Comparing effects of soybean oil- and palm olein-based mayonnaise consumption on the plasma lipid and lipoprotein profiles in human subjects: a double-blind randomized controlled trial with cross-over design
© The Author(s). 2016
Received: 19 May 2016
Accepted: 3 August 2016
Published: 17 August 2016
Mayonnaise is used widely in contemporary human diet with widespread use as a salad dressing or spread on breads. Vegetable oils used in its formulation may be a rich source of ω-6 PUFAs and the higher-PUFA content of mayonnaise may be beneficial in mediating a hypocholesterolemic effect. This study, therefore, evaluated the functionality of mayonnaise on cardiometabolic risk within a regular human consumption scenario.
Subjects underwent a randomized double-blind crossover trial, consuming diets supplemented with 20 g/day of either soybean oil-based mayonnaise (SB-mayo) or palm olein-based mayonnaise (PO-mayo) for 4 weeks each with a 2-week wash-out period. The magnitude of changes for metabolic outcomes between dietary treatments was compared with PO-mayo serving as the control. The data was analyzed by ANCOVA using the GLM model. Analysis was adjusted for weight changes.
Treatments resulted in significant reductions in TC (diff = −0.25 mmol/L; P = 0.001), LDL-C (diff = −0.17 mmol/L; P = 0.016) and HDL-C (diff = −0.12 mmol/L; P < 0.001) in SB-mayo compared to PO-mayo without affecting LDL-C:HDL-C ratio (P > 0.05). Lipoprotein particle change was significant with large LDL particles increasing after PO-mayo (diff = +63.2 nmol/L; P = 0.007) compared to SB-mayo but small LDL particles remained unaffected. Plasma glucose, apolipoproteins and oxidative stress markers remained unchanged.
Daily use with 20 g of linoleic acid-rich SB-mayo elicited reductions in TC and LDL-C concentrations without significantly changing LDL-C:HDL-C ratio or small LDL particle distributions compared to the PO-mayo diet.
This clinical trial was retrospectively registered with the National Medical Research Register, National Institute of Health, Ministry of Health Malaysia, (NMRR-15-40-24035; registered on 29/01/2015; https://www.nmrr.gov.my/fwbPage.jsp?fwbPageId=ResearchISRForm&fwbAction=Update&fwbStep=10&pk.researchID=24035&fwbVMenu=3&fwbResearchAction=Update). Ethical approval was obtained from the National University of Malaysia’s Medical Ethics Committee (UKM 220.127.116.11/244/SPP/NN-054-2011, approved on 25/05/2011).
Therapeutic lifestyle changes that address dietary correction are core principles to prevent chronic disease burden. The United States Dietary Guidelines (USDG) Technical Report in 2015  dropped dietary cholesterol from the list of ‘nutrients of concern’ in relation to the chronic disease burden. This update is in concordance with the lack of evidence from clinical studies showing a benefit in dietary cholesterol reduction . Instead, the USDG Technical Report has emphasized substituting saturated fats with polyunsaturated fatty acid (PUFA) alternatives as part of healthy dietary recommendations . Based on controlled feeding trials, the strength of evidence is rated as strong for every 1 % of energy from saturated fatty acid (SFA) that is replaced with PUFA which produces a 1.8 mg/dL decrease in low density lipoprotein-cholesterol (LDL-C) . The consumption of PUFA-enriched foods, mainly from the ω-6 family, is therefore necessary in order to make this recommendation actionable. The relationship between SFA and PUFA intakes in general populations, shows that lower intakes of SFA are not accompanied by higher intakes of PUFA, as is recommended for preventing coronary heart disease . In this regard, the nutraceutical actions of functional food ingredients are been explored as either adjuvant or alternate therapy to pharmacological interventions .
PUFA availability in the human diet is further differentiated into ω-3 and ω-6 fatty acid classes. The ω-3 PUFAs are involved in prostaglandin E3, prostaglandin I3 and thromboxane A3 production while ω-6 PUFAs are involved in the production of prostaglandin E2, prostaglandin I2 and thromboxane A2; and these two fatty acid classes are known to exhibit antagonistic effects. Looking at effects on serum lipids, it has been reported that ω-3 PUFAs lower triglycerides thereby benefiting dyslipidemia management whereas ω-6 PUFAs, specifically linoleic acid, cause decreased LDL-C concentrations . Global data on country-specific ω-6 consumption ranges between 1.2 and 12.5 % of energy, with a global mean of 5.9 % energy . Therefore, in practice, there is dietary insufficiency of ω-6 PUFA in the human diet in most countries, and the recommendation to increase PUFA for cardiovascular disease (CVD) prevention requires some degree of dietary supplementation. In contrast, although ω-3 PUFAs have a secondary prevention role in CVD mortality, it is ω-6 PUFA that is perceived to have an etiological effect as a replacement for SFA in reducing LDL-C . A long-term prospective cohort running for 32 years showed ω-6 PUFA intake, especially linoleic acid, was inversely associated with mortality from major CVD causes .
Mayonnaise is a mainstay in the contemporary human diet with widespread use as a salad dressing in the home or restaurant. Traditional mayonnaise is a source of ω-6 PUFAs depending on the source of compositional oils which may be from soybean, canola, corn or sunflower sources . The functional role of fat in mayonnaise formulation is in maintaining food quality related to texture, flavor, and stability of food emulsion products . The PUFA content of mayonnaise ranges between 28.0 and 47.9 g/100 g product . Most regular mayonnaise products produced commercially are formulated with soybean oil (SB). SB contains a significant proportion of linoleic acid (LA, 18:2 > 50 %) and less palmitic acid (16:0 < 10 %) and being PUFA-rich would be expected to contribute to a hypocholesterolemic effect . However if this is true, no literature has reported on the cholesterolemic lowering effect of mayonnaise if consumed consistently over a period of time. On the other hand, Amini et al. , who conducted a cross-sectional examination of adult Iranian diets (n = 425), suggested mayonnaise as part of an undesirable Western-pattern diet matrix was associated with risk of metabolic syndrome (MetS). This view differs from a Japanese study which in examining associations between major dietary patterns and MetS prevalence, reported mayonnaise was not associated with prevalence of MetS, but was positively associated with high blood glucose .
