- Open Access
Long-term treatment with the pan-PPAR agonist tetradecylthioacetic acid or fish oil is associated with increased cardiac content of n-3 fatty acids in rat
Lipids in Health and Disease volume 11, Article number: 82 (2012)
Excess peroxisome proliferator-activated receptor (PPAR) stimulation has been associated with detrimental health effects including impaired myocardial function. Recently, supplementation with n-3 polyunsaturated fatty acids (PUFA) has been associated with improved left ventricular function and functional capacity in patients with dilated cardiomyopathy. We investigated the long-term effects of the pan-PPAR agonist tetradecylthioacetic acid (TTA) and/or high-dose fish oil (FO) on cardiac fatty acid (FA) composition and lipid metabolism. Male Wistar rats were given one out of four different 25% (w/v) fat diets: control diet; TTA diet; FO diet; or diet containing both TTA and FO.
After 50 weeks n-3 PUFA levels were increased by TTA and FO in the heart, whereas liver levels were reduced following TTA administration. TTA was associated with a decrease in arachidonic acid, increased activities of carnitine palmitoyltransferase II, fatty acyl-CoA oxidase, glycerol-3-phosphate acyltransferase, and fatty acid synthase in the heart. Furthermore, cardiac Ucp3 and Cact mRNA was upregulated.
Long-term treatment with the pan-PPAR agonist TTA or high-dose FO induced marked changes in PUFA composition and enzymatic activity involved in FA metabolism in the heart, different from liver. Changes included increased FA oxidation and a selective increase in cardiac n-3 PUFA.
Peroxisome proliferator-activated receptors (PPAR) are members of the nuclear hormone receptor family consisting of three subtypes (α, δ, and γ) with distinct and overlapping expression patterns . PPARα seems to be the primary transcription regulator of enzymes involved in fatty acid (FA) oxidation in the heart. A substrate switch from FA to glucose is thought to be a result of decreased PPARα activity . The healthy heart generates most of its energy as ATP through FA catabolism, which primarily occurs in mitochondria, but also to a small extent in peroxisomes . Thus, a continuous FA supply to the heart is important to sustain the contractile activity as this tissue can store and synthesize FA only to a limited extent . Excess PPARα stimulation may, however, have detrimental effects  including substrate overload of FA in the tissue, which is also associated with conditions like obesity and insulin resistance . Myocardial dysfunction could hereby be the result of an obesity-associated reduced glucose and increased FA utilization in heart [7, 8]. Lipotoxicity affects mitochondrial function through altered protein phosphorylation and increased levels of cytotoxic intermediate products of β-oxidation, which could lead to mitophagy (controlled degradation of mitochondria) or apoptosis .
A diet rich in n-3 polyunsaturated FA (PUFA) like eicosapentaenoic acid (EPA, C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3), has been associated with reduced triacylglycerol (TAG) levels, anti-inflammatory effects, and a lowered risk of cardiovascular disease and mortality [10, 11]. These beneficial effects may be less evident in patients with stable angina [12, 13], but particularly pronounced in patients with reduced ventricular function and heart failure . Very high doses of n-3 PUFA could, however, be pro-inflammatory and pro-oxidative .
Fibrates are a group of specific PPARα-targeted drugs being utilized during the past three decades due to their TAG-reducing effects. Their clinical gain, however, still remains to be demonstrated [16, 17], and their routine use in combined hyperlipidemia has recently been abandoned . Thus, excess PPARα activation may be associated with unfavorable metabolic effects that could counteract the apparent beneficial effects on lipid metabolism and inflammation.
Similarly to PUFA and fibrates, tetradecylthioacetic acid (TTA) has an especially pronounced affinity to PPARα . TTA is a saturated FA analogue with 16 carbon atoms and one sulphur atom at position three, belonging to a group of sulphur-substituted FA (3-thia FA) with pan-PPAR activation properties . It is known to reduce plasma TAG, probably due to hepatic proliferation of mitochondria and an increased β-oxidation of FA through PPAR-dependent mechanisms .
To this date inconsistent results from only a limited number of previous studies on the effects of PPARα agonists on cardiac substrate metabolism exist [22–27]. We hypothesized that treatment with the pan-PPAR agonist TTA and/or high-dose FO, would influence cardiac lipid metabolism and thereby affect FA composition. Data from liver was also applied to reveal organ specific changes in the heart.
Animal body weight and cardiac lipids
Mean (±SD) weight of the animals at study start was 266 (±32) g, and there were no significant weight differences between groups. During the study animals receiving TTA or the combination of TTA and FO had significantly reduced body weight gains compared to control (p < 0.001 for both groups). In addition, all groups had an average comparable feed intake . To eliminate the possibility of an effect-modification caused by the jejuno-gastric reflux surgical procedure, changes in body weight and plasma lipids were studied in an 11 week pilot study. This study did not reveal any statistically significant differences in neither body weight nor plasma lipids when comparing operated animals to controls.
After 50 weeks of dietary intervention there was no change in cardiac TAG as a result of TTA or FO treatment. There was, however, a significant increase in total cholesterol after TTA (mean ± SD, 3.21 ± 0.20 vs. 2.93 ± 0.24 μmol/g heart tissue, p < 0.001), and in phospholipids after FO (14.68 ± 0.91 vs. 13.69 ± 0.88 μmol/g heart tissue, p < 0.001) treatment.
