Sildenafil ameliorates biomarkers of genotoxicity in an experimental model of spontaneous atherosclerosis
© Rodrigues et al.; licensee BioMed Central Ltd. 2013
Received: 20 July 2013
Accepted: 23 August 2013
Published: 28 August 2013
It is well known that enhanced production of reactive oxygen species (ROS) leads to oxidative stress observed in atherosclerosis and that ROS can also cause damage in cellular macromolecules, including DNA. Considering previous report that sildenafil, an inhibitor of phosphodiesterase 5 (PDE5), has antioxidant effects, in the present study we evaluated the effect of this drug on genotoxicity of blood mononuclear cells (MNC) and liver cells from atherosclerotic apolipoprotein E knockout mice (apoE-/-).
ROS production in MNC was evaluated by flow cytometry with the fluorescent dye dihydroethidium (DHE), a method that has been used to quantify the production of superoxide anion, and DNA damage was evaluated in both MNC and liver cells using the alkaline comet assay. Sildenafil-administered apoE-/- mice were compared with strain-matched mice administered with vehicle and with C57BL/6 wild-type (WT) mice.
MNC from apoE-/- vehicle exhibited a 2-fold increase in production of superoxide anion in comparison with WT. In contrast, sildenafil-administered apoE-/- mice showed superoxide anion levels similar to those observed in WT mice. Similarly, MNC and liver cells from apoE-/- vehicle mice showed a 4-fold and 2-fold augmented DNA fragmentation compared with WT, respectively, and sildenafil-administered apoE-/- mice exhibited minimal DNA damage in those cells similar to WT mice.
ApoE-/- mice chronically administered with sildenafil exhibited reduced levels of superoxide anion in MNC and less DNA fragmentation in MNC and liver cells, which are biomarkers of genotoxicity. Therefore, sildenafil may offer a new perspective to the use of PDE5 inhibitors to protect against DNA damage, in cells involved in the inflammatory and dyslipidemic processes that accompany atherosclerosis.
Hypercholesterolemia and atherosclerosis results from metabolic disorders, enhanced oxidative stress and inflammation [1–3]. Experimentally, the apolipoprotein E knockout mouse (apoE-/-) has been widely used in studies aiming to better understand this disease and to propose new treatment approaches. In this model, the atherosclerotic process increases continuously and the progression of lesions is accelerated under Western-type diet [4–6].
Experimental and clinical evidences support the hypothesis that lipid-oxidation products, obtained endogenously or ingested with food, increases incidence of atherosclerosis [7–10] and even tumor frequency [8, 9]. These effects are justified by genotoxicity in various locals, including blood cells and hepatocytes . Furthermore, it has been suggested that the excessive generation of reactive oxygen species (ROS), leading to the oxidative stress play an important role in the induction of DNA damage [6, 11].
Oxidative stress is the result of an imbalance between the production of oxidant species and antioxidant defences, with predominance of ROS [6, 12]. High levels of ROS are important mediators of damage in cell components such as carbohydrates, lipids, proteins and nucleic acids . Oxidative damage to DNA can occur in different ways, causing oxidation of specific bases or strand breaks, leading to genomic instability and permanent changes in the genetic material (genotoxicity) . Thus, in conditions of increased oxidative stress, as observed in atherosclerosis, antioxidant alternative strategies could be convenient to reduce oxidative stress and to prevent the genetic material damage. Experimentally, the comet assay evaluates DNA damage, which is a biomarker of genotoxicity, in individual cells through the measurement of DNA migration in gel electrophoresis . Although it has been usually performed in blood cells, which are easily obtained to demonstrate systemic genotoxic damage, other tissues can also be used, as the effects of genotoxicity are tissue-specific [16, 17]. Liver is also considered a target organ for genotoxicity research, specifically in atherosclerosis, as this is the main organ of lipid metabolism [16, 17].
Recently our laboratory showed that sildenafil, a phosphodiesterase 5 (PDE5) inhibitor which has been widely used for erectile dysfunction and pulmonary hypertension treatment [18, 19], restores endothelial function in apoE-/- mice . Considering experimental evidence that this drug can prevent oxidative stress induction and lipid peroxidation [19, 20], sildenafil could be a promising pharmacological alternative to prevent ROS-induced DNA damage in atherosclerosis. Therefore, the aim of the present study was to evaluate the effect of sildenafil on genotoxicity induced by oxidative stress of mononuclear cells (MNC) and liver cells of atherosclerotic apoE-/- mice.
