Effects of thyroxine and 1-methyl, 2-mercaptoimidazol on phosphoinositides synthesis in rat liver
© Babenko and Krasilnikova; licensee BioMed Central Ltd. 2004
Received: 12 November 2004
Accepted: 10 December 2004
Published: 10 December 2004
Phosphoinositides mediate one of the intracellular signal transduction pathways and produce a class of second messengers that are involved in the action of hormones and neurotransmitters on target cells. Thyroid hormones are well known regulators of lipid metabolism and modulators of signal transduction in cells. However, little is known about phosphoinositides cycle regulation by thyroid hormones. The present paper deals with phosphoinositides synthesis de novo and acylation in liver at different thyroid status of rats.
The experiments were performed in either the rat liver or hepatocytes of 90- and 720-day-old rats. Myo-[3H]inositol, [14C]CH3COONa, [14C]oleic and [3H]arachidonic acids were used to investigate the phosphatidylinositol (PtdIns), phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate (PtdInsP2) synthesis. 1-methyl, 2-mercaptoimidazol-induced hypothyroidism was associated with the decrease of myo-[3H]inositol and [3H]arachidonic acids incorporation into liver phosphoinositides and total phospholipids, respectively. The thyroxine (L-T4) injection to hypothyroid animals increased the hormones contents in blood serum and PtdInsP2 synthesis de novo as well as [3H]arachidonic acids incorporation into the PtdIns and PtdInsP2. Under the hormone action, the [14C]oleic acid incorporation into PtdIns reduced in the liver of hypothyroid animals. A single injection of L-T4 to the euthyroid [14C]CH3COONa-pre-treated animals or addition of the hormone to a culture medium of hepatocytes was accompanied by the rapid prominent increase in the levels of the newly synthesized PtdIns and PtdInsP2 and in the mass of phosphatidic acid in the liver or the cells.
The data obtained have demonstrated that thyroid hormones are of vital importance in the regulation of arachidonate-containing phosphoinositides metabolism in the liver. The drug-induced malfunction of thyroid gland noticeably changed the phosphoinositides synthesis de novo. The L-T4 injection to the animals was followed by the time-dependent increase of polyphosphoinositide synthesis in the liver. The both long-term and short-term hormone effects on the newly synthesized PtdInsP2 have been determined.
The phosphoinositides is a family of lipids which members play an essential role in the receptor-mediated intracellular signaling cascades, vesicle trafficking and cytoskeletal rearrangements [for review see [1, 2]] and, therefore, are crucial for the adaptation and survival of cells. Because of their importance in numerous signaling events, the phosphoinositides require an absolutely tight temporal and spatial regulation of synthesis and degradation, enabling the cell to maintain organelle identity and housekeeping functions. A rapid agonist-dependent burst of phosphoinositides biosynthesis was the first feature of the polyphosphoinositide signaling pathway to be discovered, illustrating that synthesis is tightly coupled to degradation.
The two pools of phosphoinositides are supposed to exist in the cells [3, 4]. One of these is sensitive to the hormone-induced hydrolysis, and the other is hydrolysis-insensitive. The biosynthesis of phosphatidylinositol (PtdIns) is the two-component process composed of cytidyltransferase followed by a synthase [for review see [5, 6]]. The first detailed study of the occurrence and localization of the PtdIns synthase activity in various tissues from guinea pig was carried out by Benjamins and Agranoff . They detected the enzyme activity in all the tissues tested, including brain, liver, kidney, heart, lung and spleen. The PtdIns synthase activity was also detected in the endoplasmic reticulum, plasma membranes, Golgi apparatus and nuclei. The hormone-sensitive pool of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) in the plasma  and nuclear  membranes re-synthesized in these membrane fractions. The PtdIns synthesized de novo in the endoplasmic reticulum could be converted to the glycosylPtdIns or transferred by transfer proteins to other cellular compartments where the lipid was used for the phosphatidylinositol 4-phosphate (PtdInsP), PtdInsP2 and other polyphosphoinositides production [10, 11].
