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Effects of dietary fucoxanthin on cholesterol metabolism in diabetic/obese KK-Ay mice

Lipids in Health and Disease volume 11, Article number: 112 (2012) Cite this article

Abstract

Background

Fucoxanthin is a xanthophyll present in brown seaweeds and has several beneficial effects, including anti-obesity and anti-diabetic effects. However, we and another group previously observed that fucoxanthin increases serum cholesterol levels in rodents. Cholesterol is an important component of cell membranes and biosynthesis of bile acids. Serum cholesterol levels are also closely associated with atherosclerosis. Therefore, we sought to identify the mechanism underlying the increase in serum cholesterol levels by fucoxanthin.

Methods

Diabetic/obese KK-Ay mice were fed a diet containing 0.2% fucoxanthin for 4 weeks. The mice were sacrificed, and total blood samples were collected for the measurement of serum total cholesterol, HDL-cholesterol and non-HDL-cholesterol levels. Cholesterol content in tissues was also analyzed. Real-time PCR and Western blotting were performed to determine hepatic mRNA and protein expression of genes involved in cholesterol metabolism, respectively.

Results

Dietary fucoxanthin significantly increased serum HDL and non-HDL cholesterol levels, and reduced hepatic cholesterol content. In liver, the expression of SREBP1, SREBP2 and their target genes involved in cholesterol biosynthesis significantly increased and tended to increase in the fucoxanthin-fed mice, respectively. In contrast, hepatic levels of LDLR and SR-B1 proteins which is important factors for LDL-cholesterol and HDL-cholesterol uptake in the liver from serum, decreased to 60% and 80% in the fucoxanthin-fed mice, respectively, compared with the control mice. Further, we found that dietary fucoxanthin significantly increased the mRNA expression of proprotein convertase subtilisin/kexin type 9 (PCSK9), which enhances intracellular degradation of LDLR in lysosomes.

Conclusions

Fucoxanthin increased HDL-cholesterol and non-HDL-cholesterol levels in KK-Ay mice by inducing SREBP expression and reduced cholesterol uptake in the liver via down-regulation of LDLR and SR-B1, resulted in increased serum cholesterol in the mice.

Introduction

Fucoxanthin is an orange-colored carotenoid present in edible brown seaweeds, such as Undaria pinnatifida and Hijikia fusiformis. Fucoxanthin has reported to exhibit beneficial health effects, such as anti-cancer [1], anti-inflammatory [2], and radical scavenging [3] activities. We previously reported that fucoxanthin suppresses body weight gain and ameliorates hyperglycemia in diabetic/obese KK-Ay mice [4].

On the other hand, Kadekaru et al. reported that oral fucoxanthin administration for 28 days significantly increased serum total-cholesterol levels in male and female rats [5]. We also observed an increase in serum total cholesterol levels in ICR mice administered a high dose of fucoxanthin (500 mg/kg body weight) for 30 days [6]. In contrast, Woo et al. reported that fucoxanthin (0.2% in diet) decreased plasma cholesterol levels in C57BL/6N mice fed a high-fat diet [7]. Thus, the effects of fucoxanthin on serum cholesterol levels are not clear. In addition, the effects of fucoxanthin on regulation of cholesterol transport and metabolism have not been investigated in detail.

Cholesterol is an essential component of plasma membranes and lipoproteins and is a precursor of internal steroids, such as corticosteroids, sex hormones, bile acids, and vitamin D. Cholesterol levels in the blood and tissues are regulated by a balance between biosynthesis and catabolism, which primarily involve the activities of 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase and cholesterol 7 α-hydroxyrase (CYP7A1), respectively. The expression of genes involved in cholesterol synthesis is strictly regulated by sterol regulatory element binding protein (SREBP) 1 and SREBP2. In addition, low-density lipoprotein (LDL) receptor (LDLR), scavenger receptor class B type 1 (SR-B1), and ATP-binding cassette transporter A1 (ABCA1) play crucial roles in the uptake and efflux of cholesterol in the liver and peripheral tissues to control the cholesterol levels in the body.

In the present study, we investigated the effects of fucoxanthin on the molecular mechanism underlying cholesterol metabolism in diabetic/obese KK-Ay mice. Dietary fucoxanthin (0.2% in the diet) increased HDL-cholesterol and non-HDL-cholesterol levels as well as total cholesterol levels in the serum of KK-Ay mice. Hepatic SREBP2 expression was also increased by fucoxanthin, although hepatic cholesterol content decreased. Fucoxanthin resulted in reduced LDLR expression through up-regulation of hepatic expression of PCSK9 mRNA. In addition, the SR-B1 expression level in the liver was also decreased by fucoxanthin. Our results reveal that fucoxanthin affects on cholesterol metabolism and transport system, which alters the cholesterol balance among the serum, liver, and peripheral tissues.