Changes in cardiac fatty acids following TTA and/or FO administration
Two-way ANOVA indicated a significant increase in total FA (p = 0.002) in the heart of animals receiving TTA (Table 1). Total saturated FA (SFA) were decreased following TTA treatment (p < 0.001; Figure 1), while monounsaturated FA (MUFA) were decreased following FO treatment (p = 0.001; Table 1). The interaction (TTA*FO) for SFA was borderline significant in heart (p = 0.01). The level of mead acid (MA, C20:3n-9) was elevated following TTA treatment (p < 0.001) and decreased in animals receiving FO (p < 0.001; Table 1).
Total cardiac n-6 PUFA were decreased after both TTA and FO treatment (p < 0.001 for both; Figure 2a). The decrease in total n-6 PUFA after TTA treatment seemed to be due to a decrease in arachidonic acid (ARA) levels (p < 0.001; Figure 2b), while most other measured n-6 PUFA were increased (Additional file 1). The interaction (TTA*FO) was significant for total n-6 PUFA (p = 0.003) and borderline significant for ARA (p = 0.02).
There was a concomitant enrichment of total n-3 PUFA after TTA and FO treatment (p < 0.001 for both; Figure 3a). The total increase in n-3 PUFA after TTA administration was caused by significantly increased levels of EPA (Figure 3b), docosapentaenoic acid (n-3) (DPAn-3) (Figure 3c), and DHA (Figure 3d) (p < 0.001 for all), where DPAn-3 accounted for the foremost increase. After FO treatment, on the other hand, DPAn-3 levels decreased (p < 0.001), while EPA and DHA increased (<0.001 for both). The interaction (TTA*FO) was significant for total n-3 PUFA, EPA, DPAn-3, and DHA in heart (p < 0.001 for all). Total PUFA composition is provided as a supplementary table (Additional file 1).
As expected, the n-3/n-6 PUFA ratio was greatly increased following FO supplementation (p < 0.001). However, this ratio was also significantly increased after TTA treatment (p < 0.001; Table 1).
Desaturase activities were calculated as ratios of FA, which represent indirect activity indexes. TTA treatment led to a marked decrease in n-6 (p < 0.001) and an increase in n-3 (p < 0.001) Δ5 desaturase indexes. There was a concomitant increase in n-6 (p < 0.001) and decrease in n-3 (p < 0.001) Δ6 desaturase indexes. Furthermore, the anti-inflammatory index was increased for both supplements (p < 0.001; Table 1).
Changes in liver fatty acids following TTA and/or FO administration
As the liver is of crucial importance for FA metabolism, liver composition was also included for comparison (Table 2). There was a significant increase in C16:0 and a decrease in C18:0 levels in liver after dietary intervention with TTA (p < 0.001 for both), while FO decreased total SFA (p < 0.001). Total MUFA were increased after TTA and decreased following FO treatment (p < 0.001 for both). As in heart muscle, liver MA was elevated following TTA treatment and decreased in animals receiving FO (p < 0.001 for both).
Total liver n-6 PUFA were decreased after TTA and FO administration (p < 0.001, Figure 2a). Furthermore, n-3 PUFA were increased after FO (p < 0.001) but decreased after TTA (p < 0.001) treatment (Figure 3a). This decrease in n-3 PUFA in liver after TTA treatment was the opposite of what was seen in heart muscle (Figure 3a). The interaction (TTA*FO) was significant for n-6 PUFA (p = 0.004), n-3 PUFA (p = 0.004), EPA (p < 0.001), and DPAn-3 (p = 0.001).
Main changes in estimated desaturase indexes in liver were similar to changes in heart, except from an increase in Δ9 desaturase activity (C18 ratio) after TTA (p < 0.001) administration (Table 2). The hepatic anti-inflammatory index remained unchanged after TTA treatment.
Changes in the activities of key metabolic enzymes
TTA treatment was associated with reduced activity of carnitine palmitoyltransferase (CPT)-I in the heart (p < 0.001; Table 3), but the effect was less pronounced in the presence of malonyl-CoA (the natural inhibitor of CPT-I) (p < 0.001). The activity of CPT-II was increased after TTA treatment (p < 0.001; Table 3). FO treatment did not affect the activities of CPT-I or CPT-II.
Enzyme activities of fatty acyl-CoA oxidase (ACOX) and glycerol-3-phosphate acyltransferase (GPAT) in heart tissue were induced by TTA and FO (p < 0.001 for all), while fatty acid synthase (FAS) was induced by TTA (p < 0.001).
PPAR-targeted genes and cardiac expression at the mRNA level
A total of 16 different PPAR-targeted genes were selected and gene expression was measured with real-time PCR (qPCR) on total RNA isolated from heart tissue. Cpt-Ib (isoform of Cpt-I expressed in muscle) was one out of three genes that showed enhanced expression at the mRNA level after TTA administration (p = 0.003; Table 4). It was also induced by FO (p = 0.003). Also Cact mRNA, encoding the protein carnitine-acylcarnitine translocase, was upregulated after TTA treatment (p = 0.002). The Ucp3 gene (encoding the uncoupling protein 3) was upregulated (p < 0.001), while Ucp2 (p = 0.008), Pparδ (p = 0.002), and Pparγ (p < 0.001) were decreased after TTA administration. Fatp1 (encoding FA transport protein 1, involved in myocardial FA uptake) showed increased expression after FO supplementation (p = 0.001). There was no change in mRNA levels of PPARα following treatment with any supplement.
