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Dietary marine-derived long-chain monounsaturated fatty acids and cardiovascular disease risk: a mini review
Lipids in Health and Disease volume 15, Article number: 201 (2016)
Regular fish/fish oil consumption is widely recommended for protection against cardiovascular diseases (CVD). Fish and other marine life are rich sources of the cardioprotective long-chain n-3 polyunsaturated fatty acids (n-3 PUFA) eicosapentaenoic acid (C20:5 n-3; EPA) and docosahexaenoic acid (C22:6 n-3; DHA). The lipid content and fatty acid profile of fish, however, vary greatly among different fish species. In addition to n-3 PUFA, certain fish, such as saury, pollock, and herring, also contain high levels of long-chain monounsaturated fatty acids (LCMUFA), with aliphatic tails longer than 18 C atoms (i.e., C20:1 and C22:1 isomers). Compared with well-studied n-3 PUFA, limited information, however, is available on the health benefits of marine-derived LCMUFA, particularly in regard to CVD. Our objective in this review is to summarize the current knowledge and provide perspective on the potential therapeutic value of dietary LCMUFA-rich marine oil for improving CVD risk factors. We will also review the possible mechanisms of LCMUFA action on target tissues. Finally, we describe the epidemiologic data and small-scaled clinical studies that have been done on marine oils enriched in LCMUFA. Although there are still many unanswered questions about LCMUFA, this appears to be promising new area of research that may lead to new insights into the health benefits of a different component of fish oils besides n-3 PUFA.
Cardiovascular disease (CVD) is the leading global cause of death, accounting for 17.3 million deaths per year, and is expected to grow to more than 23.6 million by 2030 . According to a recent American Heart Association analysis, 40.5% of the US population is projected to develop some form of CVD by 2030. Indirect costs from CVD are projected to increase by 61%, from $172 billion in 2010 to over $276 billion in 2030 . In addition, both metabolic syndrome and type 2 diabetes confer an increased risk of coronary heart disease and CVD [3, 4]. Thus, the mortality associated with CVD and its rising costs illustrate the importance of CVD prevention and the necessity for identifying effective ways to prevent its occurrence.
Fish and fish oil consumption has been shown in multiple studies to be related to reduced incidence of sudden cardiac death and total mortality [5–7]. Epidemiologic studies have also found that a high intake of small and medium size fish (mackerel, sardine, saury and eel) was associated with a lower risk of type 2 diabetes . Currently, the American Heart Association recommends eating fatty fish, such as salmon, mackerel, and herring, at least twice a week to promote heart health . Fish and fish oils are known to be enriched in long-chain n-3 polyunsaturated fatty acid (PUFA), such as eicosapentaenoic acid (C20:5 n-3; EPA) and docosahexaenoic acid (C22:6 n-3; DHA). The therapeutic use of n-3 PUFA has been extensively studied in a wide variety of disease conditions, including CVD. Potential mechanisms whereby n-3 PUFA may reduce risk for CVD include their beneficial effects on lipid and lipoprotein metabolism, blood pressure, platelet function, arterial cholesterol delivery, vascular function, and inflammatory responses [10, 11]. Fish lipids, however, also contain varying amounts of other unusual types of fatty acids (FA) that are not commonly found in other food sources . For example, saury , pollock , herring , capelin , and sprats , as well as marine mammals, such as seals and whales , are all enriched in long-chain monounsaturated fatty acids (LCMUFA) that originate from their food source, such as zooplankton [19, 20] (Table 1). LCMUFA are defined as monounsaturated fatty acid isomers with aliphatic tails at least 20 carbons (Table 2), with n-11 LCMUFA as the most abundant component. Although the n-9 LCMUFA isomers, gondoic acid (C20:1 n-9) and erucic acid and (C22:1 n-9), are also found in vegetable oils from mustard seed and rapeseed, the major LCMUFA enriched in marine sources are the n-11 series, gadoleic acid (C20:1 n-11) and cetoleic acid (C22:1 n-11) .
Saury is one of the most highly enriched fish in LCMUFA and is widely consumed in Asia . Herring, which is among the main commercial fish species in European countries, and Alaska pollock, which is the primary target for the fish industry in the United States, are also enriched in LCMUFA [23, 24]. In addition, some of the more widely consumed fish species, such as salmon and cod, also contain a significant amount of LCMUFA [14, 25]. Thus, a considerable amount of LCMUFA is consumed from various fish sources. Although numerous reports and reviews in recent years have showed beneficial effects of marine-derived n-3 PUFA on CVD and many other chronic diseases, only a limited number of studies have focused on the health impact of the consumption of LCMUFA. The purpose of this review is, therefore, to describe the experimental and clinical evidence on the effect of LCMUFA-rich diet on human health.