Although, coronary risk is assessed in terms of atherosclerosis development , the wider scope today includes MetS, with inflammation and oxidative stress implicated in its early pathology [14–16]. Our key research question therefore was- given the fatty acid compositional profile of mayonnaise if consumed on a daily basis- what would be its direct impact on lipid and lipoprotein profile? On a wider scope, what would be the impact on other metabolic parameters related to oxidative stress and inflammation? An additional interest in this study was to also address an issue whether subclass differences in LDL and high density lipoprotein (HDL) particle profiles were responsive to the manipulated linoleic acid content of the total diet.
Overall (n = 34)
23.4 ± 7.0
Body weight (kg)
64.5 ± 14.4
25.1 ± 4.7
5.01 ± 0.54
1.39 ± 0.30
3.18 ± 0.48
0.99 ± 0.46
4.70 ± 0.44
This study was conducted in accordance with the principles of the Declaration of Helsinki with ethical approval provided by the National University of Malaysia’s Medical Ethics Committee (UKM 18.104.22.168/244/SPP/NN-054-2011 approved on 25/05/2011).
Test mayonnaise products
Percent fatty acid composition (%) of mayonnaise products, duplicated diets and plasma triacylglycerol
Fatty acids (%)
Duplicated total diets b
0.37 ± 0.29
0.51 ± 0.41
0.43 ± 0.06
0.49 ± 0.35
0.36 ± 0.64
0.79 ± 0.12
0.95 ± 0.35
1.57 ± 0.70
1.14 ± 0.47
0.99 ± 0.41
28.0 ± 2.9*
34.6 ± 1.7*
32.1 ± 2.3
29.6 ± 1.7*
31.2 ± 1.7*
0.55 ± 0.12
0.62 ± 0.17
4.09 ± 0.81
4.26 ± 0.87
4.21 ± 1.11
4.40 ± 0.93
4.23 ± 0.52
4.09 ± 1.17
3.16 ± 0.64
3.08 ± 0.52
37.5 ± 1.2*
41.8 ± 1.7*
40.9 ± 2.3
40.9 ± 2.3*
43.7 ± 1.7*
23.4 ± 3.5*
13.0 ± 1.7*
12.0 ± 1.7
15.3 ± 1.2*
12.7 ± 1.7*
0.18 ± 0.12*
0.04 ± 0.35*
0.24 ± 0.12
0.20 ± 0.12
0.15 ± 0.06
1.05 ± 0.41
0.77 ± 0.87
0.30 ± 0.12
0.35 ± 0.12
0.26 ± 0.17
0.10 ± 0.58
0.15 ± 0.93
0.57 ± 1.98
0.62 ± 0.23
0.59 ± 0.23
35.1 ± 2.8*
41.9 ± 1.3*
37.9 ± 3.3
34.3 ± 2.4*
35.5 ± 2.2*
38.5 ± 1.3*
42.8 ± 1.8*
45.0 ± 2.5
45.1 ± 2.6*
47.9 ± 2.0*
25.7 ± 3.6*
14.4 ± 0.9*
12.9 ± 2.3
16.3 ± 1.4*
13.4 ± 1.9*
24.0 ± 3.7*
13.2 ± 1.0*
12.7 ± 2.2
15.5 ± 3.1*
13.1 ± 1.8*
1.05 ± 0.41
0.77 ± 0.87
0.28 ± 0.14
0.35 ± 0.13
0.26 ± 0.15
26.7 ± 11.9
24.2 ± 9.6
48.0 ± 14.2
55.7 ± 31.5
71.4 ± 71.4
As study subjects were residing on campus in the study site, all meals inclusive of breakfast, lunch, tea and dinner for each fat rotation were provided by the institutional caterer as instructed by the research dietitian. Subjects were expected to consume these meals at a designated residential cafeteria supervised by the dietitian. A standardized 7-day cycle menu planned with a fixed daily total fat and cholesterol content was used for the two 4-week test rotations. All meals were cooked with palm olein to fix the background fat for meal preparation and were standardized to recipes, portion sizes and procedures for incorporating the cooking oil. The amount of cooking oil used was enough to maintain a reduced fat background (~25 %-en) allowing subjects in the 2 test rotations ~45 g per day of palm olein whilst ~15 g of fat was derived from the test mayonnaise during the feeding trial. Other sources of SFAs such as lauric and myristic acids were limited in the menu by excluding coconut-based products and full-fat dairy products. Skim milk and a limit of 2 eggs per week were allowed. The daily diets provided a total of 62–64 g of fat; the SB-mayo diet carrying 12 g of linoleic acid (~1/3rd of total fat) from the mayonnaise yielded final concentrations of 23.4 % 18:2ω6 and 1.05 % 18:3ω3 vs 13.0 % 18:2ω6 and 0.77 % 18:3ω3 in the PO-mayo based diet. Thus for the total diet comparisons, the total ω6 was significantly higher in SB-mayo compared to PO-mayo treatment. Subjects were instructed to eat according to their usual capacity for food intake as determined by energy intake assessment from 24-h diet records at baseline. This pre-study energy intake assessment provided a guideline to the dietitian for ensuring monitoring subjects’ weight fluctuations did not exceed 1 kg within each 4-week test rotation.