Plasma Lipid profile
52 ± 6.1
299 ± 47**
248 ± 52*
Total cholesterol (mg/dL)
100 ± 7.8
1229 ± 185**
1310 ± 222**
34 ± 7.2
188 ± 49**
217 ± 48.6**
49 ± 2.8
24 ± 8**
10 ± 0.9**
VLDL + IDL (mg/dL)
18 ± 5.5
1016 ± 195**
1083 ± 240**
Reactive oxygen species (ROS)
Analysis of DNA damage by comet assay
Another parameter analyzed was the comet tail moment, an index of both the migration of the genetic material and the relative amount of DNA in the tail . This analysis revealed a decrease of DNA fragmentation in animals administered with sildenafil in both MNC (Figure 2C: WT: 0.9 ± 0.2 a.u. vs. apoE-/- vehicle: 3.9 ± 0.7 a.u., p < 0.01 vs. apoE-/- sildenafil: 0.7 ± 0.2 a.u., p < 0.01) and liver cells (Figure 3C: WT: 2.1 ± 0.4 a.u. vs. apoE-/- vehicle: 5.5 ± 1.0 a.u., p < 0.05 vs. apoE-/- sildenafil: 1.9 ± 0.3 a.u., p < 0.01). In addition, we also quantified the comets with moderate-to-high damage based on the cutoff of more than 25% of DNA on the tail. This parameter is summarized in the Figure 2D, which shows that the number of comets with more than 25% of DNA on the tail in MNC were significantly higher in apoE-/- vehicle than in WT control mice (5.5 ± 1.2 vs. 0.8 ± 0.5, p < 0.01) and normalized in apoE-/- sildenafil mice (0.7 ± 0.3, p < 0.01). Similar results were observed in liver cells, as shown in Figure 3D (WT: 2.2 ± 0.7 vs. apoE-/- vehicle: 6.8 ± 1.1, p < 0.01 vs. apoE-/- sildenafil: 2.0 ± 0.6, p < 0.01). Taken together, these results demonstrate that chronic administration of sildenafil is able to reduce DNA fragmentation in MNC and liver cells of apoE-/- mice.
Cumulative evidence suggests that DNA instability plays an important role in chronic degenerative diseases as atherosclerosis [29, 30]. In this study, we reported for the first time that chronic inhibition of PDE5 with sildenafil can decrease genotoxicity in MNC and liver cells in vivo, in atherosclerotic apoE-/- mouse model.
Interestingly, sildenafil did not change the plasma lipid profile in apoE-/- mice, which is consistent with results previously reported by our laboratory  and by others . Although Ronsein et al.  have proposed that hypercholesterolemia directly contributes to the DNA damage, which was also demonstrated in mice [11, 17], rats  and rabbits , in the present study we show evidence that the amelioration in oxidative stress and DNA damage by sildenafil might be independent of hypercholesterolemia.
Elevated levels of ROS, which is a characteristic of a state of oxidative stress, has been reported in clinical and pre-clinical studies in many cardiovascular diseases, including diabetes, hypertension and atherosclerosis [3, 11, 24, 34, 35]. Although under physiological conditions ROS are continuously produced in most cells, the imbalance between ROS production and its degradation, can lead to genomic instability and, consequently, permanent changes in the genetic material, contributing to unfavourable processes, e.g. apoptosis, observed in different target tissues of the cardiovascular diseases [17, 36].
Oxidative DNA damage can result from a variety of factors including radiation, toxins, chemicals and ROS, by products of normal metabolic processes . It is well-known that DNA damage can occur in cells exposed to oxidative stress and the oxidative DNA damage has been estimated as 104 hits per cell per day in humans; in this way, oxidative stress could be the main contributor to DNA damage in cardiovascular diseases [30, 38, 39].
Recently, we reported that sildenafil appears to involve an enhancement of the nitric oxide (NO) pathway along with a reduction in oxidative stress . Moreover, it has been shown that increased intracellular levels of cGMP can inhibit NADPH oxidase expression/activity [20, 40, 41], increase enzyme activities of superoxide dismutase, catalase and glutathione peroxidase , thereby reducing superoxide anion bioavailability. The antioxidant properties of sildenafil  could constitute a key mechanism for the decrease of oxidative stress and DNA damage that we observed in MNC and liver cells. Another potential advantage of a therapeutic approach based on inhibition of PDE5 is that, unlike strategies that attempt to increase NO levels (e.g., substrates, co-factors, antioxidants) [42, 43], this one avoids risks associated with NO, which in excess is a cytotoxic free radical which possess the characteristic of a “double-edged sword” . Accordingly, it has been demonstrated that at high concentrations, NO leads to increases in the production of peroxynitrite and oxidative stress , with consequent DNA damage . Finally, the beneficial pleiotropic effects of sildenafil could also be explained, by pharmacokinetic and pharmacodynamics characteristics: high volume of distribution  and ubiquity of the second-messenger target (cGMP) in cytoprotective signaling pathways  distributed in various tissues/cells expressing PDE5 such as MNC  and liver cells [50–52]. Our findings are in agreement with recent studies in many experimental models, which have demonstrated a protection of MNC  and prevention of liver injury by sildenafil through a direct contribution of the NO/cGMP mechanism [52, 54, 55].