Materials and methods

Materials

Dried brown seaweed, Undaria pinnatifida, was purchased from a market in Hakodate, Hokkaido in Japan. Anti-SREBP2, anti-LDLR and anti-ABCA1 antibodies were obtained from Abcam (Cambridge, MA, USA). Anti-SREBP1 and anti-SR-B1 were purchased from Santa Cruz Biotechnology (Santa Cruz, California, USA).

Fucoxanthin preparation

Crude lipid containing fucoxanthin was extracted from dried Undaria pinnatifida powder with acetone. Fucoxanthin was purified by silicagel column chromatography with n-hexane/acetone (7:3, v/v) from the crude lipid as our previous report [8]. Purity of fucoxanthin (all-trans- and cis- fucoxanthin) was >95% by HPLC analysis.

Animals and diet

Diabetic/obese KK-Ay mice (4-week old male) were purchased from CLEA Japan, Inc. (Tokyo, Japan). The mice had free access to drinking water and diet at 23 ± 1°C and 50% humidity with a 12 h light/12 h dark cycle. The diet was prepared according to the recommendations of American Institute of Nutrition (AIN-93G). After acclimation for a week by feeding AIN-93G, KK-Ay mice were assigned to two groups and fed the control diet (AIN-93G) and fucoxanthin (0.2%) diet for 4 weeks (Table 1). Blood samples were taken from caudal vena cava of the mice fasted for 12 hour under ether anesthesia. Then, liver, epididymal WAT, and skeletal muscle were removed, weighed and immediately frozen in liquid nitrogen for lipid analysis and Western blotting, or soaked in RNA laterTM (Sigma Chemical Co., St. Louis, MO) for quantitative real time PCR analysis. All procedures for the use and care of animals for this research were approved by the Ethical Committee of Experimental Animal Care at Hokkaido University.

Table 1 Composition of experiment diets based on AIN-93G
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Serum and tissue cholesterol analysis

Serum total-cholesterol and HDL-cholesterol levels were measured by each commercial assay kit (Wako pure chemicals, Osaka, Japan) according to the manufacturer’s instruction. Non-HDL-cholesterol was calculated by subtraction of HDL-cholesterol from total-cholesterol. Cholesterol content in tissue was measured by using a technique as described by Jeon SM et al. [9]. In brief, tissue lipids were extracted by Folch method [10]. An approximately 5 mg lipid was dissolved in 20 μl methanol/Triton X-100 (1:1, v/v) and methanol was removed by nitrogen gas. Distilled-deionized water was then added and mixed until the solution was clear. Cholesterol content was measured using commercial enzymatic assay kit (Wako Pure Chemical Industries, Ltd., Osaka, Japan) according to the manufacturer’s instruction.

Quantitative real-time RT-PCR

Total RNA was isolated from the liver soaked in RNAlater with the RNeasy Mini Kit (Qiagen, Tokyo, Japan), according to the manufacturer’s protocol. Then, cDNA was synthesized from total RNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems Japan Ltd., Tokyo, Japan). Quantitative real-time PCR analysis was carried out using the ABI Prism 7500 (Applied Biosystems Japan Ltd., Tokyo, Japan) with the FastStart Universal Probe Master (Rox) (Roche Diagnostics Japan Ltd., Tokyo, Japan). The mRNA expression levels were measured using the Taqman® Gene Expression Assays (Applied Biosystems Japan Ltd, Tokyo, Japan); Hmgcr: Mm01282499_m1, Hmgcs: Mm01304569_m1, Fdps: Mm00836315_g1, Lss: Mm00461312_m1, Cyp51:Mm00490968_m1, Cyp7a1: Mm00484152_m1, PCSK9: Mm01263610_m1 and β-Actin: Mm00607939_s1.