For each rotation these included [i] meal time attendance records [ii] weekly recording of body weights of subjects [iii] collecting 3 × 24-h diet records per rotation from every subject and [iv] preparing duplicated diets of all foods consumed over a 24-h period including snacks and fluids except water from subjects for FAC analysis. The 24-h diet records were analyzed with NutriPro software [version 3.2.0] which includes databases for Malaysian foods .
Blood collection and analyses
All subjects arrived at the laboratory after an overnight fasted state (~10 h) on testing days at baseline, day 27 and day 28 of each test rotation. Blood was collected into Vacutainer® tubes (Becton Dickinson Vacutainer, NJ, USA) containing EDTA (0.117 ml of 15 % EDTA) and immediately centrifuged at 3000 g for 10 min at 4 °C (Sigma 3 K12 B.Braun, Germany) to separate plasma from red blood cells. Plasma aliquots were snap-frozen in liquid nitrogen and stored at −80 °C for analyses after study completion. To reduce intra-variations in the analysis, plasma samples were analyzed in a single batch.
Analyses of lipids and lipoproteins
Analyses were carried out using automated clinical chemistry procedures. Enzymatic kits were used to measure plasma TC, TAG and HDL-C (Roche Diagnostics Corporation, Indianapolis, IN, USA). For the determination of cholesterol and TAG concentrations the assay procedures were based on Allain et al. (1974) and Nägele et al. (1984), respectively [21, 22]. The Cobas Integra HDL-Cl plus 2nd generation in vitro diagnostic kit, for the direct determination of HDL-C, combines polyethylene glycol-modified enzymes with sulphated α-cyclodextrins . These assays were performed using a Cobas 6000 Chemistry Autoanalyzer System (Roche Analytic Instruments Inc, Nutley, NJ, USA). Reagents, calibrators and controls were also supplied by the manufacturer (Roche Diagnostics Corporation, Indianapolis, IN, USA). LDL-C was calculated using the Friedewald equation . Very low density lipoprotein-cholesterol (VLDL-C) was calculated by multiplying TAG values by a factor of 0.46.
Determination of glucose
Plasma glucose was assessed by the hexokinase –glucose-6-phosphate dehydrogenase 2-step enzymatic procedure . An in vitro diagnostic reagent kit for this enzymatic procedure, Glucose HK Liquid (GLUCL 0–992) (Roche Diagnostics Corporation, Indianapolis, IN, USA) was used for automated analysis (Roche Analytic Instruments Inc, Nutley, NJ, USA).
Determination of non-esterified fatty acids (NEFA)
Plasma NEFA was assessed by the acyl-CoA synthase-acyl CoA oxidase-peroxidase (ACS-ACOD) 3-step enzymatic procedure  using a commercial kit, Lab Assay™ NEFA (Wako pure chemical industries Ltd., Osaka, Japan) with absorbance detected at 550 nm by a multi-detection microplate reader (Powerscan® HT, DS Pharma Biomedical Co. Ltd., Osaka, Japan).
Determination of apolipoproteins (apo) A1 and B
Plasma apolipoproteins were assessed by the sandwich ELISA procedure using anti-human apoA1 or apoB monoclonal antibody, and horseradish peroxidase-labeled anti-human apoA1 or apoB monoclonal antibody, respectively . A commercial kit, ELISApro kit for Human ApoA1, or ApoB [Mabtech Inc., OH, USA] was used with absorbance detected at 450 nm by a multi-detection microplate reader [Powerscan® HT, DS Pharma Biomedical Co. Ltd., Osaka, Japan].
Determination of thiobarbituric acid reactive substances (T-BARs)
T-BARs was assessed by the malondialdehyde-TBA adduct formed by the reaction of malondialdehyde and T-BARs  under high temperature (90–100 °C) using a commercial kit, T-BARs assay kit (Cayman Chemical Company, MI, USA). Acidic conditions were detected by measuring absorbance at 550 nm with a multi-detection Microplate reader (Powerscan® HT, DS Pharma Biomedical Co. Ltd., Osaka, Japan).
Determination of high sensitivity C-reactive protein (hsCRP)
Plasma hsCRP was assessed by a sandwich ELISA protocol with a commercial kit (Helica Biosystems Inc., CA, USA) which uses anti-human CRP monoclonal antibody, and horseradish peroxidase-labeled anti-human CRP monoclonal antibody . Absorbance at 450 nm was detected by a multi-detection microplate reader (Powerscan® HT, DS Pharma Biomedical Co. Ltd., Osaka, Japan).
NMR lipoprotein particle measures
Particle count and particle size profile determinations for LDL and HDL were carried out by automated nuclear magnetic resonance (NMR) spectroscopic assay by an independent laboratory (LipoScience Inc., Raleigh, North Carolina, USA). EDTA plasma samples held at −80 °C at study conclusion were shipped out in dry ice to the certified laboratory (CLIA ID No. 34D0952253) as per service provider instructions. The NMR technology streamlines the time-consuming ultracentrifugation process into a 2-step analytical process using automated NMR signal acquisition followed by computational analysis .
Analyses of fatty acid composition
Test mayonnaise, duplicated diets and total plasma were subjected to FAC analysis using methodology as described previously [17–19]. FAC analysis was carried out by means of gas chromatography (Shimadzu Scientific Instruments, Japan). The fatty acid profile of analyzed meals served to check whether research diets achieved fatty acid targets. FAC determination of plasma TAG served to check subjects’ compliance to targeted mayonnaise consumption in each test fat rotation as per research protocol.
