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Mediation analysis of erythrocyte lipophilic index on the association between BMI and risk of oral cancer

Lipids in Health and Disease volume 21, Article number: 96 (2022) Cite this article

Abstract

Aims

To explore the relationship between the fatty acid lipophilic index (LI) of the erythrocyte membrane and oral cancer risk, as well as to evaluate the possibility of LI acting as a mediator of the association between body mass index (BMI) and oral cancer.

Method

Twenty-three fatty acids (FAs) of the erythrocyte membrane were measured using gas chromatography in 380 patients with oral cancer and 387 control subjects. The LI was calculated based on the FA proportion and FA melting points. The association of BMI and erythrocyte LI with oral cancer risk was analysed using logistic regression. The mediation effect of LI on the association between BMI and oral cancer risk was evaluated using mediation analysis.

Results

Among the control group, 46.0% were overweight or obese, which was significantly higher than that of oral cancer patients (29.5%). Significant differences in erythrocyte membrane saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs) were observed between the patient and control groups. The proportion of C18:1 n-9 from the MUFA family increased in oral cancer patients (12.67%) compared with controls (12.21%). While the total proportion of n-3 PUFAs decreased in oral cancer patients compared with controls, with C20:5 n-3 decreasing from 0.66 to 0.47%, and C22:6 n-3 decreasing from 5.82 to 4.86%. The LI was lower in the control participants (M = 27.6, IQR: 27.3–27.9) than in the oral cancer patients (M = 28.2, IQR: 27.9–28.5). BMI was inversely associated with oral cancer risk with a fully adjusted OR of 0.59 (95% CI: 0.43–0.83), while LI was positively associated with oral cancer risk with a fully adjusted OR of 1.99 (95% CI:1.36–2.94). LI explained 7% of the variance in the relationship between BMI and oral cancer risk.

Conclusions

The distribution of the FA profile in erythrocyte membranes differed between the oral cancer patients and the control group. The LI derived from the profile of FAs was positively associated with the risk of oral cancer, and the associations between BMI and oral cancer risk can be explained, at least in part, by LI.

Introduction

Oral cancers, are a common cancer type globally, and approximately 377,713 new cases occur every year [1]. In China, oral cancer was frequently overlooked by governments in public health policy, although it had emerged as a substantial component of worldwide oral health issues, with considerable societal and economic consequences [2]. In addition to smoking, drinking, poor oral hygiene, and human papillomavirus, which have all been identified as causative factors in oral cancer [3,4,5], studies have also reported that BMI plays a crucial role in oral cancer [6,7,8]. Changes in BMI have been shown to affect metabolic lipid profiles and studies have indicated that obesity is a product of metabolic imbalance, as well as a driver of fatty acid (FA) metabolism disturbance [9]. Elevated levels of free FA and dyslipidemia are common in obesity. Obesity-related genes and lipid signaling are dysregulated in the microenvironment, exacerbating lipid metabolism problems and facilitating tumor proliferation and development in obese patients [10, 11].

FA composition in the erythrocyte membrane is regarded as a comprehensive biomarker of the homeostatic condition of FA. FA composition in membranes determines the fluidity of the membranes, which subsequently impacts the capacities of membrane-bound proteins and cells [12]. Moreover, the FA profile of the erythrocyte membrane is representative of the general condition of other tissues which coordinates the complex interaction between dietary intake of FAs and endogenous FA metabolism [13]. In several studies, abnormalities in FA profiles have been reported to be linked to chronic diseases including cancers [14,15,16]. Furthermore, a few studies have looked at the impact of changing FA levels on the etiology of malignancies, using the erythrocyte membrane as a possible biomarker [16,17,18,19,20]. The lipophilic index (LI), which is a measurement of the lipophilic appeal of different FAs, may be determined primarily from the FA composition of biological membranes. The LI was defined as the average of the melting points of individual FAs multiplied by the corresponding proportion in the membrane. Previous studies have discovered the relationship between LI and chronic diseases such as diabetes and coronary heart disease [13, 21, 22].

In the current study, the association between BMI, erythrocyte membrane LI and oral cancer, as well as whether LI could mediate the association between BMI and oral cancer risk, were explored.

Materials and methods

Study population and data collection

A hospital-based case-control study was conducted with patients from the First Affiliated Hospital of Fujian Medical University from September 2013 to December 2020 in Fujian Province, China, as previously described [23]. Patients with newly diagnosed oral cancer (C00-C07 corresponding to the 10th revision of the International Classification of Diseases) were enrolled in this study and serum samples were collected at admission. The criteria for inclusion were as follows: (1) primary oral cancer diagnosed histologically; (2) Chinese population with at least 10 years of residency in Fujian Province; and (3) age between 20 and 80 years. Those with recurring or metastasized cancer, as well as those who had already received chemotherapy or radiation, were excluded. During the same period, control subjects were recruited from the physical examination center of the same hospital. Any additional malignancies that were present or had been present were excluded. Finally, 380 patients and 387 healthy participants were admitted into the study.

The study protocol was accepted by Fujian Medical University’s Ethics Committee (Approval number:2011053; Approval date: March 10, 2011) and followed the Declaration of Helsinki’s ethical standards. All participants gave their informed consent. For both the case and control groups, demographic data were obtained by face-to-face interviews using a standardized questionnaire.

