- Open Access
Fatty acid distribution of cord and maternal blood in human pregnancy: special focus on individual trans fatty acids and conjugated linoleic acids
Lipids in Health and Disease volume 10, Article number: 247 (2011)
Maternal nutrition in pregnancy has a crucial impact on the development of the fetus. Dietary trans fatty acids (t FA) are known to have adverse health effects, especially during pregnancy. However, the distribution of t FA produced via partial hydrogenation of vegetable oils (mainly elaidic acid; t 9) differs compared to ruminant-derived t FA (mainly vaccenic acid; t 11). Recent findings indicate that they may have different impact on human health.
Therefore, in this study, plasma and erythrocytes of mother-child pairs (n = 55) were sampled to investigate the distribution of t FA, including individual trans C18:1 fatty acids and conjugated linoleic acids (CLA) in fetal related to maternal lipids; with additional consideration of maternal dairy fat intake.
Portion of t 9 and t 11, but also of c 9,t 11 CLA was higher in maternal than in fetal blood lipids. The portion of t 9 in maternal and fetal lipids differed only slightly. In contrast, the portion of fetal t 11 was only half of that in maternal blood. This led to a fetal t 9/t 11-index in plasma and erythrocytes being twice as high compared to the maternal values. A high dairy fat intake resulted in elevated portions of t 11 and its Δ9-desaturation product c 9,t 11 CLA in maternal blood. In contrast, in the respective fetal blood lipids only c 9,t 11 CLA, but not t 11 was increased. Nevertheless, a positive association between maternal and fetal plasma exists for both t 11 and c 9,t 11 CLA. Furthermore, in contrast to t 9, t 11 was not negatively associated with n-3 LC-PUFA in fetal blood lipids.
Fetal blood fatty acid composition essentially depends on and is altered by the maternal fatty acid supply. However, in addition to dietary factors, other aspects also contribute to the individual fatty acid distribution (oxidation, conversion, incorporation). The lower portion of fetal t 11 compared to maternal t 11, possibly results from Δ9-desaturation to c 9,t 11 CLA and/or oxidation. Based on the fatty acid distribution, it can be concluded that t 11 differs from t 9 regarding its metabolism and their impact on fetal LC-PUFA.
Maternal nutrition may have a crucial impact on the development of the fetus during pregnancy and even for the child after birth . Long chain polyunsaturated fatty acids (LC-PUFA), such as C20:4 n-6 (arachidonic acid; AA) and C22:6 n-3 (docosahexaenoic acid; DHA) are known to be essential for fetal development [2–4]. Moreover, n-3 LC-PUFA have been shown to modulate the fetal immune system and, thus, possibly decrease the risk for later atopy development . In contrast, trans fatty acids (t FA) are considered to enhance the risk of coronary heart disease [6, 7], atopy , complications in pregnancy, e.g. preeclampsia  and to impair fetal development . Extensive data on preferred LC-PUFA transport from mother to fetus [11, 12] as well as higher LC-PUFA concentrations in fetal than in maternal blood [13–16] have been published over the last few decades. In contrast, data of fetal blood concentrations and profile of conjugated linoleic acids (CLA) and t FA are scarce. Moreover, in results from studies concerning t FA, only mean percentage values of total t FA in maternal and/or fetal plasma and erythrocyte lipids have been reported [13, 17–20].
However, since the amount and distribution of individual t FA, especially trans C18:1, differs considerably between industrial and natural (ruminant) t FA sources, a discussion has arisen regarding the extent to which ruminant t FA raises the risk for cardiovascular diseases in comparison to industrial t FA. The latter mainly comprises of trans- 9 C18:1 (elaidic acid; t 9) and trans- 10 C18:1 . In contrast, trans- 11 C18:1 (vaccenic acid; t 11) is the major trans C18:1 in ruminant t FA principally resulting in a low t 9/t 11-index in dairy products (< 1) . The t 11 in dairy fat derives from microbial biohydrogenation of PUFA in the rumen. Dairy fat also contains CLA, especially c 9,t 11 CLA produced by rumen microbes and primarily by endogenous Δ9-desaturation of t 11 by the mammary gland .
It has been argued that in contrast to other trans C18:1, intake of t 11 is not associated with coronary heart disease or inflammation . In fact, t 11 may even impart health benefits due to its function as a metabolic precursor of c 9,t 11 CLA. This conversion also occurs in humans [22, 25, 26]. Both, t 11 and c 9,t 11 CLA have been shown to exert anti-inflammatory effects in allergy models [27, 28], and t 11 did not negatively alter inflammatory parameters in humans . High maternal dairy fat intake led to elevated c 9,t 11 CLA and t 11, thus decreasing the t 9/t 11-index in breast milk . These ruminant fatty acids in breast milk showed protective effects against the development of atopic manifestation in infants .