A cross-over design was used which enabled subjects to serve as their own control. For each subject, the mean value for day 27 and day 28 in each treatment rotation was taken as the end point. In descriptive statistics, data were expressed as mean ± SD and the level of significance was set as P < 0.05. We deduced that the 2-week washout period between treatments was sufficient by comparing values for the 2 baselines by paired sample t-test analysis and found a lack of significance (P > 0.05). Within and between treatment comparisons of targeted fatty acids in plasma triglycerides expected to be reflective of mayonnaise treatment were analyzed by ANCOVA analysis using GLM model. Weight change between treatment periods was analyzed for statistical significance on metabolic outcomes. However, all analysis to test difference in means for metabolic outcomes were carried out controlled for weight changes by ANCOVA procedure using GLM model. The magnitude of changes for metabolic outcomes between dietary treatments was compared with PO-mayo serving as the control for comparison and data was analyzed by ANCOVA using the GLM model. The Statistical Package for Social Sciences, SPSS® for Windows™ application (Version 18.0, SPSS Inc., Chicago, USA) was used for all analyses.
Measures of compliancy
Weight reductions occurred for both rotations (SB-mayo = −0.6 ± 1.1 kg, P = 0.003 and PO-mayo = − 0.9 kg ± 1.1 kg, P < 0.001) compared to baseline. However, mean weight loss at the end of the study periods was less than 1 kg and not significantly different between groups (P > 0.05). This weight change did not influence metabolic outcomes or lipoprotein particle size distributions (P > 0.05).
FAC analysis of diets and plasma TAG
Targeted palmitic, oleic, linoleic and linolenic acids of duplicated diets collected from subjects during each test rotation were significantly different (P < 0.001) between SB-mayo and PO-mayo diets (Table 2). Similarly, consumption of these diets reflected in within- and between-diet effects specific to palmitic, oleic and linoleic acids that were significantly different (P < 0.05) as indicated by FAC analysis of plasma TAG (Table 2). No difference in arachidonic acid (%) in plasma TAG was apparent between dietary treatments (P > 0.05).
Nutrient composition of consumed diets per day
Dietary recalls a
1938 ± 279
1994 ± 356
57.6 ± 11.7
58.9 ± 14.0
284 ± 52
293 ± 64
62.2 ± 8.2
64.1 ± 9.9
147 ± 41
151 ± 41
7.8 ± 1.4*
10.9 ± 1.8*
9.4 ± 1.2*
11.6 ± 1.8*
12.0 ± 2.2*
6.8 ± 1.8*
6.27 ± 1.63
6.58 ± 2.86
SB-mayo treatment significantly reduced TC compared to the PO-mayo diet (diff = −0.25 ± 0.40 mmol/L; P = 0.001).
LDL-C reduction was significantly greater after the SB-mayo treatment compared to the PO-mayo treatment (diff = −0.17 ± 0.40 mmol/L; P = 0.016).
Reduction in HDL-C concentration was associated with the SB-mayo dietary treatment which was significantly lower from the PO-mayo treatment (diff = −0.12 ± 0.15 mmol/L; P < 0.001).
Changes in LDL-C:HDL-C ratio between diets were not significant (P > 0.05).
Plasma TAG, VLDL-C and glucose changes between dietary treatments were not significant (P > 0.05).
A non-significant decreasing effect of SB-mayo treatment on apoB concentrations (diff = −5.86 ± 18.26 mg/mL; P = 0.07) was noted compared to PO-mayo treatment, whereas apoA1 levels were not affected (P > 0.05).
Metabolic parameters in response to mayonnaise diets
Difference between diets
P- time effect (SB-mayo)
P- time effect (PO-mayo)
P- diet differences (ES)
5.20 ± 0.56
4.80 ± 0.65
5.06 ± 0.61
−0.25 ± 0.40
3.37 ± 0.52
3.05 ± 0.57
3.22 ± 0.57
−0.17 ± 0.40
1.39 ± 0.27
1.21 ± 0.27
1.34 ± 0.30
−0.12 ± 0.15
0.94 ± 0.36
1.13 ± 0.54
1.08 ± 0.45
0.05 ± 0.28
0.51 ± 0.19
0.61 ± 0.29
0.58 ± 0.25
0.03 ± 0.15
2.54 ± 0.72
2.67 ± 0.87
2.56 ± 0.80
0.12 ± 0.41
138 ± 20
126 ± 19
124 ± 19
2.00 ± 22.31
119 ± 23
111 ± 26
117 ± 25
−5.86 ± 18.26
4.76 ± 0.37
4.92 ± 0.32
4.95 ± 0.30
−0.04 ± 0.30
0.54 ± 0.19
0.49 ± 0.17
0.49 ± 0.15
0.00 ± 0.15
1.19 ± 0.77
1.22 ± 0.79
1.15 ± 0.66
0.06 ± 0.53
0.22 ± 0.28
0.16 ± 0.21
0.19 ± 0.21
−0.03 ± 0.28
Lipoprotein particle measures
Lipoprotein particle count and lipoprotein particle size distribution as per dietary treatment
Difference between diets
P time effect (SB-mayo)
P time effect (PO-mayo)
P- diet differences (ES)
LDL particles (nmol/L)
1165 ± 318
1094 ± 327
1129 ± 310
−35.0 ± 169.4
574 ± 137
500 ± 151
563 ± 143
−63.2 ± 122.2
48.4 ± 31.7
27.5 ± 33.5
37.0 ± 26.0
−9.50 ± 24.61
543 ± 363
567 ± 377
530 ± 372
37.7 ± 227.8
Mean LDL particle size (nm)
21.6 ± 0.70
21.4 ± 0.77
21.6 ± 0.71
−0.15 ± 0.48
HDL particles (μmol/L)
31.2 ± 3.71
28.7 ± 3.62
29.4 ± 3.28
−0.67 ± 2.56
8.5 ± 2.98
7.8 ± 2.77
7.98 ± 2.66
−0.16 ± 1.45
3.25 ± 2.09
2.46 ± 1.65
2.42 ± 2.42
0.04 ± 2.02
19.5 ± 4.24
18.4 ± 4.06
19.0 ± 3.80
−0.56 ± 3.10
Mean HDL particle size (nm)
9.2 ± 0.46
9.2 ± 0.44
9.2 ± 0.47
−0.05 ± 0.19
However, dietary treatment effect was associated with LDL particle size distributions specific to intermediate LDL (IL) and large LDL (LL) without any change (P > 0.05) in total LDL particle count (TLP). Overall, significant reductions in LL (diff = −63.2 ± 122.2 nmol/L, P = 0.007) and IL (diff = −9.50 ± 24.61 nmol/L, P = 0.040) particles occurred as a result of SB-mayo treatment compared to PO-mayo treatment. Treatment effects on small LDL (SL) were not apparent. Alteration in LDL subclass composition however did not result in differences in mean LDL particle size due to treatments.