BMI assessment

Height and weight were measured by the nurse of the hospital at admission. Body mass index (BMI) was calculated as weight (in kilograms) divided by the square of the height (in meters) and was classified into three categories (< 18.5 /18.5 ~ 23.9 /≥24).

Measurement of the FA profile of erythrocytes membranes and calculation of LI

Blood samples from the study population in a fasting state at their first admission were collected. Venous blood samples (approximately 5 mL) collected in vacutainer tubes were used to determine the FA composition of mature erythrocyte membrane phospholipids. The samples were kept at − 80 °C until they were analyzed.

Details of the erythrocyte membrane FA acquisition and measurement have been described previously [23]. Briefly, FA methyl esters were separated using an Agilent 7890B gas chromatograph (Agilent, Palo Alto, CA, USA) equipped with a DB-23 fame column (60 m × 0.25 mm inside diameter, the film thickness of 0.15 μm) for flame ionization. A mixed FA methyl ester standard (Sigma-Aldrich Inc., USA) was used for the separation of FA methyl ester peaks. A total of 38 erythrocyte membrane FAs were tested, and 23 erythrocyte membrane FAs were above the detection limit. The FA composition is presented as the percentage of individual FAs divided by the total FAs.

The LI was calculated using the following equation [21]:

$$\mathrm{Lipophilic}\ \mathrm{index}=\frac{\ \sum_k\Big[ Levels\ of\ fatty\ acid\left(\%\right)i\times melting\ point\left({}^{{}^{\circ}}C\right)i\Big]}{\sum_k\left[ Levels\ of\ fatty\ acid\left(\%\right)i\right]}$$

where i denotes individual FAs and k is the total number of FAs utilized to compute LI. This index reflects the overall FA lipophilicity in erythrocytes.

The LipidBank database (http://lipidbank.jp/) was used to obtain the melting points of FAs. Of the 23 quantified individual FAs in erythrocytes, data on melting points were available for 20. Hence, 20 FAs were included to calculate the LI (8% of measured FAs not considered, 92% of measured FAs considered).

Covariate analysis

Two educational level groups were defined: the low group (lower vocational training or primary school) and the high group (secondary school and above). Demographic factors included age (< 60 years/≥60 years), sex (male/female), residence (rural/urban), marital status (married/others), education (low/high), occupational activity (light, moderate, or heavy) [24], family history of cancers (no/yes), smoking status (no/yes), alcohol drinking (no/yes), diabetes (no/yes) and oral hygiene (well/poor).

Statistical analysis

The distribution of demographic characteristics between the case and control groups was examined using the χ2 test or Fisher’s exact test. The distribution of FAs between patients and control participants was analyzed using the Wilcoxon rank-sum test and visualized by a violin plot. The correlations between individual FAs and the LI were investigated using Spearman’s correlation coefficients.

The association between BMI, erythrocyte membrane LI and oral cancer was analyzed using an unconditional logistic regression model with BMI and LI being treated as both categorical and continuous variables. ORs and 95% CI were calculated. In addition to the crude model, age, sex, education, residence, marital status, occupational activity, and family history of cancer were adjusted for in Model 2. Model 3 was further adjusted for smoking status, alcohol drinking, oral hygiene, and history of diabetes. Mediation analysis was performed to test the mediation effect of LI on the association between BMI and oral cancer using the medflex package in R. Covariates in mediation analysis were obtained using a generalized linear model with the mediated model (BMI → LI) and outcome model (LI and BMI → oral cancer). Analysis of the study was performed in R version 4.1.0.

Results

The baseline characteristics of the study population are shown in Table 1. There were 387 control subjects and 380 patients in the study. The mean BMIs of the control and oral cancer cases were 23.78 kg/m2 and 22.25 kg/m2, respectively. In the control group, the proportion of overweight or obese was 46.0%, while in the oral cancer group, the proportion of overweight or obese was 29.5%. No statistically significant differences were observed in the distribution of sex, residence, marital status, or occupational activity between the patient and control groups (all P > 0.05). Compared with the control group, the case group was characterized by a higher proportion of subjects with smoking, drinking, and family history of cancer.

Table 1 Baseline characteristic of the study participants
Full size table

The melting points, proportions of each erythrocyte membrane FA, and Spearman’s correlation coefficients between the individual FAs and LI were listed in Table 2. The major FA components of the erythrocyte membrane were C16:0 (22.83%), C18:0 (12.40%), C18:1 (12.38%), C18:2 (12.48%) and C20:4 (13.31%). Generally, erythrocyte LI was positively correlated with SFAs, including C16:0, C18:0, C20:0, C22:0, C23:0, and C24:0, and MUFAs, including C18:1 n-9, C22:1 n-13, C24:1 n-15 (all r > 0, P < 0.05). However, PUFAs, including C20:5 n-3, C22:5 n-3, and C22:6 n-3, were inversely correlated with the LI (all r < 0, P < 0.05).

Table 2 Fatty acid melting points, erythrocyte fatty acid mean proportions, and erythrocyte FA correlation coefficients with the Lipophilic Index (LI)
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The FA composition of erythrocyte membranes in the patient and control groups was shown in Fig. 1. Significant differences in saturated FAs (including C16:0, C22:0, C23:0, and C24:0), monounsaturated FAs (including C16:1 n-9 and C18:1 n-9), n-6 polyunsaturated FAs (including C18:2 n-6) and all n-3 polyunsaturated FAs (including C18:3 n-3, C20:5 n-3, C22:5 n-3, and C22:6 n-3) were observed between the two groups (all P < 0.05). The proportion of C18:1 n-9 from the MUFA family was increased in oral cancer patients (12.67%) compared with controls (12.21%). The total proportion of n-3 PUFAs was decreased in oral cancer patients compared with controls, with C20:5 n-3 decreasing from 0.66 to 0.47% and C22:6 n-3 decreasing from 5.82 to 4.86%.