To date, no corresponding data concerning individual trans C18:1 such as t 9 and t 11 are available for maternal and the corresponding fetal blood. This study aimed at characterising the distribution of individual trans isomers of maternal and fetal blood lipids and their association with LC-PUFA in fetal blood lipids. In addition, the relationship between maternal intake of dairy products and the maternal and fetal blood fatty acid profile was investigated.
Subjects and study design
Blood samples from 55 mothers together with cord blood from their neonates were collected at birth. In this study, only healthy infants born at term after uncomplicated pregnancies were included. Mothers with gestational diabetes or those taking pharmaceuticals were excluded. After birth, mothers were requested to answer two questionnaires: one focussing on obstetric history, lifestyle, and socioeconomic factors and the other was a food frequency questionnaire (FFQ) dealing with foods from a variety of groups, such as dairy products, fish, oils, seeds or nuts. The study protocol was approved by the local ethics committee (Nr. 1345-06/04). Informed written consent was obtained from all mothers.
Sample collection and preparation for gas chromatography
Maternal blood was drawn at birth from a peripheral vein. Fetal blood was drawn by venipuncture from the placental portion of the umbilical cord immediately after clamping. Blood was collected in EDTA-tubes (S-Monovette 9 ml KE3, Sarstedt). Plasma was separated by centrifugation (1500 × g, 10 min, 4°C) and erythrocytes were washed three times with physiological sodium chloride solution. Plasma and erythrocytes were frozen immediately at -80°C and stored until lipid extraction.
Lipid extraction and transmethylation
Lipids were extracted from plasma and erythrocytes based on the method of Bligh and Dyer . Lipid extracts were transmethylated by using a combination of 0.5 N methanolic sodium hydroxide (Merck) and 10% (w/w, Supelco) boron trifluoride-methanol (100°C for 5 min each). Subsequently, fatty acid methyl esters (FAME) were purified by thin layer chromatography and dissolved in n-hexane for analysis . A system of two GC/FID methods was used to analyse the full fatty acid spectrum from C4 to C26 including CLA (GC-17 V3 Shimadzu; DB-225MS: 60 m, i.d. 0.25 mm, 0.25 μm film thickness; Agilent Technologies) as well as cis and trans isomers of C18:1, trans C18:2 and C18:3 (GC-2010, Shimadzu; CP-select 200 m × 0.25 mm i.d. with 0.25 μm film thickness; Varian) . In brief, injector and detector temperatures were maintained at 260°C and 270°C, respectively, with hydrogen as carrier gas . In total 88 fatty acids were identified and detectable trans C18:1 comprise the following isomers: t 4, t 5, t 6/7/8, t 9, t 10, t 11, t 12, t 13/14, t 15, and t 16 C18:1. Furthermore, t 9,t 12; c 9,t 12; t 9,c 12 C18:2; t 3,c 9,c 11 and c 8,t 10,t 12 C18:3, were summarised as total t FA. Fatty acid concentrations were expressed as the percentage of the total area of all FA peaks (% of total FAME).
Data evaluation and statistics
Evaluation of food frequency questionnaires (n = 41) was carried out using Prodi 5.5 Nutriscience software (summarized in Table 1). Fourteen questionnaires had to be excluded due to unreliable data.
Statistical analysis was performed via PASW statistics, version 17 (SPSS Inc.). To evaluate differences and correlations between maternal and fetal fatty acid compositions, paired student's t-test was conducted and Pearson correlation coefficient was calculated. Unpaired t-test was used to determine the difference of means in the subgroups of high and low dairy fat intake. Data were reported as means ± SD. Significance was defined as P ≤ 0.05.
The mean maternal age at birth was 29.2 years. Infants had normal birth weight and length (Table 2).
Mothers reported that they did not essentially change their dietary habits during pregnancy. In general, they consumed western-style diets that were predominantly omnivore. The fat intake (n = 41) averaged 124 g per day which corresponds to 45% of total energy intake (en%), with high inter-individual variations (from 55 to 215 g/d; Table 1). Mean dairy fat intake was 36.7 g/d, ranging from 7.29 g/d (2.2 en%) to 82.1 g/d (39.3 en%). Mothers who reported a high dairy fat intake (> 40 g/d) tended to have higher energy uptake due to the raised dietary fat (increased by 1.2 times). In addition, their dietary intake of saturated and short chain fatty acids was also 1.3 to 2 times higher (Table 1). Overall, fish was reportedly consumed less than once a week and n-3 PUFA supplementation (e.g. by fish oil capsules) was not common (n = 11; 20%). A high dairy fat intake was not associated with fish oil supplementation and increased intake of n-3 PUFA-rich foods (e.g., fish, nuts, and vegetable oils). If fish oil was supplemented, a higher proportion of n-3 LC-PUFA in maternal lipids was not observed (data not shown).