Western blotting

Liver was suspended in 10 volumes of a buffer containing 50 mM Hepes, 150 mM NaCl, 1 mM EDTA, 2 mM EGTA, 50 mM sodium fluoride, 1% Triton X-100 and 1 mM phenylmethylsulfonyl fluoride supplemented protease inhibitor cocktail (Sigma Chemical Co., St. Louis, MO, USA). After centrifugation at 8,550 g for 60 min at 4°C, the supernatant was collected for assay. Protein concentration in the supernatant was determined by a colorimetric method using a DC protein assay kit (Bio-Rad Laboratories, Hercules, CA, USA). The lysates were mixed with the same volume of loading buffer (Sigma Chemical Co., St. Louis, MO, USA), and boiled for 5 min. Protein samples were loaded at 30 μg protein per lane, and separated by SDS-polyacrylamide (10%) gel electrophoresis. Thereafter, proteins were transferred onto a polyvinylidene difluoride membrane. The membrane was blocked with 0.5% non-fat dry milk in TBS buffer for 1 hour at room temperature. For detection of protein, the membranes were incubated with primary anti-bodies for 1 hour at room temperature. After a washing procedure, the membrane was incubated with horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, California, USA) for 1 hour at room temperature. The immunoreactive bands were visualized with ECL Plus Western Blotting Detection System (GE Healthcare Japan Corporation, Japan). Quantitative densitometric analyses were performed on digitized images of immunoblots using cooled CCD camera system (ATTO corporation, Tokyo, Japan). The β-Actin was used for loading standardization.

Statistical analysis

Data are presented as the mean ± SEM. The statistical significance between control and fucoxanthin groups was analyzed by Student’s t-tests for each experiment.

Results

Cholesterol level in the serum, liver, white adipose tissue and muscle

Fucoxanthin (0.2% in AIN-93G diet) suppressed body weight gain of diabetic/obese KK-Ay mice as previous papers [4]. Food intake of fucoxanthin-fed mice tended to be lower, but not significantly, than that of control mice. Dietary fucoxanthin significantly increased serum total cholesterol levels in the KK-Ay mice (Table 2). HDL-cholesterol and non-HDL-cholesterol levels also increased in the fucoxanthin-fed mice. In contrast, hepatic cholesterol content was significantly lower in the fucoxanthin-fed mice than in the control mice (Table 3), although liver weight increased by fucoxanthin as our previous study [6]. These effects of fucoxanthin were associated with the amount of cholesterol per unit protein in the liver of the fucoxanthin-fed (26.36 ± 0.91) and control mice (50.59 ± 5.46), respectively. The mechanism by which fucoxanthin impacts on the liver is unclear.

Table 2 Total, HDL, and non-HDL cholesterol concentrations in the serum
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Table 3 Tissue weights and cholesterol contents in the liver, epididymal WAT, and muscle
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The total amount of cholesterol that had accumulated in epididymal WAT was also lower in the fucoxanthin-fed mice than in the control mice because epididymal WAT weight in the fucoxanthin-fed mice was lower than that in the control mice (Table 3). On the other hand, cholesterol level in skeletal muscle was not altered by fucoxanthin. These results show that fucoxanthin reduces the total cholesterol accumulation in the liver and WAT.

Gene and protein expressions involved in cholesterol metabolism in the liver

The expression levels of genes related to cholesterol biosynthesis and catabolism in the liver were analyzed by real-time quantitative PCR (Figure 1A). The mRNA expression level of HMG-CoA reductase (HMGCR), a rate-limiting enzyme in cholesterol synthesis, tended to increase in the fucoxanthin group. In addition, except that of lanosterol synthase (LSS), the mRNA expression level of key enzymes involved in cholesterol biosynthesis, including HMG-CoA synthase (HMGCS), farnesyl diphosphate synthase (FDPS), and lanosterol 14α-demethylase (CYP51), tended to be increased by fucoxanthin. In contrast, the mRNA expression level of CYP7A1, an enzyme associated with synthesis of bile acids from cholesterol, did not increase by fucoxanthin-diet (Figure 1B). Overall, the mRNA expression of enzymes involved in cholesterol synthesis tended to be higher in the fucoxanthin-fed mice, although not significantly.

Figure 1
figure 1

Hepatic gene expression involved in cholesterol metabolism. KK-Ay mice were fed with the AIN-93G diet with or without 0.2% fucoxanthin for 4 weeks. Total RNA was extracted from the liver and processed for quantitative real-time PCR analysis as described under “Materials and Methods”. The expression levels of each mRNA are normalized to β-actin levels. Data are presented relative to the control group. Each value represents the mean ± standard error (n = 6). HMGCR: HMG-CoA reductase, HMGCS: HMG-CoA synthase, FDPS: farnesyl diphosphate synthase, LSS: lanosterol synthase, CYP51: lanosterol 14α-demethylase, CYP7A1: cholesterol 7 α-hydroxyrase.