The comet assay used for analyses of DNA is a well-established, simple, sensitive and extensively used tool to evaluate genotoxicity by comet tail parameters that are related to the amount of damage in nuclear DNA . An advantage of this technique is the possibility of its use in different cell types when it is suggested that the genotoxicity is tissue specific [16, 17]. Some authors have suggested that comet pictures might be associated with apoptotic cell nuclei, but they have limited this interpretation to highly damaged cells called “ghost cells” that are easily recognizable in situ on the slides [57, 58]. Previous studies have shown that the apoptosis could influence the parameters of the comet tail, but not enough to distort the interpretation of the results . Additionally, the present study supports the idea that the comet assay is a useful approach to assess the efficacy in the treatment on genotoxicity of MNC and liver cells, even in a short time interval (3 weeks).
The importance of evaluating MNC is based in the fact that atherosclerosis is an inflammatory disease [3, 25] that causes an increase in recruitment of inflammatory leukocytes, mainly the MNC types. Likewise, the liver was focused due to its metabolic importance and to its high susceptibility to cumulative oxidized products of DNA . Furthermore, Folkmann et al.  showed that dyslipidemic apoE-/- mice suffer from hepatic oxidative stress/genotoxicity and this could be due to dysfunction of the lipid metabolism. The novelty of the present study is that it was possible to reduce DNA damage in MNC and liver cells of apoE-/- mice by chronic inhibition of PDE5 with sildenafil, even under conditions of hypercholesterolemia, possibly by the same antioxidative mechanisms above commented. This finding supports the idea that sildenafil is a promising novel pharmacologic strategy to avoid tissue damage induced by oxidative stress as previously reported by us  and others [40, 41, 48], thus opening the way for translational studies about the protection of DNA in different clinical conditions.
Study strength and limitations
A major strength of our study is the evaluation of the beneficial effects of sildenafil on genotoxicity in the apoE-/- mouse, which exhibited a protective action against DNA damage in MNC and liver cells. However, this study has some limitations. Although it has been demonstrated that sildenafil administered for 3 weeks reduces oxidative stress in smoke induced erectile dysfunction in C57BL6 mice  and has been considered a novel therapeutic strategy to repair the endothelial dysfunction in apoE-/- mice , we cannot predict whether such beneficial effects on genotoxicity and oxidative stress are long-lasting in atherosclerosis. Another limitation of the present study is that we did not include in our protocol a group of apoE-/- under a regular chow.
ApoE-/- mice are characterized by a systemic oxidative stress, as demonstrated by MNC high levels of superoxide anion production that leads to DNA damage. In these animals, hepatic oxidative stress (in terms of DNA damage) is also substantial, when compared to control normocholesterolemic animals. The treatment with sildenafil was efficient to decrease the levels of superoxide anion in MNC and the DNA fragmentation in both MNC and liver cells in apoE-/- mice. Thus, we propose that sildenafil may offer a new perspective to the use of PDE5 inhibitors to protect against DNA damage observed in atherosclerosis, independent of hypercholesterolemia.
Experiments were performed in male WT (C57BL6) and apoE-/- mice obtained from the Laboratory of Transgenes in the Health Sciences Center at the Federal University of Espirito Santo, Brazil. Animals were housed in individual plastic cages with a controlled temperature (22-23°C) and humidity (60%) and were exposed to a 12:12-h light–dark cycle. All experimental procedures were performed in accordance with the guidelines for the care and handling of laboratory animals as recommended by the National Institutes of Health (NIH), and study protocols were previously approved by the Institutional Animal Care and Use Committee (CEUA-Emescam, Protocol 007/2010).
To accelerate and aggravate the spontaneous hyperlipidemia and atherosclerosis in apoE-/- mice, 8-week-old animals were fed a Western-type diet (AIN93G modified diet, Rhoster, Brazil). Animals were distributed into three different groups: (a) apoE−/− mice administered with the PDE5 inhibitor sildenafil (apoE−/− sildenafil, Viagra®, 40 mg/kg/day, for 3 weeks, by oral gavage), (b) apoE-/- mice administered with vehicle and (c) WT control mice. This dose of sildenafil has been previously used in apoE-/- mice by Dussault et al.  and by our laboratory  in studies about endothelial dysfunction, based on the fact that this drug has reduced oral bioavailability by pre-systemic hepatic metabolism besides high clearance in mice . All animals had ad libitum access to water and food during housing and treatment periods. For each protocol were used 6 to 10 animals per group.