Incorporation of LCMUFA into plasma and organ lipids
Compared with n-11 LCMUFA (C20:1 n-11 and C22:1 n-11), which can only be derived from the diet, n-9 LCMUFA (C20:1 n-9 and C22:1 n-9) can also be formed by de novo synthesis, by the action of FA elongases on oleic acid (C18:1 n-9) . Although earlier animal studies showed that diets enriched in the LCMUFA isomer C22:1 caused a transient lipidosis in some organs, lipidosis disappeared upon continued feeding, possibly due to increased activity of peroxisomal β-oxidation . A recent animal feeding study from our group showed that the LCMUFA-rich diet resulted in a small but significant increase in each LCMUFA isomer in plasma and vital organs, such as liver, skeleton muscle, and duodenum, with the most prominent changes occurring in adipose tissues . Similarly, generally MUFA is also enriched in adipose tissue , because of either its greater entrance into adipocytes or because of a putative desaturation process of saturated FA by the steraoyl desaturase (SCD1). Compared with organ levels of LCMUFA, less LCMUFA are found in plasma, suggesting a possible rapid metabolism of these monoenoic acids. This hypothesis is supported by human studies. An early study conducted by von Lossonczy et al.  showed that the plasma LCMUFA was not detected in serum lipid fractions, such as TG and sterol esters, in healthy subjects fed mackerel diet for 3 weeks, despite of high content of LCMUFA (31% (w/w)) in the mackerel fat. In another study, plasma chylomicron cetoleic acid (C22:1 n-11) levels peaked at hour 3–6 (10.6% ± 4.5), and dropped to near baseline (0.03% ± 0.08) at 12–24 h (0.13% + 0.05) after fasting subjects consumed 50 ml of herring oil enrich in LCMUFA (22.3% cetoleic acid) . We also found that a single serving of LCMUFA-rich saury diet (11 g of LCMUFA and 5.6 g of n-3 PUFA in the saury meal) resulted in a rapid 13-fold increase in plasma LCMUFA levels 2 h after the meal and then declined sharply . The postprandial plasma levels of LCMUFA dropped significantly at 24 h, although it was still significantly higher than baseline (0.37% ± 0.02 at 24 h vs. 0.17% ± 0.05 at baseline, p < 0.01). In contrast, plasma n-3 PUFA levels peaked at 6 h and increased only by 1.1-fold after saury ingestion and then gradually declined. At 24 h post-ingestion, plasma n-3 PUFA levels rose by 67% compared with the pre-ingestion values (10% ± 0.6 at 24 h vs. 6% ± 0.7 at baseline, p < 0.01). Overall, these results suggest that different metabolic pathways may be responsible for the mobilization rates of different fish oil-derived fatty acids. In general, it appears that LCMUFA are rapidly catabolized and thus do not accumulate in plasma. Braekkan et al. also reported that no direct relation was found between the dietary fat types and fatty acid deposition . In contrast to fatty acids with shorter chain length and higher unsaturation that seemed to increase plasma deposition, LCMUFA had decreased plasma deposition probably due to rapid metabolism through retroconversion and or chain elongation .
LCMUFA-rich marine oil diet and CVD risk factors
Several animal studies have reported that LCMUFA–rich marine oil, improved CVD risk factors. A summary of these findings and the possible mechanism of action of LCMUFA on target tissues is shown in Fig. 1. For example, a high-fat fed C57BL/6J mice treated with saury oil for either short term (6-weeks) or long term (18-weeks) showed major improvements in several features related to metabolic syndrome [28, 34]. A 10% (w/w) of supplementation of saury oil (equivalent to appropriate 3.5% (w/w) LCMUFA) in a high-fat diet ameliorated diet-induced hyperinsulinemia and dyslipidemia compared to high-fat control diet. Saury oil diet resulted in a significant increase in LMCUFA levels, especially n-11 LCMUFA, in plasma and in organs (liver, adipose tissues and skeleton muscle). Suppression of genes related to adipogenesis and induction of genes involved in fatty acid oxidation and insulin signaling with saury oil supplementation were also associated with improvements in glucose and lipid metabolism. Similarly, 6-week treatment of 15% (w/w) of LCMUFA-rich pollock oil (equivalent to appropriate 3.9% (w/w) LCMUFA) in diet-induced obese mice increased organ levels of LCMUFA, and suppressed the rise in proatherogenic LDL-cholesterol without decreasing anti-atherogenic levels of HDL-cholesterol . An attenuation in hepatic steatosis and a down-regulation of hepatic genes involved in cholesterol and lipid synthesis by the pollock oil diet most likely contributed to these findings. In addition, Gabrielsson et al. fed LDLR-deficient mice herring fillet or beef for 16 weeks, and investigated the effect of dietary herring on plasma lipid levels and atherosclerosis . The major differences in fatty acid composition between herring and beef diet were the enrichment of long-chain n-3 PUFA (herring diet: 4.9% EPA and DHA vs. beef diet: Not Detected) and LCMUFA (herring diet: 3.4% C20:1 and C22:1 vs. beef diet: Not Detected) in herring diet. They showed that herring diet compared to the beef diet led to lower plasma triglyceride (TG) and Very-low-density lipoprotein (VLDL)-cholesterol levels and higher plasma High-density lipoprotein (HDL)-cholesterol levels, along with less atherosclerotic lesions. A recent study by Eilertsen et al. used marine mammal oil, and the results showed that atherogenesis was inhibited in apoE-deficient mice fed diet supplemented with 1% of seal oil combined with extra virgin oil (EVO/n-3), compared with diet supplemented with 1% corn oil or without any supplement (control). Besides long-chain omega-3 PUFA, such as EPA and DHA, the EVO/n-3 oil was also enriched in LCMUFA (C20:1 in EVO/n-3 diet was 2-fold higher than that in control or corn oil-rich diet), suggesting that in addition to n-3 PUFA that the LCMUFA in the seal oil may also contribute to the protection against atherosclerosis . In addition to the studies using LCMUFA-rich fish oils or marine mammal oils, some studies also focused on the health impact of zooplankton-derived oils. Calanus finmarchicus is the most abundant herbivorous zooplankton that that are enriched in both n-3 PUFA and LCMUFA . Several studies showed beneficial effect of dietary Calanus oil in CVD risk, such as reducing atherosclerotic plaque formation, abdominal fat accumulation and hepatic steatosis, and improving glucose tolerance in mice through multiple mechanisms, including regulation of inflammatory response-associated gene expression in livers and adipose tissues [39–41]. Nevertheless, because these marine oils also contain considerable amounts of n-3 PUFA and intake of these marine oils increased plasma and organ levels of EPA and DHA, one cannot exclude the possibility that the benefit from this diet was only due to n-3 PUFA consumption. Further animal studies using purified LCMUFA are necessary to better understand the functional relationships between dietary LCMUFA and CVD risk factors.