Long-term treatment with the pan-PPAR agonist TTA or high-dose FO induced marked changes in PUFA composition in heart muscle, resulting in decreased n-6 PUFA and increased n-3 PUFA. The significantly increased levels of EPA, DPAn-3, and DHA in the heart following TTA treatment was the opposite of what has previously been seen in plasma  and as shown in liver. The activity of enzymes involved in FA metabolism were also changed in the heart after TTA treatment, including increased CPT-II, ACOX, GPAT, and FAS activities as well as a significant upregulation of Ucp3 and Cact at the mRNA level.
There was no change in expression of Cd36 Fabp3, or Fatp1 mRNA, nor a change in TAG concentration, which gives no indication of enhanced entry of FA into the heart. Moreover, as TTA is reported to decrease the n-3 PUFA content in very low density lipoprotein (VLDL) particles , a selective increased secretion of n-3 PUFA from liver to plasma is unlikely. Dietary n-3 PUFA has been shown to decrease ARA in rat cardiac mitochondrial phospholipids , which is in accordance with the present study. While dietary FO decreased almost all n-6 PUFA in heart, TTA led to a specific decrease in ARA and at the same time an increase in most other n-6 PUFA. Both TTA and FO induced an increase in cardiac EPA and DHA. However, the increase in total n-3 PUFA as a TTA effect was particularly evident for DPAn-3, while cardiac DPAn-3 was decreased after FO treatment. Based on a previous study in platelets, ARA was shunted to the lipoxygenase pathway in the presence of DPAn-3 . Thus, an apparent link exists between ARA and DPAn-3, which might explain part of the mechanisms which underlie decreased ARA and increased DPAn-3 as exerted by TTA. These TTA effects were specific for heart muscle. Similarly, the PPARα-agonist clofibrate has previously been demonstrated to have profound effects on myocardial FA composition, including reduced ARA and increased DHA .
TTA induces proliferation of mitochondria and peroxisomes in the liver . Although there were no changes in the gene expression of Pgc1α Tfam or Nrf1 in heart after TTA administration, a significant increase in activity of CPT-II and gene expression of Cpt-Ib and Cact was observed. Furthermore, the peroxisomal β-oxidation system seemed to be induced as the ACOX activity was significantly increased both after TTA and FO administration. These findings suggest that TTA induces both mitochondrial and peroxisomal β-oxidation in heart, supported by two previous studies demonstrating increased myocardial FA oxidation by TTA treatment, associated with reduced cardiac efficiency [22, 27]. It has also been shown that TTA induces both CPT-II and ACOX in liver, and to an even larger extent compared to what we report in heart . TTA treatment also resulted in increased activities of cardiac GPAT and FAS. There was also increased enzyme activity of GPAT after FO treatment. This enzyme catalyzes the synthesis of lysophosphatidic acid from glycerol-3-phosphate and long-chain acyl-CoA . Thus, the portion of activated acyl-CoA that is not used for mitochondrial β-oxidation will be esterified into TAG and other glycerolipids, including phospholipids. The increased lipogenesis and TAG biosynthesis together with stimulated FA catabolism can thus explain the enrichment of n-3 PUFA in the heart both after TTA and FO treatment. n-3 PUFA, which are poorly oxidizable FA substrates compared to SFA , will most likely be diverted towards phospholipid synthesis and not β-oxidation. This could render n-3 PUFA less available for further metabolism. Altogether, an increased incorporation of n-3 PUFA into phospholipids in heart and a probably increased metabolism of n-3 PUFA in liver due to an extensive increase in CPT-II and ACOX  could explain why n-3 PUFA levels increase in heart and decrease in liver and plasma following TTA treatment. Together with the previously mentioned unfavorable metabolic effects of excess PPAR activation [5, 22], we consider the possibility that the observed increase of cardiac n-3 PUFA in the current study might not be solely beneficial.
We demonstrated a decrease in CPT-I, but at the same time an increased CPT-II activity in heart, which is in accordance with earlier results on TTA administration in liver . Although CPT-I has been assumed to be rate-limiting for FA oxidation in all tissues, a study has shown that about 50% of CPT-I activity can be inhibited before affecting the actual flux of β-oxidation in the heart . In addition, the present study showed that TTA affected CPT-I activity in the presence of malonyl-CoA by decreasing the sensitivity against this natural inhibitor of CPT-I . However, the reduced CPT-I activity could contribute to an inadequate transport or oxidation of long-chain FA in heart, while shorter FA that do not depend on CPT-I are completely oxidized in the mitochondria.
Expression of uncoupling protein Ucp3 mRNA is upregulated by PPAR activation, which is consistent with our current findings in the heart, with a strong significant increase after TTA and borderline significant increase after FO administration. This may lead to an increased FA transport and β-oxidation. Although its exact function remains largely unknown, UCP3 could act as an antioxidant and increased Ucp3 mRNA expression might be a response to an increase in oxidative stress . A previous study associated increased cardiac levels of UCP3 to decreased efficiency in rat heart after high fat feeding, most likely due to enhanced mitochondrial uncoupling .
The marked changes on FA metabolism induced by TTA could possibly result in essential FA deficiency due to the decreased levels of n-3 PUFA in liver and plasma . In support of this, TTA treatment was associated with elevated levels of MA both in heart and liver . A metabolic switch inducing the conversion of MA occurs as a result of low dietary levels of linoleic acid (LA) and α-linolenic acid (ALA). Such signs of essential FA deficiency have been reported in animals on a partially hydrogenated FO diet  or after treatment with fenofibrate , and may be caused by excess PPAR activation. Even though there was no change in PPARα mRNA after treatment with TTA or FO, we assume that the effects were primarily due to activation of PPARα. Increased expression of PPARα target genes following TTA  and FO  treatment have previously been shown in rodent models.