Animals were euthanized with sodium thiopental overdose (100 mg/kg, IP). A thoracic incision was performed for blood collection trough intra-cardiac puncture. Blood was immediately transferred to a tube containing EDTA. Peripheral blood mononuclear cells (MNC) were isolated by Histopaque® density gradient centrifugation, according to the manufacturer’s instructions. The samples were stored at -80°C until further analysis.
Liver cells enriched fractions from the mice liver of different groups were prepared as standardized in our laboratory based on previous studies [5, 17]. The left lobe of the liver was grossly triturated with surgical scissors and incubated with an extraction solution containing proteinase K (Sigma-Aldrich, St. Louis, MO, USA) and collagenase type II (Gibco Life Technologies, São Paulo, SP, Brazil) to dissociate the cells. Then, the cell extract was filtered through a nylon screen (BD falcon 70 μm) to remove cell debris. After, the samples were washed twice in phosphate-buffered saline (PBS) to remove the enzymes. The samples were stored at -80°C until further analysis.
Before performing analyzes of ROS production and genotoxicity was carried out viability test through trypan blue exclusion test and both MNC and liver cells showed 80–90% viability.
Measurement of lipid profile
The plasma of peripheral blood samples was used to measurement of lipid profile, total plasma cholesterol, HDL, LDL and triglycerides were determined using commercial colorimetric assay kits (Bioclin, Belo Horizonte, Brazil). VLDL and IDL were estimated by subtracting HDL and LDL from total serum cholesterol.
Measurement of cytoplasmic reactive oxygen species by DHE
DHE was used for the flow cytometry detection of intracellular superoxide anion. DHE is freely permeable to cells and is rapidly oxidized, mostly by superoxide, to ethidium, which binds to DNA and amplifies red fluorescence signal. To estimate the content of superoxide anion in cell suspension, 106 MNC were incubated with 20 μL of DHE (160 μM) for 30 min at 37°C in the dark to load the cells with the dyes [11, 35]. For positive control, samples were treated for 5 min with 50 μM H2O2 to create an oxidative stress without being toxic to the cells. Cells were then washed, resuspended in PBS, and kept on ice for an immediate detection by flow cytometry (FACSCanto II, Becton Dickinson, San Juan, CA, USA). Data was acquired and analyzed using the FACSDiva software (Becton Dickinson, San Juan, CA, USA). For quantification of DHE, samples were acquired in triplicate and 10,000 events were used for each measurement. Cells were excited at 488 nm and DHE was detected using 585/42 bandpass filter and data expressed as the median fluorescence intensity (MFI).
Measurement of oxidized DNA by alkaline comet assay
The DNA damage was assessed using alkaline single cell gel electrophoresis (the alkaline comet assay), following established protocols from our laboratory [11, 26, 35] based on Singh et al.  with minor modifications and under low brightness and controlled temperature due to the photo and thermo sensitivity of the assay.
The comet assay is a well validated technique for measurement of DNA damage in individual cells . In brief, histological slides were precoated with 1.5% normal melting point agarose in PBS in a water-bath at 65°C. Subsequently, 20 μL of cell suspension was embedded in 100 μL of 0.5% low melting point agarose in PBS at 37°C and spread on agarose-precoated slides using coverslips. After gelling at 4°C for 20 min, the coverslips were removed and the slides were immersed in freshly prepared lysis solution (2.5 M NaCl, 100 mM EDTA, 10 mM Tris, 34 mM N-Lauroylsarcosine sodium, adjusted to pH 10.0-10.5, using freshly added 1% Triton X-100 and 10% DMSO) for 1 hour at 4°C. After washing in cold distilled water, the slides were quickly immersed in PBS solution. Then, the slides were placed in an electrophoresis chamber filled with freshly prepared alkaline buffer (300 mM NaOH, 1 mM EDTA, pH > 13) for 40 min at 4°C, and electrophoresed at 300 mA and 20 V for 30 min. Afterwards, the slides were neutralized with a 0.4 M Tris buffer (pH 7.5), for 5 min, washed with cold distilled water and allowed to dry at room temperature for 1 hour.
Migration of DNA fragments towards the anode creates a comet ‘tail’, visualized by staining with ethidium bromide (20 μg/mL, Sigma-Aldrich). Immediately afterwards, images were obtained at a magnification of 20x using a fluorescence optical microscope (Nikon Eclipse TI, Melville, NY, USA) equipped with excitation (510-550 nm) and barrier (590 nm) filters. The coded images were acquired using a CCD camera (Nikon) and were analyzed with the CASP program (public domain). Among several parameters provided by the program CASP, we used the percentage of DNA in the tail and the tail moment for analysis of DNA damage. Comets with more than 25% of tail DNA were categorized as moderate-to-high damage and quantified. All samples were coded before scoring. The images of 100 randomly selected cells from each sample obtained from two replicate slides for each animal were analyzed. During the image analysis, comets without clearly identifiable heads or comets with most of their DNA in their tails after electrophoresis were excluded, as a quality control parameter.