Markers of oxidative stress
NEFA, T-BARs and hsCRP outcomes were not significantly different (P > 0.05) between dietary treatments.
The 20 g exchange of test mayonnaise effectively reflected either linoleic (18:2) or palmitic (16:0) acid availability with dietary cholesterol equal between both diets. As intentioned, plasma fatty acid composition reflected higher percentages of palmitic and oleic acids (18:1) when subjects consumed PO-mayo or a linoleic acid (18:2, LA) with the SB-mayo.
Significant reductions in TC and LDL-C concentrations were associated with the SB-mayo rotation compared to the control mayonnaise. It was possible that these reductions were attributed to the higher LA availability from the SB-mayo as it is well established that LA reduces plasma cholesterol concentrations [31, 32]. Concomitant with this approach, it is said that diets lower in SFA and greater in PUFA content result in better CVD outcomes [1, 32, 33]. Substitution with PUFA for SFA was shown to lower CVD risk in the Medical Research Council study , where a high PUFA-rich SB feeding (20.4 %-en) compared to SFA as control (PUFA at 4.4 % - en) resulted in reduced number of cardiac events (rr = 0.86, 95 % CI 0.61, 1.22). The Finnish Mental Hospital Study reported lower mortality when SB replaced regular SFA-containing dairy products in both men (rr = 0.55, 95 % CI 0.34, 0.88)  and women (rr = 0.64, 95 % CI 0.41, 1.00) . These were intervention trials running for 6 years with PUFA provided at 12.9 % of total calories compared to its content in the control diet at 4.3 % of total calories in the present study. The St. Thomas’ Atherosclerosis Regression Study reported a displacement of SFA with PUFA resulted in reversing atherosclerosis with the greatest regression in those reporting consumption of the least amount of SFA . The Atherosclerosis Risk in Communities Study indicated the highest quartile of circulating cholesteryl linoleate after the LA-enriched diet was associated with lower carotid artery intimal medial thickness .
Subjects on the PO-mayo nevertheless experienced a small reducing effect of TC and LDL-C from baseline which could be attributed to its higher oleic acid (18:1) content. Indeed percent oleic acid in plasma after completing the PO-mayo phase was greater (by ~3 %) compared to baseline or during the SB-mayonnaise rotation. MUFA coexists with SFA in many foods which would be true as indicated by the fatty acid composition of palm olein which contains on average 45 % of oleic acid and 39 % of palmitic acid compared to 22 and 11 % respectively for these fatty acids in SB . Additionally, comparison of palm olein and olive oil in human feeding trials indicate that dietary palmitic and oleic acids exert similar effects on plasma lipoproteins [39, 40]. Reduction in dietary SFA intake is also associated with a fall in HDL-C concentrations depending on the substituting nutrient exchange . LA’s additional role in lipoprotein metabolism is in mediating a reducing effect on HDL-C concentrations . We observed this effect when subjects were consuming the LA-rich SB-mayo but not with the PO-mayo. However, LDL-C:HDL-C ratios were not affected. We also noted this HDL-C reduction did not affect total HDL lipoprotein particle number or size distributions.
The LA-enriched diet that included the SB-mayo did not result in an expected significant decrease in apoB concentrations. ApoB is a surrogate measure for atherosclerotic lipoprotein particles as more than 90 % of apoB is carried by LDL particles . LDL lipoprotein subclass distribution is directly implicated in atherogenesis with reduced large buoyant LDL particles and increased small dense LDL particles favouring atherosclerotic plaque deposition [42, 43]. We investigated LDL lipoprotein particle distribution and found an overall 3 % decrease in total LDL particle translated into significant 19.6 % decrease in intermediate LDL particles and also an 11 % decrease in large LDL particles, without a significant change in small LDL particles when subjects were regularly consuming the SB-mayo. Previous interventions have reported standard  or high dose dietary cholesterol  feeding did not cause shifts in LDL particle size irrespective of cholesterolemic status. Thus it is probable that the shifts in LDL lipoprotein particle distributions observed in our study could be attributed to the enriched-LA diet which was exchanged for palmitic acid.
A major concern with higher LA consumption is the hypothesis it could promote an inflammatory environment central to chronic disease development . One pathway is LA-induced arachidonic acid (20:4ω6) enrichment in tissue phospholipids would lead to excessive generation of pro-inflammatory eicosanoids [46, 47]. A systematic review examining Western-type diets did not find, after dietary manipulation, changes in plasma or serum arachidonic acid levels despite a 6-fold increase in dietary LA consumption or up to 90 % reduction in LA . Our study too did not find this effect. Another systematic review of randomized controlled trials, assessing addition of LA to the diet of healthy non-infant populations, did not find any evidence that LA increased the concentration of biologic markers of chronic inflammation including CRP, fibrinogen, plasminogen activator inhibitor type 1, cytokines, soluble vascular adhesion molecules, or tumour necrosis factor-α . We included only hsCRP as an inflammatory marker in our study but this marker was not significantly affected after consumption of the LA-rich diet enriched by the SB-mayo addition.