Fatty acid profile in maternal and fetal blood lipids (n = 55)
- Trans fatty acids
The mean total t FA, as well as t 9 and t 11 in plasma and erythrocyte lipids were significantly higher in maternal compared to fetal lipids (Table 3). However, in both fetal lipids fractions, t 11 levels were half of t 9 values leading to a higher fetal t 9/t 11-index compared to the maternal index in both plasma and erythrocytes (Table 3).
Total t FA in maternal plasma (Pmat) correlated positively with total t FA concentrations in fetal plasma (Pfet). There was no correlation of t 9 between Pmat and Pfet. On the contrary, there was a positive correlation between Pmat and Pfet for t 11. In general, no correlation for total t FA as well as t 9 and t 11 was found between maternal and fetal erythrocytes (Emat, Efet, respectively; Table 3).
On the other hand, positive correlations were found within the respective blood fraction between t 9 and t 11 (Pmat: r = 0.33, P < 0.05; Pfet: r = 0.39, P < 0.001; Emat: r = 0.12, P = 0.37; Efet: r = 0.89, P < 0.001; data not shown).
In general, in most samples, proportions of t 4 and t 5 C18:1 as well as of t 3,c 9,c 11 C18:3 were below the detection limit. The co-eluting isomers t 13 and t 14 C18:1 were the only trans isomers which were lower in maternal than in fetal lipids (Table 3). [However, since there was no pre-separation of cis C18:1 via Ag+-TLC before GC analysis, an overestimation of t 13/14 C18:1 due to co-elution with cis C18:1 isomers c 6-8 could have occurred . It is also possible that c 6-8 C18:1 are especially relevant in fetal lipids, however, no data are available in the literature].
- Conjugated linoleic acids
The c 9,t 11 CLA was significantly higher in maternal than in fetal lipids (Table 3). Moreover, positive correlations of c 9,t 11 CLA between maternal and fetal lipids were stronger for plasma than for erythrocytes (Table 3).
In Pmat and Pfet, elevated c 9,t 11 CLA were seen compared to t 11, whilst both were equally distributed in the respective erythrocyte lipids. In addition, maternal t 11 in plasma and erythrocytes was positively correlated to the respective fetal c 9,t 11 CLA (r = 0.51, r = 0.59; P < 0.001, respectively, data not shown).
- Polyunsaturated fatty acids
Quantities of AA, n-6, and DHA, n-3, were significantly higher in Pfet and Efet than in the respective maternal lipids. In contrast, proportions of linoleic acid (C18:2 n-6) were higher in the maternal than in fetal lipids (Table 3).
- Correlation between trans fatty acids and polyunsaturated fatty acids
Analysis of correlations between t FA and LC-PUFA revealed a heterogeneous result. However, a significant negative association was found in Pfet between t 9 and n-3 LC-PUFA (total, DPA n-3, DHA) while t 11 was negatively associated with n-6 LC-PUFA in Emat and Efet (Table 4).
Furthermore, regarding correlations of maternal to fetal fatty acids, t 11 in maternal blood lipids was positively associated with fetal n-3 LC-PUFA, especially in erythrocytes (Table 4(B)).
Association between dairy fat intake and fatty acid profile of blood lipids (n = 41)
A high maternal intake of dairy fat (> 40 g/d; n = 14) resulted in an elevated amount of milk specific fatty acids such as C15:0, C17:0 and t 11 in both maternal lipid fractions (Pmat and Emat) compared to mothers with lower dairy fat intake (n = 27; Table 5). In contrast, in fetal lipids, the high maternal dairy fat intake was only reflected by a higher c 9,t 11 CLA in plasma and erythrocytes, whereas t 11 was not elevated. But, since t 11 was elevated in maternal lipids, the t 9/t 11-index had decreased, which was also shown in fetal lipids. In addition, due to a high dairy fat intake, n-3 LC-PUFA such as EPA, DPA, and DHA were elevated in fetal lipids, particularly in plasma (Table 5).
Data analysis regarding fatty acid correlation between maternal and fetal lipids clearly showed that both t 11 and c 9,t 11 CLA were positively correlated between Pmat and Pfet in the case of low and high dairy fat intake (Figure 1). In contrast, no correlation was observed for t 9 between maternal and fetal lipids, independently of dairy fat intake (Figure 1). A high dairy fat intake did not result in a negative correlation between t 11 and t 9 in all maternal and fetal blood lipids (data not shown).
The quantities of fatty acids in maternal and, especially, in fetal blood are under the influence of various factors. Since t FA are not synthesised in human tissue, all t FA with isolated double bonds present in fetal blood lipids can only be due to a transplacental transfer and must originate from maternal diet. Herein, we investigated the fatty acid composition of plasma and erythrocytes in mother-child pairs at birth, focussing on total t FA and individual trans C18:1 such as t 9 and t 11 with special regard to dairy fat intake.