All data are expressed as the mean ± SEM. The Kolmogorov-Smirnov test showed that variables had a normal (Gaussian) distribution. The statistical analysis was performed using the one-way analysis of variance (ANOVA). When the ANOVA showed significant differences, the Bonferroni’s test was performed as a post hoc analysis. The differences were considered significant when p < 0.05.
ECV is supported by the National Council for the Development of Science and Technology (CNPq, Ref. 302582/2011-8 and 476525/2012-8 Grants) and the State Agency for the Development of Science and Technology (FAPES/Universal 012/2011/Proc. 54498465). SSM is supported by the National Council for the Development of Science and Technology (CNPq, Ref. 305188/2012-7 Grant) and the State Agency for the Development of Science and Technology (FAPES/CNPq/PRONEX Edital 012/2009).
- Pashkow FJ: Oxidative stress and inflammation in heart disease: do antioxidants have a role in treatment and/or prevention?. Int J Inflam. 2011, 2011: 9-View ArticleGoogle Scholar
- Minamino T, Komuro I: Vascular cell senescence: contribution to atherosclerosis. Circ Res. 2007, 100: 15-26. 10.1161/01.RES.0000256837.40544.4aView ArticlePubMedGoogle Scholar
- Vasquez EC, Peotta VA, Gava AL, Pereira TMC, Meyrelles SS: Cardiac and vascular phenotypes in the apolipoprotein E-deficient mouse. J Biomed Sci. 2012, 19: 22. 10.1186/1423-0127-19-22PubMed CentralView ArticlePubMedGoogle Scholar
- ‘t Hoen PAC, Van der Lans CAC, Van Eck M, Bijsterbosch MK, Van Berkel TJC, Twisk J: Aorta of apoE-deficient mice responds to atherogenic stimuli by a prelesional increase and subsequent decrease in the expression of antioxidant enzymes. Circ Res. 2003, 93: 262-269. 10.1161/01.RES.0000082978.92494.B1View ArticlePubMedGoogle Scholar
- Folkmann JK, Risom L, Hansen CS, Loft S, Møller P: Oxidatively damaged DNA and inflammation in the liver of dyslipidemic ApoE-/- mice exposed to diesel exhaust particles. Toxicology. 2007, 237: 134-144. 10.1016/j.tox.2007.05.009View ArticlePubMedGoogle Scholar
- Vasquez EC, Peotta VA, Meyrelles SS: Cardiovascular autonomic imbalance and baroreflex dysfunction in the apolipoprotein E-deficient mouse. Cell Physiol Biochem. 2012, 29: 635-646. 10.1159/000277623View ArticlePubMedGoogle Scholar
- Esterbauer H: Cytotoxicity and genotoxicity of lipid-oxidation products. Am J Clin Nutr. 1993, 57: 779S-786S.PubMedGoogle Scholar
- Yin H, Porter NA: New insights regarding the autoxidation of polyunsaturated fatty acids. Antioxid Redox Signal. 2005, 7: 170-184. 10.1089/ars.2005.7.170View ArticlePubMedGoogle Scholar
- Fitó M, de la Torre R, Covas MI: Olive oil and oxidative stress. Mol Nutr Food Res. 2007, 51: 1215-1224.PubMedGoogle Scholar
- Leonarduzzi G, Gamba P, Gargiulo S, Biasi F, Poli G: Inflammation-related gene expression by lipid oxidation-derived products in the progression of atherosclerosis. Free Radic Biol Med. 2012, 52: 19-34. 10.1016/j.freeradbiomed.2011.09.031View ArticlePubMedGoogle Scholar
- Tonini CL, Campagnaro BP, Louro LPS, Pereira TMC, Vasquez EC, Meyrelles SS: Effects of aging and hypercholesterolemia on oxidative stress and DNA damage in bone marrow mononuclear cells in apolipoprotein E-deficient mice. Int J Mol Sci. 2013, 14: 3325-3342. 10.3390/ijms14023325PubMed CentralView ArticlePubMedGoogle Scholar
- Milani E, Nikfar S, Khorasani R, Zamani MJ, Abdollahi M: Reduction of diabetes-induced oxidative stress by phosphodiesterase inhibitors in rats. Comp Biochem Physiol C Toxicol Pharmacol. 2005, 140: 251-255.View ArticlePubMedGoogle Scholar
- Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J: Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007, 39: 44-84. 10.1016/j.biocel.2006.07.001View ArticlePubMedGoogle Scholar