The capacity of mayonnaise to be conducive in the development of MetS through the inflammatory environment has been suggested . We did not find any significant cause-effect changes in plasma glucose levels or oxidative stress parameters of exogenous origin such as NEFA and T-BARs in response to the dietary manipulation supplementing 20 g of mayonnaise consumption daily of either SB- or PO-based formulations. Our findings are not in agreement with the observations of Akter et al. , who reported mayonnaise consumption, was positively associated with high blood glucose in an adult free living Japanese population with MetS. This difference may in part be explained by the normoglycemic status of our subjects when recruited into the trial.
Donovan and Shamir  view the human diet as a complex food matrix rather than specific nutrients, and highlight a need to resolve how to term a food’s quality or necessity judged as part of an individual’s whole diet. Citing yogurt as an example, they defined its potential as a functional food to manage lactose intolerance or bone health, despite concerns about its high SFA content, which is implicated in the promotion of heart disease. The potential for mayonnaise to be a vehicle for nutraceutical delivery has been explored with diacylglycerol in an anti-obesity function or with plant sterol esters for decreasing LDL-C [50, 51]. Saito et al.  who examined the dose-dependent effect of a mayonnaise-type product combining diacylglycerol with plant sterol esters in subjects with mild hypercholesterolemia found daily ingestion of 15 g of diacylglycerol plus mayonnaise containing at least 0.4 g/d of plant sterol esters for 4 weeks significantly decreased serum cholesterol levels. This load of mayonnaise was similar to the planned intervention in our study.
Results of our study suggest that the active component of SB-mayo is linoleic acid which was observed to lower plasma LDL-C levels. Currently, mayonnaise is a condiment that is commercially available as a general food product and excessive consumption could lead to increased lipid intake which would be counterproductive in terms of excess calories. As scientific proof is necessary to establish the utilization of mayonnaise as a nutraceutical, further research is necessary to determine the appropriate serving size of a mayonnaise product that could lower serum LDL-C levels.
The public health approach for heart health stresses a ~50 % reduction in SFAs to effect a reduction in LDL-C as a means to decrease risk of CVD . Quantitatively, lowering SFAs by 50 % requires substitution with food sources of ω-6 and ω–3 PUFAs and MUFAs [1, 4, 33]. Weighing the emerging nutrition science on fatty acids and CVD, Kris-Etherton and Fleming  acknowledge the best evidence for cardioprotective benefits from substitution of SFAs remains with ω–6 PUFAs, as evidence for MUFAs is mixed whilst only relatively low amounts of ω-3 PUFAs can be incorporated in the diet, even with very high dose. Through this study we have provided evidence that an LA-rich mayonnaise is a convenient vehicle for nutraceutical delivery of the ω-6 PUFA, linoleic acid, therefore expecting a functional use in reduction of LDL-C as a means to decrease risk of CVD. The extrapolation of this study findings may be limited to the general free living population because of the small number of subjects (n = 34) who were relatively young and required to consume research diets provided in an institutional setting.
We demonstrated a functional role for an SB-mayo which was rich in linoleic acid. Uptake of linoleic acid was enhanced, accompanied by a demonstration of the biological activities of increased ω-6 PUFA linoleate. This was also achieved without detrimental shifts in the overall plasma oxidative status of the subjects. These outcomes suggest that the inclusion of about 20 g linoleic acid -rich mayonnaise could be managed as part of a daily healthy diet given the current modern lifestyles that often dictate consumption of prepared foods on the go.
Apo, apolipoprotein; FAC, fatty acid composition; HDL-C, high density lipoprotein cholesterol; hsCRP, high sensitivity C-reactive protein; LA, linoleic acid; LDL-C, low density lipoprotein cholesterol; MetS, metabolic syndrome; MUFA, mono unsaturated fatty acid; NEFA, non-esterified fatty acids; NMR, nuclear magnetic resonance; PO, palm olein; PUFA, poly unsaturated fatty acid; SB, soybean oil; SFA, saturated fatty acid; T-BARs, thiobarbituric acid reactive substances; TC, total cholesterol; TG, triglycerides; VLDL, very low density lipoprotein cholesterol. Lipoprotein particles: TL, total particle count for LDL; IL, intermediate LDL; LL, large LDL and SL, small LDL particle sizes; TH, total particle count for HDL; HL, large HDL; HM, medium HDL and HS, small HDL particle size
The Director of Institut Perguruan Teknik (IPT) and his staff are sincerely acknowledged for extending their support and assistance in ensuring the feeding trials could be carried out smoothly at their facility. We thank Sharmela Sahathevan for serving as the study controller and dietitian, while Tan Choon Heen provided technical support during the feeding rotations.
This study was supported by a grant (NN-188-2010) received from Kewpie Corporation, Japan.
Availability of data and materials
The datasets for this current study is available from the corresponding author on reasonable request.
TK, KS, RM, YM and MS conceived the study concept and design. TK as principal investigator was responsible for study logistics, data acquisition and manuscript preparation. KAC as a PhD scholar was responsible for coordinating the trial, data collection and performing laboratory analysis. KC and KAC carried out the statistical analysis. All authors contributed to the intellectual content of the manuscript. All authors read and approved the final manuscript.
TK, KAC, KS, KC and MS declare no conflict of interest. RM and YM were research scientists at Kewpie Corporation, Japan during the conduct of this trial.
Ethical approval and consent to participate
This study was approved by the National University of Malaysia’s Medical Ethics Committee (UKM 22.214.171.124/244/SPP/NN-054-2011). Informed consent was obtained from all subjects prior to enrolment into study. We are not reporting individual subject data.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- United States Department of Agriculture. Dietary Guidelines for Americans. 2015, USDA-Department of Health and Human Sciences http://www.health.gov/dietaryguidelines/2015-scientific-report. Accessed 1 Aug 2015.