A beneficial effect of n-3 PUFA supplementation has recently been demonstrated in patients with reduced ventricular function and heart failure . Could the observed TTA effects on n-3 PUFA distribution in liver and heart tell us why n-3 PUFA supplements give favorable effects under certain conditions? There might be a link from the n-3 PUFA levels in heart to the association between TTA treatment and reduced cardiac efficiency in normal but not in diabetic mice [22, 27]. Furthermore, FO shows some similar effects to TTA on cardiac metabolism, perhaps through PPARα activating mechanisms, which might indicate detrimental effects by giving high doses. However, PPAR independent effects of TTA cannot be excluded. Whether the observed increase of n-3 PUFA in the hearts of rats treated with TTA and FO might also be related to PPAR effects in humans remain to be shown. Additionally, the effect of TTA on PUFA composition in the heart was directly associated with its TTA concentration (data not shown). It is also notable that PPARα and its agonists hold important properties beyond FA and glucose metabolism, like effects on amino acid metabolism . These aspects open for more extensive investigations of PPAR stimulation and cardiac metabolism.
In summary, long-term administration of the pan-PPAR agonist TTA or high-dose FO to rats, were associated with distinct effects on lipid metabolism in the heart and systemically. FA composition was changed, including a selective increase in n-3 PUFA in rat heart muscle, indicating a limited capacity of heart to metabolize the poorly oxidizable n-3 PUFA compared to SFA. Further, cardiac mitochondrial and peroxisomal FA oxidation seemed to be increased, together with a possible increased mitochondrial uncoupling after TTA treatment. FO in high doses could give similar effects to TTA in heart. These findings might altogether indicate a reduction in cardiac efficiency and are relevant for further studies on the effects of excess PPAR activation in relation to myocardial dysfunction development.
The animal experiments were standardized according to the Guidelines for the Care and Use of Experimental Animals, and the protocol was approved by the Norwegian State Board for Biological Experiments with Living Animals.
Male Wistar rats, aged eight to ten weeks, were obtained from Taconic Europe A/S (previously Möllegaard and Blomholtgaard, Ry, Denmark). They were housed in groups of five and maintained at a constant 12 h light–dark cycle at a temperature of 22 ± 1°C and a relative humidity of 55 ± 5%. Animals were acclimatized under these conditions for one week prior to study start and had free access to standard chow during the acclimation period and water at all times. During the feeding period the animals received one out of the following four diets for a period of 50 weeks: control diet with 25% (w/v) fat (23% w/v lard, 2% w/v soybean oil); diet supplemented with TTA (0.375% w/v TTA, 22.6 w/v lard, 2% w/v soybean oil); diet supplemented with FO (10.4% w/v EPAX 4020 TG, 12.6% w/v lard, 2% w/v soybean oil); or diet supplemented with TTA and FO (0.375% w/v TTA, 10.4% w/v EPAX 4020 TG, 12.2 w/v lard, 2% w/v soybean oil). These were complete diets containing 20% (w/v) protein from casein from bovine milk (Tine, Tolga, Norway). Lard (Ten Kate Vetten BV, Musselkanaal, Netherlands) and soybean oil (Dyets Inc., Bethlehem, PA, USA) were used as fat sources. Other ingredients were cornstarch, sucrose, fiber, AIN-93 G mineral mix, AIN-93 vitamin mix, L-cysteine, choline bitartrate (Dyets Inc.), and tert-butyl-hydroquinone (Sigma-Aldrich). FO was provided by EPAX AS. TTA was synthesized as previously described .
These animals were part of a larger study , where they all underwent a jejuno-gastric reflux surgical procedure. A separate experiment enduring 11 weeks were done comparing animals with and without operation, to make sure that the procedure did not affect the nutritional state in the animals. There was no difference in body weight or plasma lipids between the two groups (data not shown), and thus it can be assumed that the operation had no adverse effects regarding nutrition.
The animals were sacrificed with isoflurane (Forene, Abbott Laboratories, Abbott Park, IL) under non-fasting conditions. The abdomen was opened in the midline and blood was drawn by cardiac puncture and collected in BD Vacutainer tubes containing EDTA (Becton, Dickinson, and Company, Plymouth, UK). The heart and liver tissues were collected and immediately freeze-clamped as drainage of blood from the animal was complete. Plasma and tissue samples were stored at −80°C until analyses.
Quantification of lipids and fatty acids
Tissue samples were homogenized and lipids extracted with chloroform-methanol . Samples were evaporated under nitrogen and redissolved in isopropanol before analysis. Lipids were measured on the Hitachi 917 system (Roche Diagnostics, GmbH, Mannheim, Germany). Total cholesterol (CHOD-PAP) and TAG (GPO-PAP) kits were from Roche Diagnostics and the phospholipids kit from DiaSys Diagnostic Systems GmbH (Holzheim, Germany). FA methyl esters (FAME) were obtained by heating of lipids with methanol at 90°C for one hour. Sulphuric acid was used as a catalyst . After extraction into an organic solvent, FAME were analyzed by gas–liquid chromatography (GC). The gas chromatograph (GC 8000 TOP, Finnigan, USA) was equipped with a programmed temperature vaporization injector, flame-ionization detector, AS 800 autosampler, and with a fused silica capillary column DB1-ms (J & W Scientific, USA). Hydrogen was used as a carrier gas. Column temperature was programmed from 110 to 310°C with a gradient of 2.5°C/min. GC signal was acquired and evaluated with Chromeleon software (Dionex Corporation, USA). Peaks were identified by means of known FA standards and by means of mass spectra, obtained by GC/MS analysis (GCQ, Finnigan, USA) on the same column. Internal standard (C21:0) was used for quantification after calibration with known mixtures of FA standards. FA composition was presented as percentage by weight (wt%). The anti-inflammatory index was calculated using the formula: ((C22:6n-3 + C22:5n-3 + C20:3n-6 + C20:5n-3)/C20:4n-6)*100 .