Fig. 1
figure 1

Distribution of fatty acids between patients and control participants (*Correlation was significant at the 0.05 level; ** Correlation was significant at the 0.01 level)

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The distribution of LI was analyzed according to disease status, age, BMI, smoking status, alcohol drinking, and oral hygiene. As shown in Fig. 2, LI was higher in the patient group (M = 27.6, IQR: 27.3–27.9) than in the control group (M = 28.2, IQR: 27.9–28.5). The results showed that younger subjects had a higher LI than older subjects. The distribution of LI was also significantly different across different smoking, alcohol drinking, and oral hygiene statuses.

Fig. 2
figure 2

Classified scatter plots of the lipophilic index according disease status, age, BMI, smoking status, alcohol drinking, and oral hygiene (A) by disease status (Median(IQR): 26.9(25.8,28.1) vs. 27.7(26.2,27.7) (B) by age (Median(IQR): 27.7(26.3,30.3) vs. 26.9(25.7,28.2) (C) by BMI (Median(IQR): 27.2(26.1,29.6) vs. 27.3(26.1,29.1) vs. 27.2(25.9,28.8) (D) by smoking status (Median(IQR): 27.1(25.8,28.8) vs. 27.4(26.2,29.6) (E) by alcohol drinking (Median(IQR): 27.1(25.8,28.8) vs. 27.5(26.4,29.5) (F) oral hygiene (Median(IQR): 27.6(26.2,29.7) vs. 27.0(25.8,28.7)

Full size image

Next, logistic regression was performed to assess the association between BMI, LI and oral cancer. As shown in Table 3, an inverse association between BMI and oral cancer risk was observed when BMI was treated as a continuous variable in Model 1, Model 2, and Model 3 (OR = 0.84, 95% CI: 0.80–0.88; OR = 0.84, 95% CI: 0.79–0.89; OR = 0.85, 95% CI: 0.80–0.89). Compared with normal BMI (18.5 ~ 23.9), the ORs of BMI < 18.5 kg/m2 and BMI ≥24 kg/m2 were 2.37 (95% CI: 1.25–4.50) and 0.54 (95% CI: 0.39–0.73), respectively, in Model 1. After adjustment for age, sex, residence, marital status, occupational activity and family history of cancer in Model 2, the association of BMI with oral cancer risk was more significant for BMI < 18.5 kg/m2 (OR = 2.45, 95% CI: 1.26–4.76) and BMI ≥24 kg/m2 (OR = 0.56, 95% CI: 0.41–0.78). In Model 3, where smoking, alcohol consumption, oral hygiene, LI, and diabetes were further adjusted, the effects of BMI < 18.5 kg/m2 (OR = 2.47, 95% CI: 1.25–4.89) and BMI > 24 kg/m2 (OR = 0.59, 95% CI: 0.43–0.83) on oral cancer risk were still significant. The association between LI and oral cancer was also examined using logistic regression. In the crude model, a higher risk of oral cancer was observed in the top tertile (OR = 2.27, 95% CI:1.59–3.24, Ptrend < 0.001) than in the bottom tertile. This association was slightly attenuated in Model 2 (OR = 2.08, 95% CI: 1.45–2.99, Ptrend < 0.001). The association was also slightly attenuated, but the association was still statistically significant after further adjusting for smoking, drinking, oral hygiene, BMI, and diabetes (OR = 1.99, 95% CI: 1.36–2.94, Ptrend < 0.001). A positive and significant association was observed when LI was treated as a continuous variable in the crude model, Model 1 (OR = 1.07, 95% CI: 1.02–1.12; OR = 1.05, 95% CI: 1.01–1.11).

Table 3 Association between BMI, lipophilic index and risk of oral cancer
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The association between BMI and oral cavity cancer by stratified analysis was shown in Fig. 3. An inverse and significant association was observed between BMI and oral cancer when stratified by smoking status, and a strong effect was observed in smokers (ORtertile3v.1 = 0.70, 95% CI: 0.52–0.94). Associations of BMI and oral cancer remained significant among subjects ≥60 years (ORtertile3v.1 = 0.66, 95% CI: 0.53–0.82) and among subjects with poor oral hygiene (ORtertile3v.1 = 0.70, 95% CI: 0.57–0.85) compared with subjects aged < 60 years and subjects with better oral hygiene. When stratified by LI, an inverse and significant association was only observed in subjects with high LI (ORtertile3v.1 = 0.70, 95% CI: 0.52–0.94), and no interaction was shown between BMI and LI (Pinteraction = 0.998). Moreover, the results of the stratified analysis between LI and oral cancer were shown in Supplementary Fig. 1. There was a positive association between LI and oral cancer when stratified by BMI and alcohol drinking and a strong effect between LI and oral cancer risk was observed among overweight or obese participants (ORtertile3v.1 = 1.46, 95% CI: 1.05–2.03). In addition, a stronger association was observed in non-smokers (ORtertile3v.1 = 1.52, 95%CI: 1.19–1.94) than in subjects who smoked.