-Total trans fatty acids in maternal and fetal blood lipids
In the present study, total t FA concentrations in maternal and fetal lipids were about 0.5 to 0.8% of FAME, with generally higher levels in maternal and plasma lipids compared to fetal and erythrocyte lipids, respectively. In several recently published European studies, values for total t FA in maternal and fetal erythrocytes and plasma lipids range from 0.08% to 0.45% in phospholipids and up to 2.74% in the other lipid fractions, however, with higher t FA values in maternal than in fetal lipids [13, 19, 20, 35]. Nevertheless, comparison of data for total t FA is difficult since percentages of single fatty acids may vary according to the analysed plasma fractions (phospholipids vs. total lipids; ) and the applied method of analysis (GC-column, varying numbers of analysed/identified fatty acids, integration parameters, management of un-identified peaks).
- trans fatty acid intake
Following a number of accounts regarding adverse health effects [6, 37], the t FA, content in foods has continuously decreased over the last decades [21, 38]. Recent reports estimate the mean dietary t FA intake in Germany at 2.3 g/d for men (0.8 en%) and 1.6 g/d for women (0.74 en%; ). Since t FA content varies strongly within food categories , the exact intake of t FA is difficult to calculate. Furthermore, the distribution of individual t FA isomers varies according to their origin. In dairy fat, t 11 is generally the major t 18:1 containing about 1.0 to 2.0% of FAME [21, 40]. Thus, the present maternal mean values for low and high dairy fat intake were approximately 27.1 and 56.3 g/d, which were estimated as corresponding to about 0.3 to 0.6 g t 11/d, respectively (Table 2). This assessment is in line with recent data regarding the average t 11 intake . In contrast, the t 9 intake in the age group 30 ± 5 years of the present study population was found to be about twice as high as the t 11 intake  resulting in a mean dietary t 9/t 11-index of about 2.
-Individual trans fatty acids in blood lipids
Hardly any data are available in the literature concerning individual trans C18:1 such as t 11 and t 9 in maternal and/or fetal blood lipids. The present proportions of t 9 and t 11 were similar in maternal blood lipids (Pmat 0.12 vs. 0.10). Whereas t 9 in fetal blood lipids tended to be lower compared to maternal lipids, t 11 was only half the value of maternal t 11 (Pmat 0.10 vs. Pfet 0.05; Table 3). This resulted in fetal t 9/t 11-indices being twice as high as maternal indices (Table 3, 5).
A general positive correlation of t 11, but not of t 9 was seen between maternal and fetal plasma (Table 3 Figure 1). However, fetal t 11 was about the half that of t 9 (Table 3). This low value could indicate differences in materno-fetal transfer as well as metabolism (oxidation, conversion, incorporation) of both individual trans isomers t 9 and t 11, as has been observed in rat hepatocytes [41, 42].
-Conjugated linoleic acids in the diet and blood lipids
Dairy fat contains CLA (mainly c 9,t 11 CLA), which is formed by Δ9-desaturation of t 11 . In dairy fat and human breast milk, t 11 was generally higher compared to c 9,t 11 CLA, whilst the ratio ranged from 4:1 to 2:1 [31, 43, 44].
In the present study, irrespective of dairy fat intake, t 11 was lower compared to c 9,t 11 CLA in lipids of maternal and fetal plasma (1:2; Table 3) probably reflecting the conversion of t 11 to c 9,t 11 CLA by Δ9-desaturase and/or its preferred oxidation . In contrast, the ratio of t 11 to c 9,t 11 CLA in erythrocyte lipids was about 1:1, since maternal and fetal c 9,t 11 CLA was lower compared to their levels in plasma, in accordance with data described by Mueller et al . This aspect may be caused by a higher incorporation of CLA into neutral lipids than into phospholipids, the major lipid fraction in erythrocytes . Further, our results confirmed that maternal c 9,t 11 CLA (dietary and of endogenous origin) was positively correlated to fetal c 9,t 11 CLA (Table 3) .
-Impact of high dairy fat intake on composition of fatty acids in blood lipids
The present results clearly showed an association between ruminant fat consumption and fatty acid distribution of human lipids. A high dairy fat intake in mothers (> 40 g/d) resulted in increased c 9,t 11 CLA and total t FA, especially t 11 in blood lipids. Moreover, the raised t 11 concentrations resulted in a decreased maternal t 9/t 11-index (Table 5).
In breast milk of mothers with comparably high consumption of dairy fat (> 40 g/d), elevated t 11 and c 9,t 11 CLA and a decreased t 9/t 11-index were also observed [30, 44]. The increase in the milk specific fatty acids C15:0 and C17:0 in maternal blood also confirmed the high dairy fat intake in the present study (Table 5). Similar results were obtained in breast milk in a former study . Interestingly, due to a maternal high dairy fat intake, only c 9,t 11 CLA and not t 11 was elevated in the corresponding fetal blood lipids (Table 5).