Heart tissue samples were homogenized and fractionated as previously described . The activities of CPT-I and -II , ACOX [51, 52], GPAT , and FAS  were measured in the post-nuclear extracts.
Gene expression analyses
Total cellular RNA was purified from heart tissue using the RNeasy kit and the protocol for fibrous tissue (Qiagen GmbH, Hilden, Germany). RNA quantity was determined spectrophotometrically (NanoDrop 1000, NanoDrop products, Wilmington, DE, USA), while quality was evaluated by capillary electrophoresis (Agilent 2100 Bioanalyzer, Agilent Technologies Inc., Santa Clara, CA, USA) prior to gene analyses. RNA was reversely transcribed to cDNA in 100 μl reactions using TaqMan® Reverse Transcription Reagents (Applied Biosystems, Foster City, CA, USA). Samples were treated with RNase inhibitors as part of the protocol. Selected genes were analyzed using qPCR. The qPCR was performed with ABI PRISM 7900 HT Sequence Detection System (Applied Biosystems). The following 16 genes of interest were selected: Rn00566242 (Cpt-Ib), Rn00571166 (Ucp2), Rn00565874 (Ucp3), Rn00580241 (Pgc1α), Rn00580051_m1 (Tfam), Rn01455958_m1 (Nrf1), Rn00580728 (Cd36), Rn00588652 (Cact), Rn00577366 (Fabp3), Rn00563649 (Acadvl), Rn00566390 (Acadm), Rn00574634 (Acads), Rn00566193 (Pparα), Rn00565707 (Pparδ), Rn00440945 (Pparγ), and Rn00585821_m1 (Fatp1). All primer/probe sequences for the genes studied were obtained from Applied Biosystems. The MIQE guidelines for qPCR analyses were used when selecting house-keeping genes [55, 56]. Out of the three house-keeping genes originally included in the analyses: Hs99999901_s1 (18 S, Eurogentec S.A., Seraing, Belgium), Rn99999916_s1 (Gapdh, Applied Biosystems), and Rn00821065_g1 (Arbp, Applied Biosystems), 18 S was found to be the best using geNorm . This endogenous house-keeping gene was used to normalize the expression value of each gene in all samples.
The results were presented as means with their standard deviations (SD) for a minimum of eight and a maximum of fifteen rats per group. Gene expression data was normalized against the control diet group. Data was evaluated by two-way ANOVA for treatment additivity and synergy . Results were not adjusted for multiple comparisons, and thus p-values <0.01 were considered significant. Statistics were performed by using PASW Statistics for Windows, version 18 (SPSS Inc., Chicago, IL, USA).
Fatty acyl-CoA oxidase
Analysis of variance
Cluster of differentiation 36
Docosapentaenoic acid (n-3)
Fatty acid binding protein
Fatty acid methyl ester
Fatty acid synthase
Fatty acid transport protein
Monounsaturated fatty acids
Peroxisome proliferator-activated receptor
Polyunsaturated fatty acids
Saturated fatty acids
Very low density lipoprotein1.
Madrazo JA, Kelly DP: The PPAR trio: Regulators of myocardial energy metabolism in health and disease. J Mol Cell Cardiol. 2008, 44: 968-975. 10.1016/j.yjmcc.2008.03.021
Neubauer S: The failing heart–an engine out of fuel. N Engl J Med. 2007, 356: 1140-1151. 10.1056/NEJMra063052
Grynberg A: Effectors of fatty acid oxidation reduction: promising new anti-ischaemic agents. Curr Pharm Des. 2005, 11: 489-509. 10.2174/1381612053382061
Stanley WC, Recchia FA, Lopaschuk GD: Myocardial substrate metabolism in the normal and failing heart. Physiol Rev. 2005, 85: 1093-1129. 10.1152/physrev.00006.2004
Sambandam N, Morabito D, Wagg C, Finck BN, Kelly DP, Lopaschuk GD: Chronic activation of PPARalpha is detrimental to cardiac recovery after ischemia. Am J Physiol Heart Circ Physiol. 2006, 290: H87-95.
Fujita M, Momose A, Ohtomo T, Nishinosono A, Tanonaka K, Toyoda H, Morikawa M, Yamada J: Upregulation of fatty acyl-CoA thioesterases in the heart and skeletal muscle of rats fed a high-fat diet. Biol Pharm Bull. 2011, 34: 87-91. 10.1248/bpb.34.87
Wright JJ, Kim J, Buchanan J, Boudina S, Sena S, Bakirtzi K, Ilkun O, Theobald HA, Cooksey RC, Kandror KV, Abel ED: Mechanisms for increased myocardial fatty acid utilization following short-term high-fat feeding. Cardiovasc Res. 2009, 82: 351-360.
Finck BN, Lehman JJ, Leone TC, Welch MJ, Bennett MJ, Kovacs A, Han X, Gross RW, Kozak R, Lopaschuk GD, Kelly DP: The cardiac phenotype induced by PPARalpha overexpression mimics that caused by diabetes mellitus. J Clin Invest. 2002, 109: 121-130.