The regulation of the key enzymes of the PtdIns synthesis pathway: the CDP-diacylglycerol (DAG) synthase and PtdIns synthase were examined . Expression of the PtdIns synthase gene caused the overproduction of the both of the PtdIns synthase and PtdIns:inositol exchange reactions, indicating that the gene encode the both enzymes. However, the overexpression of PtdIns synthase or CDP-DAG synthase alone or in combination in the COS-7 cells did not enhance the rate of the PtdIns biosynthesis and did not result in a significant proportional increase in the CDP-DAG and PtdIns cellular levels. The CDP-DAG synthase activity was inhibited by polyphosphoinositides in vitro . This makes possible to suggest that these end products of the pathway may function as the feedback inhibitors of PtdIns biosynthesis in vivo. The PtdIns synthase activity has been shown to be upregulated after the hormone-induced phospholipase C (PLC) mediated hydrolysis of phosphatidylinositol-polyphosphates [14, 15]. The efficiency of the PtdIns synthesis is dependent on the CDP-DAG fatty acids composition [for review see [5, 6]]. In the bovine brain, the CDP-DAG has been shown to present predominantly as the 1-steroyl, 2-arachidonyl which are also the main PtdIns components.
Thyroid hormones are of vital importance in maintaining the initial level of phospholipids in cell membranes and fatty acids composition of the lipids. On the other hand, there is a little literature regarding the hormone regulation of phosphoinositides exchange. The levels of [32P]phosphoinositides and inositol 1,4,5-trisphosphate were found to be significantly lower in the hypothyroid rat hearts . The effect of hypothyroidism on the insulin- and epinephrine-stimulated phosphoinositide metabolism has been investigated in the rat adipocytes . The hypothyroidism enhanced the insuline-mediated phosphoinositides synthesis. The hypothyroidism caused a significant increase in both the basal and ouabain-stimulated accumulation of [3H]inositol phosphate in the hypothalamic slices, whereas the thyroxine (L-T4) completely restored the hypothalamic [3H]inositol phosphate formation . The results indicate that the negative feedback action of the thyroid hormone may occur at a post-receptor site in the hypothalamus. The thyroid hormones might participate in regulating the muscarinic cholinergic neurotransmission in the adult rats striatum via the stimulatory action on the inositol phosphate formation in the [3H]inositol pre-labeled tissue . Thus, it becomes evident that the thyroid gland malfunction leads to the prominent disturbances of signal transduction in the adipocytes and nervous cells via the changes of phosphoinositides metabolism.
In the paper, we examined the influence of the drug- and L-T4-altered thyroid status of animals on the phosphoinositides synthesis in the liver. Thus, it was determined that the hypothyroidism decreased considerably the levels of the newly synthesized lipids in the liver but thyroid hormones increased the synthesis of the polyphosphoinositides de novo and the arachidonic acid incorporation into the PtdIns and PtdInsP2. A single injection of the L-T4 to the euthyroid rats lead to the rapid and transient decrease of the newly synthesized PtdInsP2 followed by the increase of the PtdIns and PtdInsP2 levels in the liver.
Results and Discussion
The present paper considers the influence of thyroid functional status on the phosphoinositides synthesis in the liver. To determine the role of thyroid hormones in the regulation of phosphoinositides synthesis de novo and lipids fatty acid remodeling, the euthyroid, MMI (1-methyl, 2-mercaptoimidazol)- and MMI+L-T4-treated rats and intact animals after the single hormone injection has been studied.
It has been reported that the L-T4 induced hydrolysis of PtdInsP2 and inositol phosphates and DAG formation at the early stages of hormone action on the [14C]oleic and [14C]linoleic acid pre-labeled hepatocytes of adult 90-day-old rats [20, 21]. The L-T4-mediated PLC activation was accompanied by the protein kinase C (PKC) translocation to membranes  and PKC dependent stimulation of mitogen-activated protein kinase  and acylation of phospholipids and triacylglycerol synthesis . The PtdInsP2-specific PLC activation in the L-T4-stimulated cells was a short-lived event. The hormone-stimulated rise in inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) was followed by its conversion into the biologically inactive inositol 1,4 -bisphosphate and inositol 1-phosphate .
Thyroxine and triiodothyronine contents in the blood serum of the rats of different thyroid states and age.