- Shin JY, Xun PC, Nakamura Y, He K. Egg consumption in relation to risk of cardiovascular disease and diabetes: a systematic review and meta-analysis. Am J Clin Nutr. 2013;98:146–59.View ArticlePubMedPubMed CentralGoogle Scholar
- Eckel RH, Jakicic JM, Ard JD, Miller NH, Hubbard VS, Nonas CA, et al. 2013 AHA/ACC Guideline on Lifestyle Management to Reduce Cardiovascular Risk. A Report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines. J Am Coll Cardiol. 2014;63:2960–84.View ArticlePubMedGoogle Scholar
- Harika RK, Eilander A, Alssema M, Osendarp SJ, Zock PL. Intake of fatty acids in general populations worldwide does not meet dietary recommendations to prevent coronary heart disease: a systematic review of data from 40 countries. Ann Nutr Metab. 2013;63:229–38.View ArticlePubMedGoogle Scholar
- Scicchitano P, Cameli M, Maiello M, Amedeo P, Myiesan ML, Novo S, Palmiero P, Saba PS, Pedrinelli R, Ciccone MM. Neurraceuticals and dyslipidemia: beyond the common therapeutics. J Func Foods. 2014;6:11–32.View ArticleGoogle Scholar
- Micha R, Khatibzadeh S, Shi P, Fahimi S, Lim S, Andrews KG, Engell RE, Powles J, Ezzati M, Mozaffarian D, et al. Global, regional, and national consumption levels of dietary fats and oils in 1990 and 2010: a systematic analysis including 266 country-specific nutrition surveys. BMJ. 2014;348:g2272.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang DD, Li Y, Chiuve SE, Stampfer MJ, Manson JE, Rimm EB, Willet WC & Hu FB. Association of specific dietary fats with total and cause-specific mortality. JAMA Intern Med. 2016. doi: 10.1001/jamainternmed.2016.2417.
- Indahl UG, Sahni NS, Kirkhus B, Næs T. Multivariate strategies for classification based on NIR-spectra - with application to mayonnaise. Chemom Intell Lab Syst. 1999;49:19–31.View ArticleGoogle Scholar
- Ma Z, Boye JI. Advances in the design and production of reduced-fat and reduced-cholesterol salad dressing and mayonnaise: a review. Food Bioprocess Technol. 2013;6:648–70.View ArticleGoogle Scholar
- Newkirk DR, Sheppard AJ, Hubbard WD. Comparison of total fat, fatty acids, cholesterol, and other sterols in mayonnaise and imitation mayonnaise. J Am Oil Chem Soc. 1978;55:A548–9.View ArticleGoogle Scholar
- Karupaiah T, Noor MI, Sundram K. Dietary fatty acids and their influence on blood lipids and lipoproteins. In: Akoh CC, Lai O-M, editors. “Healthful Lipids” Champaign. Illinois: AOCS Press; 2005. p. 171–203.Google Scholar
- Amini M, Esmaillzadeh A, Shafaeizadeh S, Behrooz J, Zare M. Relationship between major dietary patterns and metabolic syndrome among individuals with impaired glucose tolerance. Nutrition. 2010;26:986–92.View ArticlePubMedGoogle Scholar
- Akter S, Nanri A, Pham NM, Kurotani K, Mizoue T. Dietary patterns and metabolic syndrome in a Japanese working population. Nutr & Metab. 2013;10:30.View ArticleGoogle Scholar
- Hanson GK. Inflammation, atherosclerosis and coronary artery disease. New Eng J Med. 2005;352:1658–95.Google Scholar
- Esposito K, Giugliano D. Diet and inflammation: a link to metabolic and cardiovascular diseases. Eur Heart J. 2006;27:15–20.View ArticlePubMedGoogle Scholar
- Mozaffarian D, Appel LJ, Van Horn L. Components of a cardioprotective diet- new insights. Circulation. 2011;123:2870–91.View ArticlePubMedGoogle Scholar
- Karupaiah T, Sundram K. Modulation of human postprandial lipemia by changing ratios of polyunsaturated to saturated (P/S) content of blended dietary fats: a cross-over design with repeated measures. Nutrition J. 2013;12:122.View ArticleGoogle Scholar
- Sundram K, Karupaiah T, Hayes C. Stearic acid-rich interesterified fat and trans-rich fat raise the LDL/HDL ratio and plasma glucose relative to palm olein in humans. Nutr & Metab. 2007;4:3.View ArticleGoogle Scholar
- Karupaiah T, Tan CH, Chinna K, Sundram K. The chain length of dietary saturated fatty acids affects human postprandial lipemia. J Am Coll Nutr. 2011;30:511–21.View ArticlePubMedGoogle Scholar
- DataBank F. Nutritionist Pro™-Nutritional analysis software. Stafford: Axxya Systems Licensee-, Hearst Corporation; 2005.Google Scholar
- Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem. 1974;20:470–5.Google Scholar
- Nägele U, Hagele EO, Sauer G, Wiedemann E, Lehmann P, Wahlefeld AW, Gruber W. Reagent for the enzymatic determination of serum total triglycerides with improved lipolytic efficiency. J Clin Chem Clin Biochem. 1984;22:165–74.PubMedGoogle Scholar
- Warnick GH, Benderson J, Albers JJ. Dextran sulfate- Mg2+ precipitation procedure for quantitation of high-density lipoprotein cholesterol. Clin Chem. 1982;28:1379–88.PubMedGoogle Scholar
- Friedewald WT, Levy RI, Frederickson D. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18:499–502.PubMedGoogle Scholar
- Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem. 1969;6:24.View ArticleGoogle Scholar
- Shimizu S, Yasui K, Tani Y, Yamada H. Acyl-CoA oxidase from candida troticalis. Biochem Biophys Res Commun. 1979;91:108–13.View ArticlePubMedGoogle Scholar