- Dizdaroglu M: Chemical determination of free radical-induced damage to DNA. Free Radic Biol Med. 1991, 10: 225-242. 10.1016/0891-5849(91)90080-MView ArticlePubMedGoogle Scholar
- Singh NP, McCoy MT, Tice RR, Schneider EL: A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988, 175: 184-191. 10.1016/0014-4827(88)90265-0View ArticlePubMedGoogle Scholar
- Belpaeme K, Cooreman K, Kirsch-Volders M: Development and validation of the in vivo alkaline comet assay for detecting genomic damage in marine flatfish. Mutat Res. 1998, 415: 167-184. 10.1016/S1383-5718(98)00062-XView ArticlePubMedGoogle Scholar
- Folkmann JK, Loft S, Møller P: Oxidatively damaged DNA in aging dyslipidemic ApoE-/- and wild-type mice. Mutagenesis. 2007, 22: 105-110. 10.1093/mutage/gel059View ArticlePubMedGoogle Scholar
- Riazi K, Roshanpour M, Rafiei-Tabatabaei N, Homayoun H, Ebrahimi F, Dehpour AR: The proconvulsant effect of sildenafil in mice: role of nitric oxide-cGMP pathway. Br J Pharmacol. 2006, 147: 935-943. 10.1038/sj.bjp.0706680PubMed CentralView ArticlePubMedGoogle Scholar
- Ebrahimi F, Shafaroodi H, Asadi S, Nezami BG, Ghasemi M, Rahimpour S, Hashemi M, Doostar Y, Dehpour AR: Sildenafil decreased cardiac cell apoptosis in diabetic mice: reduction of oxidative stress as a possible mechanism. Can J Physiol Pharmacol. 2009, 87: 556-564. 10.1139/Y09-036View ArticlePubMedGoogle Scholar
- Balarini CM, Leal MA, Gomes IBS, Pereira TMC, Gava AL, Meyrelles SS, Vasquez EC: Sildenafil restores endothelial function in the apolipoprotein E knockout mouse. J Transl Med. 2013, 11: 3- 10.1186/1479-5876-11-3PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang SH, Reddick RL, Burkey B, Maeda N: Diet-induced atherosclerosis in mice heterozygous and homozygous for apolipoprotein E gene disruption. J Clin Invest. 1994, 94: 937-945. 10.1172/JCI117460PubMed CentralView ArticlePubMedGoogle Scholar
- Reddick RL, Zhang SH, Maeda N: Atherosclerosis in mice lacking apo E evaluation of lesional development and progression. Arterioscler Thromb. 1994, 14: 141-147. 10.1161/01.ATV.14.1.141View ArticlePubMedGoogle Scholar
- Dussault S, Maingrette F, Ménard C, Michaud S-E, Haddad P, Groleau J, Turgeon J, Perez G, Rivard A: Sildenafil increases endothelial progenitor cell function and improves ischemia-induced neovascularization in hypercholesterolemic apolipoprotein E deficient mice. Hypertension. 2009, 54: 1043-1049. 10.1161/HYPERTENSIONAHA.109.139451View ArticlePubMedGoogle Scholar
- Porto ML, Lima LCF, Pereira TMC, Nogueira BV, Tonini CL, Campagnaro BP, Meyrelles SS, Vasquez EC: Mononuclear cell therapy attenuates atherosclerosis in apoE KO mice. Lipids Health Dis. 2011, 10: 155. 10.1186/1476-511X-10-155PubMed CentralView ArticlePubMedGoogle Scholar
- Meyrelles SS, Peotta VA, Pereira TMC, Vasquez EC: Endothelial dysfunction in the apolipoprotein E-deficient mouse: insights into the influence of diet, gender and aging. Lipids Health Dis. 2011, 10: 211. 10.1186/1476-511X-10-211PubMed CentralView ArticlePubMedGoogle Scholar
- Dalboni SP, Campagnaro BP, Tonini CL, Vasquez EC, Meyrelles SS: The concurrence of hypercholesterolemia and aging promotes DNA damage in apolipoprotein E-deficient mice. Open J Blood Dis. 2012, 02: 51-55. 10.4236/ojbd.2012.23010. 10.4236/ojbd.2012.23010View ArticleGoogle Scholar
- Kojda G, Harrison D: Interactions between NO and reactive oxygen species: pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc Res. 1999, 43: 562-571. 10.1016/S0008-6363(99)00169-8View ArticlePubMedGoogle Scholar
- Duez P, Dehon G, Kumps A, Dubois J: Statistics of the comet assay: a key to discriminate between genotoxic effects. Mutagenesis. 2003, 18: 159-166. 10.1093/mutage/18.2.159View ArticlePubMedGoogle Scholar
- Izzotti A: DNA damage and alterations of gene expression in chronic-degenerative diseases. Acta Biochim Pol. 2003, 50: 145-154.PubMedGoogle Scholar