Fig. 3
figure 3

Association between the BMI and oral cancer by stratified analysis

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The results of selecting covariates in mediation analysis were shown in Supplement Table 1. The multivariable analysis suggested that BMI, sex, age, and occupational activity were associated with LI, and the outcome model for oral cancer indicated that education, alcohol drinking, family history of cancer, and diabetes were significant. These significant covariates in the two models were considered simultaneously in the mediation analysis. Finally, the mediation effect of LI on the association between BMI and oral cancer risk was shown in Table 4. The results showed that LI could mediate the association between BMI and oral cancer with a significant percentage of 7%. The direct effects (BMI → oral cancer) and indirect effects (BMI → LI → Oral cancer) were presented as ORs of 0.884 (95% CI: 0.801, 0.889) and 0.986 (95% CI: 0.973,0.998), respectively.

Table 4 The effects of the BMI on oral cancer risk through lipophilic index
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Discussion

The results of the current case–control study indicated that the oral cancer group was more likely to have a low proportion of overweight or obese patients than the control group, and the distribution of the FA profile in erythrocyte membranes differed between oral cancer patients and control participants. Erythrocyte membrane LI, which was derived from both FA melting points and FA composition, was associated with the risk of oral cancer. Moreover, erythrocyte membrane LI may mediate the association between BMI and oral cancer risk.

In studies conducted in Europe and North America, a lower BMI was observed to be linked to a higher risk of head and neck cancer (HNC) than a normal BMI [25,26,27]. Moreover, being overweight or obese was linked to a lower risk of HNC than being of normal weight [28]. In a pooled analysis by the International HNC Cancer Epidemiology Consortium [25], which included 17 case–control studies, low BMI was associated with a higher risk of HNC, while overweight or obesity was associated with a lower risk. In China, a large-scale study of 921 cases and 806 controls in eight centers investigated the role of BMI on HNC risk. The results showed that overweight and obesity were associated with a lower risk of HNC, while lean individuals had a higher risk of HNC [8]. Similar to previous studies, the current results also showed that BMI was inversely associated with oral cancer risk, and overweight or obesity was associated with a decreased risk of oral cancer.

The mechanism of the effect of BMI on oral cancer remains largely unknown, and one possible explanation is the dysregulation of lipid metabolism. In weight loss individuals, FA metabolism is influenced by weight loss through enzyme activity such as stearoyl-CoA desaturase (SCD) [29]. Obesity is characterized by metabolic disorder which is linked to perturbation of FA balance [30]. It has been reported that obesity tends to be a proinflammatory condition with changes in the FA composition of erythrocyte membranes [31]. A study of normal, overweight, and obese participants found that the FA profile of the erythrocyte membrane was different between the normal weight and obese groups [32]. Obesity associated with lipid accumulation was a major risk for insulin resistance [33], which was closely related to membrane fluidity [34] and FA profile [35].

Alterations in the FA composition of erythrocyte membranes were linked to an increased risk of cancer development [36, 37]. In a prospective study conducted in China, the FA profile of erythrocyte membranes in gastrointestinal cancer patients was found to be significantly different from that in healthy controls [38]. A case–control study within the EPIC Cohort Study discovered that a specific erythrocyte membrane phospholipid FA profile, possibly reflecting both a complicated dietary pattern and altered FA metabolism, was related to progressed colorectal adenoma risk [39]. Mouillot et al. found differences within the FA profile of the erythrocyte membranes of cirrhotic patients with hepatocellular carcinoma and cirrhotic controls without hepatocellular carcinoma [17]. An association between the erythrocyte membrane FA profile and cancer risk has also been found in other cohort studies [40,41,42]. The erythrocyte membrane FA profile differed in the presence of oral cancer. n-3 PUFAs were significantly decreased while oleic acid (C18:1 n-9) were significantly increased in oral cancer patients [43]. In the current study, different distributions of FA profiles in erythrocyte membranes were also observed between oral cancer patients and control participants.

However, the health effects of individual FAs even from the same category were heterogeneous, since the current FA classification was mainly based on structural properties instead of biological properties, such as the affinity between FA molecules and their fluidity. Therefore, a new LI was introduced in the current study and a positive association between LI and oral cancer risk was found. The LI was an overall estimate of the biological properties (melting point) and quantity of FA. LI was created to summarize the concentrations of individual FAs in a record that might be utilized to estimate the profile of FAs in biological samples such as erythrocytes. FA composition could influence skeletal cell membrane function with the aid of altered membrane fluidity, ion permeability, and insulin receptor binding and affinity [44]. A higher LI usually indicates poor membrane permeability and fluidity [13] and may affect the membrane’s physiological function [45]. Fluidity is a critical mediator that links the metabolism and consumption of FAs with cancer risk, and is related to several cellular functions including the properties of certain membrane-bound enzymes, mediator transport, endocytosis, phagocytosis, depolarization-dependent exocytosis, immunology and chemotherapeutic cytotoxicity, prostaglandin production and cell growth [46]. Altered insulin sensitivity and lipotoxicity are mechanisms that may link membrane fluidity to oral cancer risk [22]. Insulin resistance can stimulate cell proliferation and inhibit apoptosis, thus contributing to tumor promotion and metastasis through signal transduction of the insulin-like growth factor-1 receptor pathway [47,48,49]. In addition, the potential influence of lipids on neoplastic development may also be due to their influence on the metabolism of neoplastic cells, the function of lipids as intercellular messengers or as mediators of the inflammatory reaction [50]. Consistent with a previous study, a significant association between erythrocyte membrane FA profile-derived LI and oral cancer risk was observed in our study population.