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We further examined the expression levels of SREBP1 and SREBP2, which are key transcriptional factors for the regulation of cholesterol metabolism. As shown in Figure 2, dietary fucoxanthin resulted in a 2.7-fold increase in SREBP2 expression compared with control mice. SREBP1 expression was also significantly increased in the fucoxanthin-fed mice. In particular, SREBP2 is known to be sensitively activated and to enhance gene transcription for cholesterol synthesis when cellular cholesterol levels decline [11, 12]. Our results show that SREBP2 and SREBP1 expression was enhanced in the liver by fucoxanthin-diet, although hepatic cholesterol content in the mice fed fucoxanthin was lower than that in the control mice.

Figure 2
figure 2

SREBP1 and SREBP2 expressions in the liver. KK-Ay mice were fed with the AIN-93G diet with or without 0.2% fucoxanthin for 4 weeks. Liver lysates were prepared for Western blotting, and primary antibody binding was detected by appropriate secondary antibodies for chemiluminescence as described under “Materials and Methods”. SREBP1 and SREBP2 levels are presented relative to control group after normalization for β-Actin levels. Each value represents the mean ± standard error (n = 6). Asterisks show significant differences from the control group (** P < 0.01).

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LDLR, SR-B1, and ABCA1 expressions involved in cholesterol uptake and transport in the liver

To understand how serum cholesterol levels increased and hepatic cholesterol content decreased by fucoxanthin, we next examined cholesterol uptake-related proteins, LDLR and SR-B1, which are receptors for LDL and cholesteryl esters from HDL in the liver. LDLR expression levels in the liver were markedly decreased by fucoxanthin (Figure 3). SR-B1 expression levels were also significantly lower in the KK-Ay mice fed fucoxanthin than in the control mice (Figure 3). The reduced LDLR and SR-B1 expression levels in the liver indicated that fucoxanthin suppresses the uptake of LDL particles and HDL-cholesterol from the blood into the liver. On the other hand, the expression levels of ABCA1, which is a key factor for cholesterol efflux from the liver [13], were not significantly different between the control and fucoxanthin group.

Figure 3
figure 3

Effect of fucoxanthin on expression levels of cholesterol transport-related proteins in the liver. KK-Ay mice were fed with the AIN-93G diet with or without 0.2% fucoxanthin for 4 weeks. Liver lysates were prepared for Western blotting, and primary antibody binding was detected by appropriate secondary antibodies for chemiluminescence as described under “Materials and Methods”. Expression levels are presented relative to control group after normalization for β-Actin levels. Each value represents the mean ± standard error (n = 6). Asterisks show significant differences from the control group (* P < 0.05, ** P < 0.01).

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Hepatic expression of PCSK9 mRNA involved in LDLR degradation

PCSK9, a member of the subtilisin family of serine proteases, has been shown to induce intracellular degradation of LDLR [14, 15]. Fucoxanthin induced a 2-fold increase in hepatic expression of PCSK9 mRNA (Figure 4). This result suggests that reduced hepatic LDLR expression was due to PCSK9-associated LDLR degradation induced by fucoxanthin.

Figure 4
figure 4

Effect of fucoxanthin on expression levels of PCSK9 mRNA in the liver. KK-Ay mice were fed with the AIN-93G diet with or without 0.2% fucoxanthin for 4 weeks. Total RNA was extracted from the liver and processed for quantitative real time PCR analysis as described under “Materials and Methods”. PCSK9 mRNA expression levels are normalized to β-actin levels. Data are presented relative to control group. Each value represents the mean ± standard error (n = 6). Asterisks show significant differences from the control group (** P < 0.01).

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Discussion

Fucoxanthin from edible brown seaweeds have reported to show anti-obesity effect on diabetic/obese KK-Ay mice and high-fat-fed C57BL/6N mice [7, 16]. A fucoxanthin and conjugated linolenic acid-containing supplement has also been reported to induce weight loss and reduce the body and liver fat levels in obese non-diabetic women [17]. On the other hand, we and another group observed that fucoxanthin increases serum total cholesterol levels in rodents [5, 6]. Cholesterol is an important component of cell membranes and is required for the biosynthesis of bile acids and vitamin D. However, an excessive serum cholesterol level, particularly LDL cholesterol, is known to be a risk factor for atherosclerosis. Therefore, we investigated the mechanisms underlying the increase in serum cholesterol levels induced by fucoxanthin.

In this study, we observed increased HDL-cholesterol and non-HDL-cholesterol levels as well as total cholesterol levels in the serum of diabetic/obese KK-Ay mice fed fucoxanthin. Dietary fucoxanthin also significantly increased the expression of SREBP2 and SREBP1, which are key transcriptional factors involved in up-regulation of cholesterol synthesis. Further, the mRNA expression levels of HMGCR, HMGCS, FDPS, and CYP51, which are involved in cholesterol synthesis, tended to increase in the fucoxanthin-fed mice compared with the control mice, although these differences were not statistically significant. These results show that fucoxanthin slightly enhances cholesterol synthetic pathway in the liver. In contrast, hepatic cholesterol content decreased in the fucoxanthin-diet group.

