubMedGoogle Scholar
- Thorsdottir I, Tomasson H, Gunnarsdottir I, Gisladottir E, Kiely M, Parra MD, Bandarra NM, Schaafsma G, Martinez JA: Randomized trial of weight-loss-diets for young adults varying in fish and fish oil content. Int J Obes. 2007, 31: 1560-1566.View ArticleGoogle Scholar
- Vemuri M, Kelley DS, Mackey BE, Rasooly R, Bartolini G: Docosahexaenoic acid (DHA) but not eicosapentaenoic acid (EPA) prevents trans-10, cis-12 conjugated linoleic acid (CLA) - Induced insulin resistance in mice. Metab Syndr Relat Disord. 2007, 5: 315-322.View ArticlePubMedGoogle Scholar
- Zaima N, Sugawara T, Goto D, Hirata T: Trans geometric isomers of EPA decrease LXR alpha-induced cellular triacylglycerol via suppression of SREBP-1c and PGC-1 beta. J Lipid Res. 2006, 47: 2712-2717.View ArticlePubMedGoogle Scholar
- Gunnarsdottir I, Tomasson H, Kiely M, Martinez JA, Bandarra NM, Morais MG, Thorsdottir I: Inclusion of fish or fish oil in weight-loss diets for young adults: effects on blood lipids. Int J Obes. 2008, 32: 1105-1112.View ArticleGoogle Scholar
- Bhatnagar D, Hussain F: Omega-3 fatty acid ethyl esters (Omacor (R)) for the treatment of hypertriglyceridemia. Futur Lipidol. 2007, 2: 263-270.View ArticleGoogle Scholar
- Choi YJ, Kim YC, Han YB, Park Y, Pariza MW, Ntambi JM: The trans-10, cis-12 isomer of conjugated linoleic acid downregulates stearoyl-CoA desaturase 1 gene expression in 3T3–L1 adipocytes. J Nutr. 2000, 130: 1920-1924.PubMedGoogle Scholar
- Chung SY, Brown JM, Sandberg MB, McIntosh M: Trans-10, cis-12 CLA increases adipocyte lipolysis and alters lipid droplet-associated proteins: role of mTOR and ERK signaling. J Lipid Res. 2005, 46: 885-895.PubMed CentralView ArticlePubMedGoogle Scholar
- Larsen TM, Toubro S, Astrup A: Efficacy and safety of dietary supplements containing CLA for the treatment of obesity: evidence from animal and human studies. J Lipid Res. 2003, 44: 2234-2241.View ArticlePubMedGoogle Scholar
- Li JJ, Huang CJ, Xie D: Anti-obesity effects of conjugated linoleic acid, docosahexaenoic acid, and eicosapentaenoic acid. Mol Nutr Food Res. 2008, 52: 631-645.View ArticlePubMedGoogle Scholar
- Takahashi Y, Kushiro M, Shinohara K, Ide T: Activity and mRNA levels of enzymes involved in hepatic fatty acid synthesis and oxidation in mice fed conjugated linoleic acid. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. 2003, 1631: 265-273.View ArticleGoogle Scholar
- West DB, Delany JP, Camet PM, Blohm F, Truett AA, Scimeca J: Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am J Physiol Regul Integr Comp Physiol. 1998, 275: R667-R672.Google Scholar
- Yanagita T, Wang YM, Nagao K, Ujino Y, Inoue N: Conjugated linoleic acid-induced fatty liver can be attenuated by combination with docosahexaenoic acid in C57BL/6N mice. J Agric Food Chem. 2005, 53: 9629-9633.View ArticlePubMedGoogle Scholar
- Patti ME, Butte AJ, Crunkhorn S, Cusi K, Berria R, Kashyap S, Miyazaki Y, Kohane I, Costello M, Saccone R: Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: Potential role of PGC1 and NRF1. Proc Natl Acad Sci U S A. 2003, 100: 8466-8471.PubMed CentralView ArticlePubMedGoogle Scholar
- Semple RK, Crowley VC, Sewter CP, Laudes M, Christodoulides C, Considine RV, Vidal-Puig A, O'Rahilly S: Expression of the thermogenic nuclear hormone receptor coactivator PGC-1 alpha is reduced in the adipose tissue of morbidly obese subjects. Int J Obes. 2004, 28: 176-179.View ArticleGoogle Scholar
- Yang XL, Enerback S, Smith U: Reduced expression of FOXC2 and brown adipogenic genes in human subjects with insulin resistance. Obes Res. 2003, 11: 1182-1191.View ArticlePubMedGoogle Scholar
- Chowdhury SKR, Dobrowsky RT, Femyhough P: Nutrient excess and altered mitochondrial proteome and function contribute to neurodegeneration in diabetes. Mitochondrion. 2011, 11: 845-854.View ArticlePubMedGoogle Scholar