Given that the FA profile was correlated with both BMI and oral cancer, it is plausible that FA composition could be a causal link between BMI and oral cancer, which was confirmed in the current study with erythrocyte membranes mediating 7% of the association between BMI and oral cancer. Previous studies [51, 52] have reported that obesity affects insulin resistance and consequently is involved in increasing the circulating level of free FA, which affects the tumor immune microenvironment through lipotoxicity. Balaban et al. [53] elucidated the role of FA metabolism in obesity and cancer and indicated that obesity affects tumorigenesis by interfering with the microenvironment of FA metabolism. For example, obesity altered the expression of CD36, which was correlated with FA transport [54] and has been associated with cancer progression. FA-binding proteins have been associated with FA intracellular transport, and obesity may increase the expression of FA-binding proteins and thus play an important role in cancer progression.

Comparisons with other studies

Previous studies have revealed the association between BMI [25,26,27] and cancer as well as the association between changes in the FA profile in the cell membrane and cancer [36, 37]. In addition to findings reported by a previous study, the potential mediating effects of the membrane FA profile on the association of BMI with carcinogenesis were explored. In addition, the LI instead of individual FA levels was used in the current study. LI, which is calculated using both the melting point and level of individual FAs, is a better indicator of membrane functions.

Strength and limitations

The strengths of this study include the following: 1) A comprehensive measurement of the FA profile of erythrocyte membranes was used to explore the relationship between membrane FA composition and oral cancer instead of using individual FA species. 2) Mediation analysis was performed to reveal the potential role of membrane FA on the association of BMI and oral cancer.

There were several limitations of our study. The melting points of several FAs were not available in the LipidBank database and hence could not be considered in the calculation of the LI. However, the levels of these FAs in the membrane were very low at less than 0.5%. Furthermore, this was a hospital-based, single-centre study with a case–control design. Large-scale prospective studies are needed to corroborate our findings.

Conclusion

In summary, the distribution of the FA profile in erythrocyte membranes differed between the oral cancer patients and the control group. LI, which captures the overall lipophilicity of major FAs of erythrocytes, was positively associated with the risk of oral cancer. Associations between BMI and oral cancer risk can be explained, at least in part, by LI. This study revealed the potential role of membrane FA profile in oral cancer and may provide new perspectives in the development of new biomarkers and therapeutic targets for oral cancer.

Availability of data and materials

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Abbreviations

LI:

Lipophilic index

FA:

Fatty acid

BMI:

Body mass index

SFA:

Saturated fatty acid

MUFA:

Monounsaturated fatty acid

PUFA:

Polyunsaturated fatty acid

OR:

Odd ratio

HNC:

Head and neck cancer

SCD:

Stearoyl-CoA desaturase

References

  1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71:209–49.

    Article  PubMed  Google Scholar 

  2. Zhang LW, Li J, Cong X, Hu XS, Li D, Wu LL, et al. Incidence and mortality trends in oral and oropharyngeal cancers in China, 2005-2013. Cancer Epidemiol. 2018;57:120–6.

    Article  PubMed  Google Scholar 

  3. Maasland DH, van den Brandt PA, Kremer B, Goldbohm RA, Schouten LJ. Alcohol consumption, cigarette smoking and the risk of subtypes of head-neck cancer: results from the Netherlands cohort study. BMC Cancer. 2014;14:187–200.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Wang Z, Sturgis EM, Zhang Y, Huang Z, Zhou Q, Wei Q, et al. Combined p53-related genetic variants together with HPV infection increase oral cancer risk. Int J Cancer. 2012;131:E251–8.

    Article  CAS  PubMed  Google Scholar 

  5. Madathil SA, Rousseau MC, Wynant W, Schlecht NF, Netuveli G, Franco EL, et al. Nonlinear association between betel quid chewing and oral cancer: implications for prevention. Oral Oncol. 2016;60:25–31.

    Article  CAS  PubMed  Google Scholar 

  6. Auguste A, Joachim C, Deloumeaux J, Gaete S, Michineau L, Herrmann-Storck C, et al. Head and neck cancer risk factors in the French West Indies. BMC Cancer. 2021;21:1071–9.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Ward HA, Wark PA, Muller DC, Steffen A, Johansson M, Norat T, et al. Measured adiposity in relation to head and neck Cancer risk in the European prospective investigation into Cancer and nutrition. Cancer Epidemiol Biomark Prev. 2017;26:895–904.

    Article  CAS  Google Scholar 

  8. Chen Y, Lee YA, Li S, Li Q, Chen CJ, Hsu WL, et al. Body mass index and the risk of head and neck cancer in the Chinese population. Cancer Epidemiol. 2019;60:208–15.

    Article  PubMed  Google Scholar 

  9. Machate DJ, Figueiredo PS, Marcelino G, Guimaraes RCA, Hiane PA, Bogo D, et al. Fatty acid diets: regulation of gut microbiota composition and obesity and its related metabolic Dysbiosis. Int J Mol Sci. 2020;21:4093–103.

    Article  CAS  PubMed Central  Google Scholar 

  10. Al-Jawadi A, Moussa H, Ramalingam L, Dharmawardhane S, Gollahon L, Gunaratne P, et al. Protective properties of n-3 fatty acids and implications in obesity-associated breast cancer. J Nutr Biochem. 2018;53:1–8.