To determine how fucoxanthin regulated cholesterol metabolism we considered the balance between cholesterol biosynthesis, efflux, and its incorporation in tissues. SR-B1 is known to play an important role in selectively incorporating cholesteryl esters from circulating HDL into cells. Mice with attenuated hepatic SR-B1 expression had reduced selective HDL-cholesterol clearance and increased HDL-cholesterol levels in their blood [18, 19]. Conversely, plasma HDL-cholesterol levels were dramatically decreased in mice that overexpressed hepatic SR-B1 [20]. Non-HDL-cholesterol levels, mostly LDL-cholesterol levels are determined, in part, by the rate at which LDL particles are taken up and removed from the circulation by LDLR [21]. The SR-B1 and LDLR proteins expressed in the liver at high levels and their hepatic expressions have a significant impact on circulating cholesterol levels [22]. In the present study, fucoxanthin resulted in reduced SR-B1 and LDLR expression in the liver of the KK-Ay mice, whereas no differences were observed in the cholesterol efflux factor ABCA1 [23]. These results show that the increased serum cholesterol levels resulted from the reduced hepatic clearance of serum cholesterol via down-regulation of SR-B1 and LDLR by fucoxanthin. In particular, the decrease in hepatic cholesterol content suggests that the reduction of incorporation of serum cholesterol may predominate over the endogenous cholesterol biosynthesis in KK-Ay mice.

Adipose tissue is also known to store large amounts of cholesterol and contributes to regulation of circulating cholesterol levels [24, 25]. Dietary fucoxanthin suppressed the enlargement of visceral WAT during the development of obesity, which resulted in attenuation of cholesterol estimations in WAT. On the other hand, dietary fucoxanthin also increased the serum cholesterol levels in non-obese ICR mice without suppressing their body weight gain [6]. Taken together, these results suggest that the increase in serum cholesterol levels observed in the KK-Ay mice were partly due to the suppression of fat accumulation by dietary fucoxanthin.

Thus, we have shown for the first time that dietary fucoxanthin leads to increased HDL and non-HDL-cholesterol levels in KK-Ay mice through down-regulation of hepatic LDLR and SR-B1 expression, while fucoxanthin increases the expression of SREBP2, which up-regulates LDLR. As one possible mechanism, we propose that LDLR post-transcriptional regulation is involved.

Recent studies have demonstrated LDLR post-transcriptional regulation by proprotein convertase subtilisin/kexin type 9 (PCSK9) [26, 27] and LXR/inducible degrader of LDLR (IDOL) signaling pathways [28]. PCSK9 is primarily expressed in the liver, small intestine, and kidneys [29]. PCSK9 binds to the EGF-A extracellular domain of LDLR and subsequently triggers its intracellular degradation in lysosomes [30]. In addition, PCSK9 overexpression in mice reduced LDLR levels and increased non-HDL-cholesterol levels [31]. Interestingly, PCSK9 has been identified as a SREBP target gene [32, 33]. It is noteworthy that PCSK9 mRNA expression was up-regulated by fucoxanthin. These results suggest that fucoxanthin promotes LDLR degradation through PCSK9 mRNA up-regulation in the SREBP2 signaling pathway. Additional studies are needed to elucidate the mechanism associated with PCSK9 mRNA up-regulated by fucoxanthin.

On the other hand, it has been reported that hepatic SR-B1 expression is regulated by a variety of dietary components, hormonal, metabolic, and pharmacological manipulations [22, 34]. Nuclear receptors components such as liver X receptor (LXR) and peroxisome proliferator-activated receptor-α (PPARα) are also known to regulate hepatic SR-B1 expression [35, 36]. In this study, we could not identify a key factor to suppress SR-B1 by fucoxanthin. Further investigation is required to clarify the effect of fucoxanthin on SR-B1 expression.

In this study, we showed for the first time that dietary fucoxanthin resulted in increased serum HDL and non-HDL-cholesterol levels as well as total cholesterol levels via the activation of SREBP signaling and by suppressing serum cholesterol uptake in the liver via decreasing LDLR and SR-B1 expression. Further, our results suggest that LDLR degradation is promoted by fucoxanthin through up-regulation of PCSK9 and leads to increased non-HDL-cholesterol levels. These findings provide important insights on the effects of fucoxanthin on cholesterol and lipoprotein metabolism. However, it is unclear whether the responses to cholesterol metabolism are specific for rodents or common to human. Dysfunction of cholesterol metabolism is strongly associated with arteriosclerosis. Therefore, it will be necessary to further investigate the influence of high serum cholesterol levels induced by fucoxanthin on human health.