However, there was a positive correlation of t 11 in plasma between mother and child (not in erythrocytes, Figure 1). The t 9 levels did not significantly differ and there was no correlation between maternal and fetal lipids on comparing a high and low dairy fat diet (Table 5). Although the results point to differences between t 9 and t 11 regarding metabolism and/or transfer, respective data are not yet available for humans.
- Trans fatty acids and long chain-PUFA in maternal and fetal blood lipids
The composition of maternal dietary fat and body fat depot [14, 35] strongly accounts for the fatty acid composition in fetal tissue. However, the increased amounts of n-6 LC-PUFA (AA, DPA) and n-3 LC-PUFA (DPA, DHA) in fetal plasma and erythrocytes compared to maternal lipids observed in the present and former studies is physiologically consistent and attributed to the special requirements of the fetus (Table 3) [46, 47]. Further, a wide range of fatty acid transporters and binding proteins in the placenta determines the enrichment of these LC-PUFA [11, 12].
Several publications show an inverse association between total t FA and both n-6 (e.g. AA) as well as n-3 LC-PUFA (e.g. DHA) in blood lipids. However, since these data only deal with either maternal or fetal blood lipids and not with their interaction as well as different lipid fractions were used, comparison is difficult [18, 20]. In the present study, total t FA were also inversely associated with the sum of n-6, but not n-3 LC-PUFA within the same blood fraction (partly significant; Table 4(A)). Regarding n-3 LC-PUFA in fetal plasma, a negative correlation was only found for t 9, but not for t 11 (Table 4(A). This result might be of relevance, since n-3 have been shown to extend anti-inflammatory effects .
Concerning the relationship between maternal and fetal lipids, the current study data showed a positive correlation of t 11 to n-3 LC-PUFA, however, the result was only significant in erythrocytes (Table 4(B)). This effect could be associated with a higher dairy fat intake, since n-3 LC-PUFA (EPA, DPA, DHA) were significantly elevated in the respective fetal plasma (Table 5). Some studies have revealed the ability of t FA to inhibit several enzymes involved in LC-PUFA synthesis but without distinguishing between industrial and ruminant t FA [48, 49]. However, there is little data regarding the impact of t FA on placental fatty acid transporters showing that t 9 may inhibit binding of PUFA at placental membranes .
Differences between t 9 and t 11 indicated herein might be the result of isomer-specific influences on transcription factors (such as PPARs) involved in the expression of placental transport proteins [51, 52]. From cellular and animal models, there is evidence that t 9 inhibits  and t 11 activates  PPAR expression. Thus, differences in PPAR activation by t 9 and t 11 could interfere with the cellular uptake of LC-PUFA into the placenta [55, 56].
We analysed for the first time individual isomers of trans C18:1 in blood lipids of mother-child pairs at birth and found differences between t 9 and t 11.
In fetal lipids, t 11 accumulation was only half that of t 9, probably due to t 11 conversion to c 9,t 11 CLA. In general, total t FA, including t 9 and t 11, were higher in maternal than in fetal lipids. The present results also demonstrated that maternal high dairy fat intake led to increased t 11 and c 9,t 11 CLA in the blood lipids. However, t 11 was not increased in the respective fetal lipids. In addition, the essential n-3 LC-PUFA were elevated in fetal blood lipids.
Thus, t FA have to be distinguished according to their origin (ruminant or industrial) to separately investigate their possible influence on fetal development and human health.
conjugated linoleic acids
- Emat and Efet:
maternal and fetal erythrocytes
fatty acid methyl esters
monounsaturated fatty acids
- n-3 and n-6 LC-PUFA:
omega-3 and omega-6 long chain polyunsaturated fatty acids
- Pmat and Pfet:
maternal and fetal plasma
saturated fatty acids
- t FA trans:
- t 9 trans:
-9 C18:1 (elaidic acid)
- t 11 trans:
-11 C18:1 (vaccenic acid).
Barker DJ: The origins of the developmental origins theory. J Intern Med. 2007, 261: 412-417. 10.1111/j.1365-2796.2007.01809.x
Warner JO: The early life origins of asthma and related allergic disorders. Arch Dis Child. 2004, 89: 97-102. 10.1136/adc.2002.013029
Wyrwoll CS, Mark PJ, Mori TA, Puddey IB, Waddell BJ: Prevention of programmed hyperleptinemia and hypertension by postnatal dietary omega-3 fatty acids. Endocrinology. 2006, 147: 599-606.
Enke U, Seyfarth L, Schleussner E, Markert UR: Impact of PUFA on early immune and fetal development. Br J Nutr. 2008, 100: 1158-1168. 10.1017/S000711450801413X
Prescott SL, Barden AE, Mori TA, Dunstan JA: Maternal fish oil supplementation in pregnancy modifies neonatal leukotriene production by cord-blood-derived neutrophils. Clin Sci (Lond). 2007, 113: 409-416. 10.1042/CS20070111.