Graier WF, Malli R, Kostner GM: Mitochondrial protein phosphorylation: instigator or target of lipotoxicity?. Trends Endocrinol Metab. 2009, 20: 186-193. 10.1016/j.tem.2009.01.004
Harris WS, Miller M, Tighe AP, Davidson MH, Schaefer EJ: Omega-3 fatty acids and coronary heart disease risk: clinical and mechanistic perspectives. Atherosclerosis. 2008, 197: 12-24. 10.1016/j.atherosclerosis.2007.11.008
Moertl D, Hammer A, Steiner S, Hutuleac R, Vonbank K, Berger R: Dose-dependent effects of omega-3-polyunsaturated fatty acids on systolic left ventricular function, endothelial function, and markers of inflammation in chronic heart failure of nonischemic origin: a double-blind, placebo-controlled, 3-arm study. Am Heart J. 2011, 161: 915 e911-919.
Manger MS, Strand E, Ebbing M, Seifert R, Refsum H, Nordrehaug JE, Nilsen DW, Drevon CA, Tell GS, Bleie O: Dietary intake of n-3 long-chain polyunsaturated fatty acids and coronary events in Norwegian patients with coronary artery disease. Am J Clin Nutr. 2010, 92: 244-251. 10.3945/ajcn.2010.29175
Saravanan P, Davidson NC, Schmidt EB, Calder PC: Cardiovascular effects of marine omega-3 fatty acids. Lancet. 2010, 376: 540-550. 10.1016/S0140-6736(10)60445-X
Nodari S, Triggiani M, Campia U, Manerba A, Milesi G, Cesana BM, Gheorghiade M: Dei Cas L: Effects of n-3 polyunsaturated fatty acids on left ventricular function and functional capacity in patients with dilated cardiomyopathy. J Am Coll Cardiol. 2011, 57: 870-879. 10.1016/j.jacc.2010.11.017
Skulas-Ray AC, Kris-Etherton PM, Harris WS: Vanden Heuvel JP, Wagner PR, West SG: Dose–response effects of omega-3 fatty acids on triglycerides, inflammation, and endothelial function in healthy persons with moderate hypertriglyceridemia. Am J Clin Nutr. 2011, 93: 243-252. 10.3945/ajcn.110.003871
Jackevicius CA, Tu JV, Ross JS, Ko DT, Carreon D, Krumholz HM: Use of fibrates in the United States and Canada. JAMA. 2011, 305: 1217-1224. 10.1001/jama.2011.353
Saha SA, Arora RR: Hyperlipidaemia and cardiovascular disease: do fibrates have a role?. Curr Opin Lipidol. 2011, 22: 270-276. 10.1097/MOL.0b013e32834701c3
Goldfine AB, Kaul S, Hiatt WR: Fibrates in the treatment of dyslipidemias–time for a reassessment. N Engl J Med. 2011, 365: 481-484. 10.1056/NEJMp1106688
Westergaard M, Henningsen J, Svendsen ML, Johansen C, Jensen UB, Schroder HD, Kratchmarova I, Berge RK, Iversen L, Bolund L: Modulation of keratinocyte gene expression and differentiation by PPAR-selective ligands and tetradecylthioacetic acid. J Invest Dermatol. 2001, 116: 702-712. 10.1046/j.1523-1747.2001.01329.x
Wensaas AJ, Rustan AC, Rokling-Andersen MH, Caesar R, Jensen J, Kaalhus O, Graff BA, Gudbrandsen OA, Berge RK, Drevon CA: Dietary supplementation of tetradecylthioacetic acid increases feed intake but reduces body weight gain and adipose depot sizes in rats fed on high-fat diets. Diabetes Obes Metab. 2009, 11: 1034-1049. 10.1111/j.1463-1326.2009.01092.x
Dyroy E, Yndestad A, Ueland T, Halvorsen B, Damas JK, Aukrust P, Berge RK: Antiinflammatory effects of tetradecylthioacetic acid involve both peroxisome proliferator-activated receptor alpha-dependent and -independent pathways. Arterioscler Thromb Vasc Biol. 2005, 25: 1364-1369. 10.1161/01.ATV.0000171982.57713.96
Hafstad AD, Khalid AM, Hagve M, Lund T, Larsen TS, Severson DL, Clarke K, Berge RK, Aasum E: Cardiac peroxisome proliferator-activated receptor-alpha activation causes increased fatty acid oxidation, reducing efficiency and post-ischaemic functional loss. Cardiovasc Res. 2009, 83: 519-526. 10.1093/cvr/cvp132
Berge RK, Flatmark T, Osmundsen H: Enhancement of long-chain acyl-CoA hydrolase activity in peroxisomes and mitochondria of rat liver by peroxisomal proliferators. Eur J Biochem. 1984, 141: 637-644. 10.1111/j.1432-1033.1984.tb08239.x
Madsen L, Froyland L, Dyroy E, Helland K, Berge RK: Docosahexaenoic and eicosapentaenoic acids are differently metabolized in rat liver during mitochondria and peroxisome proliferation. J Lipid Res. 1998, 39: 583-593.
Wergedahl H, Liaset B, Gudbrandsen OA, Lied E, Espe M, Muna Z, Mork S, Berge RK: Fish protein hydrolysate reduces plasma total cholesterol, increases the proportion of HDL cholesterol, and lowers acyl-CoA:cholesterol acyltransferase activity in liver of Zucker rats. J Nutr. 2004, 134: 1320-1327.