Dietary LCMUFA concentrate oil and CVD risk factors
Only a few studies have been conducted to investigate the impact of dietary marine-derived LCMUFA on metabolic disorders (Table 3). Our group concentrated LCMUFA (LCMUFA: 60~70%; total n-3 PUFA: <1%) from saury oil and estimated its effect in animal models on various metabolic and inflammatory parameters, as well as atherosclerosis. A 5% (w/w) LCMUFA supplemented western diet for 6-weeks in diet-induced obese mice improved insulin resistance and reduced blood lipids compared to western diet control. These changes were attributed to favorable alterations in the mRNA level of genes related to glucose/lipid metabolism and inflammation . Similarly, chow diet supplemented with 4% (w/w) LCMUFA concentrate for 8-weeks in type II diabetic mice alleviated several metabolic parameters compared to soybean oil diet . Along with reduced hyperinsulinemia, hyperleptinemia, and adipocyte size, the LCMUFA diet down-regulated inflammatory genes, and up-regulated Pparg and its target genes. Pparg, an isoform in the PPAR family, is known to play a crucial role in regulating adipocyte differentiation and fatty acid metabolism [44, 45]. The LCMUFA-induced improvements in diabetes may, therefore, be possibly related to the upregulation of Pparg and its target genes. Upregulation of PPAR family genes by dietary LCMUFA was also observed in ApoE-deficient mice and LDLR-deficient mice . Both a higher dose 5% (w/w) or a lower dose 2% (w/w) LCMUFA supplementation in western diet significantly suppressed atherosclerotic development and upregulated key gene expression in PPAR signaling pathway, such as Pparα and Pparγ. PPARs are known to protect against atherosclerosis partly by regulating inflammation and enhancing ABCA1-dependent cholesterol efflux from macrophages in the arterial wall . Our data showed that LCMUFA diet decreased plasma inflammatory cytokine levels, and enhanced cholesterol efflux of apoB-depleted plasma in LDLR-deficient mice fed a LCMUFA diet by the ABCA1 transporter. It is important to note that when 2% of olive oil enriched in shorter-chain MUFA oleic acid was supplemented in western diet, no significant difference was found in atherosclerosis between the olive oil group and western diet fed control group of LDLR-deficient mice, suggesting that the effect of MUFA on CVD risk may differ by carbon chain lengths. Further studies are needed to elucidate the potential regulatory effects of individual LCMUFA isomers on modulating PPAR levels.
Interestingly, intake of LCMUFA concentrate has been shown to increase plasma/hepatic EPA and DHA levels and decrease arachidonic acid (C20:4 n-6) levels, although there were only trace levels of long-chain n-3 PUFA in the LCMUFA concentrate oil (EPA and DHA <0.5%) [42, 43, 46]. Mobilization of PUFA after LCMUFA diet feeding was also observed by Halvorsen et al. . In this study, a 3-week feeding of LCMUFA concentrate oil to rats resulted in a doubling of the EPA/arachidonic acid ratio in liver and plasma phospholipids compared to lard diet. Arachidonic acid, one of the major components of n-6 PUFA that competes with n-3 PUFA for several physiological processes, is reported to increase inflammatory signals possibly through one of its myriad metabolites and has been associated with metabolic/cardiovascular disorders . A low n-3/n-6 PUFA ratio has also been shown to promote the pathogenesis of many diseases, including cardiovascular disease and inflammatory diseases . Thus, LCMUFA-rich marine oils may also be beneficial in regard to CVD by their effect on circulating and organ levels of PUFA.
Comparison of the effect of dietary n-3 PUFA-rich and LCMUFA-rich marine oils on CVD risk factors
Both long-chain n-3 PUFA and LCMUFA are the main fatty acid fractions in some fish species and marine mammals, and the individual actions of n-3 PUFA and LCMFUA need to be better understood. To date, only a few studies have compared the effect of marine-derived n-3 PUFA and LCMUFA on CVD risk factors in animal models. In two earlier studies [48, 51], rats were fed lard diet supplemented with 6.5% of concentrated fish oil-derived n-3 PUFA (85% EPA and DHA), LCMUFA (80% C20:1 and C22:1), or not (control) for 3-weeks. The peroxisomal β-oxidation in livers from mice in both n-3 PUFA and LCMUFA diet groups were significantly enhanced compared with that of the lard diet group, with the n-3 PUFA diet group showing a greater increase. Previous studies also showed stimulatory effect of LCMUFA-rich oils on peroxisomal β-oxidation in the heart . FA are known to be degraded by both mitochondrial (primarily FAs with carbon chain length shorter than C20) and peroxisomal β-oxidation (primarily FAs with carbon chain length greater than C20), and these two pathways are partly controlled at the gene regulatory level [53, 54]. The nuclear receptor PPARα has been reported to have an important role in the transcriptional control of genes involved in cardiac FA oxidation, and long-chain FA, such as EPA and DHA and their derivatives, serve as PPARα ligands [55, 56]. The upregulation of Pparα by dietary n-3 PUFA or LCMUFA, therefore, may account for the stimulation of peroxisomal FA oxidation. In another study investigating metabolism differences between dietary fish oil and seal oil, plasma and hepatic lipids and lipid peroxidation levels were markedly lower in hamsters fed seal oil-rich diet for 4-weeks compared to those fed fish oil . One of the distinct differences between fish oil and seal oil was the fatty acid composition. Seal oil contains much higher levels of MUFA compared to fish oil (50.6% of MUFA in seal oil vs. 22.2% in fish oil). Because a considerable amount of shorter-chain MUFA (C18:1 n-9 and C16:1 n-7) were also contained in the MUFA fraction of seal oil besides LCMUFA (C20:1 n-9), it will be important to delineate the individual effects of each FA on the regulation of lipid metabolism and oxidative stress.