Mozaffarian D, Aro A, Willett WC: Health effects of trans-fatty acids: experimental and observational evidence. Eur J Clin Nutr. 2009, 63 (Suppl 2): S5-21.
van de Vijver LP, van Poppel G, van Houwelingen A, Kruyssen DA, Hornstra G: Trans unsaturated fatty acids in plasma phospholipids and coronary heart disease: a case-control study. Atherosclerosis. 1996, 126: 155-161. 10.1016/0021-9150(96)05906-0
Weiland SK, von Mutius E, Husing A, Asher MI: Intake of trans fatty acids and prevalence of childhood asthma and allergies in Europe. ISAAC Steering Committee. Lancet. 1999, 353: 2040-2041. 10.1016/S0140-6736(99)01609-8
Williams MA, King IB, Sorensen TK, Zingheim RW, Troyer BL, Zebelman AM, Luthy DA: Risk of preeclampsia in relation to elaidic acid (trans fatty acid) in maternal erythrocytes. Gynecol Obstet Invest. 1998, 46: 84-87. 10.1159/000010007
Innis SM: Trans fatty intakes during pregnancy, infancy and early childhood. Atheroscler Suppl. 2006, 7: 17-20.
Haggarty P, Ashton J, Joynson M, Abramovich DR, Page K: Effect of maternal polyunsaturated fatty acid concentration on transport by the human placenta. Biol Neonate. 1999, 75: 350-359. 10.1159/000014115
Dutta-Roy AK: Cellular uptake of long-chain fatty acids: role of membrane-associated fatty-acid-binding/transport proteins. Cell Mol Life Sci. 2000, 57: 1360-1372. 10.1007/PL00000621
Vlaardingerbroek H, Hornstra G: Essential fatty acids in erythrocyte phospholipids during pregnancy and at delivery in mothers and their neonates: comparison with plasma phospholipids. Prostaglandins Leukot Essent Fatty Acids. 2004, 71: 363-374. 10.1016/j.plefa.2004.07.002
Dunstan JA, Mori TA, Barden A, Beilin LJ, Holt PG, Calder PC, Taylor AL, Prescott SL: Effects of n-3 polyunsaturated fatty acid supplementation in pregnancy on maternal and fetal erythrocyte fatty acid composition. Eur J Clin Nutr. 2004, 58: 429-437. 10.1038/sj.ejcn.1601825
Innis SM: Essential fatty acid transfer and fetal development. Placenta. 2005, 26 (Suppl A): S70-75.
van Houwelingen AC, Foreman-van Drongelen MM, Nicolini U, Nicolaides KH, Al MD, Kester AD, Hornstra G: Essential fatty acid status of fetal plasma phospholipids: similar to postnatal values obtained at comparable gestational ages. Early Hum Dev. 1996, 46: 141-152. 10.1016/0378-3782(96)01753-7
Müller A, Keller U, Seliger G, Barthel C, Steinhart H, Eder K: Concentrations of conjugated linoleic acids in neonatal blood in relationship to those in maternal blood. Prostaglandins Leukot Essent Fatty Acids. 2007, 76: 213-219. 10.1016/j.plefa.2007.01.002
Elias SL, Innis SM: Infant plasma trans, n-6, and n-3 fatty acids and conjugated linoleic acids are related to maternal plasma fatty acids, length of gestation, and birth weight and length. Am J Clin Nutr. 2001, 73: 807-814.
Berghaus TM, Demmelmair H, Koletzko B: Fatty acid composition of lipid classes in maternal and cord plasma at birth. Eur J Pediatr. 1998, 157: 763-768. 10.1007/s004310050931
Decsi T, Burus I, Molnar S, Minda H, Veitl V: Inverse association between trans isomeric and long-chain polyunsaturated fatty acids in cord blood lipids of full-term infants. Am J Clin Nutr. 2001, 74: 364-368.
Kuhnt K, Baehr M, Rohrer C, Jahreis G: Trans fatty acid isomers and the trans-9/trans-11 index in fat containing foods. Eur J Lipid Sci Technol. 2011, 113: 1281-1292. 10.1002/ejlt.201100037
Kuhnt K, Kraft J, Moeckel P, Jahreis G: Trans-11-18: 1 is effectively Delta9-desaturated compared with trans-12-18: 1 in humans. Br J Nutr. 2006, 95: 752-761. 10.1079/BJN20051680
Corl BA, Baumgard LH, Dwyer DA, Griinari JM, Phillips BS, Bauman DE: The role of Delta(9)-desaturase in the production of cis-9, trans-11 CLA. J Nutr Biochem. 2001, 12: 622-630. 10.1016/S0955-2863(01)00180-2
Field CJ, Blewett HH, Proctor S, Vine D: Human health benefits of vaccenic acid. Appl Physiol Nutr Metab. 2009, 34: 979-991. 10.1139/H09-079
Turpeinen AM, Mutanen M, Aro A, Salminen I, Basu S, Palmquist DL, Griinari JM: Bioconversion of vaccenic acid to conjugated linoleic acid in humans. Am J Clin Nutr. 2002, 76: 504-510.
