- Open Access
A single-nucleotide polymorphism in the proximal promoter region of the apolipoprotein M gene is associated with dyslipidaemia but not increased coronary artery diseases in Chinese populations
© Cao et al.; licensee BioMed Central Ltd. 2013
- Received: 5 October 2013
- Accepted: 11 December 2013
- Published: 16 December 2013
It has been reported that rs940494 and rs805296 SNPs of apolipoprotein M (apoM) gene may confer the risk in the development of type 2 diabetes (T2D) and coronary artery disease (CAD) in the Han Chinese. However, a recent study demonstrated that rs805297 polymorphism is significantly associated with reduced total high density lipoprotein (HDL) levels in rheumatoid arthritis patients. But the relationship between rs805297 SNP and CAD has not been explored. The aim of the present study was to elucidate whether the rs805297 mutant allele is implicated in CAD and links to changes in blood lipid levels in these patients.
Three hundred CAD patients and three hundred and twelve non-CAD patients were subjected in the present study. All subjects were confirmed by the angiography. Plasma concentrations of apoM were semi-quantitatively determined by dot-blotting analysis, and total serum lipid levels were quantified using an automated RA-1000 (Technician, USA). The genotyping of rs805297 of apoM was analyzed by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP).
Genotype and allele frequencies were not significant (P = 0.5798 and 0.3834, respectively) between cases and controls. Compared with the wild-type C/C genotype, carriers of the C/A and A/A genotypes did not have an increased risk of CAD, as determined by multiple logistic regression analysis, after adjustment for age, sex, BMI, history of smoking, hypertension and hypercholesterolemia. (CA, odds ratio = 0.49, 95% confidence interval 0.15–1.87, P = 0.462; AA, odds ratio = 0.51, 95% confidence interval 0.13–1.68, P = 0.534). The plasma concentration levels of apoM did not differ significantly among carriers of the three genotypes between two groups. Lastly, control subjects with A/A genotypes had lower total levels of HDL cholesterol than did those with C/C genotypes.
The results presented here suggest that the rs805297 SNP is not associated with an increased risk of developing CAD, although it does independently correlate with dyslipidaemia in Han Chinese individuals.
- Coronary artery disease
- Genetic polymorphism
- Risk factor
Coronary artery disease (CAD) is one of the most common causes of death globally, including in China. CAD is a complex multi-factorial and polygenic disorder that is thought to result from the interactions of an individual's genetic background and several environmental factors[1, 2].
Apolipoprotein M (apoM) is predominantly present in HDL, and to a lesser extent in chylomicrons, very low density lipoprotein (VLDL) and low-density lipoprotein (LDL). It plays an important role in reverse cholesterol transport. The apoM gene is proposed to play a protective role against the development of CAD. Recent studies suggest that several single nucleotide polymorphisms (SNPs), including rs805297, rs940494 and rs805296 occur in the Han Chinese population. Rs940494 and rs805296 have been identified to associate with Type 2 diabetes or CAD[6–8]. Rs805297 SNP is significantly associated with reduced total HDL levels in rheumatoid arthritis patients. It is well known that reduced high-density lipoprotein (HDL) cholesterol levels is one of risk factors of CAD, but the relationship between rs805297 and CAD has not been explored.
The objective of the present study was to investigate whether there is an association between a common SNP in the apoM gene, namely the C-1065A polymorphism, and the risk of CAD in a Chinese population. We chose to evaluate this SNP in particular because it is located in the apoM promoter region, and changes in apoM expression levels may increase the risk of CAD or other complex diseases where lipid levels are pathologically altered.