128 ± 3.80
1.21 ± 0.20
29.5 ± 5.00**
0.83 ± 0.01*
57.3 ± 7.30***
1.61 ± 0.10***
654 ± 50.0**
5.78 ± 0.20**
675 ± 98.5**
15.9 ± 4.10**
70.7 ± 11.6
1.37 ± 0.07
730 ± 15**
5.8 ± 1.5**
537 ± 128**
4.9 ± 0.0**
In addition to its established role as a precursor for the signaling molecules (Ins(1,4,5)P3 and inositol 3,4,5-trisphosphate), the PtdInsP2 is now recognized as an important plasma membrane signal that activates the PLD [for review see ] and thus establishes the sites for vesicular trafficking, membrane movement and cytoskeletal assembly. It has been demonstrated in the mammalian cells that the PtdInsP2 is a membrane-associated cofactor of PLD [26, 27]. The rapid stimulation of the PtdInsP2-specific PLC by the L-T4 in the rat hepatocytes of adult 90-day-old rats was followed by the prominent PLD activation . The PLD response in the stimulated cells was reduced by the both PKC inhibitor and a high affinity ligand of PtdInsP2 – neomycin. Neomycin does not directly interact with PLC and PLD but interacts with the endogenous membrane PtdInsP2. Inclusion of the PtdInsP2 in mixed phosphatidylcholine/phosphatidylethanolamine liposomes  and stimulation of the phosphoinositide 5-kinase by an addition of the MgATP  greatly potentiates the PLD activation. Direct evidence that phosphoinositide kinase is involved in the PLD activation comes with the use of an inhibitory antibody for this enzyme . The results strongly demonstrate that the PLD activation requires the enhanced PtdInsP2 synthesis and the resting cellular levels of PtdInsP2 are insufficient for enzyme stimulation. The role of PKC has been determined in the regulation of PtdInsP2synthesis in the liver  and other cell types . The PKC translocation to membranes and enzyme activation are initial steps in the PLD [for review see ] and phosphoinositide 5-kinase  stimulation. The lack of PKC/PLD response  correlates with the suppressed ability of the L-T4 to stimulate the PtdInsP2 synthesis in the liver cells of the 720-day-old rats (Fig. 1B). However, in the liver cells of adult animals the L-T4 -induced PKC activation and elevation of PtdInsP2 synthesis might lead to the PLD activation.
Rapid effect of L-T4 on phosphatidic acid and phosphatidylinositol contents in the isolated hepatocytes.
9,29 ± 0,71
17,7 ± 2,94 *
13,6 ± 0,62
13,7 ± 1,83
It is well documented that the efficiency of polyphoshoinositide derived second messanger DAG in signaling pathways is closely dependent on the degree of its unsaturation. The unsaturated fatty acids are incorporated into the sn-2 position of the phospholipids by the deacylation-reacylation reactions. Some investigations demonstrated the changes of the fatty acid composition of membraneous lipids at the hypo- and hyperthyroid state of the rats [for review see ]. There were generally a reciprocal changes in membranes arachidonic acid contents, namely, a decrease in the hypothyroidism and its increase after the thyroid hormone injection. The L-T3 administration to the euthyroid rats increases the saturated fatty acids and arachidonate/linoleate ratio of PtdIns in the liver cell mitochondria .
The MMI did not change markedly the 14C/3H-labeling of PtdIns and PtdInsP2 in the liver slices (Figure 3B,3D). The L-T4 administration to the rats increased the incorporation of [3H]arachidonic acid into the PtdIns and PtdInsP2 in the hypothyroid liver (Table 3B) and decreased the content of the oleate-labeled PtdIns in the liver slices (Table 3D). The incorporation of the [14C]oleic acid into the PtdInsP2 did not differ between the liver slices of control, drug- and hormone-treated animals. As can be seen from the Table 1, the MMI reduced the T4 and T3 levels in the blood serum, but did not remove completely the hormones from organism. The T3 content in the serum of the drug-treated animals was relatively high as compared with control animals. However, the MMI reduced the T4 level by 77%. The T4 administration to the MMI-treated rats increased significantly T3 and T4 contents in the serum. It seems possible that the 14C/3H fatty acids incorporation into the phosphoinositides was rather regulated by the T3 than T4, although the both hormones are participated in the regulation of the other phospholipids acylation in the rat liver.