Abbreviations

HDL:

High-density lipoprotein

SREBP:

Sterol regulatory element binding protein

LDLR:

Low-density lipoprotein receptor

SR-B1:

Scavenger receptor class B type 1

LDL:

Low-density lipoprotein

PCSK9:

Proprotein convertase subtilisin/kexin type 9

HMG-CoA:

3-hydroxy-3-methyl-glutaryl-CoA

CYP7A1:

Cholesterol 7 α-hydroxyrase

ABCA1:

ATP-binding cassette transporter A1

AIN:

American Institute of Nutrition

WAT:

White adipose tissue

HMGCR:

HMG-CoA reductase

LSS:

Lanosterol synthase

HMGCS:

HMG-CoA synthase

FDPS:

Farnesyl diphosphate synthase

CYP51:

Lanosterol 14α-demethylase

IDOL:

LXR/inducible degrader of LDLR.

References

  1. Hosokawa M, Kudo M, Maeda H, Kohno H, Tanaka T, Miyashita K: Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARgamma ligand, troglitazone, on colon cancer cells. Biochim Biophys Acta. 2004, 1675: 113-119. 10.1016/j.bbagen.2004.08.012

    Article  CAS  PubMed  Google Scholar 

  2. Shiratori K, Ohgami K, Ilieva I, Jin XH, Koyama Y, Miyashita K, Yoshida K, Kase S, Ohno S: Effects of fucoxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo. Exp Eye Res. 2005, 81: 422-428. 10.1016/j.exer.2005.03.002

    Article  CAS  PubMed  Google Scholar 

  3. Sachindra NM, Sato E, Maeda H, Hosokawa M, Niwano Y, Kohno M, Miyashita K: Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites. J Agric Food Chem. 2007, 55: 8516-8522. 10.1021/jf071848a

    Article  CAS  PubMed  Google Scholar 

  4. Hosokawa M, Miyashita T, Nishikawa S, Emi S, Tsukui T, Beppu F, Okada T, Miyashita K: Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-Ay mice. Arch Biochem Biophys. 2010, 504: 17-25. 10.1016/j.abb.2010.05.031

    Article  CAS  PubMed  Google Scholar 

  5. Kadekaru T, Toyama H, Yasumoto T: Safety evaluation of fucoxanthin purified from undariapinnatifida. Nippon Shokuhin Kagaku Kogaku Kaishi. 2008, 55: 304-308. 10.3136/nskkk.55.304. 10.3136/nskkk.55.304

    Article  CAS  Google Scholar 

  6. Beppu F, Niwano Y, Tsukui T, Hosokawa M, Miyashita K: Single and repeated oral dose toxicity study of fucoxanthin (FX), a marine carotenoid, in mice. J Toxicol Sci. 2009, 34: 501-510. 10.2131/jts.34.501

    Article  CAS  PubMed  Google Scholar 

  7. Woo MN, Jeon SM, Kim HJ, Lee MK, Shin SK, Shin YC, Park YB, Choi MS: Fucoxanthin supplementation improves plasma and hepatic lipid metabolism and blood glucose concentration in high-fat fed C57BL/6 N mice. Chem Biol Interact. 2010, 186: 316-322. 10.1016/j.cbi.2010.05.006

    Article  CAS  PubMed  Google Scholar 

  8. Tsukui T, Konno K, Hosokawa M, Maeda H, Sashima T, Miyashita K: Fucoxanthin and fucoxanthinol enhance the amount of docosahexaenoic acid in the liver of KKAy obese/diabetic mice. J Agric Food Chem. 2007, 55: 5025-5029. 10.1021/jf070110q

    Article  CAS  PubMed  Google Scholar 

  9. Jeon SM, Kim HJ, Woo MN, Lee MK, Shin YC, Park YB, Choi MS: Fucoxanthin-rich seaweed extract suppresses body weight gain and improves lipid metabolism in high-fat-fed C57BL/6 J mice. Biotechnol J. 2010, 5: 961-969. 10.1002/biot.201000215

    Article  CAS  PubMed  Google Scholar 

  10. Folch J, Lees M, Sloane Stanley GH: A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957, 226: 497-509.

    CAS  PubMed  Google Scholar 

  11. Hua X, Yokoyama C, Wu J, Briggs MR, Brown MS, Goldstein JL, Wang X: SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc Natl Acad Sci USA. 1993, 90: 11603-11607. 10.1073/pnas.90.24.11603