Characteristics of the total study population
CAD patients (n = 300)
Control subjects (n =312)
Age ( years )
62.3 ± 10.1
60.8 ± 11.2
Sex ( M / F )
Habitual smoking (%)
BMI ( kg / m 2 )
25.1 ± 3.09
23.9 ± 3.2
TC ( mmol / L )
4.51 ± 1.31
4.47 ± 1.25
TG ( mmol / L )
1.84 ± 1.02
HDL - C ( mmol / L )
1.25 ± 0.42
LDL - C ( mmol / L )
2.27 ± 0.97*
2.39 ± 0.78
Distribution of genotypes between CAD patients and control subjects
CAD patients (n = 300)
Control subjects (n = 312)
Logistic regression analysis for the association between apoM genotypes and the risk of coronary artery disease
Lipid profile values (mmol/L) depending on apoM genotypes
CAD patients (n = 300)
Control subjects (n = 312)
4.52 ± 1.21
4.48 ± 1.45
4.46 ± 1.31
4.48 ± 1.27
4.45 ± 1.19
4.43 ± 1.06
1.93 ± 1.12
1.81 ± 1.05
1.88 ± 0.97
1.09 ± 0.23
1.11 ± 0.38
1.05 ± 0.35
1.27 ± 0.42
1.24 ± 0.37
1.07 ± 0.39*
2.25 ± 1.07
2.28 ± 1.13
2.31 ± 0.98
2.39 ± 0.75
2.38 ± 0.83
2.33 ± 0.68
The pathophysiology of atherosclerosis is multifactorial with environmental, metabolic and genetic components influencing etiology. A large body of literature has established a panel of traditional candidate genes implicated in cardiovascular disease, such as angiotensin-converting enzyme (ACE) and angiotensinogen (AGT). Variants in these traditional genes are known to be correlated with coronary artery disease (CAD). Recent studies have identified new loci to the growing list of the candidate genes, including myocyte enhancer factor 2 (MEF2A) and phosphodiesterase 4 (PDE4D). ApoM is a novel potential candidate gene that may be protective against the development of CAD.
In present case–control study, we sought to determine whether rs805297 SNP of the apoM gene alters susceptibility to CAD. First, we compared genotype distributions and allele frequencies of the apoM gene between CAD and control groups and then performed a logistic regression with adjustments for age, sex, BMI, history of smoking, hypertension and hypercholesterolemia. Our data identified no relationship between CAD and the apoM genotype, supporting that rs805297 SNP is not a risk factor for genetic susceptibility to CAD.
Previous studies have reported associations between rs805297 and rs940494 SNPs of apoM with CAD in Chinese populations[7, 8]. Contrarily, our study did not detect any differences in genotype distributions and allele frequencies of rs805297 SNP of apoM gene between CAD and control groups in our Han Chinese subjects. This may be attributed to different allele frequencies of apoM rs805297 in our study compared to previous studies, which may be due to differences in age, sex, race, geographic location or population sample size. Our study is limited by it’s case–control design; future prospective studies that include haplotype analysis may overcome some of these limitations.
It is thought that apoM plays a role in lipid metabolism; however, the biological mechanisms of this protein are not fully understood. Previous studies have identified associations between apoM and plasma lipoproteins, demonstrating correlations with size, charge and composition. Plasma apoM is strongly correlated with total cholesterol in healthy individuals, with apoM concentrations roughly corresponding to 1/50th of the apoA-I plasma concentration. This prompted us to investigate the impact of the C-1065A polymorphism on the plasma lipid profile. Consistent with a recent report, our study found no significant differences between rs805297 SNP and atherogenic lipids (TC, TG, LDL-C). We found no significant differences in HDL between CAD patients with A/A genotypes and those with C/C or C/A genotypes, although control subjects with A/A genotypes had lower levels of HDL than those with C/C or A/A genotypes. It is possible that statin treatments for hyperlipidemia in CAD patients modulated the lipid parameters.
The apoM gene is located in histocompatibility complex III (HMC-III) region on chromosome 6. The rs805297 SNP site is localized to the promoter, which may affect gene transcription and concomitant protein levels in the blood to underlie its disease association. This hypothesis is supported by previous literature indicating that the C allele of rs805296 SNP may increase promoter activity and confer the risk susceptibility to type I diabetes. Additionally, Hae-Jin Hu and colleagues suggested that rs805297 polymorphism in the apoM promoter may decrease its transcriptional activity and thus alter HDL-C concentrations to ultimately affect RA susceptibility. In light of these previous findings, we evaluated the impact of rs805297 polymorphism on plasma concentration of apoM. Based on non-significant differences in plasma apoM concentrations, our data suggests that the mutant promoter does not affect apoM gene expression. It is possible that this polymorphism is functionally silent which many explain our negative finding. In the present study, we used a semi-quantitative method to estimate serum apoM levels; therefore, future studies should apply more precise measures to determine whether this finding is replicable.
The data in this present study support that the rs805297 SNP is not associated with increased susceptibility to CAD. Our data indicates that this polymorphism is associated with dyslipidemia in healthy controls in the Han Chinese populations.