Tian Q, Grzemski FA, Panagiotopoulos S, Ahokas JT: Peroxisome proliferator-activated receptor alpha agonist, clofibrate, has profound influence on myocardial fatty acid composition. Chemico-biological interactions. 2006, 160: 241-251. 10.1016/j.cbi.2006.02.003
Khalid AM, Hafstad AD, Larsen TS, Severson DL, Boardman N, Hagve M, Berge RK, Aasum E: Cardioprotective effect of the PPAR ligand tetradecylthioacetic acid in type 2 diabetic mice. Am J Physiol Heart Circ Physiol. 2011, 300: H2116-2122. 10.1152/ajpheart.00357.2010
Vigerust NF, Cacabelos D, Burri L, Berge K, Wergedahl H, Christensen B, Portero-Otin M, Viste A, Pamplona R, Berge RK, Bjorndal B: Fish oil and 3-thia fatty acid have additive effects on lipid metabolism but antagonistic effects on oxidative damage when fed to rats for 50 weeks. J Nutr Biochem. 2012,
Grav HJ, Tronstad KJ, Gudbrandsen OA, Berge K, Fladmark KE, Martinsen TC, Waldum H, Wergedahl H, Berge RK: Changed energy state and increased mitochondrial beta-oxidation rate in liver of rats associated with lowered proton electrochemical potential and stimulated uncoupling protein 2 (UCP-2) expression: evidence for peroxisome proliferator-activated receptor-alpha independent induction of UCP-2 expression. J Biol Chem. 2003, 278: 30525-30533. 10.1074/jbc.M303382200
Muna ZA, Gudbrandsen OA, Wergedahl H, Bohov P, Skorve J, Berge RK: Inhibition of rat lipoprotein oxidation after tetradecylthioacetic acid feeding. Biochem Pharmacol. 2002, 63: 1127-1135. 10.1016/S0006-2952(01)00934-0
O'Shea KM, Khairallah RJ, Sparagna GC, Xu W, Hecker PA, Robillard-Frayne I, Des Rosiers C, Kristian T, Murphy RC, Fiskum G, Stanley WC: Dietary omega-3 fatty acids alter cardiac mitochondrial phospholipid composition and delay Ca2+−induced permeability transition. J Mol Cell Cardiol. 2009, 47: 819-827. 10.1016/j.yjmcc.2009.08.014
Kaur G, Cameron-Smith D, Garg M, Sinclair AJ: Docosapentaenoic acid (22:5n-3): a review of its biological effects. Progress in lipid research. 2011, 50: 28-34. 10.1016/j.plipres.2010.07.004
Raspe E, Madsen L, Lefebvre AM, Leitersdorf I, Gelman L, Peinado-Onsurbe J, Dallongeville J, Fruchart JC, Berge R, Staels B: Modulation of rat liver apolipoprotein gene expression and serum lipid levels by tetradecylthioacetic acid (TTA) via PPARalpha activation. Journal of lipid research. 1999, 40: 2099-2110.
Wendel AA, Lewin TM, Coleman RA: Glycerol-3-phosphate acyltransferases: rate limiting enzymes of triacylglycerol biosynthesis. Biochim Biophys Acta. 2009, 1791: 501-506. 10.1016/j.bbalip.2008.10.010
Berge RK, Madsen L, Vaagenes H, Tronstad KJ, Gottlicher M, Rustan AC: In contrast with docosahexaenoic acid, eicosapentaenoic acid and hypolipidaemic derivatives decrease hepatic synthesis and secretion of triacylglycerol by decreased diacylglycerol acyltransferase activity and stimulation of fatty acid oxidation. Biochem J. 1999, 343 (Pt 1): 191-197.
Madsen L, Berge RK: 3-Thia fatty acid treatment, in contrast to eicosapentaenoic acid and starvation, induces gene expression of carnitine palmitoyltransferase-II in rat liver. Lipids. 1999, 34: 447-456. 10.1007/s11745-999-0384-6
Eaton S, Bartlett K, Quant PA: Carnitine palmitoyl transferase I and the control of beta-oxidation in heart mitochondria. Biochem Biophys Res Commun. 2001, 285: 537-539. 10.1006/bbrc.2001.5201
Dowell P, Hu Z, Lane MD: Monitoring energy balance: metabolites of fatty acid synthesis as hypothalamic sensors. Annu Rev Biochem. 2005, 74: 515-534. 10.1146/annurev.biochem.73.011303.074027
Azzu V, Jastroch M, Divakaruni AS, Brand MD: The regulation and turnover of mitochondrial uncoupling proteins. Biochim Biophys Acta. 1797, 785-791.
Cole MA, Murray AJ, Cochlin LE, Heather LC, McAleese S, Knight NS, Sutton E, Jamil AA, Parassol N, Clarke K: A high fat diet increases mitochondrial fatty acid oxidation and uncoupling to decrease efficiency in rat heart. Basic research in cardiology. 2011, 106: 447-457. 10.1007/s00395-011-0156-1
Le HD, Meisel JA, de Meijer VE, Gura KM, Puder M: The essentiality of arachidonic acid and docosahexaenoic acid. Prostaglandins Leukot Essent Fatty Acids. 2009, 81: 165-170. 10.1016/j.plefa.2009.05.020
Kryvi H, Christiansen E, Tangeras A, Flatmark T: Effects of essential fatty acid deficiency on mitochondria and peroxisomes in rat hepatocytes with special reference to a partially hydrogenated fish oil diet. Biochim Biophys Acta. 1986, 881: 87-92. 10.1016/0304-4165(86)90100-5
Oosterveer MH, Grefhorst A, van Dijk TH, Havinga R, Staels B, Kuipers F, Groen AK, Reijngoud DJ: Fenofibrate simultaneously induces hepatic fatty acid oxidation, synthesis, and elongation in mice. J Biol Chem. 2009, 284: 34036-34044. 10.1074/jbc.M109.051052
Zuniga J, Cancino M, Medina F, Varela P, Vargas R, Tapia G, Videla LA, Fernandez V: N-3 PUFA supplementation triggers PPAR-alpha activation and PPAR-alpha/NF-kappaB interaction: anti-inflammatory implications in liver ischemia-reperfusion injury. PLoS One. 2011, 6: e28502- 10.1371/journal.pone.0028502
Sheikh K, Camejo G, Lanne B, Halvarsson T, Landergren MR, Oakes ND: Beyond lipids, pharmacological PPARalpha activation has important effects on amino acid metabolism as studied in the rat. Am J Physiol Endocrinol Metab. 2007, 292: E1157-1165.