    Article  CAS  PubMed  Google Scholar 

  11. Balaban S, Shearer R, Lee L, van Geldermalsen M, Schreuder M, Shtein H, et al. Adipocyte lipolysis links obesity to breast cancer growth: adipocyte-derived fatty acids drive breast cancer cell proliferation and migration. Cancer Metab. 2017;5:1–14.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Tulenko TN, Boeze-Battaglia K, Mason RP, Tint GS, Steiner RD, Connor WE, et al. A membrane defect in the pathogenesis of the smith-Lemli-Opitz syndrome. J Lipid Res. 2006;47:134–43.

    Article  CAS  PubMed  Google Scholar 

  13. Kroger J, Jacobs S, Jansen EH, Fritsche A, Boeing H, Schulze MB. Erythrocyte membrane fatty acid fluidity and risk of type 2 diabetes in the EPIC-Potsdam study. Diabetologia. 2015;58:282–9.

    Article  PubMed  Google Scholar 

  14. Aoun M, Corsetto PA, Nugue G, Montorfano G, Ciusani E, Crouzier D, et al. Changes in red blood cell membrane lipid composition: a new perspective into the pathogenesis of PKAN. Mol Genet Metab. 2017;121:180–9.

    Article  CAS  PubMed  Google Scholar 

  15. Amezaga J, Arranz S, Urruticoechea A, Ugartemendia G, Larraioz A, Louka M, et al. Altered red blood cell membrane fatty acid profile in Cancer patients. Nutrients. 2018;10:1853–65.

  16. Pala V, Krogh V, Muti P, Chajes V, Riboli E, Micheli A, et al. Erythrocyte membrane fatty acids and subsequent breast cancer: a prospective Italian study. J Natl Cancer Inst. 2001;93:1088–95.

    Article  CAS  PubMed  Google Scholar 

  17. Mouillot T, Rizk M, Pais de Barros JP, Gilloteau A, Busson A, Bernard-Chabert B, et al. Fatty acid composition of the erythrocyte membrane and risk of hepatocellular carcinoma in cirrhotic patients. Aliment Pharmacol Ther. 2020;52:1503–15.

    CAS  PubMed  Google Scholar 

  18. McGee EE, Kim CH, Wang M, Spiegelman D, Stover DG, Heng YJ, et al. Erythrocyte membrane fatty acids and breast cancer risk by tumor tissue expression of immuno-inflammatory markers and fatty acid synthase: a nested case-control study. Breast Cancer Res. 2020;22:78–90.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Amezaga J, Ugartemendia G, Larraioz A, Bretana N, Iruretagoyena A, Camba J, et al. Altered levels of desaturation and omega-6 fatty acids in breast Cancer Patients' red blood cell membranes. Metabolites. 2020;10:469–81.

  20. Coviello G, Tutino V, Notarnicola M, Caruso MG. Erythrocyte membrane fatty acids profile in colorectal cancer patients: a preliminary study. Anticancer Res. 2014;34:4775–9.

    CAS  PubMed  Google Scholar 

  21. Wu H, Ding EL, Toledo ET, Campos H, Baylin A, Hu FB, et al. A novel fatty acid lipophilic index and risk of CHD in US men: the health professionals follow-up study. Br J Nutr. 2013;110:466–74.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Toledo E, Campos H, Ding EL, Wu H, Hu FB, Sun Q, et al. A novel fatty acid profile index--the lipophilic index--and risk of myocardial infarction. Am J Epidemiol. 2013;178:392–400.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Chen Q, Wang J, Wang J, Lin J, Chen L, Lin LS, et al. Erythrocyte omega-3 polyunsaturated fatty acids are inversely associated with the risk of oral cancer: a case-control study. Nutr Diabetes. 2020;10:35–42.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Ainsworth BE, Haskell WL, Herrmann SD, Meckes N, Bassett DR Jr, Tudor-Locke C, et al. 2011 compendium of physical activities: a second update of codes and MET values. Med Sci Sports Exerc. 2011;43:1575–81.

    Article  PubMed  Google Scholar 

  25. Gaudet MM, Olshan AF, Chuang SC, Berthiller J, Zhang ZF, Lissowska J, et al. Body mass index and risk of head and neck cancer in a pooled analysis of case-control studies in the international head and neck Cancer epidemiology (INHANCE) consortium. Int J Epidemiol. 2010;39:1091–102.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Gaudet MM, Kitahara CM, Newton CC, Bernstein L, Reynolds P, Weiderpass E, et al. Anthropometry and head and neck cancer:a pooled analysis of cohort data. Int J Epidemiol. 2015;44:673–81.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Park SL, Lee YC, Marron M, Agudo A, Ahrens W, Barzan L, et al. The association between change in body mass index and upper aerodigestive tract cancers in the ARCAGE project: multicenter case-control study. Int J Cancer. 2011;128:1449–61.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Petrick JL, Gaudet MM, Weissler MC, Funkhouser WK, Olshan AF. Body mass index and risk of head and neck cancer by race: the Carolina head and neck Cancer epidemiology study. Ann Epidemiol. 2014;24:160–4.

    Article  PubMed  Google Scholar 

  29. Swenne I, Rosling A, Tengblad S, Vessby B. Essential fatty acid status in teenage girls with eating disorders and weight loss. Acta Paediatr. 2011;100:762–7.