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Brown MS, Goldstein JL: The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell. 1997, 89: 331-340. 10.1016/S0092-8674(00)80213-5

    Article  CAS  PubMed  Google Scholar 

  13. Timmins JM, Lee JY, Mulya A: Tissue-specific hepatic deletion of ABCA1 indicates that the liver is the primary site for HDL formation in vivo [abstract]. Arterioscler Thromb Vasc Biol. 2004, 24: 52-

    Google Scholar 

  14. Brown MS, Goldstein JL: Lowering LDL – not only how low, but how long?. Science. 2006, 311: 1721-1723. 10.1126/science.1125884

    Article  CAS  PubMed  Google Scholar 

  15. Attie AD, Seidah NG: Dual regulation of the LDL receptor–some clarity and new questions. Cell Metab. 2005, 1: 290-292. 10.1016/j.cmet.2005.04.006

    Article  CAS  PubMed  Google Scholar 

  16. Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K: Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem Biophys Res Commun. 2005, 332: 392-397. 10.1016/j.bbrc.2005.05.002

    Article  CAS  PubMed  Google Scholar 

  17. Abidov M, Ramazanov Z, Seifulla R, Grachev S: The effects of Xanthigen in the weight management of obese premenopausal women with non-alcoholic fatty liver disease and normal liver fat. Diabetes Obes Metab. 2010, 12: 72-81. 10.1111/j.1463-1326.2009.01132.x

    Article  CAS  PubMed  Google Scholar 

  18. Rigotti A, Trigatti BL, Penman M, Rayburn H, Herz J, Krieger M: A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism. Proc Natl Acad Sci USA. 1997, 94: 12610-12615. 10.1073/pnas.94.23.12610

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Varban ML, Rinninger F, Wang N, Fairchild-Huntress V, Dunmore JH, Fang Q, Gosselin ML, Dixon KL, Deeds JD, Acton SL, Tall AR, Huszar D: Targeted mutation reveals a central role for SR-BI in hepatic selective uptake of high density lipoprotein cholesterol. Proc Natl Acad Sci USA. 1998, 95: 4619-4624. 10.1073/pnas.95.8.4619

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  20. Wang N, Arai T, Ji Y, Rinninger F, Tall AR: Liver-specific overexpression of scavenger receptor BI decreases levels of very low density lipoprotein ApoB, low density lipoprotein ApoB, and high density lipoprotein in transgenic mice. J Biol Chem. 1998, 273: 32920-32926. 10.1074/jbc.273.49.32920

    Article  CAS  PubMed  Google Scholar 

  21. Brown MS, Goldstein JL: A receptor-mediated pathway for cholesterol homeostasis. Science. 1986, 232: 34-47. 10.1126/science.3513311

    Article  CAS  PubMed  Google Scholar 

  22. Rigotti A, Miettinen HE, Krieger M: The role of the high-density lipoprotein receptor SR-BI in the lipid metabolism of endocrine and other tissues. Endocr Rev. 2003, 24: 357-387. 10.1210/er.2001-0037

    Article  CAS  PubMed  Google Scholar 

  23. Soumian S, Albrecht C, Davies AH, Gibbs RG: ABCA1 and atherosclerosis. Vasc Med. 2005, 10: 109-119. 10.1191/1358863x05vm593ra

    Article  CAS  PubMed  Google Scholar 

  24. Quintão E, Grundy SM, Ahrens EH: Effects of dietary cholesterol on the regulation of total body cholesterol in man. J Lipid Res. 1971, 12: 233-247.

    PubMed  Google Scholar 

  25. Farkas J, Angel A, Avigan MI: Studies on the compartmentation of lipid in adipose cells. II. Cholesterol accumulation and distribution in adipose tissue components. J Lipid Res. 1973, 14: 344-356.

    CAS  PubMed  Google Scholar 

  26. Benjannet S, Rhainds D, Essalmani R, Mayne J, Wickham L, Jin W, Asselin MC, Hamelin J, Varret M, Allard D, Trillard M, Abifadel M, Tebon A, Attie AD, Rader DJ, Boileau C, Brissette L, Chrétien M, Prat A, Seidah NG: NARC-1/PCSK9 and its natural mutants: zymogen cleavage and effects on the low density lipoprotein (LDL) receptor and LDL cholesterol. J Biol Chem. 2004, 279: 48865-48875. 10.1074/jbc.M409699200