Subjects recruited for this study consisted of 300 CAD patients (194 males and 106 females with a mean age of 62.3 ± 10.1 years) and 312 unrelated individuals enrolled as control subjects (183 males and 129 females with a mean age of 60.8 ± 11.2 years). To be enrolled in the CAD group, patients, had to have at least 50% stenosis, determined angiographically, in at least one of the major segments of coronary arteries, i.e., the right coronary artery, left circumflex, or left anterior descending arteries. CAD patients suffering from acute coronary syndrome or undergoing previous cardiovascular events such as angioplasty or CABG or stenting were not enrolled in CAD group. Controls were required to have a negative coronary artery angiography so as to exclude CAD. History of conventional risk factors for CAD, including habitual cigarette smoking (persons with smoking habit smoke more than 1 cigarette per day averagely), hypertension (systolic blood pressure ≥160 mm Hg and/or diastolic blood pressure ≥95 mmHg), diabetes mellitus (A diagnosis of diabetes is confirmed if there are symptoms of diabetes and a plasma glucose level of at least 11.1 mmol/L, a fasting plasma glucose level of at least 7 mmol/L; or a two-hour plasma glucose level of at least 11.1 mmol/L during an oral glucose tolerance test.), or hypercholesterolemia (TC ≥ 5.7 mmol/L) were obtained from the subjects or from their medical records. Exclusion criteria for both the CAD patients and control individuals included familial hypercholesterolemia, diabetes mellitus, cancer, renal disease, and any other chronic illnesses. All subjects in both the CAD and control groups are Han Chinese from Jiangsu province or Shanghai, China. The research was in agreement with Helsinki declaration. The research was prospectively reviewed and approved by a duly constituted ethics committee at Nanjing Medical University. Written informed consent was obtained from each subject before collecting blood samples. A medical history and a peripheral venous blood specimen were obtained from all participants; part of this blood sample was analyzed for plasma levels of fasting glucose, triglycerides (TG), total cholesterol (TC), HDL–cholesterol (HDL-C) and LDL–cholesterol (LDL-C), all of which were measured using an automated RA-1000 (Technician, USA).
Genotyping of the human apoM gene
Five milliliters (5 ml) of venous blood was collected from each subject into 50 mmol/L disodium EDTA as an anticoagulant, and genomic DNA was extracted from peripheral blood leukocytes, as described previously. The apoM polymorphism was analyzed by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). DNA was amplified using the following primers: forward primer, 5′-GCTTTGCAAACATTACTATTCAT-3′, reverse primer, 5′-ATTGGCAAATCATCAATCTTATA-3′.6 Amplification reactions were performed in a total volume of 50 μl containing: 1 μg of genomic DNA, 12.5 pmol of each primer, 2.5 mmol/L each of dCTP, dTTP, dGTP, and dATP, 2 U of Taq DNA polymerase (Promega, USA), 50 mmol/L KCl, 1.5 mmol/L MgCl2. 0.1% Triton X-100 and 10 mmol/L Tris–HCl (pH 9.0 at 25°C). The thermocycling procedure consisted of an initial denaturation at 94°C for 5 min; 35 cycles of denaturation (94°C for 30 s), annealing (48°C for 30 s), and extension (72°C for 30 s); and a final extension at 72°C for 5 min. PCR products were first subject to restriction digest by Tsp509I for 16 h at 65°C; the reaction was performed in a total volume of 20 μl containing 1 μg of PCR product; 5 U of restriction endonuclease Tsp509I (New England Biolabs, USA) and 2 μl of restriction enzyme buffer. Genotypes were then used to detect the presence of apoM polymorphisms on a 4% low molecular weight agarose gel electrophoresis system (Promega, USA) and visualized by ethidium bromide staining. The Cys→Ala substitution creates a Tsp509I restriction site in the rs805297 polymorphism of apoM. PCR products with an Ala allele were digested into two fragments (79 and 44 bp), whereas the PCR product with the Cys allele could not be cut by Tsp509I. To confirm detection of the C-1065A polymorphism of the apoM gene by PCR–RFLP, PCR products were purified with Centricon-100 filtration devices (Amicon, Beverly, MA, USA) and sequenced directly with a fluorescence-based automated DNA sequencer (Applied Biosystems, Foster City, CA, USA). All experiments were performed in duplicate in order to obtain precise assessments of genotype for each subject.