    Article  CAS  PubMed  Google Scholar 

  30. Andersen MK, Jorsboe E, Sandholt CH, Grarup N, Jorgensen ME, Faergeman NJ, et al. Identification of novel genetic determinants of erythrocyte membrane fatty acid composition among Greenlanders. PLoS Genet. 2016;12:e1006119.

    Article  PubMed  PubMed Central  Google Scholar 

  31. Jauregibeitia I, Portune K, Rica I, Tueros I, Velasco O, Grau G, et al. Potential of erythrocyte membrane lipid profile as a novel inflammatory biomarker to distinguish metabolically healthy obesity in children. J Pers Med. 2021;11:337–48.

  32. Shen J, Li J, Hua Y, Ding B, Zhou C, Yu H, et al. Association between the erythrocyte membrane fatty acid profile and cognitive function in the overweight and obese population aged from 45 to 75 years old. Nutrients. 2022;14:914–30.

  33. Kojta I, Chacińska M, Błachnio-Zabielska A. Obesity, bioactive lipids, and adipose tissue inflammation in insulin resistance. Nutrients. 2020;12:1305–23.

  34. Vázquez C, Rovira N, Ruiz-Gutiérrez V, Planas J. Developmental changes in glucose transport, lipid composition, and fluidity of jejunal BBM. Am J Phys. 1997;273:R1086–93.

    Google Scholar 

  35. Baur L, O'Connor J, Pan D, Storlien L. Relationships between maternal risk of insulin resistance and the child's muscle membrane fatty acid composition. Diabetes. 1999;48:112–6.

    Article  CAS  PubMed  Google Scholar 

  36. Currie E, Schulze A, Zechner R, Walther TC, Farese RV Jr. Cellular fatty acid metabolism and cancer. Cell Metab. 2013;18:153–61.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Kuriki K, Wakai K, Hirose K, Matsuo K, Ito H, Suzuki T, et al. Risk of colorectal cancer is linked to erythrocyte compositions of fatty acids as biomarkers for dietary intakes of fish, fat, and fatty acids. Cancer Epidemiol Biomark Prev. 2006;15:1791–8.

    Article  CAS  Google Scholar 

  38. Lin S, Li T, Liu X, Wei S, Liu Z, Hu S, et al. Abnormal octadeca-carbon fatty acids distribution in erythrocyte membrane phospholipids of patients with gastrointestinal tumor. Medicine (Baltimore). 2017;96:e7189–94.

    Article  CAS  Google Scholar 

  39. Cottet V, Collin M, Gross AS, Boutron-Ruault MC, Morois S, Clavel-Chapelon F, et al. Erythrocyte membrane phospholipid fatty acid concentrations and risk of colorectal adenomas: a case-control nested in the French E3N-EPIC cohort study. Cancer Epidemiol Biomark Prev. 2013;22:1417–27.

    Article  CAS  Google Scholar 

  40. Chajes V, Joulin V, Clavel-Chapelon F. The fatty acid desaturation index of blood lipids, as a biomarker of hepatic stearoyl-CoA desaturase expression, is a predictive factor of breast cancer risk. Curr Opin Lipidol. 2011;22:6–10.

    Article  CAS  PubMed  Google Scholar 

  41. Rifkin SB, Shrubsole MJ, Cai Q, Smalley WE, Ness RM, Swift LL, et al. PUFA levels in erythrocyte membrane phospholipids are differentially associated with colorectal adenoma risk. Br J Nutr. 2017;117:1615–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Straka S, Lester JL, Cole RM, Andridge RR, Puchala S, Rose AM, et al. Incorporation of eicosapentaenioic and docosahexaenoic acids into breast adipose tissue of women at high risk of breast cancer: a randomized clinical trial of dietary fish and n-3 fatty acid capsules. Mol Nutr Food Res. 2015;59:1780–90.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Maulucci G, Cohen O, Daniel B, Sansone A, Petropoulou PI, Filou S, et al. Fatty acid-related modulations of membrane fluidity in cells: detection and implications. Free Radic Res. 2016;50:S40–50.

    Article  CAS  PubMed  Google Scholar 

  44. Patel PS, Sharp SJ, Jansen E, Luben RN, Khaw KT, Wareham NJ, et al. Fatty acids measured in plasma and erythrocyte-membrane phospholipids and derived by food-frequency questionnaire and the risk of new-onset type 2 diabetes: a pilot study in the European prospective investigation into Cancer and nutrition (EPIC)-Norfolk cohort. Am J Clin Nutr. 2010;92:1214–22.

    Article  CAS  PubMed  Google Scholar 

  45. van Meer G, Voelker DR, Feigenson GW. Membrane lipids: where they are and how they behave. Nat Rev Mol Cell Biol. 2008;9:112–24.

    Article  PubMed  PubMed Central  Google Scholar 

  46. Spector AA, Yorek MA. Membrane lipid composition and cellular function. J Lipid Res. 1985;26:1015–35.

    Article  CAS  PubMed  Google Scholar 

  47. Hua H, Kong Q, Yin J, Zhang J, Jiang Y. Insulin-like growth factor receptor signaling in tumorigenesis and drug resistance: a challenge for cancer therapy. J Hematol Oncol. 2020;13:64–80.

    Article  PubMed  PubMed Central  Google Scholar 

  48. Gapstur SM, Gann PH, Lowe W, Liu K, Colangelo L, Dyer A. Abnormal glucose metabolism and pancreatic cancer mortality. JAMA. 2000;283:2552–8.