We recently analyzed the plasma lipid change in mice fed a lard diet supplemented with n-3 PUFA (97% EPA), LCMUFA (57% C20:1 and C22:1), or not (control) for 8-weeks . Mice in both n-3 PUFA and LCMUFA diet groups showed significantly lower total cholesterol in plasma than mice in the lard diet group, and this effect was stronger in the n-3 PUFA diet group compared with the LCMUFA diet group. In contrast to EPA, which decreased plasma total cholesterol with a concomitant decrease in HDL-cholesterol, LCMUFA-rich diet did not decrease HDL-cholesterol. Although the lipoprotein response to LCMUFA has not been well investigated, several studies have shown that MUFA decrease plasma LDL-cholesterol without lowering HDL-cholesterol . Nevertheless, the mechanism for the effect of LCMUFA-rich fish oil on HDL cholesterol and other lipoprotein changes is not fully understood and further studies are needed.
To date there are no published clinical studies that have directly evaluated the role of LCMUFA on improving CVD risk. A small number of studies have examined the plasma lipid and lipoprotein response to marine diets enriched in both n-3 PUFA and LCMUFA. Osterud et al. estimated the health impact of dietary marine oils (whale oil, seal oil and cod liver oil) contained in typical Eskimo diet in healthy subjects (n = 134), with whale oil mostly enriched in LCMUFA (28% LCMUFA vs. 18.7% n-3 PUFA) and cod liver oil mostly enriched in n-3 PUFA (28.7% n-3 PUFA vs. 18.2% LCMUFA) . After 10-week intake of various oils (15 ml/day), whale oil consumption had the most profound effect on increasing plasma HDL-cholesterol compared to the baseline, along with a significant increase in plasma LCMUFA levels by 80% compared to control (no oil). In contrast, plasma TG decreased significantly only in cod liver oil group compared to the control, with most profound increased in plasma n-3 PUFA levels. In another study using marine mammal oil, healthy subjects (n = 19) consumed 20 g of vegetable oil (control) or seal oil enriched in omega-3 PUFA (26.2% (w/w) vs. 0.3% in control) and LCMUFA (15.2% (w/w) vs. 0.3% in control) for 42 days. Compared with control, the seal oil supplement group showed a modest beneficial effect on fibrinogen and protein C levels . Similarly, a study by Bakken et al. showed that 2-week seal oil supplement (15 ml per day) attenuated microbubble-induced platelet aggregation compared with baseline in 11 healthy volunteers . Besides marine mammal oils, several studies investigated the association between dietary fish oils enriched in n-3 PUFA and LCMUFA and CVD risk. A cross-over study by Childs and associates investigated the lipoprotein response to different types of fish oils (EPA-rich pollock oil and DHA-rich tuna and salmon blend oils) in healthy subjects . Normolipidemic subjects consumed a diet enriched in butter fat, pollock oil, or either tuna or salmon-blend oil for 3-weeks, with a 4-week washout period between treatments. Interestingly, although both tuna and salmon blend oils used in the study were enriched in DHA (14.4% (w/w) in tuna oil, 9.6% in salmon blend oil, and 2.5% in butter), HDL responded differently in the tuna and salmon diet groups. Compared with butter diet, total HDL-cholesterol and HDL2-cholesterol decreased on the tuna diet, whereas there was no change in HDL-cholesterol and HDL2-cholesterol increased slightly in the salmon diet group. One factor that could possibly be responsible for this difference was the higher amount of LCMUFA in salmon blend oil (25% LCMUFA in salmon blend oil vs. 5.1% in tuna oil). The authors assumed that LCMUFA, as a MUFA family member, may act on plasma HDL similarly to oleic acid, which decreases LDL-cholesterol without decreasing HDL-cholesterol in human. Herring fish also contains a large amount of LCMUFA in its fillets, byproducts, or oil, and a few studies investigated the effect of herring diet on cardiovascular disease risk factors [63, 64]. The results showed that compared with the diet of matched pork and chicken diets, a 6-week herring-rich diet significantly raised HDL-cholesterol in healthy obese subjects. Because the study focused on the physiologic effect of n-3 PUFA contained in herring, no data were shown on the change of plasma LCMUFA levels after herring consumption, thus no definitive conclusion could be drawn on the possible correlation between plasma HDL-cholesterol and the LCMUFA consumption. Nevertheless, an important observation was that the content of MUFA levels was 2-fold higher in herring dishes (~15% of energy) compared to those in reference dishes (<5% of energy). Future studies on LCMUFA-rich fish/fish oil diet, particularly examining the difference in the change of lipid and lipoprotein between LCMUFA-rich fish/fish oil diet, are necessary to advance this field.