Measurement of plasma apoM concentration
Plasma apoM concentration was estimated by dot-blot analysis using monoclonal rabbit anti-human apoM antibody (ABNOVA). Briefly, 10 μl of plasma was diluted 1:20 in Tris–HCl buffer and 5 μl of the diluted samples was applied to a membrane in triplicate (Hybond-C, Amersham Life Science). Membranes were quenched in Tris–HCl buffer with 4% Tween and 3% bovine serum albumin for 2 hours, followed by sequential incubations with monoclonal rabbit anti-human apoM antibody (1:1000 in Tris–HCl buffer) overnight at 4°C, and then alkaline phosphatase (AP)-conjugated secondary antibody for 2 hours at room temperature. AP levels were measured with a commercial visualization system following the manufacturer’s instructions (Dako). The recombinant ApoM protein (ABNOVA) and ddH2O were used as positive and negative controls for evaluation, respectively. The relative amounts of apoM in diluted plasma samples were quantified by a scanner using the Quantity One software (Version 4.2.1, Bio- Rad Laboratories) and presented as adjusted volume (ODu.mm-2). Each experiment was repeated at least in triplicate.
Clinical data from CAD patients and control subjects was compared by the unpaired Student's t test or the Mann–Whitney U test. Allele frequencies were estimated by the gene-counting method, and the χ2 test was used to identify significant variations from the Hardy–Weinberg equilibrium. We employed two models to assess differences in allele frequencies and genotypes between CAD patients and controls: comparing allele frequencies in 2 × 2 contingency tables or genotypes in 2 × 3 contingency tables. We also performed multivariable logistic regression analysis to adjust for risk factors. CAD was assigned as a dependent variable, and the independent variables included age, body mass index, gender (male = 0, female = 1), smoking status (0 = nonsmoker, 1 = smoker), metabolic variables (0 = no history of hypertension, or no hypercholesterolemia; 1 = positive history), and apoM genotype. All genotypes were set as categorical variables (homozygote with major allele = 0; heterozygote = 1; homozygote with minor allele = 2). The OR and 95% CI were also calculated. Plasma apoM levels for the different genotypes were compared between two groups by Wilcoxon signed ranked test, and the significance threshold was set to P = 0.05. Statistical analysis was done using the SPSS statistical software package version 11.5.
*Department of Surgery, Affiliated Taixing People’s Hospital, Yangzhou Medical University, Taixing 225400, People's Republic of China. #Department of Cardiovascular Surgery, Affiliated Shanghai 1st People’s Hospital, Shanghai Jiaotong University, Shanghai 210008, People's Republic of China. †Department of Cardiovascular Surgery, Affiliated Wuxi People’s Hospital, Nanjing Medical University, Qingyang Road 299, Wuxi City, Jiangsu Province 214023, China. * †Department of Cardiovascular Surgery, National Center for Cardiovascular Disease, Beijing 200000, People's Republic of China. Co-First Author: Yi Zhou Ye.
This research project was supported by a research grant of Wuxi Science and Technology Commission (CSZ00N1203), a research grant of the Administrative Center of Wuxi’s Hospitals and a research grant of Shanghai Science and Technology Commission (114119b1600).