- Walldius G, Jungner I. Apolipoprotein B and A-1: risk indicators of coronary heart disease and targets for lipid-modifying therapy. J Intern Med. 2004;255:188–255.View ArticlePubMedGoogle Scholar
- Yagi K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods in Molecular Biol. 1998;108:101–6.Google Scholar
- Danesh J, Wheeler JG, Hirschfield GM, Eda S, Eiriksdottir G, Rumley A, et al. C-reactive protein and other circulating markers of inflammation in the prediction of coronary heart disease. New Eng J Med. 2004;350:1387–97.View ArticleGoogle Scholar
- Otvos JD. Measurement of lipoprotein subclass profiles by nuclear magnetic resonance spectroscopy. Clin Lab. 2002;48:171–80.PubMedGoogle Scholar
- Mattson FH, Grundy SM. Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man. J Lipid Res. 1985;26:194–202.PubMedGoogle Scholar
- Baum SJ, Kris-Etherton PM, Willett WC, Lichtenstein AH, Rudel LL, Maki KC, et al. Fatty acids in cardiovascular health and disease: a comprehensive update. J Clin Lipidol. 2012;6:216–34.View ArticlePubMedGoogle Scholar
- Kris-Etherton PM, Fleming JA. Emerging nutrition science on fatty acids and cardiovascular disease: nutritionists’ perspectives. Advan in Nutr. 2015;6:326S–37.View ArticleGoogle Scholar
- Medical Research Council. Controlled trial of soya-bean oil in myocardial infarction. Lancet. 1968;2:693–9.Google Scholar
- Miettinen M, Huttunen JK, Naukkarinen V, Strandberg T, Mattila S, Kumlin T, Sarna S. Multifactorial primary prevention of cardiovascular diseases in middle aged men. Risk factor changes, incidence, and mortality. JAMA. 1985;254:2097–102.View ArticlePubMedGoogle Scholar
- Miettinen M, Turpeinen O, Karvonen MJ, Pekkarinen M, Paavilainen E, Elosuo R. Dietary prevention of coronary heart disease in women: the Finnish mental hospital study. Int J Epidmiol. 1983;12:17–25.View ArticleGoogle Scholar
- Watts GF, Jackson P, Mandalia S, Brunt JN, Lewis ES, Coltart DJ, Lewis B. Nutrient intake and progression of coronary artery disease. Am J Cardiol. 1994;73:328–32.View ArticlePubMedGoogle Scholar
- Ma J, Folsom AR, Lewis L, Eckfeldt JH. Relation of plasma phospholipid and cholesterol ester fatty acid composition to carotid artery intima-media thickness: the Atherosclerosis Risk in Communities (ARIC) Study. Am J Clin Nutr. 1997;65:551–9.PubMedGoogle Scholar
- Ng TKW, Hayes KC, DeWitt GF, Jegathesan M, Satgunasingam N, Ong A, et al. Dietary palmitic and oleic acids exert similar effects on serum cholesterol and lipoprotein profiles in normocholesterolemic men and women. J Am Coll Nutr. 1992;11:383–90.View ArticlePubMedGoogle Scholar
- Choudhury N, Tan L, Truswell AS. Comparison of palm olein and olive oil: effects on plasma lipids and vitamin E in young adults. Am J Clin Nutr. 1995;61:1043–51.PubMedGoogle Scholar
- Young SG. Recent progress in understanding apolipoprotein B. Circulation. 1990;82:1574–94.View ArticlePubMedGoogle Scholar
- Berneis KK, Krauss RM. Metabolic origins and clinical significance of LDL heterogeneity. J Lipid Res. 2002;43:1363–79.View ArticlePubMedGoogle Scholar
- Cromwell WC, Otvos JD. Low-density lipoprotein particle number and risk for cardiovascular disease. Curr Atheroscler Rep. 2004;6:381–7.View ArticlePubMedGoogle Scholar
- Knopp RH, Retzlaff BM, Walden CE, Dowdy AA, Tsunehara CH, Austin MA, Nguyen T. A double-blind, randomized, controlled trial of the effects of two eggs per day in moderately hypercholesterolemic and combined hyperlipidemic subjects taught the NCEP step I diet. J Am Coll Nutr. 1997;16:551–61.PubMedGoogle Scholar
- Herron KL, Lofgren IE, Sharman M, Volek JS, Fernandez ML. High intake of cholesterol results in less atherogenic low-density lipoprotein particles in men and women independent of response classification. Metabolism. 2004;53:823–30.View ArticlePubMedGoogle Scholar
- Fritsche KL. The science of fatty acids and inflammation. Advan in Nutr. 2015;6:293S–301.View ArticleGoogle Scholar
- Rett BS, Whelan J. Increasing dietary linoleic acid does not increase tissue arachidonic acid content in adults consuming Western-type diets: a systematic review. Nutr & Metab. 2011;8:36.View ArticleGoogle Scholar
- Baer DJ, Judd JT, Clevidence BA, Tracy RP. Dietary fatty acids affect plasma markers of inflammation in healthy men fed controlled diets: a randomized crossover study. Am J Clin Nutr. 2004;79:969–73.PubMedGoogle Scholar
- Donovan SM, Shamir R. Introduction to the yoghurt in nutrition initiative and the first global summit on the health effects of yoghurt. Am J Clin Nutr. 2014;99:1209–11.View ArticleGoogle Scholar
- Cusack LK, Fernandez ML, Volek JS. The food matrix and sterol characteristics affect the plasma cholesterol lowering of phytosterol/phytostanol. Advan in Nutr. 2013;4:633–43.View ArticleGoogle Scholar
- Saito S, Takeshita M, Tomonobu K, Kudo N, Shiiba D, Hase T, et al. Dose-dependent cholesterol-lowering effect of a mayonnaise-type product with a main component of diacylglycerol-containing plant sterol esters. Nutrition. 2006;22:174–8.View ArticlePubMedGoogle Scholar