The early epidemiologic studies starting in the 1960s, which first showed a lower incidence of coronary atherosclerosis and lower plasma lipid levels in certain ethnic groups, such as Greenland Eskimos, who ate a lot of fish [65, 66], also suggested a possible benefit from LCMUFA consumption. Analysis of fatty acid composition of the Eskimo food revealed that in addition to n-3 PUFA, dietary LCMUFA (C20:1 and C22:1) amount per day in a typical Eskimo food was significantly higher (13-fold) than that found in Danish control group . Besides EPA and DHA, a significantly larger proportion of circulating LCMUFA (C20:1) was found in the serum of the Greenland Eskimo living in Greenland compared to another control group of Eskimos living in Denmark or a reference group of Caucasian Danes . Recent studies on the association between red blood cell (RBC) fatty acid levels and incident coronary artery disease in the Physician’s Health Study also showed a strong inverse association between red blood cell LCMUFA consumption and coronary artery disease . The RBC content of C22:1 n-9 showed significant inverse associations with coronary artery disease, even after being adjusted for RBC n-3 PUFA, saturated fatty acids, and de novo derived MUFA (C16:1 n-7 and C18:1 n-9). A similar inverse association was also observed between RBC C20:1 n-9 and coronary artery disease, although the significance disappeared after correction for multiple comparisons. In another cohort-based analysis of dietary FA intake and paraoxonase 1 (PON1), dietary gadoleic aicd (C20:1 n-11) intake was positively associated with PON1 activity in 1548 participants from Carotid Lesion epidemiology and Risk (CLEAR) study, whereas PUFA (EPA and arachidonic acid) were negatively associated with PON1 activity . PON1 is believed to be a key cardioprotective factor on lipoproteins . PON1 protects LDL and HDL against oxidation, and lower serum PON1 levels were associated with higher susceptibility of LDL oxidation and atherosclerosis risk . MUFA are also positively associated with PON1 in mammals, and in vitro studies suggested that MUFA may bind to a protective site on PON1 and prevents its inactivation by oxidation .
Perreault et al. found that the relative percentage of C22:1 n-9 in serum were significantly lower in metabolically unhealthy obese participants compared to lean healthy and metabolically healthy obese participants in the Diabetes Risk Assessment study . Metabolically healthy obese individuals had BMIs similar to metabolically unhealthy obese individuals, but they had lower risk for complications, such as inflammation, type 2 diabetes and CVD [75, 76]. The lipid parameters and circulating inflammatory markers in metabolically healthy obese individuals more closely resembled that of lean healthy individuals compared with metabolically unhealthy individuals, which may partly be attributed to the favorable change in FA profile. Two epidemiologic studies, however, showed that higher circulating erucic acid, but not gadoleic acid, were significantly correlated with higher incident congestive heart failure in the population from Cardiovascular Health Study (CHS) and the Atherosclerosis Risk in Communities Study, Minnesota subcohort (ARIC), respectively . It is worth noting that the dominant constituents of marine-derived LCMUFA are n-11 LCMUFA, which are mainly found in marine source. The majority of LCMUFA that were examined in the observational studies were n-9 LCMUFA, which are generally found in both healthful and unhealthful food sources, such as fish, mustard, vegetable oils and processed meats . Furthermore, in addition to LCMUFA, serum levels of some other fatty acids also changed in these observational studies, thus making it difficult to elucidate the exact relationship between LCMUFA intake and CVD risk. In summary, the current data from observational and interventional studies on LCMUFA intake are suggestive of a possible beneficial effect from LCMUFA on CVD health but at this point are inconclusive.
Regular fish consumption is generally recommended to promote health, and fish oil is the most commonly used food supplement. Therefore, a more detailed understanding on the effect of the minor fatty acid components of fish oils, such as LCMUFA, on CVD risk factors is warranted. A limited number of in vivo animal studies have provided valuable evidence supporting the potential for LCMUFA-rich diet in the prevention of life-style related diseases, such as type 2 diabetes, metabolic syndrome, and atherosclerosis. A few human studies have also suggested a possible link between LCMUFA-rich diet and CVD risk protection, but further basic science and clinical research studies are needed to firmly establish the effect of LCMUFA-rich fish diet on atherosclerosis and CVD risk and its mechanisms of action.
Long-chain monounsaturated fatty acids
Monounsaturated fatty acids
Peroxisome proliferator-activated receptor
Polyunsaturated fatty acids
WHO Media Center: Cardiovascular Diseases (CVDs) Fact Sheet. http://www.who.int/mediacentre/factsheets/fs317/en/). Accessed 12 Oct 2016.
Heidenreich PA, Trogdon JG, Khavjou OA, Butler J, Dracup K, Ezekowitz MD, et al. Forecasting the future of cardiovascular disease in the United States: a policy statement from the American Heart Association. Circulation. 2011;123:933–44.
Mottillo S, Filion KB, Genest J, Joseph L, Pilote L, Poirier P, et al. The metabolic syndrome and cardiovascular risk a systematic review and meta-analysis. J Am Coll Cardiol. 2010;56:1113–32.
Buse JB, Ginsberg HN, Bakris GL, Clark NG, Costa F, Eckel R, et al. Primary prevention of cardiovascular diseases in people with diabetes mellitus: a scientific statement from the American Heart Association and the American Diabetes Association. Circulation. 2007;115:114–26.
Calder PC, Yaqoob P. Omega-3 (n-3) fatty acids, cardiovascular disease and stability of atherosclerotic plaques. Cell Mol Biol (Noisy-le-grand). 2010;56:28–37.
Chen GC, Yang J, Eggersdorfer M, Zhang W, Qin LQ. N-3 long-chain polyunsaturated fatty acids and risk of all-cause mortality among general populations: a meta-analysis. Sci Rep. 2016;6:28165.
Chiesa G, Busnelli M, Manzini S, Parolini C. Nutraceuticals and bioactive components from fish for dyslipidemia and cardiovascular risk reduction. Mar Drugs. 2016;14:113.