- Marenberg ME, Risch N, Berkman LF, Floderus B, de Faire U: Genetic susceptibility to death from coronary heart disease in a study of twins. N Engl J Med. 1994, 330: 1041-1046. 10.1056/NEJM199404143301503View ArticlePubMedGoogle Scholar
- Nora JJ, Lortscher RH, Spangler RD, Nora AH, Kimberling WJ: Genetic– epidemiologic study of early-onset ischemic heart disease. Circulation. 1980, 61: 503-508. 10.1161/01.CIR.61.3.503View ArticlePubMedGoogle Scholar
- Xu N, Dahlback B: A novel human apolipoprotein (apoM). J Biol Chem. 1999, 274: 31286-31290. 10.1074/jbc.274.44.31286View ArticlePubMedGoogle Scholar
- Wolfrum C, Poy MN, Stoffel M: Apolipoprotein M is required for prebeta- HDL formation and cholesterol efflux to HDL and protects against atherosclerosis. Nat Med. 2005, 11: 418-422. 10.1038/nm1211View ArticlePubMedGoogle Scholar
- Visvikis-Siest S, Marteau JB: Genetic variants predisposing to cardiovascular disease. Curr Opin Lipidol. 2006, 17: 139-151. 10.1097/01.mol.0000217895.67444.deView ArticlePubMedGoogle Scholar
- Niu N, Zhu X, Liu Y, Du T, Wang X, Chen D, Sun B, Gu HF, Liu Y: Single nucleotide polymorphisms in the proximal promoter region of apolipoprotein M gene (apoM) confer the susceptibility to development of type 2 diabetes in Han Chinese. Diabetes Metab Res Rev. 2007, 23: 21-25. 10.1002/dmrr.641View ArticlePubMedGoogle Scholar
- Jiao GQ, Yuan ZX, Xue YS, Yang CJ, Lu CB, Lu ZQ, Xiao MD: A prospective evaluation of apolipoprotein M gene T-778C polymorphism in relation to coronary artery disease in Han Chinese. Clin Biochem. 2007, 40: 1108-1112. 10.1016/j.clinbiochem.2007.04.023View ArticlePubMedGoogle Scholar
- Xu WW, Zhang Y, Tang YB, Xu YL, Zhu HZ, Ferro A, Ji Y, Chen Q, Fan LM: A genetic variant of apolipoprotein M increases susceptibility to coronary disease in a Chinese population. Clin Exp Pharmacol Physiol. 2008, 35: 546-551. 10.1111/j.1440-1681.2007.04822.xView ArticlePubMedGoogle Scholar
- Park YJ, Yoo SA, Lee JH, Chung YJ, Cho CS, Kim W: The ApoM polymorphism as a novel risk factor for dyslipidaemia in rheumatoid arthritis: a possible shared link between disease susceptibility and dyslipidaemia. Clin Exp Rheumatol. 2013, 31: 180-188.PubMedGoogle Scholar
- Topol EJ, Smith J, Plow EF, Wang QK: Genetic susceptibility to myocardial infarction and coronary artery disease. Hum Mol Genet. 2006, 15: 117-123. 10.1093/hmg/ddl183. 10.1093/hmg/ddl183View ArticleGoogle Scholar
- Banerjee I, Pandey U, Hasan OM, Parihar R, Tripathi V, Ganesh S: Association between inflamatory gene polymorphisms and coronary artery disease in an Indian population. J Thromb Thrombolysis. 2009, 27: 88-94. 10.1007/s11239-007-0184-8View ArticlePubMedGoogle Scholar
- Samani NJ: Molecular genetics of coronary artery disease: measuring the phenotype. Clin Sci. 1998, 95: 645-646. 10.1042/CS19980321View ArticlePubMedGoogle Scholar
- Christoffersen C, Nielsen LB, Axler O, Andersson A, Johnsen AH, Dahlbäck B: Isolation and characterization of human apolipoprotein M-containing lipoproteins. J Lipid Res. 2006, 47: 1833-1843. 10.1194/jlr.M600055-JLR200View ArticlePubMedGoogle Scholar
- Axler O, Ahnström J, Dahlbäck B: Apolipoprotein M associates to lipoproteins through its retained signal peptide. FEBS Lett. 2008, 582: 826-828. 10.1016/j.febslet.2008.02.007View ArticlePubMedGoogle Scholar
- Jungner I, Marcovina SM, Walldius G, Holme I, Kolar W, Steiner E: Apolipoprotein B and A-I values in 147576 Swedish males and females, standardized according to the World Health Organization-International Federation of Clinical Chemistry First International Reference Materials. Clin Chem. 1998, 44: 1641-1649.PubMedGoogle Scholar
- Wu X, Niu N, Brismar K, Zhu X, Wang X, Efendic S, Du T, Liu Y, Gu HF, Liu Y: Apolipoprotein M promoter polymorphisms alter promoter activity and confer the susceptibility to the development of type 1 diabetes. Clin Biochem. 2009, 42: 17-21. 10.1016/j.clinbiochem.2008.10.008View ArticlePubMedGoogle Scholar
- Hu HJ, Jin EH, Yim SH, Yang SY, Jung SH, Shin SH, Kim WU, Shim SC, Kim TG, Chung YJ: Common variants at the promoter region of the ApoM confer a risk of rheumatoid arthritis. Exp Mol Med. 2011, 43: 613-621. 10.3858/emm.2011.43.11.068PubMed CentralView ArticlePubMedGoogle Scholar
- John SW, Weitzner G, Rozen R, Scriver CR: A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acids Res. 1991, 19: 408- 10.1093/nar/19.2.408PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.