- Andreassi MG: Coronary atherosclerosis and somatic mutations: an overview of the contributive factors for oxidative DNA damage. Mutat Res. 2003, 543: 67-86. 10.1016/S1383-5742(02)00089-3View ArticlePubMedGoogle Scholar
- Ronsein GE, Oliveira MCB, Medeiros MHG, Miyamoto S, Di Mascio P: DNA strand breaks and base modifications induced by cholesterol hydroperoxides. Free Radic Res. 2011, 45: 266-275. 10.3109/10715762.2010.524215View ArticlePubMedGoogle Scholar
- Yao Y, Tian X, Liu X, Shao J, LV Y: The p53-mediated apoptosis in hypercholesterolemia-induced renal injury of rats. J Huazhong Univ Sci Technolog Med Sci. 2005, 25: 408-411. 10.1007/BF02828209View ArticlePubMedGoogle Scholar
- Sevin G, Yasa M, Akcay DY, Kirkali G, Kerry Z: Different responses of fluvastatin to cholesterol-induced oxidative modifications in rabbits: evidence for preventive effect against DNA damage. Cell Biochem Funct. 2013, 31: 325-332. 10.1002/cbf.2903View ArticlePubMedGoogle Scholar
- Silva BR, Pernomian L, Bendhack LM: Contribution of oxidative stress to endothelial dysfunction in hypertension. Front Physiol. 2012, 3: 441-PubMed CentralView ArticlePubMedGoogle Scholar
- Campagnaro BP, Tonini CL, Nogueira BV, Casarini DE, Vasquez EC, Meyrelles SS: DNA damage and augmented oxidative stress in bone marrow mononuclear cells from angiotensin-dependent hypertensive mice. Int J Hypertens. 2013, 2013: 10-Google Scholar
- Campagnaro BP, Gava AL, Meyrelles SS, Vasquez EC: Cardiaca-autonomic imbalance and baroreflex dysfunction in the renovascular angiotensin-dependent hypertensive mouse. Int J Hypertens. 2012, 2012: 9-Google Scholar
- Srujana K, Begum SS, Rao KN, Devi GS, Jyothy A, Prasad MH: Application of the comet assay for assessment of oxidative DNA damage in circulating lymphocytes of Tetralogy of Fallot patients. Mutat Res. 2010, 688: 62-65. 10.1016/j.mrfmmm.2010.03.009View ArticlePubMedGoogle Scholar
- Andreassi MG, Botto N: DNA damage as a new emerging risk factor in atherosclerosis. Trends Cardiovasc Med. 2003, 13: 270-275. 10.1016/S1050-1738(03)00109-9View ArticlePubMedGoogle Scholar
- Mahmoudi M, Mercer J, Bennett M: DNA damage and repair in atherosclerosis. Cardiovasc Res. 2006, 71: 259-268. 10.1016/j.cardiores.2006.03.002View ArticlePubMedGoogle Scholar
- Koupparis AJ, Jeremy JY, Muzaffar S, Persad R, Shukla N: Sildenafil inhibits the formation of superoxide and the expression of gp47phox NAD[P]H oxidase induced by the thromboxane A2 mimetic, U46619, in corpus cavernosal smooth muscle cells. BJU Int. 2005, 96: 423-427. 10.1111/j.1464-410X.2005.05643.xView ArticlePubMedGoogle Scholar
- Muzaffar S, Shukla N, Srivastava A, Angelini GD, Jeremy JY: Sildenafil citrate and sildenafil nitrate (NCX 911) are potent inhibitors of superoxide formation and gp91phox expression in porcine pulmonary artery endothelial cells. Br J Pharmacol. 2005, 146: 109-117. 10.1038/sj.bjp.0706305PubMed CentralView ArticlePubMedGoogle Scholar
- Vertuani S, Angusti A, Manfredini S: The antioxidants and pro-antioxidants network: an overview. Curr Pharm Des. 2004, 10: 1677-1694. 10.2174/1381612043384655View ArticlePubMedGoogle Scholar
- Ozkanlar S, Akcay F: Antioxidant vitamins in atherosclerosis - animal experiments and clinical studies. Adv Clin Exp Med. 2012, 21: 115-123.PubMedGoogle Scholar
- Colasanti M, Suzuki H: The dual personality of NO. Trends Pharmacol Sci. 2000, 21: 1998-2001.View ArticleGoogle Scholar
- Tousoulis D, Papageorgiou N, Briasoulis A, Androulakis E, Charakida M, Tsiamis E, Stefanadis C: Conflicting effects of nitric oxide and oxidative stress in chronic heart failure: potential therapeutic strategies. Heart Fail Rev. 2012, 17: 65-79. 10.1007/s10741-011-9228-4View ArticlePubMedGoogle Scholar