Nanri A, Mizoue T, Noda M, Takahashi Y, Matsushita Y, Poudel-Tandukar K, et al. Fish intake and type 2 diabetes in Japanese men and women: the Japan Public Health Center-based Prospective Study. Am J Clin Nutr. 2011;94:884–91.
Kris-Etherton PM, Harris WS, Appel LJ, American Heart Association. Nutrition committee: fish consumption, fish oil, omega-3 fatty acids, and cardiovascular disease. Circulation. 2002;106:2747–57.
Harris WS, Dayspring TD, Moran TJ. Omega-3 fatty acids and cardiovascular disease: new developments and applications. Postgrad Med. 2013;125:100–13.
Endo J, Arita M. Cardioprotective mechanism of omega-3 polyunsaturated fatty acids. J Cardiol. 2016;67:22–7.
Gruger E. Fatty acid composition of fish oils. Washington: U.S. Dept. of the Interior Fish and Wildlife Service Bureau of Commercial Fisheries: [Supt. of Docs., U.S.G.P.O.(distributor)]; 1967
Ota T, Takagi T, Kosaka S. Changes in lipids of young and adult saury cololabis saira (Pisces). Mar Ecol Prog Ser. 1980;3:11–7.
Oliveira ACM, Bechtel PJ. Lipid composition of Alaska pink salmon (Oncorhynchus gorbuscha) and Alaska walleye pollock (theragra chalcogramma). J Aquat Food Prod Tech. 2005;14:73–91.
Aidos I, van der Padt A, Luten JB, Boom RM. Seasonal changes in crude and lipid composition of herring fillets, byproducts, and respective produced oils. J Agric Food Chem. 2002;50:4589–99.
Bragadóttir M, Pálmadóttir H, Kristbergsson K. Composition and chemical changes during storage of fish meal from Capelin (Mallotus villosus). J Agric Food Chem. 2004;52:1572–80.
Saito H, Murata M. The high content of monoene fatty acids in the lipids of some midwater fishes: family Myctophidae. Lipids. 1996;31:757–63.
Shahidi F, Zhong Y. Marine mammal oils. In: Bailey’s industrial oil and fat products. 6th ed. 2005. p. 259–78.
Parrish CC, French VM, Whiticar MJ. Lipid class and fatty acid composition of copepods (Calanus finmarchicus, C. glacialis, Pseudocalanus sp., Tisbe furcata and Nitokra lacustris) fed various combinations of autotrophic and heterotrophic protists. J Plankton Res. 2012;34:56–75.
Bailey C, McMeans BC, Arts MT, Rush SA, Fisk AT. Seasonal patterns in fatty acids of Calanus hyperboreus (Copepoda, Calanoida) from Cumberland Sound, Baffin Island. Nunavut Mar Biol. 2012;159:1095–105.
Chowdhury K, Banu LA, Khan S, Latif A. Studies on the fatty acid composition of edible oil. Bangladesh J Sci Ind Res. 2007;42:311–6.
Cheung LK, Tomita H, Takemori T. Mechanisms of docosahexaenoic and eicosapentaenoic acid loss from pacific saury and comparison of their retention rates after various cooking methods. J Food Sci. 2016;81:C1899–907.
European Market Observatory for Fisheries and Aquaculture Products (EUMOFA). the EU fish market, 2015 Edition; 2015. https://www.eumofa.eu/documents/20178/66003/EN_The+EU+fish+market_Ed+2015.pdf/4cbd01f2-cd49-4bd1-adae-8dbb773d8519. Accessed 12 Oct 2016.
Bimbo AP. Alaska seafood byproducts: 2008 update on potential products, markets, and competing products. In: Bechtel PJ, Smiley S, editors. A sustainable future: fish processing byproducts. Fairbanks: University of Alaska Fairbanks; 2010. p. 9–25.
Bratu A, Mihalache M, Hanganu A, Chira NA, Todasca MC, Rosca S. Quantitative determination of fatty acids from fish oils using GC-MS method and 1H-NMR spectroscopy. UPB Sci Bull Series B. 2013;75:23–32.
Suburu J, Gu Z, Chen H, Chen W, Zhang H, Chen YQ. Fatty acid metabolism: implications for diet, genetic variation, and disease. Food Biosci. 2013;4:1–12.
Bremer J, Norum KR. Metabolism of very long-chain monounsaturated fatty acids (22:1) and the adaptation to their presence in the diet. J Lipid Res. 1982;23:243–56.
Yang ZH, Inoue S, Taniguchi Y, Miyahara H, Iwasaki Y, Takeo J, et al. Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids. Lipids Health Dis. 2015;14:155.
Bergouignan A, Momken I, Schoeller DA, Simon C, Blanc S. Metabolic fate of saturated and monounsaturated dietary fats: the Mediterranean diet revisited from epidemiological evidence to cellular mechanisms. Prog Lipid Res. 2009;48:128–47.
von Lossonczy TO, Ruiter A, Bronsgeest-Schoute HC, van Gent CM, Hermus RJ. The effect of a fish diet on serum lipids in healthy human subjects. Am J Clin Nutr. 1978;31:1340–6.
Gowen DR, Connor WE, Corliss JD, Barstud LA. Cetoleic acid in human chylomicrons after herring oil meals. Clin Res. 1987;35:771A(abstr).
Yang ZH, Miyahara H, Takeo J, Katayama M. Ingestion of a single serving of saury alters postprandial levels of plasma n-3 polyunsaturated fatty acids and long-chain monounsaturated fatty acids in healthy human adults. Lipids Health Dis. 2012;11:95.
Braekkan OR, Lambertsen G, Utne F, Njaa LR. Hydrogenated marine fat, its influence on the fatty acid composition of depot fats and liver lipids in the rat. Nutr Dieta Eur Rev Nutr Diet. 1968;10:24–44.