    Article  CAS  PubMed  Google Scholar 

  49. Sun W, Lu J, Wu S, Bi Y, Mu Y, Zhao J, et al. Association of insulin resistance with breast, ovarian, endometrial and cervical cancers in non-diabetic women. Am J Cancer Res. 2016;6:2334–44.

    CAS  PubMed  PubMed Central  Google Scholar 

  50. Jurczyszyn A, Czepiel J, Gdula-Argasinska J, Czapkiewicz A, Biesiada G, Drozdz M, et al. Erythrocyte membrane fatty acids in multiple myeloma patients. Leuk Res. 2014;38:1260–5.

    Article  CAS  PubMed  Google Scholar 

  51. de Jong A, Kloppenburg M, Toes R, Ioan-Facsinay A. Fatty acids, lipid mediators, and T-cell function. Front Immunol. 2014;5:483–90.

    PubMed  PubMed Central  Google Scholar 

  52. Zhang Y, Rao E, Zeng J, Hao J, Sun Y, Liu S, et al. Adipose fatty acid binding protein promotes saturated fatty acid-induced macrophage cell death through enhancing Ceramide production. J Immunol. 2017;198:798–807.

    Article  CAS  PubMed  Google Scholar 

  53. Balaban S, Lee LS, Schreuder M, Hoy AJ. Obesity and cancer progression: is there a role of fatty acid metabolism? Biomed Res Int. 2015;2015:274585.

    Article  PubMed  PubMed Central  Google Scholar 

  54. Bechmann LP, Gieseler RK, Sowa JP, Kahraman A, Erhard J, Wedemeyer I, et al. Apoptosis is associated with CD36/fatty acid translocase upregulation in non-alcoholic steatohepatitis. Liver Int. 2010;30:850–9.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors appreciate study participants that contributed to this study.

Funding

This study was supported by the Fujian Natural Science Foundation Program (grant numbers: 2020 J01639), grant of Science and Technology of Fujian-Province (2019 L3006), and grant of Science and Technology of Fujian Province (No. 2020 L3009).

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Authors and Affiliations

  1. Department of Epidemiology and Health Statistics, Fujian Provincial Key Laboratory of Environment Factors and Cancer, School of Public Health, Fujian Medical University, 1 Xueyuan Road, Fuzhou, 350122, China

    Yi Fan, Yaping Wang, Jing Wang, Yanni Li, Sijie Wang, Yanfeng Weng, Qiujiao Yang, Chen Chen, Fa Chen, Baochang He & Fengqiong Liu

  2. Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Fujian Key Laboratory of Tumor Microbiology, Fujian Medical University, Fujian, China

    Yi Fan, Yaping Wang, Jing Wang, Yanni Li, Sijie Wang, Yanfeng Weng, Qiujiao Yang, Chen Chen, Fa Chen, Baochang He & Fengqiong Liu

  3. School of Public Health, Zunyi Medical University, Zunyi, Guizhou, China

    Qing Chen

  4. Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Fujian Medical University, Fujian, China

    Lisong Lin & Yu Qiu

  5. Laboratory Center, The Major Subject of Environment and Health of Fujian Key Universities, School of Public Health, Fujian Medical University, Fujian, China

    Jing Wang

Authors
  1. Yi Fan
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  2. Qing Chen
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  3. Yaping Wang
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  4. Jing Wang
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  8. Qiujiao Yang
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  9. Chen Chen
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  10. Lisong Lin
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  11. Yu Qiu
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  12. Fa Chen
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  13. Jing Wang
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  14. Baochang He
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  15. Fengqiong Liu
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Contributions

The authors’ responsibilities were as follows –Yi Fan: wrote the manuscript and analyzed the data; Qing Chen, Jing Wang: performed research and completed the experiment; Yaping Wang: assisted with the data analysis; Jing Wang, Sijie Wang, Yanni Li, Qiujiao Yang, Chen Chen: involved in data collection; Lisong Lin, Yu Qiu: coordinated the data and blood samples collection of the hospital; Baochang He, Fa Chen: assisted with the interpretation, and critically reviewed drafts of the paper; Fengqiong Liu: revised the manuscript and put forward the ideas of study; and all authors: read and approved the final manuscript.

Corresponding authors

Correspondence to Baochang He or Fengqiong Liu.

Ethics declarations

Ethics approval and consent to participate

The study protocol was accepted by Fujian Medical University’s Ethics Committee (Approval number:2011053; Approval date: March 10, 2011) and followed the Declaration of Helsinki’s ethical standards. All of the participants gave their informed permission. The demographic data were obtained by face-to-face interviews using a standardized questionnaire.

Competing interests

The authors declare that they have no conflict of interest.

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Supplementary Information

Additional file 1: Supplement Figure 1.

Association between the lipophilic index and oral cancer by stratified analysis.

Additional file 2: Supplement Table 1.

Multivariable generalized Linear Models of mediation of outcome models.

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Fan, Y., Chen, Q., Wang, Y. et al. Mediation analysis of erythrocyte lipophilic index on the association between BMI and risk of oral cancer. Lipids Health Dis 21, 96 (2022). https://doi.org/10.1186/s12944-022-01704-z

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  • DOI: https://doi.org/10.1186/s12944-022-01704-z

Keywords

  • Fatty acids
  • Membrane fluidity
  • Lipophilic index
  • Oral cancer
  • Mediation analysis

Lipids in Health and Disease

ISSN: 1476-511X