Spydevold O, Bremer J: Induction of peroxisomal beta-oxidation in 7800 C1 Morris hepatoma cells in steady state by fatty acids and fatty acid analogues. Biochim Biophys Acta. 1989, 1003: 72-79. 10.1016/0005-2760(89)90101-X
Christensen BJ, Berge K, Wergedahl H, Bohov P, Berge RK, Svendsen E, Viste A: Bioactive Fatty Acids Reduce Development of Gastric Cancer Following Duodenogastric Reflux in Rats. Surg Sci. 2012, 3: 34-42. 10.4236/ss.2012.31006.
Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959, 37: 911-917. 10.1139/o59-099
Kates M: General analytical procedures. Techniques of Lipidology. Edited by: Amsterdam MD. 1986, 112-185. Elsevier Science Publishers,
Chavali SR, Zhong WW, Utsunomiya T, Forse RA: Decreased production of interleukin-1-beta, prostaglandin-E2 and thromboxane-B2, and elevated levels of interleukin-6 and -10 are associated with increased survival during endotoxic shock in mice consuming diets enriched with sesame seed oil supplemented with Quil-A saponin. Int Arch Allergy Immunol. 1997, 114: 153-160. 10.1159/000237661
Berge RK, Flatmark T, Christiansen EN: Effect of a high-fat diet with partially hydrogenated fish oil on long-chain fatty acid metabolizing enzymes in subcellular fractions of rat liver. Arch Biochem Biophys. 1987, 252: 269-276. 10.1016/0003-9861(87)90031-2
Berge RK, Nilsson A, Husoy AM: Rapid stimulation of liver palmitoyl-CoA synthetase, carnitine palmitoyltransferase and glycerophosphate acyltransferase compared to peroxisomal beta-oxidation and palmitoyl-CoA hydrolase in rats fed high-fat diets. Biochim Biophys Acta. 1988, 960: 417-426. 10.1016/0005-2760(88)90050-1
Bates EJ, Saggerson D: A selective decrease in mitochondrial glycerol phosphate acyltransferase activity in livers from streptozotocin-diabetic rats. FEBS Lett. 1977, 84: 229-232. 10.1016/0014-5793(77)80694-7
Skorve J, al-Shurbaji A, Asiedu D, Bjorkhem I, Berglund L, Berge RK: On the mechanism of the hypolipidemic effect of sulfur-substituted hexadecanedioic acid (3-thiadicarboxylic acid) in normolipidemic rats. J Lipid Res. 1993, 34: 1177-1185.
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL: The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009, 55: 611-622. 10.1373/clinchem.2008.112797
Nolan T, Hands RE, Bustin SA: Quantification of mRNA using real-time RT-PCR. Nat Protoc. 2006, 1: 1559-1582. 10.1038/nprot.2006.236
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3: RESEARCH0034-
Slinker BK: The statistics of synergism. J Mol Cell Cardiol. 1998, 30: 723-731. 10.1006/jmcc.1998.0655
We are grateful to Liv Kristine Øysæd, Svein Krüger, Kari Williams, and Randi Sandvik for excellent technical assistance. We also thank the employees at the University of Bergen lab animal facility for their important contributions during the animal experiments.
This work was supported by grants from the Western Norway Regional Health Authority, grant no. 911219; the Research Council of Norway, grant no. 170489/V40; NordForsk, grant no. 070010, MitoHealth; and the European Community's Seventh Framework Programme (FP7/2007-2013), grant no. 201668, AtheroRemo.
The authors declare that they have no competing interests.
The authors’ responsibilities were as follows: ON, KB, HW, AV, and RKB: developed the study concept and design and supervised the study; ON, KB, HW, AV, and RKB: obtained funding; ES, CB, PB, BJC, KB, and HW: collected materials and data; ES, BB, ON, LB, PB, KB, HW, AV, and RKB: provided administrative, technical and material support; ES, BB, ON, LB, PB, and RKB: analyzed and interpreted data; ES: conducted the statistical analysis; ES: drafted the manuscript, including figures and tables; and ES, BB, ON, LB, CB, PB, BJC, KB, HW, AV, and RKB: contributed to the critical revision of the manuscript. All authors read and approved the final manuscript.
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Strand, E., Bjorndal, B., Nygard, O. et al. Long-term treatment with the pan-PPAR agonist tetradecylthioacetic acid or fish oil is associated with increased cardiac content of n-3 fatty acids in rat. Lipids Health Dis 11, 82 (2012) doi:10.1186/1476-511X-11-82
- Heart metabolism
- Peroxisome proliferator-activated receptor
- Polyunsaturated fatty acids
- Tetradecylthioacetic acid