Yang ZH, Miyahara H, Takemura S, Hatanaka A. Dietary saury oil reduces hyperglycemia and hyperlipidemia in diabetic KKAy mice and in diet-induced obese C57BL/6J mice by altering gene expression. Lipids. 2011;46:425–34.
Yang ZH, Miyahara H, Takeo J, Hatanaka A, Katayama M. Pollock oil supplementation modulates hyperlipidemia and ameliorates hepatic steatosis in mice fed a high-fat diet. Lipids Health Dis. 2011;10:189.
Gabrielsson BG, Wikström J, Jakubowicz R, Marmon SK, Carlsson NG, Jansson N, et al. Dietary herring improves plasma lipid profiles and reduces atherosclerosis in obese low- density lipoprotein receptor-deficient mice. Int J Mol Med. 2012;29:331–7.
Eilertsen KE, Mæhre HK, Cludts K, Olsen JO, Hoylaerts MF. Dietary enrichment of apolipoprotein E-deficient mice with extra virgin olive oil in combination with seal oil inhibits atherogenesis. Lipids Health Dis. 2011;10:41.
Skjoldal H, Sætre R, Færnö A, Misund O, Røttingen I, editors. The Nor-wegian Sea ecosystem. Trondheim: Tapir Academic Press; 2004.
Eilertsen KE, Mæhre HK, Jensen IJ, Devold H, Olsen JO, Lie RK, et al. A wax ester and astaxanthin-rich extract from the marine copepod Calanus finmarchicus attenuates atherogenesis in female apolipoprotein E-deficient mice. J Nutr. 2012;142:508–12.
Höper AC, Salma W, Khalid AM, Hafstad AD, Sollie SJ, Raa J, et al. Oil from the marine zooplankton Calanus finmarchicus improves the cardiometabolic phenotype of diet-induced obese mice. Br J Nutr. 2013;110:2186–93.
Höper AC, Salma W, Sollie SJ, Hafstad AD, Lund J, Khalid AM, et al. Wax esters from the marine copepod Calanus finmarchicus reduce diet-induced obesity and obesity-related metabolic disorders in mice. J Nutr. 2014;144:164–9.
Yang ZH, Miyahara H, Mori T, Doisaki N, Hatanaka A. Beneficial effects of dietary fish-oil- derived monounsaturated fatty acids on metabolic syndrome risk factors and insulin resistance in mice. J Agric Food Chem. 2011;59:7482–9.
Yang ZH, Miyahara H, Iwasaki Y, Takeo J, Katayama M. Dietary supplementation with long-chain monounsaturated fatty acids attenuates obesity-related metabolic dysfunction and increases expression of PPAR gamma in adipose tissue in type 2 diabetic KK-Ay mice. Nutr Metab (Lond). 2013;10:16.
Siersbaek R, Nielsen R, Mandrup S. PPARgamma in adipocyte differentiation and metabolism--novel insights from genome-wide studies. FEBS Lett. 2010;584:3242–9.
Varga T, Czimmerer Z, Nagy L. PPARs are a unique set of fatty acid regulated transcription factors controlling both lipid metabolism and inflammation. Biochim Biophys Acta. 2011;1812:1007–22.
Yang ZH, Bando M, Sakurai T, Chen Y, Emma-Okon B, Wilhite B, et al. Long-chain monounsaturated fatty acid-rich fish oil attenuates the development of atherosclerosis in mouse models. Mol Nutr Food Res. 2016;0:1–11.
Moraes LA, Pigueras L, Bishop-Bailey D. Peroxisome proliferator-activated receptors and inflammation. Pharmacol Ther. 2006;10:371–85.
Halvorsen B, Rustan AC, Madsen L, Reseland J, Berge RK, Sletnes P, et al. Effects of long- chain monounsaturated and n-3 fatty acids on fatty acid oxidation and lipid composition in rats. Ann Nutr Metab. 2001;45:30–7.
Sugimoto MA, Sousa LP, Pinho V, Perretti M, Teixeira MM. Resolution of inflammation: what controls its onset? Front Immunol. 2016;7:160.
Das UN. Essential fatty acids and their metabolites could function as endogenous HMG-CoA reductase and ACE enzyme inhibitors, anti-arrhythmic, anti-hypertensive, anti-atherosclerotic, anti-inflammatory, cytoprotective, and cardioprotective molecules. Lipids Health Dis. 2008;7:37.
Halvorsen B, Rustan AC, Christiansen EN. Effect of long-chain mono-unsaturated and n-3 polyunsaturated fatty acids on postprandial blood and liver lipids in rats. Scand J Clin Lab Invest. 1995;55:469–75.
Flatmark T, Christiansen EN. Modulation of peroxisomal biogenesis and lipid metabolizing enzymes by dietary factors. In: Lake B, Gibson G, editors. Peroxisomes: biology and importance in toxicology and medicine. London: Taylor and Francis; 1993. p. 247–75.
Camões F, Bonekamp NA, Delille HK, Schrader M. Organelle dynamics and dysfunction: a closer link between peroxisomes and mitochondria. J Inherit Metab Dis. 2009;32:163–80.
Latruffe N, Cherkaoui Malki M, Nicolas-Frances V, Clemencet MC, Jannin B, Berlot JP. Regulation of the peroxisomal beta-oxidation-dependent pathway by peroxisome proliferator- activated receptor alpha and kinases. Biochem Pharmacol. 2000;60:1027–32.
Ajith TA, Jayakumar TG. Peroxisome proliferator-activated receptors in cardiac energy metabolism and cardiovascular disease. Clin Exp Pharmacol Physiol. 2016;43:649–58.