    Article  CAS  PubMed  Google Scholar 

  27. Park SW, Moon YA, Horton JD: Post-transcriptional regulation of low density lipoprotein receptor protein by proprotein convertase subtilisin/kexin type 9a in mouse liver. J Biol Chem. 2004, 279: 50630-50638. 10.1074/jbc.M410077200

    Article  CAS  PubMed  Google Scholar 

  28. Zelcer N, Hong C, Boyadjian R, Tontonoz P: LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor. Science. 2009, 325: 100-104. 10.1126/science.1168974

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Seidah NG, Benjannet S, Wickham L, Marcinkiewicz J, Jasmin SB, Stifani S, Basak A, Prat A, Chretien M: The secretory proprotein convertase neural apoptosis-regulated convertase 1 (NARC-1): liver regeneration and neuronal differentiation. Proc Natl Acad Sci USA. 2003, 100: 928-933. 10.1073/pnas.0335507100

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  30. Zhang DW, Lagace TA, Garuti R, Zhao Z, McDonald M, Horton JD, Cohen JC, Hobbs HH: Binding of proprotein convertase subtilisin/kexin type 9 to epidermal growth factor-like repeat A of low density lipoprotein receptor decreases receptor recycling and increases degradation. J Biol Chem. 2007, 282: 18602-18612. 10.1074/jbc.M702027200

    Article  CAS  PubMed  Google Scholar 

  31. Park SW, Moon YA, Horton JD: Post-transcriptional regulation of low density lipoprotein receptor protein by proprotein convertase subtilisin/kexin type 9a in mouse liver. J Biol Chem. 2004, 279: 50630-50638. 10.1074/jbc.M410077200

    Article  CAS  PubMed  Google Scholar 

  32. Horton JD, Shah NA, Warrington JA, Anderson NN, Park SW, Brown MS, Goldstein JL: Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes. Proc Natl Acad Sci USA. 2003, 100: 12027-12032. 10.1073/pnas.1534923100

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  33. Maxwell KN, Soccio RE, Duncan EM, Sehayek E, Breslow JL: Novel putative SREBP and LXR target genes identified by microarray analysis in liver of cholesterol-fed mice. J Lipid Res. 2003, 44: 2109-2119. 10.1194/jlr.M300203-JLR200

    Article  CAS  PubMed  Google Scholar 

  34. Trigatti B, Rigotti A, Krieger M: The role of the high-density lipoprotein receptor SR-BI in cholesterol metabolism. Curr Opin Lipidol. 2000, 11: 123-131. 10.1097/00041433-200004000-00004

    Article  CAS  PubMed  Google Scholar 

  35. Malerød L, Juvet LK, Hanssen-Bauer A, Eskild W, Berg T: Oxysterol-activated LXRalpha/RXR induces hSR-BI-promoter activity in hepatoma cells and preadipocytes. Biochem Biophys Res Commun. 2002, 299: 916-923. 10.1016/S0006-291X(02)02760-2

    Article  PubMed  Google Scholar 

  36. Mardones P, Pilon A, Bouly M, Duran D, Nishimoto T, Arai H, Kozarsky KF, Altayo M, Miquel JF, Luc G, Clavey V, Staels B, Rigotti A: Fibrates down-regulate hepatic scavenger receptor class B type I protein expression in mice. J Biol Chem. 2003, 278: 7884-7890. 10.1074/jbc.M211627200

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by Japan Society for the Promotion of Science (JSPS) Grant-in Aid for JSPS Fellows (2867).

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Authors and Affiliations

  1. Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato Hakodate, Hokkaido, 041-8611, Japan

    Fumiaki Beppu, Masashi Hosokawa & Kazuo Miyashita

  2. Laboratory for Redox Regulation, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-cho, Aoba-ku, Sendai, 980-8575, Japan

    Yoshimi Niwano

Authors
  1. Fumiaki Beppu
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  2. Masashi Hosokawa
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  3. Yoshimi Niwano
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  4. Kazuo Miyashita
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Correspondence to Masashi Hosokawa.

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The authors declare that they have no competing interests.

Authors’ contributions

FB conceived the study, its design and coordination, performed all experiments. MH participated in the design and coordination of the study and discussion of results. FB and MH discussed results and made the manuscript. YN and KM participated in the coordination of the study. All authors read and approved the final manuscript.

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Beppu, F., Hosokawa, M., Niwano, Y. et al. Effects of dietary fucoxanthin on cholesterol metabolism in diabetic/obese KK-Ay mice. Lipids Health Dis 11, 112 (2012). https://doi.org/10.1186/1476-511X-11-112

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  • DOI: https://doi.org/10.1186/1476-511X-11-112

Keywords

Lipids in Health and Disease

ISSN: 1476-511X