- Hrabarova E, Juranek I, Soltes L: Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation. Gen Physiol Biophys. 2011, 30: 223-228. 10.4149/gpb_2011_03_223View ArticlePubMedGoogle Scholar
- Nichols DJ, Muirhead GJ, Harness JA: Pharmacokinetics of sildenafil citrate after single oral doses in healthy male subjects: absolute bioavailability, food effects and dose proportionality. Br J Pharmacol. 2002, 53: 5S-12S.View ArticleGoogle Scholar
- Das A, Durrant D, Mitchell C, Mayton E, Hoke NN, Salloum FN, Park MA, Qureshi I, Lee R, Dent P, Kukreja RC: Sildenafil increases chemotherapeutic efficacy of doxorubicin in prostate cancer and ameliorates cardiac dysfunction. Proc Natl Acad Sci U S A. 2010, 107: 18202-18207. 10.1073/pnas.1006965107PubMed CentralView ArticlePubMedGoogle Scholar
- Essayan DM: Cyclic nucleotide phosphodiesterases. J Allergy Clin Immunol. 2001, 108: 671-680. 10.1067/mai.2001.119555View ArticlePubMedGoogle Scholar
- Kulkarni SK, Patil CS: Phosphodiesterase 5 enzyme and its inhibitors: update on pharmacological and therapeutical aspects. Meth Find Exp Clin Pharmacol. 2004, 26: 789-799. 10.1358/mf.2004.26.10.872561.View ArticleGoogle Scholar
- Loureiro-Silva MR, Iwakiri Y, Abraldes JG, Haq O, Groszmann RJ: Increased phosphodiesterase-5 expression is involved in the decreased vasodilator response to nitric oxide in cirrhotic rat livers. J Hepatol. 2006, 44: 886-893. 10.1016/j.jhep.2006.01.032View ArticlePubMedGoogle Scholar
- Lee KC, Yang YY, Huang YT, Lee FY, Hou MC, Lin HC, Lee SD: Administration of a low dose of sildenafil for 1 week decreases intrahepatic resistance in rats with biliary cirrhosis: the role of NO bioavailability. Clin Sci (Lond). 2010, 119: 45-55. 10.1042/CS20090601.View ArticleGoogle Scholar
- Di X, Gennings C, Bear HD, Graham LJ, Sheth CM, White KL, Gewirtz DA: Influence of the phosphodiesterase-5 inhibitor, sildenafil, on sensitivity to chemotherapy in breast tumor cells. Breast Canc Res Treat. 2010, 124: 349-360. 10.1007/s10549-010-0765-7.View ArticleGoogle Scholar
- Yardimci S, Bostanci EB, Ozer I, Dalgic T, Surmelioglu A, Aydog G, Akoglu M: Sildenafil accelerates liver regeneration after partial hepatectomy in rats. Transplant Proc. 2012, 44: 1747-1750. 10.1016/j.transproceed.2012.04.003View ArticlePubMedGoogle Scholar
- Inan M, Uz YH, Kizilay G, Topcu-Tarladacalisir Y, Sapmaz-Metin M, Akpolat M, Aydogdu N: Protective effect of sildenafil on liver injury induced by intestinal ischemia/reperfusion. J Pediatr Surg. 2013, 48: 1707-1715. 10.1016/j.jpedsurg.2012.12.054View ArticlePubMedGoogle Scholar
- Dhawan A, Bajpayee M, Parmar D: Comet assay: a reliable tool for the assessment of DNA damage in different models. Cell Biol Toxicol. 2009, 25: 5-32. 10.1007/s10565-008-9072-zView ArticlePubMedGoogle Scholar
- Godard T, Deslandes E, Lebailly P, Vigreux C, Sichel F, Poul J, Gauduchon P: Early detection of staurosporine-induced apoptosis by comet and annexin V assays. Histochem Cell Biol. 1999, 112: 155-161. 10.1007/s004180050402View ArticlePubMedGoogle Scholar
- Choucroun P, Gillet D, Dorange G, Sawicki B, Dewitte JD: Comet assay and early apoptosis. Mutat Res. 2001, 478: 89-96. 10.1016/S0027-5107(01)00123-3View ArticlePubMedGoogle Scholar
- Bivalacqua TJ, Sussan TE, Gebska MA, Strong TD, Berkowitz DE, Biswal S, Burnett AL, Champion HC: Sildenafil inhibits superoxide formation and prevents endothelial dysfunction in a mouse model of secondhand smoke induced erectile dysfunction. J Urol. 2009, 181: 899-906. 10.1016/j.juro.2008.10.062PubMed CentralView ArticlePubMedGoogle Scholar
- Walker DK, Ackland MJ, James GC, Muirhead GJ, Rance DJ, Wastall P, Wright PA: Pharmacokinetics and metabolism of sildenafil in mouse, rat, rabbit, dog and man. Xenobiotica. 1999, 29: 297-310. 10.1080/004982599238687View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.