Nakamura MT, Yudell BE, Loor JJ. Regulation of energy metabolism by long-chain fatty acids. Prog Lipid Res. 2014;53:124–44.
Dubey P, Jayasooriya AP, Cheema SK. Diets enriched in fish-oil or seal-oil have distinct effects on lipid levels and peroxidation in BioF1B hamsters. Nutr Metab Insights. 2011;4:7–17.
Yanai H, Katsuyama H, Hamasaki H, Abe S, Tada N, Sako A. Effects of dietary fat intake on HDL metabolism. J Clin Med Res. 2015;7:145–9.
Osterud B, Elvevoll E, Barstad H, Brox J, Halvorsen H, Lia K, et al. Effect of marine oils supplementation on coagulation and cellular activation in whole blood. Lipids. 1995;30:1111–8.
Conquer JA, Cheryk LA, Chan E, Gentry PA, Holub BJ. Effect of supplementation with dietary seal oil on selected cardiovascular risk factors and hemostatic variables in healthy male subjects. Thromb Res. 1999;96:239–50.
Bakken AM, Hervig T, Thorsen T, Holmsen H. Fatty acids in human platelets and plasma. dietary seal oil decreases sensitivity toward microbubbles. Platelets. 1995;6:259–64.
Childs MT, King IB, Knopp RH. Divergent lipoprotein responses to fish oils with various ratios of eicosapentaenoic acid and docosahexaenoic acid. Am J Clin Nutr. 1990;52:632–9.
Lindqvist H, Langkilde AM, Undeland I, Rådendal T, Sandberg AS. Herring (Clupea harengus) supplemented diet influences risk factors for CVD in overweight subjects. Eur J Clin Nutr. 2007;61:1106–13.
Lindqvist HM, Langkilde AM, Undeland I, Sandberg AS. Herring (Clupea harengus) intake influences lipoproteins but not inflammatory and oxidation markers in overweight men. Br J Nutr. 2009;101:383–90.
McGandy RB, Hegsted DM, Stare FJ. Dietary fats, carbohydrates and atherosclerotic vascular disease. N Engl J Med. 1967;277:245–7.
Bang HO, Dyerberg J, Nielsen AB. Plasma lipid and lipoprotein pattern in Greenlandic West-coast Eskimos. Lancet. 1971;1:1143–5.
Bang HO, Dyerberg J, Sinclair HM. The composition of the Eskimo food in north western Greenland. Am J Clin Nutr. 1980;33:2657–61.
Dyerberg J, Bang HO, Hjorne N. Fatty acid composition of the plasma lipids in Greenland Eskimos. Am J Clin Nutr. 1975;28:958–66.
Matsumoto C, Matthan NR, Lichtenstein AH, Gaziano JM, Djoussé L. Red blood cell MUFAs and risk of coronary artery disease in the Physicians’ Health Study. Am J Clin Nutr. 2013;98:749–54.
Kim DS, Maden SK, Burt AA, Ranchalis JE, Furlong CE, Jarvik GP. Dietary fatty acid intake is associated with paraoxonase 1 activity in a cohort-based analysis of 1,548 subjects. Lipids Health Dis. 2013;12:183.
Soran H, Schofield JD, Liu Y, Durrington PN. How HDL protects LDL against atherogenic modification: paraoxonase 1 and other dramatis personae. Curr Opin Lipidol. 2015;26:247–56.
Tsuzura S, Ikeda Y, Suehiro T, Ota K, Osaki F, Arii K, et al. Correlation of plasma oxidized low-density lipoprotein levels to vascular complications and human serum paraoxonase in patients with type 2 diabetes. Metabolism. 2004;53:297–302.
Nguyen SD, Sok DE. Beneficial effect of oleoylated lipids on paraoxonase 1: protection against oxidative inactivation and stabilization. Biochem J. 2003;375:275–85.
Perreault M, Zulyniak MA, Badoud F, Stephenson S, Badawi A, Buchholz A, et al. A distinct fatty acid profile underlies the reduced inflammatory state of metabolically healthy obese individuals. PLoS One. 2014;9:e88539.
Blüher M. The distinction of metabolically ‘healthy’ from ‘unhealthy’ obese individuals. Curr Opin Lipidol. 2010;21:38–43.
Karelis AD, Faraj M, Bastard JP, St-Pierre DH, Brochu M, Prud’homme D, et al. The metabolically healthy but obese individual presents a favorable inflammation profile. J Clin Endocrinol Metab. 2005;90:4145–50.
Imamura F, Lemaitre RN, King IB, Song X, Steffen LM, Folsom AR, et al. Long-chain monounsaturated Fatty acids and incidence of congestive heart failure in 2 prospective cohorts. Circulation. 2013;127:1512–21.
The authors acknowledge financial support by the Intramural Research Program of the National Heart, Lung and Blood Institute (NHLBI) and the Office of Dietary Supplements (ODS) Research Scholars Program at National Institutes of Health.
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All authors made substantial contributions to this manuscript. ZHY and AR designed and outlined the manuscript. ZHY conducted the literature research and wrote the manuscript. BO and AR supported the literature research, drafting and final corrections of the manuscript. All authors approved the final manuscript.
The authors declare that they have no competing interests.
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Yang, ZH., Emma-Okon, B. & Remaley, A.T. Dietary marine-derived long-chain monounsaturated fatty acids and cardiovascular disease risk: a mini review. Lipids Health Dis 15, 201 (2016). https://doi.org/10.1186/s12944-016-0366-5
- Long-chain monounsaturated fatty acids
- Cardiovascular disease
- Peroxisome proliferator-activated receptor signaling pathway