- Mootha VK, Handschin C, Arlow D, Xie XH, St Pierre J, Sihag S, Yang WL, Altshuler D, Puigserver P, Patterson N: Err alpha and Gabpa/b specify PGC-1 alpha-dependent oxidative phosphorylation gene expression that is altered in diabetic muscle (vol 101, pg 6570, 2004). Proc Natl Acad Sci U S A. 2005, 102: 10405-10405.Google Scholar
- Schreiber SN, Emter R, Hock MB, Knutti D, Cardenas J, Podvinec M, Oakeley EJ, Kralli A: The estrogen-related receptor alpha (ERR alpha) functions in PPAR gamma coactivator 1 alpha (PGC-1 alpha)-induced mitochondrial biogenesis. Proc Natl Acad Sci U S A. 2004, 101: 6472-6477.PubMed CentralView ArticlePubMedGoogle Scholar
- Yoon JC, Puigserver P, Chen GX, Donovan J, Wu ZD, Rhee J, Adelmant G, Stafford J, Kahn CR, Granner DK: Control of hepatic gluconeogenesis through the transcriptional coactivator PGC-1. Nature. 2001, 413: 131-138.View ArticlePubMedGoogle Scholar
- Puigserver P, Rhee J, Donovan J, Walkey CJ, Yoon JC, Oriente F, Kitamura Y, Altomonte J, Dong HJ, Accili D, Spiegelman BM: Insulin-regulated hepatic gluconeogenesis through FOXO1-PGC-1 alpha interaction. Nature. 2003, 423: 550-555.View ArticlePubMedGoogle Scholar
- Vega RB, Huss JM, Kelly DP: The coactivator PGC-1 cooperates with peroxisome proliferator-activated receptor alpha in transcriptional control of nuclear genes encoding mitochondrial fatty acid oxidation enzymes. Mol Cell Biol. 2000, 20: 1868-1876.PubMed CentralView ArticlePubMedGoogle Scholar
- Wu H, Kanatous SB, Thurmond FA, Gallardo T, Isotani E, Bassel-Duby R, Williams RS: Regulation of mitochondrial biogenesis in skeletal muscle by CaMK. Science. 2002, 296: 349-352.View ArticlePubMedGoogle Scholar
- Knutti D, Kaul A, Kralli A: A tissue-specific coactivator of steroid receptors, identified in a functional genetic screen. Mol Cell Biol. 2000, 20: 2411-2422.PubMed CentralView ArticlePubMedGoogle Scholar
- Esterbauer H, Oberkofler H, Krempler F, Patsch W: Human peroxisome proliferator activated receptor gamma coactivator 1 (PPARGC1) gene: cDNA sequence, genomic organization, chromosomal localization, and tissue expression. Genomics. 1999, 62: 98-102.View ArticlePubMedGoogle Scholar
- Wu ZD, Puigserver P, Andersson U, Zhang CY, Adelmant G, Mootha V, Troy A, Cinti S, Lowell B, Scarpulla RC, Spiegelman BM: Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1. Cell. 1999, 98: 115-124.View ArticlePubMedGoogle Scholar
- Bostroem P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Bostroem EA, Choi JH, Long JZ: A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012, 481: 463-U472.View ArticleGoogle Scholar
- Philp A, Belew MY, Evans A, Pham D, Sivia I, Chen A, Schenk S, Baar K: The PGC-1 alpha-related coactivator promotes mitochondrial and myogenic adaptations in C2C12 myotubes. Am J Physiol Regul Integr Comp Physiol. 2011, 301: R864-R872.PubMed CentralView ArticlePubMedGoogle Scholar
- Armoni M, Quon MJ, Maor G, Avigad S, Shapiro DN, Harel C, Esposito D, Goshen Y, Yaniv I, Karnieli E: PAX3/Forkhead homolog in rhabdomyosarcoma oncoprotein activates glucose transporter 4 gene expression in vivo and in vitro. J Clin Endocrinol Metab. 2002, 87: 5312-5324.View ArticlePubMedGoogle Scholar
- Zhai J-j, Liu Z-l, Li J-m, Chen J-P, Jiang L, Wang D-m, Yuan J, Shen J-G, Yang D-P, Chen J-Q: Different mechanisms of cis-9, trans-11-and trans-10, cis-12-conjugated linoleic acid affecting lipid metabolism in 3T3-L1 cells. J Nutr Biochem. 2010, 21: 1099-1105.View ArticlePubMedGoogle Scholar
- Rayalam S, Della-Fera MA, Baile CA: Phytochemicals and regulation of the adipocyte life cycle. J Nutr Biochem. 2008, 19: 717-726.View ArticlePubMedGoogle Scholar
- Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29: 2002-2007.View ArticleGoogle Scholar
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