Mosley EE, McGuire MK, Williams JE, McGuire MA: Cis-9, trans-11 conjugated linoleic acid is synthesized from vaccenic acid in lactating women. J Nutr. 2006, 136: 2297-2301.
Kanwar RK, Macgibbon AK, Black PN, Kanwar JR, Rowan A, Vale M, Krissansen GW: Bovine milk fat enriched in conjugated linoleic and vaccenic acids attenuates allergic airway disease in mice. Clin Exp Allergy. 2008, 38: 208-218.
Jaudszus A, Krokowski M, Mockel P, Darcan Y, Avagyan A, Matricardi P, Jahreis G, Hamelmann E: Cis-9, trans-11-conjugated linoleic acid inhibits allergic sensitization and airway inflammation via a PPARgamma-related mechanism in mice. J Nutr. 2008, 138: 1336-1342.
Kuhnt K, Wagner A, Kraft J, Basu S, Jahreis G: Dietary supplementation with 11trans- and 12trans-18: 1 and oxidative stress in humans. Am J Clin Nutr. 2006, 84: 981-988.
Rist L, Mueller A, Barthel C, Snijders B, Jansen M, Simoes-Wust AP, Huber M, Kummeling I, von Mandach U, Steinhart H, Thijs C: Influence of organic diet on the amount of conjugated linoleic acids in breast milk of lactating women in the Netherlands. Br J Nutr. 2007, 97: 735-743. 10.1017/S0007114507433074
Thijs C, Mueller A, Rist L, Kummeling I, Snijders BE, Huber M, van Ree R, Simoes-Wust AP, Dagnelie PC, van den Brandt PA: Fatty acids in breast milk and development of atopic eczema and allergic sensitisation in infancy. Allergy. 2011, 66: 58-67. 10.1111/j.1398-9995.2010.02445.x
Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959, 37: 911-917. 10.1139/o59-099
Degen C, Lochner A, Keller S, Kuhnt K, Daenicke S, Jahreis G: Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells. Lipids Health Dis. 2011, 10: 131- 10.1186/1476-511X-10-131
Destaillats F, Golay PA, Joffre F, de Wispelaere M, Hug B, Giuffrida F, Fauconnot L, Dionisi F: Comparison of available analytical methods to measure trans-octadecenoic acid isomeric profile and content by gas-liquid chromatography in milk fat. J Chromatogr A. 2007, 1145: 222-228. 10.1016/j.chroma.2007.01.062
Lakin V, Haggarty P, Abramovich DR, Ashton J, Moffat CF, McNeill G, Danielian PJ, Grubb D: Dietary intake and tissue concentration of fatty acids in omnivore, vegetarian and diabetic pregnancy. Prostaglandins Leukot Essent Fatty Acids. 1998, 59: 209-220. 10.1016/S0952-3278(98)90065-5
Shahin AM, McGuire MK, Anderson N, Williams J, McGuire MA: Effects of margarine and butter consumption on distribution of trans-18:1 fatty acid isomers and conjugated linoleic acid in major serum lipid classes in lactating women. Lipids. 2006, 41: 141-147. 10.1007/s11745-006-5082-x
Hulshof KF, van Erp-Baart MA, Anttolainen M, Becker W, Church SM, Couet C, Hermann-Kunz E, Kesteloot H, Leth T, Martins I: Intake of fatty acids in western Europe with emphasis on trans fatty acids: the TRANSFAIR Study. Eur J Clin Nutr. 1999, 53: 143-157. 10.1038/sj.ejcn.1600692
Craig-Schmidt MC: World-wide consumption of trans fatty acids. Atheroscler Suppl. 2006, 7: 1-4.
Gabriel S: Ermittlung der Aufnahme von trans-Fettsäuren durch Lebensmittel in Deutschland. Diploma thesis. 2009, Friedrich Schiller University Jena, Institute of Nutrition, Department of Nutritional Physiology; in cooperation with the Federal Institute for Risk Assessment (BfR)
Precht D, Molkentin J: Rapid analysis of the isomers of trans-octadecenoic acid in milk fat. Int Dairy J. 1996, 6: 791-809. 10.1016/0958-6946(96)00004-0.
Du ZY, Degrace P, Gresti J, Loreau O, Clouet P: Vaccenic and elaidic acid equally esterify into triacylglycerols, but differently into phospholipids of fed rat liver cells. Lipids. 2011, 46: 647-657. 10.1007/s11745-011-3569-6
Du ZY, Degrace P, Gresti J, Loreau O, Clouet P: Dissimilar properties of vaccenic versus elaidic acid in beta-oxidation activities and gene regulation in rat liver cells. Lipids. 2010, 45: 581-591. 10.1007/s11745-010-3428-x
Mendis S, Cruz-Hernandez C, Ratnayake WM: Fatty acid profile of Canadian dairy products with special attention to the trans-octadecenoic acid and conjugated linoleic acid isomers. J AOAC Int. 2008, 91: 811-819.