Materials and Methods
myo-[3H]-inositol (58 mCi/mmol), [14C]oleic acid (58 mCi/mmol) and [3H]arachidonic acid (60 Ci/mmol) – Amersham Corp. and [14C]CH3COONa (25 mCi/mmol) – BPO Isotop (Russia); silica gel from Woelm (Germany). Phosphatidylinositol, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate lipid standards were obtained from Sigma (USA). T4 and 1-methyl, 2-mercaptoimidazol were from Zdorov'e Trudyaschikhsya (Kharkov, Ukraine). T4 and T3 radioimmunoassay kits were from Minsk (Belarussia). Other chemicals used were of chemically pure grade.
90- and 720-day-old male Wistar rats, which had a free access to food and water and were kept at 24°C on a cycle of 12 h light/12 h darkness were used for experiments. The MMI was injected intraperitoneally (1 mg/100 g weight) in 0.9% NaCl to the experimental animals every day during 16 days-experiment. In some cases, the MMI-treated rats were injected intraperitoneally by T4 (200 μg/100 g weight) 48 h prior to killing. Besides, T4 (200 μg/100 g weight) was injected to the normal rats 15 and 60 min prior to killing. Control rats received 0.9% NaCl of the same volume. The animals were starved overnight prior to experiment. The thyroid state of rats was monitored by radioimmunological determination of the T4 and T3 in blood serum.
Experiments with Liver Homogenates and Slices
The 1 mCi of [14C]CH3COONa was intraperitoneally injected to rats four times every 30 minutes for 2 hours. The liver was perfused with 0.9% NaCl, then removed and washed in Krebs-Henseleit buffer, pH 7.4, containing 2 mM CaCl2 and 0.2% BSA. The pre-labeled liver was used to obtain 10% homogenates and to analyze the14C-phosphoinositides. Besides, the slices of unlabeled liver were labeled by incubation in the Eagle medium containing 10% fetal calf serum, 100 units/l streptomycin, 100 units/l penicillin, 13 mg/ml gentamycin and 0.1 μCi/ml of mio- [3H]inositol or 2.5 μCi/ml of [14C]oleic acid or 2.5 μCi/ml [3H]arachidonic acid for 1–2 h in 95% O2/5% CO2 atmosphere at 37°C. The lipids were extracted and analyzed as described below.
Cell Suspension Experiments
The hepatocytes were isolated from the liver by the method described in . Preparation of hepatocytes was started between 9:00 and 10:00 a.m. The cells (107/ml) were incubated in the Eagle medium containing 10% fetal calf serum, 100 units/liter streptomycin, 100 units/liter penicillin, 13 mg/ml gentamycin and in the presence of 100 nM NaOH (control) or L-T4(10 nM) for 30 min in 95% O2/5% CO2 atmosphere at 37°C. Before lipid extraction, the cells were washed twice with a Krebs-Henseleit buffer pH 7.4, containing 2 mM CaCl2, 25 mM HEPES, 0.1% BSA. The lipids were extracted and analyzed as described below.
Extraction and Separation of Lipids
The phospholipids were extracted according to Folch et al. , the phosphoinositides as described in . The chloroform phase was collected and dried under N2 at 37°C. The lipids were redissolved in chloroform/methanol (1:2, v/v) and applied on TLC plates. For a total phospholipids isolation, the solvent system: hexane/diethyl ether/acetic acid (80:20:2, v/v) was used, for PtdIns, PtdInsP and PtdInsP2 – chloroform/methanol/NH4OH (50:40:10, v/v). For phosphatidic acid separation the two-dimensional TLC was used. The TLC plates were developed in chloroform/methanol/ NH4OH (60:35:5, v/v) (first direction) and after – in the second direction in chloroform/methanol/acetic acid/water (80:60:7.4:1.2, v/v). The phospholipids masses were determined as described in . The gel spots containing [14C/3H]lipids were scraped and transferred to the scintillation vials. Radioactivity was measured by a scintillation counter.
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