Mueller A, Thijs C, Rist L, Simoes-Wust AP, Huber M, Steinhart H: Trans fatty acids in human milk are an indicator of different maternal dietary sources containing trans fatty acids. Lipids. 2010, 45: 245-251. 10.1007/s11745-010-3390-7
Banni S, Carta G, Angioni E, Murru E, Scanu P, Melis MP, Bauman DE, Fischer SM, Ip C: Distribution of conjugated linoleic acid and metabolites in different lipid fractions in the rat liver. J Lipid Res. 2001, 42: 1056-1061.
Innis SM: Metabolic programming of long-term outcomes due to fatty acid nutrition in early life. Matern Child Nutr. 2011, 112-123. 7
Lauritzen L, Carlson SE: Maternal fatty acid status during pregnancy and lactation and relation to newborn and infant status. Matern Child Nutr. 2011, 7 (Suppl 2): 41-58.
Larque E, Perez-Llamas F, Puerta V, Giron MD, Suarez MD, Zamora S, Gil A: Dietary trans fatty acids affect docosahexaenoic acid concentrations in plasma and liver but not brain of pregnant and fetal rats. Pediatr Res. 2000, 47: 278-283. 10.1203/00006450-200002000-00021
Puca AA, Andrew P, Novelli V, Anselmi CV, Somalvico F, Cirillo NA, Chatgilialoglu C, Ferreri C: Fatty acid profile of erythrocyte membranes as possible biomarker of longevity. Rejuvenation Res. 2008, 11: 63-72. 10.1089/rej.2007.0566
Campbell FM, Gordon MJ, Dutta-Roy AK: Preferential uptake of long chain polyunsaturated fatty acids by isolated human placental membranes. Mol Cell Biochem. 1996, 155: 77-83. 10.1007/BF00714336
Cunningham P, McDermott L: Long chain PUFA transport in human term placenta. J Nutr. 2009, 139: 636-639. 10.3945/jn.108.098608
Xu Y, Wang Q, Cook TJ, Knipp GT: Effect of placental fatty acid metabolism and regulation by peroxisome proliferator activated receptor on pregnancy and fetal outcomes. J Pharm Sci. 2007, 96: 2582-2606. 10.1002/jps.20973
Saravanan N, Haseeb A, Ehtesham NZ, Ghafoorunissa : Differential effects of dietary saturated and trans-fatty acids on expression of genes associated with insulin sensitivity in rat adipose tissue. Eur J Endocrinol. 2005, 153: 159-165. 10.1530/eje.1.01946
Lampen A, Leifheit M, Voss J, Nau H: Molecular and cellular effects of cis-9, trans-11-conjugated linoleic acid in enterocytes: effects on proliferation, differentiation, and gene expression. Biochim Biophys Acta. 2005, 1735: 30-40.
Johnsen GM, Weedon-Fekjaer MS, Tobin KA, Staff AC, Duttaroy AK: Long-chain polyunsaturated fatty acids stimulate cellular fatty acid uptake in human placental choriocarcinoma (BeWo) cells. Placenta. 2009, 30: 1037-1044. 10.1016/j.placenta.2009.10.004
Di Nunzio M, Danesi F, Bordoni A: n-3 PUFA as regulators of cardiac gene transcription: a new link between PPAR activation and fatty acid composition. Lipids. 2009, 44: 1073-1079. 10.1007/s11745-009-3362-y
The work has been sponsored by the Ernst-Abbe'-Foundation, the Unna-Foundation and the German Society of Gynaecology and Obstetrics. The authors thank the study participants for taking part in our research study and midwifes of Jena University Hospital obstetrics department for their assistance in the participants recruitment and placenta collection. Further, we thank Udo Markert for his support and Nasim Kroegel for linguistic review of the manuscript.
The authors declare that they have no competing interests.
UE, LS, GJ, and ES designed the study, UE and LS collected the samples. AJ and KK performed fatty acid analysis. UE performed the Prodi evaluation and the statistical analyses. UE, KK and AJ wrote the paper. All authors read and approved the final manuscript.
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Enke, U., Jaudszus, A., Schleussner, E. et al. Fatty acid distribution of cord and maternal blood in human pregnancy: special focus on individual trans fatty acids and conjugated linoleic acids. Lipids Health Dis 10, 247 (2011). https://doi.org/10.1186/1476-511X-10-247
- trans octadecenoic acids
- trans fatty acids
- vaccenic acid
- elaidic acid
- conjugated linoleic acids
- omega-3 LC-PUFA
- t 9/t 11-index
- dairy fat